Objective:To investigate the influences of laparoscopic surgery on Th1/Th2 balance in patients with uterine myomas.Methods:In a prospective study,the number of the Th subsets,Th1/Th2 ratio,the serum level of IL-18 and IL-10 in 20 patients submitted to laparoscopic operation and 20 patients undergoing conventional open operation were evaluated preoperatively as well as 2,24,48 h postoperatively.Results:The level of Th1 cell,IL-18,and the Th1/Th2 ratio decreased significantly (laparoscopic group: P

Incidence of thyroid cancer has continuously increased in the last three decades globally. A similar trend of thyroid cancer incidence appeared in China. New cases and deaths of thyroid cancer in China accounted for 15.6% and 13.8% of the global new cases and deaths in 2012. The incidence in East China was the highest while that in Middle China was the lowest. Thyroid cancer was more common in urban areas than in rural counterparts. The incidence was substantially higher in female patients than male. Thyroid cancer occurred most commonly in middle age. Radiation exposure, iodine suffciency or deifciency, sex hormone and genetic mutations were risk factors for thyroid cancer. To prevent it, potassium iodide should be taken to prevent radiation fallout in nuclear accidents, unnecessary exposure to radiation from medical imaging is reduced, iodine surveillance system is improved and appropriate standards for iodized salt are established.

Objective:To evaluate the possible roles of IL-18 in HBV infection, and to clarify the immune state during IFN? therapy. Methods:Peripheral blood lymphocytes from 2 groups of HBV infectors(including 15 asymptomatic carriers,26 patients with chronic hepatitis B) and 10 healthy individuals (as normal controls) were analyzed by flow cytometry. The levels of IL-18 were also detected before, during, and at the end of IFNa therapy,Results: (1)The level of IL-18 was the lowest in asymptomatic carrier group. However there was no significant difference between chronic hepatitis and normal control. (2)The patients with chronic hepatitis were further divided into 4 subgroups according to the inflammation degree of liver. Results showed that the severity degree of liver inflammation was accompanied with the elevation of IL-18 production. (3)IL-18 in chronic hepatitis B correlated with serum ALT positively ( r = 0.61,P

Objective To investigate the liver function of patients with chronic liver diseases undergoing gynecologic laparoscopic operations.Methods Twenty-six asymptomatic HBV carriers,28 mild chronic hepatitis,21 compensatory cirrhosis were assigned to undergo gynecologic laparoscopic operations.Liver function were tested and compared in these three groups before operation and on the 1st、3rd、7th day after operation.Results 1st day after operation,ALT and AST in asymptomatic carriers and mild chronic hepatitis groups were significantly higher than preoperation ( asymptomatic carriers:ALT P ＜ 0.05,AST P ＜ 0.05 ; mild chronic hepatitis:ALT P ＜ 0.05,AST P ＜ 0.05 ).Then they recovered on the 3rd day after operation ( asymptomatic carriers:ALT P ＞ 0.05,AST P ＞ 0.05 ; mild chronic hepatitis:ALT P ＞ 0.05,AST P ＞ 0.05 ).ALB,total bilirubin (Tbil) after operation were not significantly different from those before operation on every tested day in these two groups ( asymptomatic carriers:ALB P ＞ 0.05,Tbil P ＞ 0.05 ; mild chronic hepatitis:ALB P ＞ 0.05,Tbil P ＞ 0.05 ).In compensatory cirrhosis group,ALT,AST,Tbil were significantly higher on the 1st day,3rd day after operation than preoperation ( 1st day:ALT P ＜0.01,AST P ＜0.01,Tbil P ＜0.01 ;3rd day:ALT P ＜ 0.05,AST P ＜ 0.05,Tbil P ＜ 0.05 ).ALB levels were significantly lower on the 1st day,3rd day after operation than preoperation ( 1st day:P ＜0.01 ;3rd day:P ＜0.05 ).All the indexes recovered to the levels of preoperation on the 7th day after operation( ALT P ＞ 0.05,AST P ＞ 0.05,Tbil P ＞ 0.05,ALB P ＞0.05).Conclusions To some extent,gynecologic laparoscopic operations have some adverse effects on short-term liver function after operation in patients with chronic liver diseases.Compensatory cirrhosis patients present delayed recovery compared with asymptomatic carriers and mild chronic hepatitis patients.

AIM　This paper was to review the methods of establishing experimental animal models of intrauterine growth retardation (IUGR) and their observed index. METHODS　Passive smoking, passive smoking plus drinking wine, NG nitro-L-arginine methyl ester, dactinomycin, thiamine deficiency plus pyrithiamine, or ligating the uterine artery during pregnancy were used.RESULT　The fatal and maternal body weight was lower body length; was shorter; infant physique and nerve system development was slow.CONCLUSION　The above methods could establish animal models of IUGR.

