Objective To explore the role of the transforming growth factor-?1 (TGF-?1) and the vascular endothelial growth factor (VEGF) in the occurrence and development of the pulmonary fibrosis as well as the synthesis and distribution of these two factors in relation to the angiogenesis process of the pulmonary tissues. Methods The rats were randomly assigned to control group (CG) and bleomycin group (BLM) with the same physical conditions. The bleomycin was introduced to the lung tissues of the rats in the BLM group to mimic the pulmonary fibrosis process. The synthesis and distribution of VEGF and TGF-?1 were observed and recorded on day 3, 7, 14, and 28 in both groups by the immunohistochemical and in situ hybridization approaches. The pathological changes of the subjects were also observed and recorded using both the light-microscope and the transmission electron microscope (TEM) on the same dates.Results The BLM group demonstrated the great significance in both of the VEGF and TGF-?1 presentation and the pathological changes in the pulmonary tissues comparing to the CG group. In the BLM group, VEGF and TGF-?1presented dramatically in the early stages of the fibrosis and this phenomenon lasted with the parallel increases of both the factors. This presentation of VEGF and TGF-?1 showed the greatest distribution on day 28 in the lung interstitial cells and the areas of the presentation corresponded to that of the new angiogenesis and the fibrohyperplacia of the pulmonary tissues. The endothelial cells of the lung capillaries were observed to demonstrate early necrosis, separation from the base, and increased penetrance followed by a great amount of angiogenesis with twisted new blood capillaries and blood embolism formation within, which continued to show on day 28 in the BLM group.Conclusion The presentation of VEGF and TGF-?1 is highly corresponded with the angiogenesis of the bleomycin treated pulmonary tissues in the rats. The continued significant presentation of these two factors may relate to the damage of the new formed capillaries or the impairment of the pulmonary tissues in rats.

Objective To analyze the MRI characteristics of cavernous angioma of the epidural space in spine.Methods MR imaging features of cavernous angioma of epidural space in 3 cases confirmed by surgery-pathology were analysed with literature review . Results Among the three cases,the tumors located in the lumbar spine in one and in the thoracic spine in 2.The tumors were all at posterior to the spinal cord and shuttle-shaped,the major axis was consistent with the spinal ordinate axis,the corresponding plane spinal cords were compressed and resulting in distortion and shift.The tumors were high signal intensity on T_2WI,and obviously homogeneous enhanced after Gd-DTPA administration. Conclusion MRI examination is the best method to diagnose this kind of disease.

0.05).Early pathology phenotype of BLM groups was similar as those of pneumonia groups.But in day 14th and day 28th groups,the ratio of macrophagus positive area in lung interstitium was bigger than that of control group(P

Objective To investigate the correlation between serum HCY (Homocysteine) and carotid artery stenosis,plaque stability in patients with ischemic cerebrovascular disease.Methods 154 patients with ischemic cerebrovascular disease in Tangdu Hospital were enrolled in the study from June to December 2016.The serum levels of HCY were detected.CT angiography (CTA) was uesd for patients with neck vascular scanning.According to the difference of serum HCY level,patients were divided into 80 cases of high HCY group (observation group) and 74 cases of normal HCY group (control group).The degree of carotid artery stenosis,number and stability of plaque were compared between the two groups and the correlation between serum HCY level and degree of carotid artery stenosis and plaque stability were analyzed.Results The total stenosis rate in the observation group was significantly higher than that in the control group,the moderate stenosis rate and severe stenosis rate in the observation group were significantly higher than those in the control group,with the statistically significant differences (x2 =5.594～ 22.506,all P＜0.05).The levels of serum HCY in mild,moderate and severe stenosis group were 13.16 ± 6.73,15.19± 5.93 and 26.13 ±11.18 μmol/L respectively.The levels of H CY in moderate stenosis group and severe stenosis group were significantly higher than that in mild stenosis group,and the levels of HCY in severe stenosis group was significantly higher than that in moderate stenosis group,with the statistically significant differences (t=2.684～ 5.270,all P＜0.01).The rate of carotid plaque in the observation group was significantly higher than that in the control group,and the differences statistically significant (x2 =25.053,P＜0.01).The rate of unstable plaque and mixed plaque in the observation group was significantly higher than that in the control group,and the rate of stable plaque was significantly lower than that in the control group (x2 =4.067～ 14.95,all P＜0.05).The levels of serum HCY in stable plaque group,mixed plaque group and unstable plaque group were 16.14±5.49,21.91 ± 6.32 and 26.74 ± 10.59 μmol/L respectively.The levels of HCY in mixed plaque group and unstable plaque group were significantly higher than that in stable plaque group,and the differences were statistically significant (t=4.370,4.628,all P＜0.01).The level of HCY in unstable plaque group was significantly higher than that in mixed plaque group,and the difference was statistically significant (t =2.249,P＜ 0.05).Conclusion Serum HCY levels were closely related to carotid artery stenosis and plaque stability.Hyperhomocysteinemia can increase the incidence and degree of carotid artery stenosis as well as the number of carotid plaques and unstable plaques.

