Endometrial receptivity is the key for embryo implantation. Improving the receptivity of the endometrium so that the endometrium and the embryo tend to synchronize is the key research field of reproductive medicine in recent years. Traditional Chinese Medicine could improve endometrial receptivity from the aspects like morphology, ultrasound, hormone and receptor expression, molecular biology level, and gene level. It plays a unique role in enriching the expression of pinocytosis, improving uterine artery blood flow, and increasing sensitivity of female progesterone receptor, adjusting related factors and gene expression.

Objective To observe the effects of berberine on ICAM-1, VCAM-1 expression and inflammatory cells exudation in mice with viral pneumonia caused by influenza virus, and explore its anti-injury effect. Methods Totally 108 BALB/c mice were randomly divided into control group, model group, and berberine group. 25 μL 50 LD50 influenza virus, mouse lung-adapted strain, was intranasally inoculated to model group and berberine group. 1 h after infection, control and model group were intragastrically given 25 μL distilled water, berberine group was treated by intraperitoneal injection with berberine at a dose of 0.005 g/(kg·d) for 5 days, twice per day. On day 2, 4 and 6 after infection, immunocytochemical method was used to detect ICAM-1 and VCAM-1 expression, and sorting cell count of leukocytes in bronchoalveolar lavage fluid (BALF) were counted. Results The expression of ICAM-1 and VCAM-1 in model group increased obviously on day 2, 4, 6, and which in berberine group decreased compared with model group (P<0.01). WBC, mononuclear cell, eosinophile cell and neutrophil cell number in model group increased significantly. WBC and neutrophil cell number decreased in berberine group on day 6 (P<0.01), and the mononuclear cell number decreased on day 4 (P<0.01). Conclusion Berberine inhibited the expression of ICAM-1 and VCAM-1, and decreased the inflammatory cells exudation in lung of mice with viral pneumonia caused by influenza virus. Berberine has protective effect on inflammatory injury of lung tissue in mice with viral pneumonia caused by influenza virus.

AIM: To observe the effect of crocetin on the apoptosis and the changes of its related regulating proteins caspase-3 and Bcl-2 expression induced by hydrogen peroxide (H_2O_2) in cultured cardiomyocytes. METHODS: Changes of cellular morphology were detected under microscope. Apoptosis rates of the cells were analyzed by PI staining with flow cytometry. Expressions of caspase-3 and Bcl-2 proteins in the cells were determined by immunofluorescence with flow cytometry. RESULTS: In the concentrations used, more severe morphological changes with higher apoptosis rate of the cultured myocardial cells were seen in each H_2O_2 group than that in control group. When treated with 1?10 -4 mol?L -1 H_2O_2, the caspase-3 was increased and Bcl-2 protein decreased remarkably in the cells. But each dosage of crocetin, especially the highest one (5?10 -5 mol?L -1, P

Objective:To observe the impact of the medicated serum of Shumai Capsule and its decomposing on vascular smooth muscle cells(VSMC) proliferation induced by angiotensinⅡ(AngⅡ) and level of NO,SOD,MDA in cell culture supernatant.Methods:Tissue explant method was used for cultivating vascular smooth muscle cells,serum pharmacology was used,AngⅡ10-7mol/L as a stimulating factor,medicared serum divided into Shumai Capsule group,Huoxue Huayu decomposing group(group 1) and Bupi Yishen decomposing group(group 2),MTT colorimetric was used to test OD values,biochemical detection kit was used to test NO,SOD,MDA levels.Results:The OD value of Shumai Capsule and group1 had significant difference from AngⅡ group and group2(P

