1.Effect of Aima recipe on the protein and mRNA expression of TNF? and ICAM-1 in the bronchus of rats with chronic bronchitis
Guanghua TANG ; Qifu HUANG ; Liangdu JIANG
Chinese Journal of Pathophysiology 2000;0(07):-
AIM: To study the effect of Aima recipe (AM), a traditional Chinese medicine, on the protein and mRNA expression of TNF? and ICAM-1 in the bronchus of rats with chronic bronchitis(CB). METHODS: 15 male Wistar rats were randomly divided into three groups: control group, chronic bronchitis (CB) group, CB plus AM group. The protein and mRNA expression of TNF? and ICAM-1 were assayed by immunohistochemistry and in situ hybridization methods. RESULTS: TNF?, ICAM-1 protein and mRNA were more strongly expressed in the area of bronchial epithelium in the CB group compared with control group ( P
2.Design and realization of expendable network request system based on"No 1. Military"HIS
Fei XIAO ; Zhengdong HUANG ; Guanghua WANG
Chinese Medical Equipment Journal 1989;0(04):-
As a chief part of hospital information system, the expendable management system plays an important role in expendable normalization, scientific management, efficiency improvement, labor lessening and mistake reduction. This paper mainly introduces the design of expendable network request system based on "No.1 Military" HIS and its main function as well as characteristics.
3.Scheme of hospital intranet security
Guanghua WANG ; Xueqing GUO ; Zhengdong HUANG
Chinese Medical Equipment Journal 1993;0(05):-
By the presentation of current intranet development and the analysis of misunderstandings and challenges in intranet security,associated with the security product and the hospital practice,a scheme for establishing a controllable and creditable intranet is put forward.
4.Application of Telemedicine Consultation Vehicle in Field Medical Training
Xueqing GUO ; Guanghua WANG ; Zhengdong HUANG
Chinese Medical Equipment Journal 1993;0(05):-
The working principle and points for attention of telemedicine consultation vehicles are expatiated,and solutions for regular malfunction are given.
5.The influence of individual behavior intervention on emotion and quality of life of elderly females with stress urinary incontinence
Huifang SUN ; Guanghua LAI ; Yasheng HUANG
Chinese Journal of Primary Medicine and Pharmacy 2016;23(2):190-194,195
Objective To observe the influence of individual behavior intervention on emotion and quality of life of elderly females with stress urinary incontinence and provide guidance for health intervention.Methods 124 elderly females with stress urinary incontinence were selected.Individual behavior intervention was used,and after 1,3,6 months,the effect was evaluated by anxiety,depression,ICIQ -FLUTS,UDI -7,and SCL -90.Results After the individual behavior intervention,anxiety and depression scores became lower[anxiety scores:before intervention (59.16 ±6.12)points,1month (52.68 ±5.62)points,3months (48.28 ±5.32)points,6months (39.86 ± 4.27)points,there were statistically significant differences between the two groups(F =298.69,P =0.000).Depres-sion scores:before intervention (59.16 ±6.12)points,1 month (52.68 ±5.62)points,3 months (48.28 ± 5.32)points,6 months (39.86 ±4.27)points,there were statistically significant differences between the two groups (F =252.10,P =0.000)].ICIQ -FLUTS and UDI -7 scores became less[ICIQ -FLUTS scores:before interven-tion (31.79 ±3.74)points,1 month (29.62 ±3.18)points,3 months (27.54 ±2.67)points,6 months (23.32 ± 2.16)points,there were statistically significant differences between the two groups(F =161.15,P =0.000).UDI -7 scores:before intervention (19.29 ±1.76)points,1 month (16.09 ±1.48)points,3 months (14.32 ±1.37)points, 6 months (11.57 ±1.26)points,there were statistically significant differences between the two groups(F =531.23, P =0.000)].There were significant differences in somatization,obsession,sensitivity of interpersonal relationship, depression,anxiety,hostility,fear,crankiness and psychotic diseases(somatization scores:F =18.933,P =0.000;obsession:F =5.153,P =0.000;sensitivity of interpersonal relationship:F =3.314,P =0.020;depression:F =9.306,P =0.000;anxiety:F =4.902,P =0.002;hostility:F =6.852,P =0.000;fear:F =7.782,P =0.000;cranki-ness:F =11.917,P =0.000;psychotic diseases:F =8.560,P =0.000).Conclusion After the individual behavior intervention,anxiety,depression,ICIQ -FLUTS,UDI -7,and scl -90 scores become lower.Individual behavior inter-vention has a positive effect on emotional states and quality of life,which is worthy of clinical promotion.
6.Safe realization of "No.1 Military" database upgrade based on standby server
Guanghua WANG ; Fei XIAO ; Zhengdong HUANG
Chinese Medical Equipment Journal 2004;0(08):-
This paper introduces the safe realization of ″No.1 Military″ database upgrade from ORACLE7.33 to ORACLE8I by import/export tools based on standby server.The establishment of standby server and the process of No.1 Military database upgrade are introduced detailedly in this paper.The database upgrade of other application system adopting ORACLE database system can refer to this paper.
