This paper discusses the definition , classification, selection, monitoring and evaluation of animal biosafety isolation device .Evaluation order of animal biosafety isolation device follows animal survival needs -biosafety needs-animal welfare requirements .

In 2013 Shanghai took the lead to carry out the specialist standardized training which is common in the international medical education. This paper first gives a picture of the general condition of the standardized training on specialists of obstetrics and gynecology in Shanghai. Then from the perspectives of strategic deployment, department management, clinical skill training and the training of examiners, the paper explores the assessment model in which both the assessment of train-ing process and the scores of the final examination are considered whereas the process assessment is given more weight. This paper is aimed to provide experience and suggestions for the further advance of the specialists standardized training in the field of obstetrics and gynecology.

To analyze the potential limitations of hospital-level research projects management by summarizing its performance status from 2008 to 2011,including but not limited to lack of time for conducting research,insufficient financial funds,inadequate funding application,inadequate supervision.Thus,since 2014,our hospital has adopted some new measurements for projects management,including full-time research,hierarchical management,full mobilization,the establishment of reward and punishment measures,which significantly improved the quality of hospital-level research projects,and the rate of longitudinal follow-up project,research enthusiasm of medical staff,and sustainable development of hospital science and technology.

Objective To investigate the clinical significance of Wilm tumor gene (WT1) expression in breast cancer. Methods Real-time quantitative reverse transcriptase polymerase chain reaction (RQ-RT-PCR) method was established for detecting WT1 and GAPDH mRNA expression levels in 110 cases of various breast tumor and the corresponding adjacent normal breast tissue. Normalized WT1 expression level (WT1_N)was determined as a ratio between WT1 and GAPDH for each case. The tumor tissue WT1_N over the normaltissue WT1_N of the same case was calculated as T/N_(WT1) ratio, and T/N_(WT1) value was analyzed with the clinicalpathological parameters. Results The WT1_N expression levels of the 102 breast cancer tissues were significantly higher than those of the adjacent normal breast tissues, with the median WT1_N of 2.38 (ranged from 0.12 to 112.3) and 0.81 (ranged from 0.03 to 11.65) for each (P <0.01), but there were no statistical differences between the WT1_N of 8 benign breast tumors and the nearing normal tissues, with the median WT1_N of 0.46 (ranged from 0.16 to 5.04) and 0.53 (ranged from 0.14 to 4.94) for each. Furthermore the WT1_Nas well as the T/N_(WT1) ratio of the malignant breast cancer tissues were significantly higher than those of the benign tumor tissues, with the median T/N_(WT1) value of 2.54 (ranged from 0.28 to 172.88) and 1.17 (ranged from 0.09 to 2.63) for each. Non-parameter correlation analysis showed that the T/N_(WT1) in breast cancers were of no relevance to lymph node metastasis, clinical-pathological types, estrogen receptor and progestone receptor status, but positively correlated with the expression level of IL-8 gene which calculated with T/N IL-8(r =0.723, P <0.01). Conclusion The WT1 gene is highly expressed in breast cancer which suggests that WT1 level assessed by RQ-RT-PCR could be a novel marker of disease progression and poor prognosis.

Objective To investigate the curative effect of laparoscopic uterine abdominal wall suspension on the treatment of patients with moderate and severe pelvic organ prolapse.Methods Totally 23 cases with moderate and severe pelvic organ prolapse were treated with laparoscopic uterine abdominal wall suspension.According to the internationally recognized pelvic organ prolapse (POP-Q),the values of POP-Q before and after treatment were compared.The quality of life of PFDI-20,POPDI-6,CARDI-8,UDI-6 and PISQ-31 scores (SAS) and the sleep status self-rating scale (SRSS) were used to compare the mental status of the patients before and after the operation (P ＜ 0.01).Results After treatment (except vaginal length),scores of POP-Q were significantly better than those before treatment (P ＜ 0.01).After six months of follow-up,PFDI-20,POPDI-6,CARDI-8,UDI-6 score significantly decreased,while PISQ-31 scores increased significantly (P ＜ 0.01).The incidence rate of postoperative complications was significantly lower than that before operation (P ＜0.01).Postoperative SAS score and SRSS score significantly decreased (P ＜ 0.01).Conclusion For patients with moderate and severe pelvic organ prolapse,the laparoscopic uterine abdominal wall suspension can effectively ensure the integrity of the pelvic floor structure and improve the quality of life of patients.

Objective To investigate the curative effect of laparoscopic uterine abdominal wall suspension on the treatment of patients with moderate and severe pelvic organ prolapse.Methods Totally 23 cases with moderate and severe pelvic organ prolapse were treated with laparoscopic uterine abdominal wall suspension.According to the internationally recognized pelvic organ prolapse (POP-Q),the values of POP-Q before and after treatment were compared.The quality of life of PFDI-20,POPDI-6,CARDI-8,UDI-6 and PISQ-31 scores (SAS) and the sleep status self-rating scale (SRSS) were used to compare the mental status of the patients before and after the operation (P ＜ 0.01).Results After treatment (except vaginal length),scores of POP-Q were significantly better than those before treatment (P ＜ 0.01).After six months of follow-up,PFDI-20,POPDI-6,CARDI-8,UDI-6 score significantly decreased,while PISQ-31 scores increased significantly (P ＜ 0.01).The incidence rate of postoperative complications was significantly lower than that before operation (P ＜0.01).Postoperative SAS score and SRSS score significantly decreased (P ＜ 0.01).Conclusion For patients with moderate and severe pelvic organ prolapse,the laparoscopic uterine abdominal wall suspension can effectively ensure the integrity of the pelvic floor structure and improve the quality of life of patients.

