Abdominal aortic aneurysm (AAA) and atherosclerosis (AS) have considerable similarities in clinical risk factors and molecular pathogenesis. The aim of our study was to investigate the differences between AAA and AS from the perspective of metabolomics, and to explore the potential mechanisms of differential metabolites via integration analysis with transcriptomics. Plasma samples from 32 AAA and 32 AS patients were applied to characterize the metabolite profiles using untargeted liquid chromatography-mass spectrometry (LC-MS). A total of 18 remarkably different metabolites were identified, and a combination of seven metabolites could potentially serve as a biomarker to distinguish AAA and AS, with an area under the curve (AUC) of 0.93. Subsequently, we analyzed both the metabolomics and transcriptomics data and found that seven metabolites, especially 2'-deoxy-D-ribose (2dDR), were significantly correlated with differentially expressed genes. In conclusion, our study presents a comprehensive landscape of plasma metabolites in AAA and AS patients, and provides a research direction for pathogenetic mechanisms in atherosclerotic AAA.

Objective:To investigate the influencing factors for depression among workers in a petrochemical enterprise and the association of occupational stress and occupational environment with depression, and to provide a scientific basis for the improvement of mental health and the prevention and treatment of depression among workers in petrochemical enterprises.Methods:A cross-sectional study was performed in March 2018, and cluster sampling was used to select 1888 workers from a petrochemical enterprise in Liaoning Province, China for a questionnaire survey. A total of 1888 questionnaires were collected, among which there were 1521 usable questionnaires, with an effective recovery rate of 80.56%. The general status of the workers in this petrochemical enterprise was investigated, and Center for Epidemiologic Studies Depression Scale (CES-D) , Effort-Reward Imbalance (ERI) , and Perceived Work Environment (PWE) were used to evaluate depression, occupational stress, and occupational environment. Constituent ratio was used to describe the distribution of different demographic features, work conditions, and lifestyles among the workers in this petrochemical enterprise. One-way analysis of variance and the t-test were used to compare the scores of depressive tendency between the workers with different features. Pearson correlation analysis was used to investigate the correlation of occupational stress and occupational environment with depression. Multivariate hierarchical regression analysis was used to investigate the influencing factors for depression.Results:The score of depression tendency was 18.94±9.21 in the workers in this petrochemical enterprise, and the incidence rate of depression symptoms was 59.8% (909/1521) . The workers who were aged <35 years, lived alone or in widowhood, or had an income of ≤4000 yuan tended to have a high score of depression tendency. And ordinary workers, oil-refining workers, workers with night shift and non-fixed shift, and workers with a lack of physical exercise and low sleep quality tended to have a high score of depression tendency ( P<0.05) . The dimensions of extrinsic and intrinsic efforts of occupational stress and severity of occupational environment were positively correlated with depression ( r=0.254, 0.242, 0.274, P<0.01) . And the dimension of reward was negatively correlated with depression ( r=-0.348, P<0.01) . The multivariate hierarchical regression analysis showed that occupational stress and occupational environment had a marked predictive effects on depression, with an explained variance of 18.4% and 12.1%, respectively. Conclusion:There is a high level of depression tendency among workers in petrochemical enterprises, and demographic features, work conditions, lifestyle, occupational stressors, and occupational environment factors are influencing factors for depression.

