Combined Modality Therapy ; Early Diagnosis ; Humans ; Mouth Neoplasms ; diagnosis ; therapy

Animals ; Botulinum Toxins, Type A ; pharmacology ; Capsaicin ; pharmacology ; Carbachol ; pharmacology ; Humans ; Keratoconjunctivitis ; surgery ; Phenylephrine ; pharmacology ; Saliva ; drug effects ; Submandibular Gland ; drug effects ; transplantation ; Translational Medical Research

Humans ; Methods ; Parotid Neoplasms ; surgery

Humans ; Saliva ; physiology ; Salivary Gland Diseases

Humans ; Practice Guidelines as Topic ; Submandibular Gland ; transplantation ; Transplantation, Autologous

Facial Nerve Injuries ; rehabilitation ; surgery ; Humans

Adolescent ; Adult ; Aged ; Aged, 80 and over ; Blood Vessels ; injuries ; Child ; Child, Preschool ; Female ; Free Tissue Flaps ; blood supply ; Head ; surgery ; Humans ; Male ; Middle Aged ; Neck ; surgery ; Tissue Transplantation ; adverse effects ; methods ; Young Adult

Humans ; Lymph Nodes ; pathology ; surgery ; Neck Dissection ; methods ; Oropharyngeal Neoplasms ; pathology ; surgery

Exons ; Female ; Follow-Up Studies ; Hepatocyte Nuclear Factor 1-beta ; genetics ; Humans ; Kidney Diseases, Cystic ; diagnostic imaging ; genetics ; pathology ; surgery ; Kidney Glomerulus ; pathology ; Middle Aged ; Radiography

A novel nucleic acid amplification method,termed loop-mediated isothermal amplification(LAMP),which amplifies DNA with high specificity,efficiency,and rapidity under isothermal conditions,may be a valuable tool for the rapid detection of infectious diseases.This method employs a DNA polymerase that have activity of strand displacement DNA synthesis and a set of four specially designed primers that recognize a total of six distinct sequences on the target DNA.LAMP can amplify a few copies of DNA to 109 in less than an hour.The final products are stem-loop DNA with several inverted repeats of the target and cauliflower-like structures with multiple loops.A positive reaction would be shown as a ladder-like pattern in a gel electrophoresis analysis.Because of the advantage,the LAMP method will be widely applied to research of nucleic acid,clinical diagnosis of infectious diseases and detection of genetically modified organisms etc.