Objective To compare the value of ultrasound and magnetic resonance imaging (MRI) in detecting fetal spine and spinal cord dysplasia Methods Thirty women with complicated pregnancies,age from 22 to 41 years,gestation from 23 - 38 weeks,were studied with MRI within 72 hours after ultrasound studies and revealed 19 cases of fetal spine with irregular,7 cases of vertebral canal widened partly and 4 cases of anomalies of spinal curvature.Autopsy or imaging was conducted when pregnancy was terminated.A close follow-up was given for cases continuing pregnancy within 12 months after delivery.Results (1)Nineteen cases of fetal spine with irregular in ultrasound,the coincidence of antenatal sonographic diagnosis was 42% (8/19),the coincidence of antenatal MRI diagnosis was 89% (17/19).Seven cases of vertebral canal widened partly in ultrasound,the coincidence of antenatal sonographic diagnosis was 0,the coincidence of antenatal MRI diagnosis was 7/7.Four cases of anomalies of spinal curvature in ultrasound,the coincidence of antenatal sonographic diagnosis was 2/4,the coincidence of antenatal MRI diagnosis was 3/4.(2)By MRI study,one cases were conformed their ultrasound diagnosis,eleven cases were completed their ultrasound diagnosis,nine cases were made the same diagnosis as ultrasound and six cases were corrected diagnosis.By MRI and ultrasound study one case of hemivertebra,one case of butterfly vertebra and one case of dorsal dermal sinus were missed diagnosis.Ultrasond had limits and low specificity especially in fetal spinal cord.Conclusions MRI can show the spinal cord and its lesion intuitively and has advantages in displaying fetal anomaly of spine and spinal cord.MRI is a valuable complement to sonography in difficult cases and diagnostic accuracy can be significantly improved.

Objective To observe clinical effect of Danshentong capsule on invisible blood loss after total hip arthroplasty postoperative.Methods 90 cases were randomly divided into observation group and control group,which were chosen from January 2011 to March 2013, 45 cases in each group. Control group received low molecular weight heparin for prevention invisible blood loss, postoperative.Observation group were given Danshentong capsule for prevention invisible blood loss postoperative.Changes of related indicators were followed up and recorded.Results Hemoglobin were (112.20 ±16.17),(124.79 ±11.53)g/L of observation group 3 and 7 days postoperation, were higher than control group (94.84 ±14.93),(103.52 ±10.62) g/L (P<0.05).Patients in observation group 3 and 7 days platelet values (258.31 ±32.95),(242.16 ±31.06) ×109/L, than control group (225.14 ±30.48,214.95 ±28.68) ×109/L (P<0.05).Patients in observation group hidden hemorrhage (420.59 ±53.96), lower than control group (868.05 ±65.37) U/mL (P<0.05).Postoperative adverse reactions observed ratio of 6.67% in observation group, lower than control group 26.67%(P<0.05).Conclusion Danshentong capsule in hip replacement surgery occult bleeding prevention clinical exact effect, no significant side effects, is worthy of further research and application.

Objective To compare the effects of Chemiluminescence immunoassay ( CLIA) and ELISA for detecting the HIV antibody in order to explore the feasibility of CLIA.Methods 3,542 AIDS high-risk people were randomly selected from February 2013 to March 2015 in our city, the serum was collected and HIV antibodies in the serum were detected by CLIA and ELISA.The detection results were confirmed by the Western blot.Results By ELISA assay, 56 serum samples were positive with a positive rate of 1.58%, while CLIA 61 were positive with a pos-itive rate of 1.72%.The detection results on 11 serum samples were inconsistent among the 3,542 serum samples by ELISA and CLIA.The positive predictive value of CLIA was 96.72%, the detection sensitivity 98.33%and specific-ity 99.94%, while the positive predictive value of ELISA was 96.43%, the detection sensitivity 90.00%and speci-ficity 99.94%.The positive predictive value, sensitivity, specificity and other indicators showed no significant differ-ence between ELISA and CLIA(P>0.05).Conclusion CLIA shows high sensitivity, specificity and accuracy and therefore it can be used for detecting HIV antibody.

Genetic factors are important causes of male infertility and account for about 30%infertility cases.Therefore, it is necessary to carry out molecular and genetic detections in the diagnosis and treatment of male infertility.Here the most commonly used techniques for molecular and genetic diagnosis of male infertility in recent years are discussed , including chromosomal abnormities analysis , Y chromosome microdeletions detection , gene mutation screening and sperm quality and function examination.