Objective To investigate the effect of continuous nursing on the psychological status and quality of life in patients with low rectal cancer after Miles operation.Methods A total of 84 patients with low rectal carcinoma underwent Miles operation were selected from August 2014 to August 2016 in Henan Rongjun Hospital.The patients were divided into observation group (n =38) and control group (n =46) according to nursing measures.The patients in the control group were given routine nursing measures,while the patients in the observation group were given continuous nursing measures on the basis of routine nursing care.The psychological status and quality of life of the patients were compared between the two groups before and after intervention.Results There was no significant difference in swlf-rating anxiety scale (SAS) and self-rating depression scale(SDS) scores between the two groups before intervention (P ＞ 0.05).After six months intervention,the SAS and SDS scores in the two groups were significantly lower than those before intervention,and the scores of SAS and SDS in the observation group were significantly lower than those in the control group (P ＜0.05).There was no significant difference in the quality of life between the two groups before intervention (P ＞ 0.05).After six months intervention,the quality of life of patients in the two groups were significantly higher than those before intervention,and the quality of life of patients in the observation group were significantly higher than those in the control group (P ＜ 0.05).Conclusion Continuous nursing can significantly improve the negative emotions of anxiety and depression,and improve the quality of life in patients with low rectal cancer after Miles operation.

We first report the pharmacokinetics of paeoniflorin ( PF), that is a main component of paeony roots, in dogs by HPLC analysis. The experimental results were shown that after i .v . of 11.25 mg/kg PF in 4 dogs, the curve of plasma concentrations versus times for PF was fitted well to a open 2 compartment model, the T1/2 (?) was 6.29?1.80min T1/2 ( ? ) was 133.41?84.89 min, VB was 0.54 ? 0.10 L/kg, andCL was 3.41 ? 1.01ml/min?kg-1 .The results also were shown that PF was rapidly removed from the blood by kidney and the accumulated recovery amounts of excretion was 36.85% and 79.33% of total i .v . doses during 20 min and 7h administrations respectively. The elimination of PF by liver was lower than by kidney, and the accumulated amount of PF in bile was only 3.77% within 7h after iv.

Aim To discover lead compound whose function is similar to EPO.Methods A high-throughput Fluorescent Polarization(FP) assay was established for screening ligand of EPO receptor from small molescules and natural products.In the FP assay,EPO receptor was abstracted from FVA cell.EPO was labeled with FITC.Results The optimized concentration of FITC-EPO was 10~(-9) mol?L~(-1).Different concentrations of DMSO(VDMSO/Vtotal: 0%,0.5%,1%,2.5%,5%) didn′t affect the FP assay.The value of Z′ in the FP assay was 0.78.Conclusion The assay was robust and of high sensitivity.

Objective To explore the prevalence of anemia,iron deficiency and iron deficient anemia (IDA),and to identify their risk factors in 3 to 6 months infants in Dongcheng District of Beijing.Methods All the 845 infants aged 3 to 6 months who received bacille calmette guerin (BCG) vaccine during December 2010 and October 2011 in Hepingli Hospital of Beijing were invited to complete the investigation.Data (months,gender,birth weight,birth height,etc) were collected through a questionnaire survey.Infants were tested for hemoglobin (Hb),mean corpuscular volume (MCV),mean corpuscular hemoglobin (MCH),mean corpuscular hemoglobin concentration (MCHC),serum ferritin (SF) and zinc protoporphyrin (ZPP) and mothers underwent measurement for Hb,SF and serum iron (SI).Descriptive epidemiology and non-conditional logistic regression model were used to analyze the prevalence of nutritional anemia.Results The prevalence of anemia,iron deficiency and IDA was 12.06％,7.38％ and 2.11％,respectively.Non-conditional logistic regression analysis showed that the associated factor of anemia in infants was month age (vs.3-4 months age group,4-5 months age group:odds ratio (OR) =0.496,95％ confidence interval (CI) 0.272-0.905 ; 5-6 months age group:OR =0.494,95％ CI 0.234-1.043).The factors associated with iron deficiency were month age (vs.3-4 months age group,4-5 months age group:OR =0.539,95％ CI 0.266-1.088 ; 5-6 months age group:OR =0.334,95％ CI 0.125-0.891) and time of first breast-feeding (vs.the first day,the second day:OR =2.359,95％ CI 1.191-4.675; the third day:OR =1.154,95％ CI 0.450-2.963).Conclusions Our data show that iron nutrition in 3 to 6 months-old infants in Dongcheng District of Beijing was in good situation.The influencing factors of iron nutrition in infants may be month age and the time of first breast feeding.

AIM To establish mice hyperuricemia model METHOD Yeast extract paste 7 5～30 g?kg -1 was given to mice by ig once daily for 7,14,21,28 consecutive days, and detected the level of uric acid in serum RESULT The serum uric acid level in mice were (271 8?53 2),(215 4?31 5),(195 9?56 0),(142 1?30 7) ?mol?L -1 in 30 g?kg -1 group after administration 1,2,3,4 weeks;and (226 8?40 7),(148 67?30 4),(176 9?27 0),(119 3?27 4) ?mol?L -1 in 15 g?kg -1 group after administration 1,2,3,4 weeks;In 7 5 g?kg -1 group the serum uric acid level was (117 0?29 0) ?mol?L -1 after 1 week, respectively CONCLUSION Yeast extract paste (15～30) g?kg -1 given by ig could form mice hyperuricemia model, and the hyperuricemia could last 1～2 weeks