Objective To provide theoretical evidences for diagnosis of bladder neoplasm by using more information of imaging features. Methods MRI images of bladder neoplasm were collected for this research. The ROI (region of interest) area was selected manually,and noise reduction and sharpening were applied to the ROI area by using LoG (Laplacian of Gaussian) filter. The texture features of bladder neoplasm and normal bladder wall (smooth muscle),such as mean grey-level intensity,entropy,uniformity were calculated. A statistical analysis was made at last. Results The values of texture features were analyzed by t-test. Entropy and uniformity show significant differences between the two groups. But Mean grey-level intensity hasn't indicated this difference. Conclusion This texture features may be applied to decide the invasive depth of bladder neoplasm,it also means that the stage of bladder neoplasm may be fixed by this system.

Objective To investigate the correlation between HCY (Homocysteine),folate,vitamin B12 and head and neck vascular stenosis in patients with ischemic cerebrovascular disease.Methods 225 patients with ischemic cerebrovascular disease in Tangdu Hospital of the Fourth Military Medical University were enrolled in the study from April 2016 to October 2016.The serum levels of HCY,folate and vitamin B12 were detected.CT angiography (CTA) was uesd for patients with head and neck vascular scanning.According to whether the presence of vascular stenosis,patients were classified as no vessel stenosis group and vascular stenosis group.According to the degree of stenosis,patients were classified as no vascular stenosis group,mild moderate stenosis group and severe stenosis group.Results The HCY levels in the vascular stenosis group were significantly higher than no vessel stenosis group,while the levels of folate and vitamin B12 were significantly lower than no vessel stenosis group,the differences were statistically significant (t=9.656,7.140 and 8.350,all P＜0,01).The HCY levels in mild moderate stenosis group and severe stenosis group were significantly higher than no vessel stenosis group,and the HCY levels in severe stenosis group were significantly higher than mild moderate stenosis group,the differences were statistically significant (t=6.108,9.401 and 5.273,all P＜0.01).The folate levels in mild moderate stenosis group and severe stenosis group were significantly lower than no stenosis group,the differences were statistically significant (t=5.574 and 5.988,all P＜0.01).The vitamin B12 levels in mild moderate stenosis group and severe stenosis group were significantly lower than no stenosis group,the differences were statistically significant (t=4.548 and 7.816,all P＜0.01).The degree of head and neck vascular stenosis and serum levels of HCY were positively correlated (r=0.331,P＜0.01).The degree of head and neck vascular stenosis and levels of vitamin B12 were negatively correlated (r=-0.279,P＜0.05).Conclusion The levels of HCY,folate and vitamin tB12 were closely related to the degree of head and neck vascular stenosis.HCY,folate,vitamin B12 and head and neck CTA play important roles in patients with ischemic cerebrovascular disease clinically.

