In our laboratory, we have determined the liver specific membrane lipoprotein (LSp) antigen level in the circulating blood of the patients with viral hepatitis, SLE, and chronic renal disease by means of ELISA double sandwich method, using anti-LSp McAb of own production as the reagent. The corela-tion of LSp with the changes in HBeAg, SGPT, anti-LSpAb in the paitents' blood has also been studied. LSp was negative in the serum of normal persons, and the LSp positive rate among the patients with hepatic cell damage (autoimmune or viral hepatitis) was 48.2~70.0%. Lower positive rate (10~22.2%) was found in the patients with SLE (skin type), chronic renal disease and non-symptomatic HBsAg carriers. The difference was significant (P

Objective To investigate the effects of laparoscopic operation in treating type Ⅲ child biliary atresia and its influence on postoperative infections .Methods 85 children cases of type Ⅲ biliary atresia were randomly divided into the conventional sur-gery group(OP group ,n=42) and the laparoscopic surgery group(LP group ,n=43) .The operation situation ,postoperative changes of inflammatory factors ,changes of liver function indexes before and after operation and postoperative infection were compared be-tween the two groups .Results The operative time of the LP group was longer than that of the OP group (P<0 .05) ,the intraoper-ative blood loss and the VAS scores of the LP group were less than those of OP group (P<0 .05) ,the postoperative eating recovery time of the LP group was earlier than that of the OP group (P<0 .05) ,the length of hospital stay of the LP group was shorter than that of the OP group(P<0 .05) and the postoperative infection rate of the LP group was lower than that of the OP group (P<0 . 05) .The liver function indexes recovery showed no statistically significant differences before and in postoperative 1 ,3 ,6 ,12 months between the two groups(P>0 .05) .Conclusion The laparoscopic operation and the conventional operation in treating type Ⅲ child biliary atresia have their own advantages and disadvantages .The appropriate operation mode should be selected according to the children′s actual situation .

In this paper, we reviewed the progress in the study of the therapeutic HBV DNA vaccine, particularly on the evidences for its therapeutic effect, its mechanism of action, and to compare it with the traditional vaccines, etc. We also presented our research results on the therapeutic HBV DNA vaccine, and evaluated its clinical effect and characteristics objectively.

In this study,we constructed 2 eukaryotic expressing plasmids namely pcS2?S and pFP,encoding HBV preS2+S envelope protein and human IL 2/IFN ? fusion protein, respectively. The double plasmid fusion protein was used as an adjuvant in preparation of a treatment type HBV gene vaccine. To investigate its feasibility for use as a therapeutic DNA vaccine, we've evaluated the immune response after injection into healthy mice, HBV transgenic(Tg) mice, New Zealand rabbits and Rhesus monkeys. The results showed that the therapeutic DNA vaccine can improve: (1)the CTL activity; (2)the HBsAg(30?g/ml) specific T lymphocyte proliferation in which the stimulation index(SI) and cytokines(IL 2/IFN r) release levels of the pS2.S immunized group(SI=5.6?0.9; 226.3?41.1/51.1?7.1pg/ml) were significantly higher( P

ObjectiveTo study the diagnosis and treatment of peripelvic cysts.MethodsA view of the diagnosis and treatment of 14 cases and an evaluation of the different means of diagnosis and treatment were carried out.Results12 cases were followed up for 6～48 months post-operatively.11 cases were well recovered,with no reoccurrence or complication,and in 2 the high blood pressure dropped to normal whereas repeated urinary infection has been noted postoperatively in 1.ConclusionsB supersonic exam,KUB+IVU,CT exam are the most effective means to the diagnosis of this disease,CT exam being the first choice.Open renal cystectomy is indicated.