ObjectiveTo evaluate the effects on serum homocysteine(Hcy) level,vascular function and carotid intima-media thickness(IMT) in metformin-treated diabetic patients with or without supplementation with folate and Vitamin B12.MethodsA total of 100 newly diagnosed diabetic type 2patients were divided into two groups by random digits table with 50 cases each,90 patients completed study.Forty-seven participants (control group) received a 6-month course of metformin treatment,43 patients (treatment group) received mefformin,folate and Vitamin B12 treatment.The levels of serum Hcy,endothelin-1 (ET-1),carotid IMT,large or small arterial elasticity index (C1,C2),flow-mediated dilatation (FMD) of the brachial artery were evaluated before and after treatment.ResultsThe level of serum Hcy in control group significantly increased compared with before treatment[ (13.4 ± 2.7)μ mol/L vs.(11.1 ± 1.9)μ mol/L],hut the level of serum Hcy in treatment group significantly decreased compared with before treatment [ (9.2 ± 1.8 ) μ mol/L vs. ( 11.3 ± 2.0) μ mol/L ],there was significant difference(P ＜ 0.05 ).A beneficial effect was observed in the serum ET-1,FMD,carotid IMT and C2 in treatment group[ (20.0 ± 6.2)ng/L,( 15.8 ± 7.6)％,(0.8 ± 0.2) mm,(4.1 ± 2.1 ) ml/mm Hg ( 1 mm Hg =0.133 kPa) × 100 vs. (31.3 ±10.1 ) ng/L,(9.7 ± 4.5)％,( 1.1 ± 0.4) mm,(2.3 ± 1.0) ml/mm Hg × 100 ] (P ＜ 0.05).The levels of ET-1,FMD,carotid IMT and C2 after treatment in control group [ (24.8 ± 6.8) ng/L,( 12.9 ± 6.3 )％,(0.9 ± 0.3)mm,(3.0 ± 1.4) ml/mm Hg × 100] had significant difference compared with before treatment [ (30.6 ± 8.7)ng/L,(9.8 ± 4.6)％,( 1.0 ± 0.3) mm,(2.2 ± 0.9) ml/mm Hg × 100](P＜ 0.05).However,the results were improved significantly in treatment group than those in control group (P ＜0.05).In treatment group,significant correlation were detected between changes of Hcy and ET- 1 (r =0.43,P ＜ 0.05 ),carotid IMT(r =0.56,P ＜ 0.05),FMD (r =-0.54,P ＜ 0.05 ),C2 (r =-0.37,P ＜ 0.05 ).ConclusionsAdministration of folate and Vitamin B12 can reduce the levels of serum Hcy and ET-1 in metformin-treated type 2 diabetic patients,which exert beneficial effect on carotid IMT,FMD and C2.

Objective To explore the value of bone marrow biopsy ( BMB) in early diagnosis of aggressive NK cell leukaemia( ANKL). Methods The clinical data of ten cases with ANKL were retrospectively analyzed,morphology, immunophenotype and hybridization in situ of bone marrow were analyzed. Results In all cases, BMB showed hypercellular, with 4 cases markedly hypercellular. Atypical neoplastic cells demonstrated focal and fascicle growth pattern and were composed of median-sized cells with a few cytoplasm, slightly irregular nuclei, fine chromatin, indistinct nucleoli and some mitotic counting. Characteristic histocytes with haemophagocytosis were observed in the bone marrow slides. The neoplastic cells were positive for CD2、CD3e、CD7、CD56、TIA-1. EBER was found positive in all cases. Conclusion Bone marrow biopsy and immunochemistry were essential and reliable diagnostic tool in early diagnosis of ANKL.

OBJECTIVETo investigate the therapeutic effects of Ginkgo biloba extract (EGb) in rats with acute myocardial injury induced by isoproterenol (ISO).

METHODThe rats, induced by Isoproterenol (4 mg x kg(-1) x d(-1), 10 d, sc), were divided into groups: sham, model, metoprolol (10 mg x kg(-1) x d(-1), 13 d, ig), EGb (100 mg x kg(-1) x d(-1), 13 d, ig).

RESULTThe cardiac parameters of the Model group were compromised significantly in both systolic and diastolic function. Improvement in cardiac function by EGb was significant. In model groups, plasma activities of AST, LDH, CK, HBDH, CKMB and ventricular weight index (LV and RV/BW) were elevated significantly. With the treatment with EGb and metoprolol, the enzymes and ventricular weight index were significantly ameliorated.