Objective To investigate the CT characteristics of retroperitoneal extra-adrenal paraganglioma(PGL).Methods CT findings of 11 patients with pathologically confirmed retroperitoneal extra-adrenal PGL were analyzed retrospectively,and the relevant literature was reviewed.All patients received plain CT as well as contrast-enhancement CT scan.Results All of the 11 tumors were located in the retroperitoneal space around large blood vessels,including 2 functional and 9 unfunctional extra-adrenal paragangliomas.Eight of 11 cases were benign tumors which presented as round or oval masses with sharp margins,separated from surrounding structures clearly;while the rest 3 cases,which with indistinct edge,even more with the invasion of surrounding structures (renal artery,renal venous and pancreas),were confirmed to be malignant.Most of the tumors were heterogeneous on plain CT.It was common to see intratumoral necrosis or cystic degeneration in the tumors (9 cases),while hemorrhage or calcification was uncommon,which was found in 3 and 2 cases,respectively.Homogeneous intensity was only observed in 2 masses with diameter less than 4 cm.On contrast-enhanced CT image, the solid part of the tumors presented continuously significant enhancement.Dilated feeding artery was found in or around the masses (8 cases) in artery phase.Conclusion There are certain characteristics of retroperitoneal extra-adrenal paraganglioma on CT scanning, which are helpful for preoperative diagnosis and treatment of this disease.

Objective:To explore the expression of CD23,CD19 on peripheral blood lymphocytes as well as its association with serum total IgE levels and nasal allergic symptoms in patients with allergic rhinitis(AR). Method:Symptom scores were evaluated in 46 AR patients,expression of CD23,CD19 on peripheral blood lymphocytes were measured by flow cytometry,and serum total IgE levels were determined by immune chemiluminescence. Thirty two healthy individuals were enrolled as controls.Result:The percentage of CD23~+,CD19~+ and CD23~+/CD19~+,on peripheral blood lymphocytes in AR patients were 11.6±1.9,22.8±3.3,10.2±1.7,respectively.Higher frequencies of CD23~+,CD19~+,and CD23~+/CD19~+ were found in AR patients compared with controls (P＜0.05).There were positive correlations between expression rates of CD23~+,CD19~+,CD23~+/CD19~+ and levels of serum total IgE,nasal allergi symptom scores,respectively.CD23~+ /CD19~+ demonstrated greater correlations with serum total IgE and nasal allergic symptom(r=0.65 and 0.49,P＜0.05)than CD23~+ and CD19~+ did.Correlation between CD23~+/CD19~+ and nasal allergic symptom scores was greater than the corresponding correlations of serum total IgE(r=0.33,P＜0.05).Conclusion:CD23 and CD19 are important factors that associated with serum total IgE in the pathogenesis of AR,Analysis on the expression of CD23~+/CD19~+ on peripheral blood lymphocytes is helpful for evaluating the severity of AR.

Objective To investigate the expression and clinical significance of HGF and C-Met in cholangiocarcinoma .Meth-ods The levels of HGF and C-Met were measured by ELISA ,the expression of HGF and C-Met in cholangiocarcinoma and health control was detected by immunohistochemistry .Results The preoperative levels of HGF and C-Met in cholangiocarcinoma were higher than the health control(P< 0 .05) .Compared with the preoperative state ,the levels of HGF and C-Met in cholangiocarcino-ma on the 3rd day after operation did not change significantly .However ,the levels of HGF and C-Met on the postoperative 7th and 10th day decreased obviously .In addition ,the expression of HGF and C-Met in cholangiocarcinoma was 76 .2% ,81 .0% ,respective-ly ,which is higher than that in normal tissue ( P < 0 .01) .Conclusion There is an abnormal expression of HGF and C-Met in cholangiocarcinoma ,which may be related to the incidence and development of cholangiocarcinoma .

Objective To investigate the signaling cascades involved in the regulatory effects of reactive oxygen species(ROS)on adiponectin expression in 3T3-L1 adipocytes.Methods 3T3-L1 cells were cultured and differentiated into mature adipocytes.Adiponectin mRNA expression was evaluated by quantitative real-time PCR.The signaling pathways associated with ROS-decreased adiponectin expression were screened by Bioplex phosphoprotein assays and various protein kinase inhibitors.Results As an important ROS,H_2O_2activated several signaling pathways including extracellular signal regulated protein kinase 1/2(ERK1/2),c-Jun N-terminal kinase (JNK),protein kinase B(Akt),p70 S6 kinase(p70 S6K),and Janus kinase/signal transducer and activator of transcription(JAK/STAT).Akt and JAK/STAT inhibitors completely reversed H_2O_2-decreased adiponectin expression(both P<0.01).Conclusions ROS markedly down-regulates adiponeetin expression in 313-L1 adipocytes by activating Akt and JAK/STAT signaling pathways,which may contribute to the development of obesity and its related diseases.