BACKGROUND:Recently,in vitro nucleus gelatinosus and annulus fibrosus cell culture technology,especially cartilage tissue engineering development and primary success in autogenous intervertebral disc cell transplantation to repair nucleus pulposus defect,brings a hope for regeneration and reparation of degenerated intervertebral disc.OBJECTIVE:To explore the dose-dependent and time-dependent effects of insulin-like growth factor-Ⅰ(IGF-Ⅰ) on proliferation of anulus fibrosus cells in vitro.DESIGN,TIME AND SETTING:Single-sample observation was performed at the Laboratory of Parasite,Shanxi Medical University between September 2006 and January 2007.MATERIALS:Thirty 1-month-old Wistar rats,irrespective of gender,were selected.METHODS:Anulus fibrosus cells were isolated and cultured in vitro.In dose-dependent test,IGF-Ⅰ(0.1,1.0,10,and 100 ?g/L),prepared by volume fraction 0.01 or 0.1 calf serum HAMF-12,were added.Cells not with IGF-Ⅰ served as control.Cells were cultured for 72 hours.In time-dependent test,optimal dose of IGF-Ⅰ containing volume fraction 0.1 calf serum and F-12 solution was added,and cells not with IGF-Ⅰ served as control.The cells were cultured for 1,3,5,and 7 days.MAIN OUTCOME MEASURES:Cell biological features were detected using HE staining and toluidine blue immunohistochemical staining;Dose-dependent and time-dependent effects were examined by MTT assay.RESULTS:HE stained cells were fusiform-shaped with pseudopodia,round or oval nucleus.The cytoplasm was blue after stained with the toluidine blue.The immunohistochemical test revealed that there existed positive expression of typeⅠcollagen in the cells.In the presence of 10% calf serum,IGF-Ⅰ significantly improved cell proliferative activity in a dose-dependent manner within effective dose range.CONCLUSION:IGF-Ⅰ can stimulate cell proliferation of rat anulus fibrosus cells in vitro in a dose-dependent and time-dependent manner.

ObjectiveTo discuss the efficacy of Williams Life Skills Training （WLST） in depressive adolescents with non-suicidal self-injury （NSSI）. MethodsA total of 88 depressive adolescents with NSSI hospitalized in Suzhou Guangji Hospital from January to June 2019 were selected and grouped according to admission order. The corresponding random number was even in intervention group （n=44） and odd as control group （n=44）. Both groups received a 4-week routine depression care， based on this， intervention group received WLST. All selected individuals were assessed using Hamilton Depression Scale-17 item （HAMD-17）， General Self-Efficacy Scale （GSES） and adolescent student life satisfaction scale at the baseline and end of treatment. Then the incidence of NSSI behavior during hospitalization and the reduction rate of HAMD-17 score at discharge were compared between the two groups. ResultsThe incidence rate of NSSI behavior during hospitalization showed significant difference between groups （χ²=11.702， P=0.001）. HAMD-17， GSES and satisfaction scores at discharge were significantly different from those at admission （t_{control group}=-5.256， 10.690， -21.220； t_{intervention group}=-12.540， 11.300， -32.840， P<0.01）. HAMD-17， GSES， satisfaction scores and the reduction rate of HAMD-17 score also showed significant differences between groups （t=0.851， -12.809， -4.883， χ²=75.990， P<0.05 or 0.01）. ConclusionApplication of WLST in depressive adolescents with NSSI may reduce the incidence rate of NSSI behavior， alleviate the degree of depression， enhance the sense of self-efficacy， and improve life satisfaction.

Glioma-infiltrating lymphocytes (GIL) were isolated from 9 surgical biopsy specimens of primary brain gliomas using mechanical and enzymatic digestion and discontinuous density gtadient centrifugation. During cultured in the presence of interieukin-2 (IL-2) for a period of four weeks, GIL were expanded 48.4-fold on the averags, even up to 118-fold. GIL activated by IL-2 had specific cytorytic activity against autologous glioma cells. Analysis of T subsets of GIL freshly isolated showed that CD3+ cells were 71.0?11.9%, CD4+ cells 34.2?6.1% and CD8+ cells 37.0?7.6%. Ability of activated GIL to secrete ?-interferon (?-IFN) was significantly higher than that of freshly isolated GIL and autologous peripheral blood lymphocytes (PBL). The results suggest that GIL have many advantages for an adoptive immunotherapy of patients with brain gliomas and is a new type of antitumor immune effector.

Objective To evaluate the efficacy and safety of anticoagulant therapy with warfarin for hemodialysis patients with atrial fibrillation.Methods A cohort of 118 hemodialysis patients with atrial fibrillatio were enrolled.The patients were divided into two groups:in warfarin group (n=53) the standard medication of warfarin was given for at least 3 months by adjusting the INR between 2.0 and 3.0;while no anticoagulants were given after discharge in non-warfarin group (n=65).Patients were followed up regularly,the events of stoke,death and bleeding were documented and compared between two groups.Results There were no significant differences in age,sex,underlying disease,predictive score of stroke risk in patients with non-valvular atrial fibrillation (CHA2DS2-VASc),predictive score of bleeding risk in patients with non-valvular atrial fibrillation (HAS-BLED) and dialysis frequency between the two groups (P＞0.05).There were no significant differences in events of ischemic stroke [11％(6/53) vs.12％(8/65),x2=0.027,P=0.87],bleedings [(9％(5/53) vs.5％(3/65),P=0.46] and gastrointestinal bleeding events [6％(3/53) vs.3％ (2/65),P=0.66] between two groups.There were no significant differences in the death event [15％(10/53) vs.22％(14/65),x2=0.129,P=0.72] and cardiac death [9％(5/53) vs.11％(7/65),x2=0.057,P=0.81] between two groups.Conclusion This study suggests that warfarin may not prevent ischemic stroke in hemodialysis patients with chronic atrial fibrillation,further studies are needed to determine the risks and benefits of anticoagulation therapy in these patients.