Objective To investigate the optimal initial concentration of microRNA22 agomir in epilepsy model induced by lithium chloride-pilocarpine after single injection of lateral ventricle.Methods 36 rats with acute temporal lobe epilepsy were randomly divided into 6 groups:the control group and the other five groups were the experimental group.All epilepsy rats were selected for right lateral ventricle injection.The control group was given negative control reagent,while the experimental group were given 0.1 mmol/L,2.5 mmol/L,5 mmol/L,10 mmol/L,20 mmol/L different concentrations of miRNA22agomir reagent.6 rats in each group were randomly selected for acute phase experiment after 3 days of administration.The expression of P2X7 in hippocampus of epilepsy rats was determined by Western blot and quantitative real-time polymerase chain reaction (qRT-PCR).Results Compared with control group,the protein and mRNA expression of P2X7 reduced in all of the model group.The protein and mRNA expression level of P2X7 protein in hippocampus of rats injected with 2.5 mmol/L,5 mmol/L and 10 mmol/L in each experimental group were significantly lower than that in the other two groups (P ＜ 0.05).Moreover,the protein and mRNA expression level of P2X7 were the lowest at 2.5 mmol/L injection and 10 mmol/L,and there was no significant difference between the two groups (P ＞ 0.05).Conclusions The optimal onset concentration for unilateral lateral ventricle injection miRNA22 agomir treatment of temporal lobe epilepsy is 2.5 mmol/L.

Objective:To study the effect of micro-course teaching combined with teacher standardized patient combined with objective structured clinical examination (TSP/OSCE) on clinical practice teaching of pediatric nursing.Methods:Forty nurses who performed clinical practice in Pediatrics Department from February 2019 to July 2019 were selected as the control group, and traditional teaching was adopted in the group. In addition, 42 nurses who performed clinical practice in Pediatrics Department from August 2019 to January 2020 were selected as the research group, and the micro-course teaching combined with TSP/OSCE mode teaching was adopted in the group. Two groups of nurses' theoretical and practical skills assessment results were analyzed, and a questionnaire survey was conducted to assess nurses' satisfaction with the teaching of pediatric nursing clinical practice training mode. SPSS 22.0 was used for t test and chi-square test. Results:The research group nurses' theoretical and practical skills assessment scores were better than those of the control group, and the difference was statistically significant ( P=0.000).The nurses in the research group were more satisfied than those in the control group with such eight aspects as improving practical skills, emergency response capabilities, humanistic care and service capabilities, communication skills, and stimulating their interest in nursing learning. There were statistically significant differences ( P<0.05). Conclusion:The use of micro-teaching combined with TSP/OSCE mode in the clinical practice teaching of pediatric nursing can improve nurses' clinical practice ability and increase nurses' satisfaction in pediatric training.

OBJECTIVE To explore the relationship between laryngeal morphology and clinical function after partial laryngectomy for glottic laryngeal cancer,as assessed by CT.METHODS This study included 90 patients with glottic laryngeal cancer who underwent partial laryngectomy between March 2020 and March 2023(observation group).Postoperative follow-up included CT scans,measuring glottal area(GA),glottal width(GW),glottal depth(GD),subglottic area(SGA),and hyoid-cricoid distance(HCD).Postoperative respiratory,phonation,and swallowing functions were recorded.The study compared CT morphological parameters and voice acoustic parameters between the observation group and 50 healthy volunteers(control group)and analyzed the correlation of laryngeal CT morphological parameters with decannulation time,swallowing function grading,and voice acoustic parameters.RESULTS All 90 patients were decannulated at follow-up,with decannulation times ranging from 7 to 22(14.35±3.67)days.Laryngoscopy showed that 58 patients had complete glottal closure while phonating/i:/,whereas 32 had incomplete closure.At follow-up,all patients were able to eat orally.Swallowing function assessment results were:grade 0 in 62 cases(68.89％),grade 1 in 23 cases(25.56％),and grade 2 in 5 cases(5.55％).Postoperative laryngeal CT in the observation group revealed varying degrees of structural deficiencies in the vocal cords,laryngeal ventricle,and ventricular band.Glottal morphology appeared as'V','U'shaped,or irregularly abnormal,with some patients showing slight enlargement or deviation of the glottal slit.CT morphological parameters GA,GW,GD,SGA,HCD in the observation group were all smaller than those in the control group(P＜0.05).Those in the observation group with complete glottal closure during/i:/phonation had larger GA,GW,GD,SGA,HCD than those with incomplete closure(P＜0.05).Voice acoustic analysis revealed that postoperative F0,MPT were lower in the observation group compared to the control group(P＜0.05),while Jitter and Shimmer were higher(P＜0.05).Spearman correlation analysis showed a negative correlation between postoperative laryngeal CT morphological parameters GA,GW,GD,SGA,HCD and swallowing function grading in the observation group(P＜0.05).Pearson correlation analysis showed a positive correlation of these parameters with F0,MPT(P＜0.05),and a negative correlation with decannulation time,Jitter and Shimmer(P＜0.05).CONCLUSION Laryngeal CT morphological parameters GA,GW,GD,SGA,HCD are closely related to respiratory,phonation,and swallowing functions in patients after vertical partial laryngectomy for glottic laryngeal cancer.These parameters can be helpful in guiding clinical treatment and rehabilitation training.

