The teaching goal of the eight-year program of clinical medicine emphasizes on high standard,starting point and requirement. In order to improve the teaching quality of obstetrics and gynecology,the teaching faculties constantly introduced the most updated concepts from overseas into their teaching experience ,including the concepts of practical translational medicine ,using of English multimedia teaching materials, real case study and the communication skills between doctors and patients. It showed that efforts put in the reformation of teaching material improved the learning interests of students and the professional skills of teachers.

Objective: To explore the value of diffusion-weighted imaging (DWI) combined with conventional magnetic resonance imaging (MRI) in diagnostic staging and the decision of treatment method in patients with cervical cancer. Methods: The data of DWI MRI from 42 patients pathologically confirmed with cervical cancer were retrospectively analyzed. The apparent diffusion coeffcient (ADC) and exponential ADC (eADC) values were measured. The ADC and eADC values were compared according to different pathologic types and stages of cervical cancer, and between the cervical cancer and normal cervix. The diagnostic accuracy of DWI combined with conventional MRI for pathologic staging was examined. The ADC and eADC values were compared between radiochemotherapy group and the surgery group. Results: The cervical cancer demonstrated high signal intensity on DWI, whereas the normal cervix showed no obviously abnormal signal intensity. The mean ADC value of 42 patients with cervical cancer was lower than that of normal cervix (P = 0.000); the mean ADC value of cervical squamous-cell cancer was lower than that of adenocarcinoma (P = 0.036); the mean ADC value of radiochemotherapy group before treatment was lower than that of surgery group (P = 0.000), whereas the eADC value was higher than that of surgery group (P = 0.000). There were no significant differences in mean ADC and eADC values among different pathologic stages of cervival cancer (all P values < 0.05). The diagnostic accuracy of conventional MRI and DWI combined with conventional MRI for pathologic staging were 92.9% (39/42) and 95.2% (40/42), respectively (Kappa = 0.903 5 and 0.935 8, respectively; P < 0.01). Conclusion: DWI combined with MRI exerts a high value in diagnostic staging of cervical cancer. The ADC value can be used as reference in selection of therapeutic methods for stage II B cervical cancer.

This study was aimed to detect the effects of alcohol on bone metabolism and biomechanical property of growing mice. Thirty KM mice were randomly divided into 3 groups, namely basal control group (mice were killed at the beginning), normal control group (with distilled water given by gastrogavage), and 50% (V/V) alcohol group (with alcohol given by gastrogavage at the dose of 4 g x kg(-1) x d(-1) for 60 days). All mice were killed and their proximal tibia and tibial diaphysis were processed by undecalcified sections and measured by bone histomorphometry. The biomechanical properties of lumbar vertebra and femur were tested. Compared with normal control, the index of trabecular bone area (% Tb. Ar) of proximal tibial metaphysis (PTM) and the static parameter of cortical bone( Ct. Ar) both decreased obviously (P < 0.05) in alcohol group. Bone formation rate (BFR/TV) of trabecular bone and cortical bone dropped also (P < 0.05). The maximal resistibility of lumbar vertebra and structural mechanical strength of proximal femoral neck both declined significantly (P < 0.01) in alcohol group. Low dose of alcohol inhibited the bone formation rate of growing mice , thus leading to a disorder of bone metabolism and a decrease in biomechanical quality.
Animals ; Biomechanical Phenomena ; Bone Density ; drug effects ; Bone and Bones ; drug effects ; metabolism ; Ethanol ; adverse effects ; pharmacology ; Female ; Male ; Mice ; Random Allocation

Occupational silicosis features as irreversible pulmonary fibrosis, which is caused by long-term inhalation of free silica dust. The pathogenesis of silicosis is complex and there is no cure at present. Traditional Chinese medicine classifies silicosis fibrosis into the category of diseases as "pulmonary paralysis" and "pulmonary arthralgia", and its treatment is based on promoting blood circulation and activating qi. Traditional Chinese medicine for activating blood circulation is one of the commonly used medications, which has the effects of anti-oxidation, anti-inflammation, anti-fibrosis and immunomodulation, and has broad application prospect in the prevention and treatment of silicosis. At present, animal experiments and clinical studies have been carried out using the single Chinese herbs extracts that could activate blood circulation such as Salvia miltiorrhiza, Ligusticum chuanxiong Hort., Panax notoginseng, Curcuma longa L., peach kernel and Carthamus tinctorius L. as well as their compound herbs for the prevention and treatment of silicosis. The mechanisms of anti-pulmonary fibrosis and the efficacy and safety of treating silicosis and its complications were explored. There are also scholars studying Salvia miltiorrhiza, Curcuma longa L. and Danhong injection, Taohong Siwu Decoction and others for prevention and treatment of pulmonary fibrosis. Additionally, network pharmacological research, analyzing potential targets and pathways, were carried out to provide scientific rationale for prevention and treatment of silicosis. However, the effectiveness of research is still uncertain, and it cannot meet the clinical needs. In the future, it is necessary to explore the application of more high-quality active components of traditional Chinese medicine monomer or mixture of activating blood circulation in the prevention and treatment of silicosis, to provide new ideas and scientific basis for the prevention and treatment of silicosis using traditional Chinese medicine.