7.ANALYSIS AND CHARACTERIZATION OF CAROTENOIDS IN GINGKO SEED
Wen HUANG ; Bijun XIE ; Yi WANG ; Rui LUO ; Guanghua PENG
Acta Nutrimenta Sinica 1956;0(03):-
Objective: To analyze and identify the carotenoid in gingko seed.Methods: The chemical composition of the pigment from gingko seed was identified as carotenoid by UV absorption spectrum. Its content was determined by spectrophotometric method and its composition was analyzed and identified by thin-layer chromatography and HPLC method.Results: Gingko contained carotenoids, roughly about 489 ?g/100 g in which lutein, ?-carotene and ?-carotene amounted to 69.20%,15.80% and 7.45% respectively. Conclusion: Gingko contained large quantity of carotenoids, mainly lutein, and next in order ?-carotene and ?-carotene.
8.Biocompatibility of acellular tracheae as scaffold for artificial salivary gland organoid
Guilin HUANG ; Longjiang LI ; Guanghua PAN ; Chunyu WANG ; Nini ZHANG
Chinese Journal of Tissue Engineering Research 2008;12(23):4587-4590
BACKGROUND: An important initial step in developing a tissue engineering artificial salivary gland organoid is to find an ideal scaffold. To find other new biomaterials should to be further studied.OBJECTIVE: To obtain an acellular matrix from tracheae of rabbits and SD rats, and to investigate its biocompatibility as a primary step toward developing a tissue engineering artificial salivary gland organoid.DESIGN, TIME AND SETTING: A randomized controlled animal study, which was performed at the Key Laboratory of Transplantation Engineering and Immunology of Ministry of Health, West China Hospital of Sichuan University from February 2003 to May 2005.MATERIALS: A modified detergent and enzyme link extraction procedure was performed to remove cells from SD rats and rabbits tracheae. The histology, topography of inner-surface and biocompatibility were studied on both acellular tracheae.METHODS: Eighteen SD rats were randomly divided into 2 groups. One group was planted acellular trachea of SD rats. Other group consisted of acellular trachea of rabbits. On the third generations, submandibular gland cells were inoculated on two acellular tracheae and cultured on PGA membrane; while, cells in the control group were inoculated on 12-well culture plate, and cell/scaffold complex was cultured at the same time.MAIN OUTCOME MEASURES: The structure and topography of inner-surface of the acellular tracheae matrixes were observed both by light and scanning electron microscopy. The inflammatory response of the tissue around acellular tracheae implanted under the skin of cheek was evaluated at 1, 4, 12 weeks. The numbers of cells grown on the acellular tracheae and PGA film were counted at 1, 2, 3, 4, 5, 6, 7 days. At 1, 3, 5, 7 days, the mean values of metabolic activity test and the amylase activities of supernatants of the cells/scaffold complexes were examined.RESULTS: The cells were completely removed from both tracheae. No evident inflammatory response was found in tissues around two kinds of acellular tracheae implanted under the skin of cheek. The number of submandibular gland cells (SSG) grown on the two kinds of naturally derived biomaterials was much more than grown on PGA (P<0.05). The mean values of metabolic activity test and the amylase activities of supernatants containing cell/acellular matrixes were much higher than that of cell/PGA (P<0.05).CONCLUSION: The acelhilar tracheae matrixes made by our laboratory can be used as scaffold in the study of tissue engineering artificial salivary gland organoid.
9.Effect of gamma knife surgery on treatment of craniopharyngiomas
Wei WU ; Guanghua LUO ; Keming YING ; Bensheng HUANG ; Tongfang YUANG
Chinese Journal of Primary Medicine and Pharmacy 2009;16(8):1403-1404
Objective To evaluate the adaption,effectiveness,dose,complication of the treatment for cranio pharygiomas with gamma knife surgery.Methods Gamma knife surgery was performed in 41 patients.Patients with mixed solid and cystic tumors were treated with stereotaxic aspiration while six cases were treated with divergence surgery prior to gamma knife therapy.The central dose ranges from 17.1 ~40 Gy(29.6Gy in average).The patients were treated at 30%~50% equal dose curve with 6 ~14Gy of tumormargin dose(9.5Gy in average).The exposure dose of the optic nerve and optic tract is less than 10Gy.Results Of twenty-nine patients who were followed up from 6 to 100 months,sixteen had disappeared or decreased tumor,six had unchanged,two was performed craniotomy one year or three years after gamma knife surgery,and the remaining five were dead one year to three years.The tumor control rate was 75.9%(22/29).Conclusion The treatment of stereotaxic radiation with single and high dose is sensitive to most of the solid craniopharyngioma,and the treatment of stereotaxic resection combined with gamma knife surgery may be feasible for the recurrent mixed solid and cystic craniopharyngioma.
10.Design and Actualization of Hospital Architecture Intelligentized System
Zhengdong HUANG ; Xueqing GUO ; Guanghua WANG ; Fei XIAO
Chinese Medical Equipment Journal 1989;0(03):-
Hospital building intelligentized system is an important part of digital hospital. Innovative theory in technique integration and service idea of "taking sufferers as center" is incarnated by its design and its development direction of current hospital building. The concept and design principle of intelligentized system in hospital architecture construction are discussed; the composing and function of intelligentized system are also expounded; characteristic and application effect of the system in hospital architecture are analyzed.