Pdx-1, an important transcription factor highlighting in the early pancreatic development, islet functions and pancreatic disorders, needs to be more investigated in zebrafish, and siRNA is still seldom applied in zebrafish embryo-related research. Our aim was to explore the role of pdx-1 in pancreatic development of zebrafish embryos by using siRNA approach. Microinjection, reverse transcriptase-PCR (RT-PCR), in situ hybridization and immunofluorescent staining were used in this research, and the morphology of the islet in normal zebrafish embryos, and in those treated with the siRNA specific to pdx-1 (siPDX-1) or siGFP was observed and compared. The expression of pdx-1 was detected in the stages of 1-cell, 2-cell, 4-cell, 8-cell, 16-cell, 16-hour by RT-PCT. The in situ hybridization and immunofluorescent staining results showed that siPDX-1 disturbed the formation of the islet in zebrafish embryos. Pdx-1 played multiple roles in maintaining the phenotype of the islet during embryogenesis in zebrafish.
Embryo, Nonmammalian ; Homeodomain Proteins/genetics ; Homeodomain Proteins/*metabolism ; Islets of Langerhans/cytology ; Islets of Langerhans/*embryology ; Islets of Langerhans/metabolism ; RNA Interference ; RNA, Small Interfering/*genetics ; Trans-Activators/genetics ; Trans-Activators/*metabolism ; Zebrafish

Purpose:To study the clinical significance of a tumor marker, serum CA153(sCA153), in the differential diagnosis between benign and malignant lung diseases, and in the evolution of lung cancer. Methods:124 serum samples was collected from 124 in-ward patients. Among them, 74 patients had lung cancer, and 50 patients had benign lung diseases (BLD). The concentrations of sCA153 and serum CEA (sCEA) were determined by immunoradiometric assay method.Results:In the lung cancer group, the total positive rate of sCA153 was 62.68%, which was evidently higher than that in BLD group(P0.9). In the different cell types of lung cancer patients, the concentration of sCA153 in lung adenocarcinoma patients was increased much more than that of other lung cancer groups (P

Objective:In order to obtain safer HBV DNA vaccine,recombinant kanamycin resistance eukaryotic expression plasmid containing the gene of HBV surface antigen was constructed and used to study humoral immune response in BALB/c mice.Methods:HBV antigen middle protein(preS2+S) encoding gene fragment was isolated from the recombinant eukaryotic expression plasmid pcDNA-S 2S;subcloned into eukaryotic expression vector pVAX1.After confirmed the presence of the gene by restriction endonuclease analysis,it was used to immunize the healthy BALB/c mice at different doses,and the levels of anti-HBs in serum was determined by ELISA.Results:Restriction endonuclease analysis confirmed recombinant plasmid pVAX-S 2S was what was expected.Serum anti-HBs was detected at the 2nd week after DNA vaccination at all three doses(100 ?g,50 ?g,10 ?g per mice)and their titers were increased with time.Quantitative comparison of the serum anti-HBs levels revealed significant(P

Objective To study the effects of D-Ala2-Leu5-enkephalin(DADLE) preconditioning on rabbit donor heart preservation and protective mechanism.Methods 24 rabbits were randomly divided into 4 groups. In group B, after preconditioning with DADLE(1mg?kg -1 ), the donor hearts were then arrested and preserved with Krebs-Henseleit buffer at 4℃ for 4h,and group A without conditioning as controls. Then the donor hearts were transplanted into the abdomen of recipient rabbits. Recovery situation of contraction of the donor heart was compared. The left ventricular tissues were obtained from the donor hearts after 2h of transplantation.The contents of free radicals and ATPase activity were determined and cardiac ultrastructure were observed.Results After 4h cold storage, the heart undergoing DADLE preconditioning in group B showed better recovery of left ventricular development pressure (LVDP).The activity of sodium-potassium ATPase in group B were higher than that in group A. DADLE increased free radical production 2-fold versus group A. Group B showed slighter injury in transmission electron microscopy observation than that in group A.Conclusion Our study demonstrated opioid preconditioning has protective function to rabbit heart injury caused by long term cold storage. The protective mechanism maybe related to increase of free radical content.

Objective:To investigate the value of ultrasonography in the diagnosis of cystic renal cell carcinoma(CRCC).Methods:To review and analyze 32 cases confirmed by histological examination with the complete data and to sum up their characteristic features of ultrasonographic appearance,and then compared with the results of CT.Results:The sensitivity of ultrasonography in the diagnosis of cystic renal cell carcinoma was 75.00%(24/32),CT was 87.50%(28/32),therefore there was no obvious statistical difference between them.Conclusion:Color Doppler ultrasonography has diagnostic value in cystic renal cell carcinoma.