Objective:To explore the effects of IL-1 beta on the differentiation of NSCs into dopaminergic neurons.Methods: To isolate and culture mesencephalic neural stem cells in vitro.we used immunocytochemistry to detect TH-positive neurons induced by 10%FBS and interleukin-1 bate for 7days respectively.Results: The positive ratio of TH expression in induced cells by 10%FBS,IL-1? 10pg/ml,IL-1? 120pg/ml,IL-1? 150pg/ml,IL-1? 200pg/ml were 0.45%,0.6%,7.2%,12.5%and 8.75% respectively.The positive ratios of TH expression in induced cells by IL-1? 150pg/ml(12.5%) were much higher than that by 10%FBS(control 0.45%)(P

Objective To study the effect of posterior unilateral open-door laminoplasty which anchor the different segments of patients on neurological function improvement rate,cervical range of motion,axial symptoms and complications.Methods From June 2009 to April 2013,86 patients with cervical spondylotic myelopathy received posterior unilateral open-door laminoplasty by anchoring were selected.The open segments were C3-7.They were divided into two groups according to the anchor segments.Group A of 48 patients,anchor segments was C3-7,using 5 anchoring nails.Group B of 38 patients,anchor segments was C3,5,7,using 3 anchoring nails.The improvement rate of nerve function,ranges of neck motion,incidence of axial symptoms and postoperative complications were compared between two groups.Results The improvement rate of nerve function,loss of ranges of neck motion and incidence of axial symptoms between group A and group B had no significant difference[(56.4 ± 18.3)％ vs.(56.8 ± 19.6)％,(9.27 ± 5.42)° vs.(9.06 ± 4.89)°,22.9％ (11/48) vs.23.7％ (9/38)] (P ＞ 0.05).Two groups of patients with postoperativefollow-up were not found door re-clousure phenomenon.Conclusions In the anchor posterior unilateral open-door laminoplasty,they have same improvement rate of neural function on anchoring 3 segments and anchoring 5 segments.The postoperative complications are not increased,but the cost of internal fixation is decreased,operation become more economical.

Objective To compare the expression of myocardial connexin 43(Cx43)mRNA and its protein during early and late cardioprotection stages of exercise preconditioning.Methods Thirty-two Sprague-Dawley rats were randomly divided into a control group,an exhaustive exercise(EE)group,an early exercise preconditioning + exhaustive exercise(EEP+EE)group and a late exercise preconditioning + exhaustive exercise (LEP+EE) group,each of 8.All groups were given intervention as their group name indicated.Then in situ hybridization and real-time fluorescent quantitative PCR methods were used to detect the changes of myocardial Cx43mRNA and immunohistochemical method and Western blotting were used to detect the changes of Cx43 protein.Results Compared with EE group,there was significant increase in Cx43 mRNA and its protein expression in group EEP+EE and LEP+EE.Compared with EEP+EE group,no significant changes was found in situ hybridization and Cx43 Immunoreactivity in LEP+EE group,neither did significant differences in the expression of Cx43 mRNA and its protein.Conclusion EEP and LEP can significantly promote the expression of myocardial Cx43 mRNA and its protein respectively.However there is no significant changes of myocardial Cx43 mRNA and protein expression between the 2 time phases.It demonstrates that the expression of Cx43 in the early and late stage of myocardial protective effect was consistent with the changes of the early and late phase of the protective effect of EP.

OBJECTIVE:To establish a HPLC method for the determination of aristolochic acidⅠand aristolochic acid Ⅱin human serum.METHODS:The separation was performed on C 18 column(250mm?4.6mm,5?m).The mobile phase consisted of methanol-water-acetic(72∶27∶1)at a flow rate of1.0ml/min.The detection wavelength was250nm.RESULTS:The linear ranges for aristolochic acidⅠand aristolochic acid Ⅱ were0.84～13.50?g/ml(r=0.9997,n=5)and2.03～32.50?g/ml(r=0.9994,n=5),respectively.The lowest determination concentration of aristolochic acid Ⅰ and aristolochic acid Ⅱ were0.3?g/ml and0.1?g/ml,respectively.CONCLUSION:The method is simple and practicable,and it can provide basis for clinical diagnosis and treatment of aristolochic acid nephropathy.

Objective To study MRI manifestation of the non-diver bone infarction. Methods Six cases of non-diver bone infarction involved 18 bones totally, in which 15 bones were confirmed by surgical operation and pathology. All cases were examined by MRI through T 1WI, T 2WI, PDWI, FLASH T 2WI, and by X-ray plain film. Result (1) Major MRI manifestation was moderate signal intensity on T 1WI and inhomogenous high signal intensity on T 2WI in the centers of the foci whose margin were rugged and rough bands which were low signal intensity on T 1WI and two layers on T 2WI. (2) MRI manifestation was atypical when focus was small (

To study the effects of MRF4 transfection on differentiation and expression of myogenic regulatory factors of human rhabdomyosarcoma RD cells, the plasmid-MRF4 cDNA was transfected into cultured rhabdomyosarcoma RD cells with lipofectin method. The myogenic regulatory factors MRF4 and MyoD mRNA were measured with in situ hybridization and the expressions of myosin heavy chain(MHC) and a-actin in the cells were assayed with immunocytochemical method. The cell growth and morphology were observed at the same time. It was found that the morphology of differentiation increased and the growth was suppressed in RD cells after transfection. The expression of MHC and a-actin were significantly increased in RD cells after transfection, while the expressions of MRF4 and MyoD mRNA were up-regulated. It is suggested that transfection of MRF4 can induce differentiation of RD cells and up-regulate the expression of MyoD.