Objective To investigate the value of the flow cytometry(FCM)for detecting platelet associated antibody in the di-agnosis of immune thrombocytopenia(ITP).Methods Platelet associated antibody,expression rate and the fluorescence intensity of platelet-associated immunoglobulin(PAIg)were measured in 51 patients with ITP(23 cases of newly diagnosed ITP and 28 cases of persistent ITP),21 patients with non-ITP and 60 healthy individuals.The correlation between the detection results with the platelet and megakaryocyte counts was performed;the expression rates of PAIg before and after treatment were compared.Results The fluorescence positive percentage and mean fluorescence intensity of IgG,Ig A and IgM in the patients group were significantly high-er than those in the control group with statistical difference(P <0.01).Compared with the non-ITP group,the difference had statis-tical significance(P <0.05).Compared with the autoimmune disease group,the difference had no statistical significance(P >0.05);the difference between the autoimmune disease group and the healthy control group had statistical significance(P <0.01 );in the persistent ITP group,PAIg in the complete response group had statistical difference between the groups before treament.PAIg in the response group also had statistical difference between the groups before treament (P <0.05).Conclusion FCM for detecting PAIg can be used in the diagnosis,differential diagnosis and the disease condition monitoring of ITP.

Objective To evaluate the ability of diffusion tensor imaging (DTI )and DTT to differentiate the central prostate cancer(PCa )from benign prostate hyperplasia (BPH ).Methods 16 CG-PCa cases and 30 BPH cases proven by Histopathologi-cally had been performed MRI and DTI.ADC values and FA values of CG-PCa and BPH were respectively measured,comparing whether there is any discrepancies between two groups.For DTT maps,two observers record the score using a four-point scale and compare the differences between two groups of lesions about fibers continuity and density.Results ADC values and FA values in BPH-PCa were:(1 357±163)×10-6-(1 058±196)×10-6 mm2/s,and (356±116)×10-3- (407±132)×10-3 respectively. ADC value between the two groups was statistically significant difference (P< 0.05).While FA values was not(P>0.05).Interob-server agreement regarding the scale was good (k =0.723).The two viewers'DTT map score of CG cancer were 2.9±0.9;2.8± 1.0,the BPH were 1.8±0.7;1.7±0.8.The DTT map score between the two groups for two viewers were all statistically signifi-cant difference (P< 0.05).Conclusion ADC values can significantly differentiate CG-PCa from BPH,FA values cannot.DTT maps can describe prostate more institute,providing a certain value in distinguish PCa from BPH.

Objective To assess the clinical features of female patients with AMI and their out-comes .Methods Two hundred and eighty-four AMI patients (216 males and 68 females) were in-cluded in this study .Their clinical features ,complications ,PCI and outcomes were comared .The patients were followed up for 3 months ,during which the major adverse cardiac events (MACE) were observed with their mortality and risk factors for MACE assessed .Results The age was ol-der and the heart rate ,TIMI grade ,GRACE score were significantly higher in female patients than in male patients (P<0 .05 ,P<0 .01) .The ratio of female patients who underwent PCI was signif-icantly lower than that of male patients who underwent PCI (30 .9% vs 48 .6% ,P=0 .012) .The in hospital mortality was higher in female patients than in male patients (17 .6% vs 9 .3% ,P=0.077) ,and the incidence of MACE in a 3-month follow-up period was significantly higher in fe-male patients than in male patients (27 .9% vs 15 .7% ,P=0.032) .Multivariate logistic regression analysis showed that age and Killip classification were the independent risk factors for in hospital mortality (OR=1 .078 ,95% CI:1 .035 -1 .123 ,P=0 .000 ;OR= 1 .901 ,95% CI:1 .373 -2 .633 , P=0 .000) and for MACE in a 3-month follow-up period (OR=1 .040 ,95% CI:1 .008 -1 .074 , P=0 .015;OR=1 .543 ,95% CI:1 .170-2 .034 ,P=0 .002) .PCI was a protective factor for MACE in a 3-month follow-up period (OR=0 .090 ,95% CI:0 .026-0 .306 ,P=0 .000) .Conclusion The age of female AMI patients is older and their heart function is poorer with a higher risk of MACE and a less chance for PCI .However ,gender itself is not an independent predictor for in hospital mortality and M ACE in a 3-month follow-up period .