Objective:To investigate the influencing factors for depression among workers in a petrochemical enterprise and the association of occupational stress and occupational environment with depression, and to provide a scientific basis for the improvement of mental health and the prevention and treatment of depression among workers in petrochemical enterprises.Methods:A cross-sectional study was performed in March 2018, and cluster sampling was used to select 1888 workers from a petrochemical enterprise in Liaoning Province, China for a questionnaire survey. A total of 1888 questionnaires were collected, among which there were 1521 usable questionnaires, with an effective recovery rate of 80.56%. The general status of the workers in this petrochemical enterprise was investigated, and Center for Epidemiologic Studies Depression Scale (CES-D) , Effort-Reward Imbalance (ERI) , and Perceived Work Environment (PWE) were used to evaluate depression, occupational stress, and occupational environment. Constituent ratio was used to describe the distribution of different demographic features, work conditions, and lifestyles among the workers in this petrochemical enterprise. One-way analysis of variance and the t-test were used to compare the scores of depressive tendency between the workers with different features. Pearson correlation analysis was used to investigate the correlation of occupational stress and occupational environment with depression. Multivariate hierarchical regression analysis was used to investigate the influencing factors for depression.Results:The score of depression tendency was 18.94±9.21 in the workers in this petrochemical enterprise, and the incidence rate of depression symptoms was 59.8% (909/1521) . The workers who were aged <35 years, lived alone or in widowhood, or had an income of ≤4000 yuan tended to have a high score of depression tendency. And ordinary workers, oil-refining workers, workers with night shift and non-fixed shift, and workers with a lack of physical exercise and low sleep quality tended to have a high score of depression tendency ( P<0.05) . The dimensions of extrinsic and intrinsic efforts of occupational stress and severity of occupational environment were positively correlated with depression ( r=0.254, 0.242, 0.274, P<0.01) . And the dimension of reward was negatively correlated with depression ( r=-0.348, P<0.01) . The multivariate hierarchical regression analysis showed that occupational stress and occupational environment had a marked predictive effects on depression, with an explained variance of 18.4% and 12.1%, respectively. Conclusion:There is a high level of depression tendency among workers in petrochemical enterprises, and demographic features, work conditions, lifestyle, occupational stressors, and occupational environment factors are influencing factors for depression.

Objective : To investigate the effect and mechanism of overexpression of miR⁃370 on immune injury in asthmatic mice induced by ovalbumin ( OVA) . Methods : 40 female BALB/c mice were divided into control group , model group (OVA) , negative control group (OVA + miR⁃NC) and miR⁃370 overexpression group ( OVA+ miR⁃370) . Except for the control group , the other groups were sensitized and stimulated to establish the asthma mouse model. After successful modeling , the OVA + miR⁃NC group and the OVA + miR⁃370 group were injected with miR⁃NC adenovirus and miR⁃370 overexpression adenovirus respectively by caudal vein. RT⁃PCR was commended to detect the expression level of miR⁃370 , the bronchoalveolar lavage fluid ( BALF) was collected , and Wright - Giemsa staining was used to determine the BALF number of leukocytes. Enzyme⁃linked immunosorbent assay (ELISA) was employed to detect the expression levels of immunoglobulin E (IgE) and immunomodulatory factor protein in BALF of asthmatic mice ; hematoxylin⁃eosin (HE) staining and terminal labeling method (TUNEL) were used to observe the degree of lung tissue pathological damage and cell apoptosis ; Western blot was used to detect the protein expression levels of Bax/Bcl⁃2 and cleaved cas3/cas3. Results : Compared with the control group , mice in the OVA group expressed low miR⁃370 (P < 0. 05) , the number of leukagtes increased (P < 0. 05) , the mice in the OVA group expressed low miR⁃370 (P < 0. 05) , the number of leukagtes increased (P < 0. 05) , the ( IL⁃13) , interleukin⁃12 (IL⁃12) and interleukin 4 (IL⁃4) were up⁃regulated (P < 0. 05) , and lung tissue was severely damage(P < 0. 05) , the expression ratio of Bax/Bcl⁃2 and cleaved cas3/cas3 increased (P < 0. 05) ; Compared with the OVA group , there was no difference in the indicators of the OVA + miR⁃NC grouup. Over⁃rexpression of miR⁃370 up⁃regulated the expression of miR⁃370 ( P < 0. 05) , decreased the number of leukagtes( P < 0. 05 ) and the level of IgE (P < 0. 05) , down⁃regulated immunoregulatory factors INF⁃γ and IL⁃12 (P < 0. 05) , up⁃regulated ( IL⁃13) IL⁃13 and IL⁃4(P < 0. 05) , and improved lung tissue damage , decreased cell apoptosis , and reduced the expression ratio of Bax/Bcl⁃2 and cleaved cas3/cas3(P < 0. 05) . Conclusion Overexpression of miR⁃370 inhibits the production of BALF inflammatory cells in OVA⁃induced asthmatic mice and relieves lung injury in mice.