Objective:To explore the effects of family-centered conductive education on the muscle strength in children with cerebral palsy and the stress of caregivers.Methods:Totally 92 children with cerebral palsy and 92 their main caregivers admitted into the Affiliated Hospital of Jining Medical University from October 2018 to December 2019 were selected and divided into a control group and a conductive education group according to the random number table, with 46 children with cerebral palsy and their 46 main caregivers in each group. Patients in the control group received routine care, while patients in the conductive education group underwent family-centered conductive education on the basis of routine care. The Chinese version of the Modified Ashworth Scale (MAS) , Caregiver Strain Index (CSI) , and the Chinese version of Family Management Measure (FaMM) were used to evaluate the muscle strength, caregiver pressure, and disease management ability of the two groups before and after intervention. Therefore, there were actually 43 cases in the control group and 42 cases in the conductive education group.Results:After intervention, the MAS scores of the two groups were lower than those at admission, and the score was lower in the conductive education group than in the control group, with statistically significant differences ( P<0.05) ; the CSI score of caregivers in the conductive education group was lower than that in the control group, and the difference was statistically significant ( P< 0.05) , while the FaMM score was higher than that in the control group, with statistically significant difference ( P<0.05) . Conclusions:Family-centered conductive education can effectively reduce the muscle strength of children with cerebral palsy, alleviate the pressure of their caregivers, and improve the caregivers' disease management ability.

Research teams work together to solve the technical bottleneck in the Panax notoginseng industry.These research directions mainly focus on breeding of new variety,alleviating the continuous cropping obstacles,building standard system and so on.We used methods such as biotechnology,information technology and so on,to realize digitization,network and intellectualization in the process of selection of producing areas,field management,harvest storage,solved key issues of the precise selection of producing areas,variety breeding,soil improvement,fertilization management,and realized the precision and optimization of ginseng cultivation.We built the composite technology system of pollution-free notoginseng.This technology system mainly contained the breeding of new cultivar,the composite technology of soil improvement,the cultivation system of pollution-free notoginseng and quality traceability system.A new disease-resistant cultivar of notoginseng was bred,which offered feasibility for the notoginseng continuous cropping.We first presented composite improvement technology of notoginseng continuous cropping soil.This improvement technology has been used in the field.This technology system of soil restoration and improvement guaranteed notoginseng continuous cropping.A first standard system of pollution-free notoginseng was issued.It established standard of high-quality notoginseng materials.Finally,quality traceability system of notoginseng was finished,which safeguarded the controllable quality.This composite technology system offers references for the cultivation of other Chinese herbal medicines.

Objective:To explore the characteristics of endothelial function in children with incomplete Kawasaki disease (IKD) of different ages and its relationship with coronary artery lesion (CAL).Methods:A total of 200 children with IKD admitted to the Affiliated Hospital of Jining Medical University from February 2020 to May 2022 were selected as the IKD group, and another 200 healthy children who underwent physical examinations during the same period were selected as the control group. According to the age of children with IKD, infants ( n=78) were classified as those under 1 year old, infants ( n=62) were classified as those between 1-3 years old, and preschool children ( n=60) were classified as those over 3-6 years old. The endothelial function characteristics of IKD children in different age groups and normal control group children were analyzed and compared. In addition, IKD patients were divided into CAL group ( n=110) and nCAL group ( n=90) based on whether CAL was merged. The age, carotid intima-media thickness (IMT), flow-mediated-dilation (FMD), carotid stiffness index (SI), nitroglycerin-mediated dilation (NMD), tumor necrosis factor α (TNF-α), C-reactive protein (CRP), brachial artery endothelial dependent dilation function (EDD) and other clinical data were compared between the two groups. Cox proportional hazards model was used for univariate and multivariate analysis to identify the risk factors for CAL in IKD patients. A column chart prediction model was constructed and evaluated. Results:There were statistically significant differences in FMD, NMD, SI, IMT, reactive congestion index (RHI), and EDD between IKD patients and normal control group children (all P<0.05). There were statistically significant differences in FMD, NMD, SI, IMT, RHI, and EDD among three groups of IKD children of different ages (all P<0.05). There were statistically significant differences in the proportion of children aged ≤3 years, TNF-α, CRP, SI, IMT, EDD, FMD, and NMD between the nCAL and CAL groups (all P<0.05). Age ≤3 years, elevated levels of TNF-α, CRP, SI, IMT, and EDD, as well as decreased levels of FMD and NMD, were independent risk factors for CAL in children with IKD (all P<0.05). The column chart prediction model had high discrimination, and the area under the curve for predicting CAL occurrence in children with IKD was 0.868 and 0.830, respectively. Conclusions:There are significant differences in endothelial function among children with IKD of different ages, and endothelial function is closely related to the occurrence of CAL. When endothelial function is abnormal, CAL is more likely to occur.