To investigate the level of serum IL-10 in allergic rhinitis patients and the correlation between IL-10 and serum total IgE (TIgE). 50 allergic rhinitis patients and 30 normal subjects were involved in the study. The levels of serum IL-10 and TIgE were measured by enzyme-linked immunosorbent assay (ELISA), and the correlation between serum IL-10 and TIgE was analyzed. In the allergic rhinitis group, the levels of serum IL-10 and TIgE were 8.34 +/- 2.48 pg/mL and 142.6 +/- 28.2 KU(A)/L. In the normal control group, they were 12.86 +/- 2.88 pg/mL and 47.2 +/- 12.2 KU(A). There were significant differences between the two groups (P < 0.01); and the level of serum TIgE in the patients was negatively correlated with that of IL-10 (r = -0.46, P = 0.02). The level of serum IL-10 was significantly decreased in allergic rhinitis patients, which was beneficial to the synthesization of IgE. IL-10 plays an important role in the episode of allergic rhinitis.
Enzyme-Linked Immunosorbent Assay ; Immunoglobulin E/*blood ; Interleukin-10/*blood ; Rhinitis, Allergic, Perennial/*blood

Hyperglycemia is considered to be a predictor of death, poor functional outcome, symptomatic intracerebral hemorrhage, and increased infarct volume in patients with acute ischemic stroke. Many studies have used parameters such as standard deviation, coefficient of variation, blood glucose level, stress hyperglycemia ratio, and glucose/glycated hemoglobin ratio to express hyperglycemia, and then study the relationship between hyperglycemia and stroke outcome. In patients with stroke receiving intravenous thrombolysis, hyperglycemia is closely associated with symptomatic intracerebral hemorrhage, poor neurological outcome, and lower vascular recanalization rate. In patients with stroke undergoing endovascular mechanical thrombectomy, hyperglycemia is closely associated with poor long-term outcome, increased mortality, and increased infarct volume. However, whether hyperglycemia will affect the effect of reperfusion therapy in acute ischemic stroke remains controversial. The possible mechanisms of hyperglycemia leading to poor outcomes after reperfusion therapy in patients with ischemic stroke include inhibition of vasodilation, reduction of plasma fibrinolytic activity, destruction of the blood-brain barrier, promotion of oxidative stress, and reduction of ischemic penumbra volume. Studies have shown that for stroke patients with hyperglycemia, intensive blood glucose control does not bring significant benefits. Therefore, the blood glucose control program of patients with acute ischemic stroke and hyperglycemia needs further study and discussion.

Objective To study the prognosis value of the expression of vascular endothelial growth factor and it's acceptor(VEGF,Flk-1) and microvessel density(MVD) gene proteins in the breast carcinoma and paracarcinoma tissue.Methods Immunohistochemical technique was adopted to detect the protein expression of VEGF,Flk-1 and MVD in 76 cases of breast carcinoma and 10 cases paracarcinoma tissue.Results (1)The positive expression of VEGF,Flk-1 were 59.3% and 61.3% in the carcinoma group,which were higher than in the paracarcinoma tissue(P

OBJECTIVETo assess the association between polymorphism of methylenetetrahydrofolate reductase (MTHFR) gene C677T locus and nonsyndromic cleft lip with or without cleft palate (NSCL/P) in Shanxi Province of China.

METHODSBlood samples from 150 patients and their parents and 150 controls and their mothers were collected. The polymorphism of MTHFR gene C677T locus were analyzed by the methods of polymerase chain reaction and restriction fragment length polymorphism technique(PCR-RFLP). Case-control analysis, transmission-disequilibrium test(TDT) and haplotype-based haplotype relative risk analysis(HHRR) were used to study the correlation between the gene mutation and NSCL/P.

RESULTSHardy-Weinberg equilibrium test results showed that, the offspring and mother genotype in the case group and the control group was not deviated from the Hardy-Weinberg law of genetic equilibrium (P > 0.05). The distribution of genotype CC, CT and TT in offspring had significant difference between the two groups (P < 0.05). In the offspring and mother of case group and the control group, the carriers of the TT genotype compared to CC genotype, the OR are greater than 1 and 95% CI do not contain 1 (offspring OR: 2.692, 95% CI: 1.319-5.495; mother OR: 2.469, 95% CI: 1.136-5.363). The distribution of C allele and T mutation gene were significantly different in the offspring and mother between the two groups (P < 0.05). The TDT test showed: chi2 = 4.507, P < 0.05. The HHRR test showed: P < 0.05.

CONCLUSIONThe single nucleotide polymorphism of MTHFR C677T locus is associated with the development of NSCL/P in Shanxi Province.