OBJECTIVETo study localization and expression of CSF-1 receptor protein, in order to discover the CSF-1 and IL-1alpha effects on CSF-1 receptor mRNA levels and to determine if the autocrine effect is inhibited through the CSF-1 receptor.

METHODSImmunolocalization of CSF-1 receptor in the cultured dental follicle cells and in mandibles of the post-natal rats from day 1 to 11 were performed. The effects of different concentrations of CSF-1, IL-1alpha on CSF-1 receptor gene expression were detected by means of RT-PCR.

RESULTSCultured dental follicle cells were immunostained for the CSF-1 receptor. In vivo, immunostaining showed that the CSF-1 receptor was present in the dental follicle of the first mandibular molar at early post-natally and was either absent or greatly reduced by day 11 post-natally. High concentrations of cvCSF-1 reduced the gene expression of the CSF-1 receptor. IL-1alpha had no effects on CSF-1 receptor mRNA levels.

CONCLUSIONSThe expression of CSF-1 receptor reaches a peak early post-natally in the dental follicle of the first mandibular molar of the rat and then subsequently declines. High concentrations of CSF-1 inhibits the expression of CSF-1 receptor, IL-1alpha has no effect on the expression of CSF-1 receptor mRNA.

Animals ; Cells, Cultured ; Dental Sac ; chemistry ; cytology ; Immunohistochemistry ; Interleukin-1 ; pharmacology ; Macrophage Colony-Stimulating Factor ; pharmacology ; RNA, Messenger ; analysis ; Rats ; Rats, Sprague-Dawley ; Receptor, Macrophage Colony-Stimulating Factor ; analysis ; genetics

We propose an algorithm for segmentation of the overlapped leukocyte in the microscopic cell image. The histogram of the saturation channel in the cell image is smoothed to obtain the meaningful global valley point by the fingerprint smoothing method, and then the nucleus can be segmented. A circular region, containing the entire regions of the leukocyte, is marked off according to the equivalent sectional radius of the nucleus. Then, the edge of the overlapped leukocyte is represented by polar coordinates. The overlapped region by the change of the polar angle of the edge pixels is determined, and the closed edge of the leukocyte integrating the gradient information of the overlapped region is reconstructed. Finally, the leukocyte is exactly extracted. The experimental results show that our method has good performance in terms of recall ratio, precision ratio and pixel error ratio.
Algorithms ; Cell Adhesion ; Humans ; Image Enhancement ; instrumentation ; methods ; Image Processing, Computer-Assisted ; instrumentation ; methods ; Leukocytes ; cytology ; Microscopy

OBJECTIVETo compare the therapeutic effects of triple puncture at Tianzong (SI 11) and routine acupuncture on obstinate tennis elbow.

METHODSSixty-two cases were randomly divided into 2 groups. The treatment group of 32 cases were treated with triple puncture at Tianzong (SI 11) combined with local points of the elbow, and the control group of 30 cases were treated with local points of the elbow. After one therapeutic course, changes of the symptoms and signs between the two groups were compared.

RESULTSThe cured rate of 71.900 in the treatment group was significantly better than 43.3% in the control group (P < 0.05).

CONCLUSIONTriple puncture combined with routine acupoint selection therapy has a better therapeutic effect on obstinate tennis elbow than the routine acopoint selection therapy.

Activities of Daily Living ; Acupuncture Points ; Acupuncture Therapy ; methods ; Adult ; Aged ; Female ; Humans ; Male ; Middle Aged ; Tennis Elbow ; therapy

Objective: To investigate the antibacterial activity to Streptococcus mutans of a nisin-containing single-bond universal adhesive. Methods: Nisin was mixed into the bonding agent to produce concentrations ranging from 0.01 g/mL to 0.05 g/mL for the experiments, and adhesive without nisin was used as the control. Dentin-resin specimens were prepared for the microtensile strength test to evaluate changes in the bonding strength. The proper concentrations were selected for more tests. ① An agar diffusion test was applied with filter paper to detect the release of nisin, and adhesive without nisin was used as the negative control, 0.01 g/mL Nisin aqueous solution was used as the positive control. ② Solidification; resin adhesive specimens were prepared for the assessment of direct contact inhibition activity. ③ Confocal laser scanning microscopy was used to examine the effect of the adhesive on the biological film activity and the ability of Streptococcus mutans to produce extracellular polysaccharides. Results : Nisin did not significantly reduce the bond strength of the modified adhesive at 0.01-0.03 g/mL (P < 0.05); these concentrations were selected for the subsequent antibiosis experiment. Rings could not be observed in the agar diffusion test, except for in the group of adhesive modified with 0.01-0.03 g/mL nisin. Resin adhesive with 0.01-0.03 g/mL nisin could significantly inhibit the proliferation of Streptococcus mutans on the surface of the specimens. The confocal laser scanning microscopy results indicate that only the adhesive resin modified with nisin could reduce the bacteria in the biofilm and the production of extracellular polysaccharides. Conclusion Single-bond universal adhesive with 0.01-0.03 g/mL nisin can inhibit the growth of Streptococcus mutans and its biofilms on the bonding interface, as well as decrease the production of extracellular polysaccharides, and thus has the potential to decrease the occurrence of secondary caries.