AIM: To observe the changes of macular retinal structure and microcirculation in patients with pituitary adenoma(PA)by optical coherence tomography(OCT)and optical coherence tomography angiography(OCTA).METHODS: Cross-sectional study. A total of 40 PA patients treated at the department of neurosurgery, Affiliated Hospital of Guangdong Medical University from September 2021 to March 2023 were included as PA group, and 42 age- and gender-matched healthy volunteers were selected as normal control group. All patients underwent visual field, OCT and OCTA examinations, and the correlation of ocular parameters in PA patients was analyzed.RESULTS:The vessel density(VD)of each retinal layer in the macular area of the PA group was lower than that of the normal control group, and the superficial vascular complex(SVC)-VD in the macular area was positively correlated with the thickness of the macular ganglion cell complex(mGCC)(except the nasal side of the inner ring and the lower part of the outer ring; P<0.05). The thickness of mGCC in each quadrant of the macular area and the thickness of the circumpapillary retinal nerve fiber layer(CP-RNFL)in each quadrant were negatively correlated with the mean defect(MD)value of the visual field(P<0.05), and the area of the foveal avascular zone(FAZ)was positively correlated with the MD value(P<0.05).CONCLUSION:The combination of OCT and OCTA can fully understand the microscopic changes of retinal structure and microcirculation function in PA patients, which is of great value in evaluating the preoperative visual function of PA patients.

OBJECTIVETo apply the Wright-Giemsa stain in micronucleus test and to explore the stain outcomes of Wright-Giemsa dye of various proportions and staining times.

METHODSUse Wright-Giemsa dye, Wright dye (staining time 3 min) and Giemsa dye (staining time 5 min) to stain HepG2 and then observe the staining effect. The Wright-Giemsa dye was applied under 5 different proportions (3:1-1:3) and different staining times (1, 3, 5, 10, 15 min).

RESULTSAfter stained for 3-5 min with the proportion ratio of 3:1 of Wright-Giemsa dye, the HepG2 cells showed much better staining outcomes compared with the single stain of either Wright or Giemsa.

CONCLUSIONSWright-Giemsa stain can be used in cell micronucleus test to obtain good staining outcomes.

Azure Stains ; Coloring Agents ; Hep G2 Cells ; Humans ; Micronucleus Tests ; Staining and Labeling ; methods

Several digital filtering techniques for correcting baseline drift from ECG signals are presented. A bidirectional filter is introduced, which can cantain baseline drift their filtering performance and realization methods are compared.
Electrocardiography ; instrumentation ; methods ; Filtration ; instrumentation ; Signal Processing, Computer-Assisted ; instrumentation

OBJECTIVETo select a safe, quick and effective method for terminating ectopic pregnancy (EP) with few adverse effects.

METHODSPatients were divided into 2 groups. The observed group was treated with methotrexate (MTX, 50 mg/m2 for single dose intramuscular injection) plus RU 486 (600 mg taken orally in the morning with empty stomach, followed with fasting for 2 hrs) and Waiyun Zhuyu decoction (WZD, one dose a day for 7-10 days). The control group was only treated with MTX, with the same regimen as used in the observed group.

RESULTSThe curative rate, time for blood beta-HCG recovering, lump absorption time, and tube recanalization rate in the observed group were better than those in the control group (P < 0.01). CONCLUSION; The key links for successfully treating EP with conservative drug therpy are to diagnose the disease early and clearly, and to select indicative subject strictly. The scheme of single dose administration of MTX plus RU486 combined with WZD, with its high efficacy and few adverse reaction, may be used as the first choice for referential clinical drugs administration.

Abortifacient Agents, Nonsteroidal ; administration & dosage ; Drug Therapy, Combination ; Drugs, Chinese Herbal ; administration & dosage ; Female ; Follow-Up Studies ; Humans ; Methotrexate ; administration & dosage ; Mifepristone ; administration & dosage ; Phytotherapy ; Pregnancy ; Pregnancy, Ectopic ; drug therapy ; Retrospective Studies ; Treatment Outcome

Bilirubin ; blood ; Breast Feeding ; Evoked Potentials, Auditory, Brain Stem ; Female ; Humans ; Infant, Newborn ; Jaundice, Neonatal ; physiopathology ; therapy ; Male

AIM: To compare the refractive outcomes of femtosecond LASIK with two different flap thickness in the correction of myopia.

METHODS: A total of 236 eyes of 118 myopic patients received LASIK with Wave-Light exeimer laser system. In the non-high myopia group, 30 people(60 eyes)used 90 micron corneal flaps, the other(60 eyes)used 110 micron corneal flaps; in the high myopia group,29 people(58 eyes)used 90 micron corneal flaps,the other(58 eyes)used 110 micron corneal flaps. Uncorrected distance visual acuity(UDVA), corrected distance visual acuity(CDVA), manifest refraction and intraocular pressure before operation, at 1mo, 3mo postoperatively were evaluated.

RESULTS: The percent of postoperative UDVA better than or equal to preoperative CDVA after 3mo: 57 eyes(95%). In the non-high myopia group no matter 90 micron corneal flaps or 110 micron corneal flaps. In the high myopia group, there are 53 eyes(91.4%)with 90 micron corneal flaps and 56 eyes(96.6%)with 110 micron corneal flaps(P=0.438); The percent of spherical equivalent refraction(SE)within ±0.50D of the target refraction change: 51 eyes(85%). In the non-high myopia group no matter 90 micron corneal flaps or 110 micron corneal flaps, 48 eyes(82.8%). In the high myopia group with 90 micron corneal flaps or 110 micron corneal flaps; The percent of change in SE within 0.50D between 1mo and 3mo postoperatively: there are 57 eyes(95%)with 90 micron corneal flaps and 55 eyes(91.7%)with 110 micron corneal flapsin the non-high myopia group(P=0.717). In the high myopia group, there are 44 eyes(75.9%)with 90 micron corneal flaps and 50 eyes(86.2%)with 110 micron corneal(P=0.464).

CONCLUSION: Both 90 micron corneal flaps and 110 micron corneal flaps show similar good efficacy, predictability and stability in the correction of myopia.