Objective To look for a method for isolation and cultivation of mesenchymal stem cells from human adipose tissue in vitro. Method The adipose tissue was obtained from the omentum of abdominal surgery patients under the aseptic condition. The fat then was minced and digested with 5ml 0 25% trypin for 30 minutes in a 37℃ water bath under constant agitation. The layer with monoclear cells was aspirated and supplemented with albumin, then was cultured in DMEM with 15% calf serum. Immunocytochemistry was used to determine the surface molecule CD44, HLA DR and VWF. Result There was a large amount of mesenchymal stem cells in human adipose tissue. Immunocytochemical staining showed that most of the cultured cells were CD44 positive, few HLA DR positive and VWF positive, indicating that most of the cultured cells were MSC, and the others were fibroblasts and endothelial cells. Conclusion A simple and convenient method to isolate and culture the adipose tissue derived mesenchymal stem cells was successfully established, providing the foundation for the future use of ADMSCs in the treatment of cardiovascular diseases.

Objective To evaluate the clinical value of retroperitoneal laparoscopic and open radical nephrectomy.Methods Clinical data of 12 cases of retroperitoneal laparoscopic radical nephrectomy(Retroperitoneal Group) and 13 cases of open radical nephrectomy(Open Group) from March 2004 to March 2005 in this hospital were retrospectively analyzed and compared in respect of operation time,hemorrhage volume in operation,postoperative hospitalization,and occurrence of complications.Results The two groups of procedure were successfully completed.The operation time was significantly longer in the Retroperitoneal Group(111.1?20.9 min) than in the Open Group(90.1?10.2 min)(t=3.234,P=0.004).The intraoperative hemorrhage volume was significantly less in the Retroperitoneal Group(96.3?15.5 ml) than in the Open Group(150.0 ?25.8 ml)(t=-6.240,P=0.000).The length of postoperative hospital stay was significantly shorter in the Retroperitoneal Group(8.3?2.0 d) than in the Open Group(11.7?2.2 d)(t=-4.031,P=0.000).Conclusions Retroperitoneal laparoscopic radical nephrectomy has advantages oves open nephrectomy in intraoperative hemorrhage volume and postoperative recovery,but operation time is longer and the cost is more expensive.

AIM: To explore the immunotheraptical drugs for multi-drug resistant pulmonary tuberculosis (MDR-TB). METHODS: 79 patients with MDR-TB were randomly assigned to two groups. 46 cases in M.vaccae group were treated with "3AkPaThLevL/15PaThLevL" and M. vaccae, and 33 cases in control group were treated only with "3AkPaThLevL/15PaThLevL". The clinical effect and T-lymphocyte subsets in patients were observed after being treated for 1,2 and 3 months, respectively. RESULTS: The sputum negative rates ((41.3)%, (63.0)%, and (80.4)%) and the X-ray resolution rates ((30.4)%, (50.0)%, and (67.4)%) of the M.vaccae group were superior to those ((12.1)%, (27.3)%, and (39.4)%, P

Objective To investigate the pathological features of airway remodeling and evaluate interventional effects of nerve growth factor (NGF) on the expression of matrix metalloproteinase-9 (MMP-9), an important mediator of airway remodeling, in guinea pig asthma model. Methods Guinea pigs were randomly divided into the control group, asthma group, and antibody NGF intervention group (each group had 8 guinea pigs). In the asthma group the animals were sensitized by repeated exposure to aerosolized ovalbumin combined with Al(OH)_3. The thickness of the smooth muscle of intrapulmonary bronchi was measured by image analysis system. The expression of MMP-9 in bronchi and lung tissues were assayed with immunohistochemistry combined with the micro-image analysis system. The levels of MMP-9 mRNA in bronchi and lung tissues were determined by RT-PCR. Results After repeated allergen challenge, obvious infiltration of inflammatory cells and proliferation of goblet cells and smooth muscle were demonstrated in guinea pig bronchi. Expression levels of MMP-9 in the epithelial cells of bronchi were significantly higher in asthmatic animals than those of control group animals. Compared with asthmatic group, there was mild inflammation reaction, and decrease in collagen deposition and expression of MMP-9 in antibody NGF group animals, and they were not significantly higher than that in control group animals. Conclusions Repeated exposure of allergen induced airway inflammation and remodeling. MMP-9 plays an important role in airway remodeling. Antibody NGF intervention could inhibit airway remodeling through inhibition of the expression of MMP-9.