Objective To explore the effect of melatonin(MT) on connective tissue growth factor(CTGF) and the airway remodeling in asthmatic mice.Methods The mice were randomly divided into 4 groups as control group,asthmatic group,MT treated group and dexamethasone treated group.The mice were sensitized and repeatedly challenged with ovalbumin(OVA) for 2 and 4 weeks.MT treated group were intraperitoneal injected with MT,and dexamethasone treated group were intraperitoneal injected with DXM.The eosinophils around the airway were examined with microscopy after histochemical staining.Masson staining was used to stain collagen fibers and PAS staining was used to stain airway mucus.The following parameters including the perimeter of bronchial basement membrane (Pbm),total bronchial wall area(WAt),inner wall area(WAi),smooth muscle area(WAm),collagen area(Wcol) and mucus area were measured by Leica image analysis software.The protein expression of CTGF was assessed with immunohistochemistry.Results WAt/Pbm,WAi/Pbm,WAm/Pbm,Wcol/Pbm,the mucus area and the expression of CTGF were significantly higher in asthmatic group than those in control group.MT treated group and dexam ethasone treated group significantly alleviated the above-mentioned indicators increased.Conclusion MT inhibits the expression of CTGF and airway remodeling,which has a comparative potency of dexamethasone.

This article was aimed to study the absorption of sinomenine in Caulis Sinome nii by different types of macroporous resin. The transfer rate of sinomenine was used as detection index. The screening was made on the technological process of absorption and purification of sinomenine by macroporous resin. The results showed that the macroporous resin AL-3 was selected with the best absorptive capacity under pH2, while the macroporous resin X-5 was selected with the best absorptive capacity under pH6. It was concluded that the selection of macroporous resin should comprehensively consider the properties of target compounds, and indexes such as aperture, specific surface area, and polarity of macroporous resin.

Objective:To investigate the correlation between circadian blood pressure pattern and heart rate variability and stroke severity and outcome in patients with acute ischemic stroke (AIS).Methods:Patients with first-ever AIS admitted to the Affiliated Qingdao Central Hospital of Qingdao University from January 2015 to January 2021 were retrospectively included. Ambulatory blood pressure monitoring (ABPM) and ambulatory electrocardiogram (AECG) were performed after admission. The severity of stroke was assessed according to the National Institutes of Health Stroke Scale. ≤8 were defined as minor stroke, and >8 were defined as moderate to severe stroke. The modified Rankin Scale was used to evaluate the clinical outcome at 3 months after onset. ≤ 2 were defined as good outcomes, and >2 were defined as poor outcomes. Multivariate logistic regression analysis was used to determine the independent influencing factors of stroke severity and outcome. Results:A total of 516 patients with AIS were enrolled, including 328 male (63.57%), aged 59.62±6.67 years old. Among them, 266 patients (51.55%) were in the minor stroke group and 250 (48.45%) were in the moderate to severe stroke group. There were 463 patients (89.73%) were in the good outcome group and 53 (10.27%) were in the poor outcome group. Multivariate logistic regression analysis showed that hypertension (odds ratio [ OR] 5.021, 95% confidence interval [ CI] 2.635-10.923; P<0.001), atrial fibrillation ( OR 3.896, 95% CI 2.574-8.521; P<0.001), circadian blood pressure pattern (non-dipper type: OR 2.436, 95% CI 1.031-4.749, P<0.001; reverse dipper type: OR 2.654, 95% CI 1.642-5.268, P<0.001), SDNN ( OR 0.298, 95% CI 0.114-0.730; P=0.002), SDANN ( OR 0.325, 95% CI 0.200-0.679; P=0.009), rMSSD ( OR 0.437, 95% CI 0.255-0.876; P=0.016) and pNN50 ( OR 0.369, 95% CI 0.291-0.767; P=0.013) were the independent influencing factors of stroke severity. Hypertension ( OR 4.857, 95% CI 1.957-8.552; P<0.001), baseline NIHSS score ( OR 2.189, 95% CI 1.597-3.315; P<0.001), stroke severity ( OR 3.853, 95% CI 2.316-5.958; P<0.001), circadian blood pressure pattern (non-dipper type: OR 2.997, 95% CI 1.128-5.430, P<0.001; reverse dipper type: OR 3.703, 95% CI 1.478-5.902; P<0.001), SDNN ( OR0.369, 95% CI 0.215-0.779; P=0.015), SDANN ( OR 0.372, 95% CI 0.198-0.862; P=0.018), rMSSD ( OR 0.455, 95% CI 0.314-0.896; P=0.026) and pNN50 ( OR 0.448, 95% CI 0.307-0.825; P=0.021) were the independent influencing factors of poor outcomes. Conclusion:The non-dipper and reverse dipper circadian blood pressure patterns and lower heart rate variability are independently associated with stroke severity and poor outcomes in patients with AIS.