Objective To knockout and identify the Antigen 43 (Ag43) in the Escherichia Coli JM109 .Methods Mutation group Ⅱ introns RNA protein complexes (RNP) gene sequence was obtained by Sigma Company′s TargeTron Gene Knockout Sys‐tem and Ag43 gene specific designed PCR primers amplification ,then ,to acquired Ag43 specific recombinant RNP plasmid pACD4K‐Ag4 ,this gene sequence was inserted into the plasmid pACD4K‐C of RNA′s expression .Finally ,pEGFP‐Ag43 was trans‐formed into JM109 and inserted the group Ⅱ intron into the Ag43′s locus by IPTG inducing expression .Results The best insertion locus was between 1 812 and 1 913 .Through the agarose electrophoresis gel ,the RNP gene sequence was consistent with the expec‐ted value (350 bp) .The pEGFP‐Ag43 vector was correctly constructed which was proofed by endonuclease Nhe Ⅰ and Hind ⅡI di‐gestion as predicted products (3 646 and 4 029 bp;7 000 and 550 bp ,respectively ) .The PCR and gene sequence results indicated that the group Ⅱ intron was inserted into the locus between 1 812 and 1 913 in the Ag43 gene .Conclusion Successful knockout of the Ag43 in Escherichia Coli JM109 found basis to further study the Ag43′s function and regard the coli as host bacteria of Ag43 chimeric protein recombinant .

Objective To explore the effect of small dose of dexmedetomidine on nausea and vomiting in the patients with cervical cancer minimally invasive surgery .Methods One hundred and twenty-two cases of cervical cancer minimally invasive sur-gery in the hospital from June 2014 to June 2016 were selected as the research subjects and divided into the control group and obser-vation group according to the random number table method ,61 cases in each group .The observation was intravenously injected by 0 .1μg/k dexmedetomidine for 1 min before anesthesia induction ,while the control group was given the same volume of normal sa-line .The perioperative indexes ,nausea and vomiting grade and adverse reactions occurrwence were comparfed between the two groups .The anesthesia time ,operative time ,eye opening time ,autonomous respiratory recovery time and tracheal tube extubation time had no statistical differences between the two groups were compared .Results The intraoperative HR minimum value in the observation group was lower than that in the control group ,the bradycardia occurrence rate was higher than that in the control group ,the difference was statistically significant (P<0 .05) .The constituent ratio of PONV grade Ⅱat T2 time point in the obser-vation group was significantly lower than that in the control group ,and the occurrence rate of nausea and vomiting at T 4(postopera-tive 24 h) in the observation group was significantly lower than that in the control group ,the difference was statistically significant (P<0 .05) .The occurrence rate of agitation in the control group was higher than that in the observation group ,and the difference was statistically significant (P<0 .05) .Conclusion Small dose of dexmedetomidine can significantly alleviate postoperative nausea and vomiting in the patients with cervical cancer minimally invasive surgery ,moreover can decrease the agitation occurrence rate without affecting the effect of anesthesia ,but is prone to develop bradycardia .