Objective To analyze the diagnostic value of diffusion tensor imaging(DTI)and diffusion tensor tractography(DTT) in viral encephalitis at 3.0T magneton. Methods 25 patients with viral encephalitis confirmed by clinic and 25 age-match healthy patients were examined by conventional MRI and DTI. The average diffusion coefficient (ADC) map, fractional anisotropy (FA) map and diffusion tensor tractography (DTT) was acquired through image post processing technique. The ADC value, FA value of lesions and normal tissues were measured by region of interest (ROI) with the same standard and the results were statistically analyzed.Results In the acute stage of viral encephalitis: The ADC values were significantly lower than that in normal tissues of control group (P<0.01), FA values of lesions were higher than that of control group and had no statistical significance (P＞0.05). In the subacute and chronic stage of viral encephalitis: The ADC values were higher than that of control group and had statistical significance(P＜0.01), FA values of lesions were significantly lower than that of control group(P<0.05). DTT imaging showed infiltrative changes of white matter fibers in the lesion areas.Conclusion DTI and DTT technique may provide some complementally informations and clinical diagnostic value for viral encephalitis.

BACKGROUND: Although single gene can induce bone marrow mesenchymal stem cells (BMSCs) into chondrocyte, it still has limit action, polygene interaction accord with real demand of body internal environment and is helpful to play synergistic effect and to improve the differentiated effect.OBJECTIVE: To investigate the secretion of target gene and differentiation of BMSCs transfected by BOX-9 and insulin-like growth factor-1 (IGF-1) gene alone and together into chondrocytes.DESIGN, TIME AND SETTING: Two-specimen observation was conducted in the Cell Biological Laboratory of China Medical University between April 2008 and February 2009. MATERIALS: Two 6-week-old healthy male Wistar rats were used for the BMSCs extraction. METHODS: The plasmids pcDNA3.1-1GF-1 and pcDNA3.1- BOX-9 were amplified and extracted. BMSCs of Wistar rats were separated and purified. According to the transfect situation, the BMSCs were divided into 5 groups. ①Non-transfected group:serum-free and double-antibody-free L-DMEM; ②empty vector transfection group: serum-free and double-antibody-free L-DMEM + pcDNA3.1 empty vector and liposome; ③BOX-9 transfection group: serum-free and double-antibody-free L-DMEM +pcDNA3.1-SOX-9 plasmid and liposome; ④IGF-1 transfection group: serum-free and double-antibody-free L-DMEM+pcDNA3.1-1GF-1 plasmid and liposome; ⑤SOX-9 and IGF-1 transfection group: serum-free and double-antibody-free L-DMEM+ pcDNA3.1-1GF-1 plasmid and liposome, pcDNA3.1-SOX-9 plasmid and liposome.MAIN OUTCOME MEASURES: After being transfected, the cells were selected, the proliferation activity was tested by MTT and expression levels of target gene and protein were tested by RT-PCR and Western blot. RESULTS: The proliferation activity (A value) of BMSCs transfected by SOX-9 alone, iGF-1 alone, SOX-9 and IGF-1 together was significantly higher than that of BMSCs in the non-transfected group and empty vector trensfection group (P < 0.01). RT-PCR and Western blot result showed that SOX-9 expression was the highest in the group transfected by SOX-9, the expression of IGF-1 was the highest in the group transfected both by SOX-9 and IGF-1, and the expression of collagen Ⅱ was the highest in the group transfected both by SOX-9 and IGF-1.CONCLUSION: The double gene transfection is supedor to single transfection with regard to the differentiation of BMSCs to chondrocytes and the secretion of extracallular matdx. It is indirectly suggested that IGF-1 induces BMSCs to differentiate into chondrocytes, but the capacity is less than SOX-9.

Objective To investigate the changes of the white matter in adolescent depression by using the method of tract-based spatial statistics (TBSS). Methods We employed TBSS to examine WM microstructure in 35 treatment-naive adolescents with clinical depression and in 40 matched controls. By using the TBSS, we compared the difference of fractional anisotropy (FA), axial diffusivity (AD), radial diffusivity (RD) and mean diffusivity (MD) between theadolescent patients with depression and the controls. Results Our analysis revealed the abnormal WM microstructures in the clinically depressed adolescents. The whole-brain analysis revealed that patients, with lower FA values in the body of the corpus callosum (CC) (P < 0.01), had elevated RD and MD (P < 0.01), and preserved AD (P > 0.05). The FA values in the body of the corpus callosum was negatively correlated with the severity of depression (P < 0.01). Conclusions Our findings suggest that WM abnormalities are involved in the path-physiology of depression. Importantly , our findings show that these WM abnormalities present early in the course of the disorder.