CONCLUSIONG. biloba extract was beneficial to cardiac performance by improving myocardium enzymes and cardiac function in isoproterenol induced myocardial injury in rats.

Animals ; Drugs, Chinese Herbal ; therapeutic use ; Ginkgo biloba ; chemistry ; Hemodynamics ; drug effects ; Isoproterenol ; adverse effects ; Male ; Myocardial Ischemia ; chemically induced ; drug therapy ; enzymology ; physiopathology ; Organ Size ; drug effects ; Rats ; Rats, Sprague-Dawley

Objective To investigate the early physiologic and pathologic changes of primary explosive injury to brain in dogs.Methods Thirty mongrel male dogs were randomly divided into 13 mm detonated injury group (n=10),9 mm detonated injury group (n=10) and 5 mm detonated injury group (n=10); a spherical explosive with explosive energy of 1 g TNT was used to detonate at different distances (13,9 and 5 mm) to dogs' head to cause the severe,moderate and slight degrees of craniocerebral primary explosive injury in these dogs,respectively.Changes of respiratory frequency (RF),heart rate (HR) and mean arterial blood pressure (MABP),survival time and behavior in these dogs were observed; HE staining and electron microscopy were used to observe the pathological and ultrastructural changes of the brain tissues.Results The RF,HR and MABP levels after cerebral explosive injury were decreased in dogs of three groups; the decreased extent was increased following the increase of injury extent,with significant difference among the three groups (13 mm detonated injury group＞9 mm detonated injury group＞5 mm detonated injury group,P＜0.05).The server the injury,the more obvious the changes of dog behaviors (13 mm detonated injury group＞9 mm detonated injury group＞5 mm detonated injury group,P＜0.05).There were different extents of contusion and laceration of the brains,brain hemorrhage,and subdural hematoma in the three groups.Conclusions RF,HR and BP levels are decreased in three groups after cerebral primary explosive injury in dogs; the more obvious the physiologic and pathologic changes,the severer degrees of craniocerebral explosive injury.The severer injury dogs have more obvious behavioral changes.The survival time in dogs with the severer degrees of craniocerebral explosive injury is shorter than that in slight injury one.

Objective To establish new experimental models of primary explosive injury to brain in dogs and investigate the effect of early treatment on primary explosive injury in dogs by using two doses of 20％ mannitol.Methods Thirty-six mongrel male dogs were randomly divided into three groups: model group, high-dose treatment group and low-dose treatment group (n=12).Models of primary craniocerebral explosive injury were established in all the groups.Dogs in the high-dose treatment group and low-dose treatment group were given 1.0 g/kg and 0.5 g/kg 20％ mannitol 6 h after injury, and these treatments were given every 6 h;0, 3, 6, 12, 24 and 48 h after injury, respiratory frequency, heart rate, blood pressure (BP), intracranial pressure (ICP), CT of skull, urea nitrogen (BUN), creatinine (Cr) and survival time were observed and compared.Results The levels of respiratory frequency and heart rate were significantly increased, and the levels of BP and ICP were significantly decreased in the high-dose treatment group and low-dose treatment group as compared with those in the model group 12, 24 and 48 h after injury (P＜0.05);24 and 48 h after injury, as compared with those in the model group and high-dose treatment group, the levels of BUN and Cr in the low-dose treatment group were significantly lower (P＜0.05).The midline shift in the high-dose treatment group ([3.5±0.41] mm) and low-dose treatment group ([3.3±0.22] mm) was significantly decreased than that in the model group ([6.4±0.50] mm) 48 h after injury (P＜0.05).The survival time in both high-dose treatment group (131.6±8.73 h) and low-dose treatment group (133.7±9.31 h) was significantly longer than that in control group (96.0±3.0 h,P＜0.05).Conclusion Early proper treatment for primary craniocerebral explosive injury by using mannitol can relieve the indexes of respiratory frequency, heart rate, BP and ICP, and relieve the severities of cerebral edema, prolong the survival time in dogs, but high-dose 20％ mannitol might aggravate the kidney damage of the injured dogs.