Child ; Child, Preschool ; China ; Cleft Lip ; genetics ; Cleft Palate ; genetics ; Female ; Humans ; Infant ; Male ; Methylenetetrahydrofolate Reductase (NADPH2) ; genetics ; Polymorphism, Single Nucleotide

Objective To evaluate the therapeutic effectiveness and toxicity of low-dose cisplatin chemotherapy combined with three-dimensional conformal radiotherapy for mid-advanced esophageal cancer.Methods 89 patients with advanced esophageal cancer either who were inoperable or refused operation were randomized into two groups: 45 cases in the study group and 44 cases in the compared group. The study group

Objective To investigate the accuracy of computer-assisted interpretation(CAI)in the diagnosis of pediatric critical arrhythmia,and to establish Logistic regression model to evaluate the main risk factors of CAI error.Methods 1147 cases of arrhythmia patients were divided into heart disease(HD)and nonheart disease group(NHD).Candidate risk factors were chosen,from which the vailables statistically significant were entered for logistic regression analysis.Results CAI error rate of HD group and NHD group were 24.9%and 11.4%,showing a significant difference(P＜0.05).Age,underlying diseases,the number of CAI diagnosis entries were the risk factors.Conclusion CAI error pattern was different among HD group and the NHD group.Higher awareness for CAI error risk factors and CAI technology improvement are critical for a lower CAI error rate.

Objective To discuss the clinical treatment of unstable dislocation of the sternoclavicular joint with clavicle hook plates. Methods From April 2005 to October 2007, 19 cases of unstable dislo-cation of stemoclavicular joint were treated with open reduction and internal fixation by clavicle hook plates. There were 17 cases of Grade type Ⅲ and 2 cases of Grade type Ⅱ. After open reduction and internal fixation, all patients received arthroplasty and repair of eostoclavicular and sternoclavicular ligaments. Results None had intraoperative injury to nerves and blood vessels. Follow-ups from 6 to 24 (mean, 8) months showed 16 excellent cases, 2 good cases and 1 fair case by Roekwood criteria, the good to excellent rate being 94.7%. The healing duration lasted 3 to 6 (averaging 4) weeks. No infection, sliding or loosening of internal fixation, nonunion or re-dislocation was found postoperatively. Conclusion The clavicle hook plate is an effective and advanced surgical treatment of sternoclavieular joint injuries, because its easy and mini-invasive operation can provide sturdy fixation and lead to good functional recovery.

Forty eight patients with knee joint lipohemarthrosis underwent X-ray, CT, MRI and ultrasonography examinations, the diagnosis was confirmed by knee operation or puncture.The findings of 4 imaging methods were compared.Plain radiographs displayed fracture lines in 39 cases and fat-liquid layer of suprapatellar bursa in 40 cases, it displayed suprapatellar fat-liquid layer in 5 out of 9 occult fracture cases.CT displayed fracture lines in 48 cases and joint lipohemarthrosis in 48 cases; 3D imaging showed fracture lines better.MRI displayed fracture lines in 45 cases and joints lipohemarthrosis in 48 cases, and also showed the intracapsular structure injury.Ultrasonography revealed fracture lines in 15 cases and joint lipohemarthrosis in 48 cases, lipohemarthrosis was displayed in 7 out of 9 occult fracture cases.All 4 diagnostic imaging methods have advantages and disadvantages for diagnosis of lipohemarthrosis, and should be chosen on the basis of clinical needs.

Objective To explore the expression of breast cancer susceptibility gene 1 (BRCA1) in human colorectal cancer and its correlation with the efficacy of platinum-based chemotherapy.Methods Seventy-eight cases of colorectal cancer patients treated with platinum-based chemotherapy after surgery were collected.The surgical specimens of them were taken.Fourteen normal colonic mucosa specimens and 12 non-cancerous tissues of colorectal cancer specimens were obtained.The expression of BRCA1 in tissues was detected by immunohistochemistry and analyzed by x2 test.The colorectal cancer patients were followed up for survival time.Comparison of survival analysis was performed by Kaplan-Meier survival curves and Log-rank test.Results The positive rate of the BRCA1 expression in colorectal cancer tissue (52.6 ％,41/78) was lower than that of para-cancer tissue (11/12,x2 =6.518,P=0.011) and normal colonic mucosa tissue (13/14,x2 =7.949,P=0.005).The poorer the differentiation of colorectal cancer,the lower the positive rate of BRCA1 (x2=14.160,P=0.001).The median disease-free survival time of BRCA1 negative colorectal cancer patients was 51.0 months (95 ％ CI:47.7 to 54.4 months),which was longer than that of BRCA1 positive patients (45.0 months,95 ％CI:36.6 to 53.4 months,x2 =4.367,P=0.032).Conclusions Receiving oxaliplatin based chemotherapy may be a survival benefit for BRCA1 negative colorectal cancer patients.The expression of BRCA1 may be an index for chemotherapy options and prognosis evaluation for colorectal cancer patients after surgery.

Objective To explore the factors related to the outcome of idiopathic facial palsy. Methods 308 patients with idiopathic fa-cial palsy were included. The data of clinic and follow-up were collected from January, 2010 to December, 2014. The data were analyzed by Logistic regression analysis. Results 210 cases (68.2%) were cured (good group) and 98 cases (31.8%) appeared sequelae of different de-grees (poor group). The age, onset of disease, type of disease, damaged section, impaired glucose control, high neutrophil-to-lymphocyte ra-tio (NLR), high blood glucose, high blood triglyceride, time and methods of invention were significant factors related to the outcome (P<0.05). Conclusion Old, servious facial nerve injury, Hunt's palsy, high damaged section, poor glucose control in the patients with diabetes, high NLR, high blood triglyceride, delay and simple invention are independent risk factors for the poor outcome of idiopathic facial palsy.