Objective To explore the features of gallbladder adenomyoma in sonogram, and improve the abilities of diagnosis and differential diagnosis. Method Sonograms of 8 patients with proven gallbladder adenomyoma were retrospectively analyzed and correlated with pathologic findings. Results Eight patients had sonographic evidence of localized intramural nodular,which were round-like and hypoechoic,with anechoic space and tiny bright echos, confined almost exclusively to the fundus of gallbladder. Five patients were also evident with associated gallstones and chronic cholecytitis. Conclusion Sonograms of gallbladder adenomyoma have marked features, which can be differentially diagnosed from other projective lesions of gallbladder.

Objective To study the diagnostic value of color Doppler ultrasonography in hematoma of soft tissues, and compare the images of different period of hematoma. Method Analyzed and compared the size,shape and sonographic characteristic of 46 cases with hematoma. Results The images of hematoma were round or oval in shape, more than 2.0 cm in trans-diameter, and were isoechoic or hypocchoic in the early stage, then anoechoic areas increased with hyperechoic septum and irregular hypoechoic, with posterior enhancement and hyperechoic boundary. A part of muscle fibres raptured and were occupied by the hematoma if the muscle tore. Conclusion Color Doppler ultrasonography can be the first choice and reliable imaging method to diagnose hematoma of soft tissues.

Most of the soil microorganisms are unculturable,which limits the exploitation and application of microbial resources.Metagenomics has great potential in the research of unculturable microorganisms in soil.Progress on the research of metagenomic DNA purification,metagenomic library construction and screening of soil microorganisms are reviewed briefly.

The roles of chemokine and chemokine receptor family in immune diseases are drawing increasing attention, as they are very important in maintaining cell survival, inducing gene expression, directly guiding cell migration and regulating immunity. The paper reviews the expression and possible roles of chemokine receptor CXCR3 and its ligands in immune diseases, as well as the latest therapies targeting them.

Background The palate is differently regulated and developed along the anterior-posterior axis. The Bmp signal pathway plays a crucial role in palatogenesis. Conditioned-inactivation of Bmp type I receptor Alk2 or Alk3 in the neural crest or craniofacial region leads to palatal cleft in mice. However, how different Bmp members are involved in palatogenesis remains to be elucidated. In the present study, mRNA expression patterns of Bmp2, Bmp3 and Bmp4 in the developing anterior and posterior palates were examined and compared, focusing on the fusion stage. Methods To detect the expression of Bmp mRNA, antisense riboprobes were synthesized by in vitro transcription. Radioactive in situ hybridization was performed on sagital and coronal sections of mice head from E13 to E18. Results The expression of these Bmps were developmentally regulated in the anterior and posterior palates prior to, during and after palatal fusion. During palatal fusion, Bmp4 expression shifted from the anterior to the posterior palate, Bmp2 was highly expressed in both the anterior and posterior palates in this process, whereas Bmp3 was only localized in the posterior palate. They showed generally non-overlapping pattern in their expression domains. Thereafter, their expression was detected in both the anterior and posterior palates regulating osteogenesis and myogenesis respectively. Conclusions Bmp signalling is involved in palatogenesis in multiple stages and has multiple roles in regulating anterior and posterior palatal development. Disturbances of Bmp signalling during palatogenesis might be a possible mechanism of cleft palate.

Objective To introduce and evaluate the procedure of suprapubic tensionless Sparc TM sling system in treating female stress urinary incontinence (SUI). Methods A total of 42 patients (mean age,54 years) diagnosed with SUI (a mean course of 12 years) were treated by Sparc TM sling method.Of them 4 cases had detrusor instability.Under local anesthesia,this procedure took about a mean of 20 min.The urinary catheters were removed the next morning after the procedure.The patients were counseled on abstaining from heavy lifting,exercises and intercourse for 4 weeks. Results After removal of catheters,all the 42 patients successfully voided without incontinence.Detrusor instability to various degrees developed in 7 patients and was relieved by expectant treatment.All the patients were followed up for 3 to 12 months (mean,6 months).Neither recurrence of SUI nor lower urinary tract obstruction was found.No implant related complications such as local irritation,extrusion,erosion through the urethra,migration from the desired location,fistula formation and inflammation occurred. Conclusions Sparc TM sling system is a simple,safe and efficacious method of treating SUI under local anesthesia.

[Summary] Limb numbness is a common symptom of diabetic peripheral neuropathy(DPN),which is similar to lumbar vertebra disease and spinal cord tumor compression syndrome. Diabetes mellitus (DM) combined with lumbar spondylosis and spinal cord tumor compression is rarely occurred and is difficult to distinguish from DPN . Here we reported a case of DM combined with spinal neurilemoma that was misdiagnosed as DPN for half a year.

A total of 62 cases with autogenous arteriovenous fistulas were examined bv color Doppler ultrasonography.Venous thrombotic manifestations (n =13) and large local hematoma (n =3) were detected.The diameter ranges were around (1.8 ± 0.7) cm for arteriovenous fistula and (2.0 ± 0.8) cm for local hematoma.And there was no statistical significance (t =1.63,P ＞ 0.05).According to edge shape,presence or absence of flow signal and fistula searching,it could be used to identify venous thrombosis and local hematoma of arteriovenous fistula.Color Doppler ultrasound is helpful for the differential diagnosis and the mass properties of arteriovenous fistula so that it provides useful diagnostic information for the clinicians.

OBJECTIVE: To investigate the molecular mechanism of Huanglian Jiedu Decoction (HLJDD), a traditional Chinese compound herbal medicine, in treating type 2 diabetes mellitus (T2DM) by observing its effects on glucose transporter 4 (GLUT4) protein expression and translocation in adipose and skeletal muscle tissues of rats with T2DM. METHODS: T2DM was induced in rats by intravenous injection of a small dose of streptozotocin (STZ, 30 mg/kg) plus high fat and high caloric laboratory chow. Then animals were divided into untreated group, aspirin-treated group and HLJDD-treated group. Normal rats fed with common chow were designated as normal control group. Ten weeks later, the oral glucose tolerance test (OGTT) was performed in all animals, and the changes of murine body weight, fasting blood glucose (FBG), fasting serum insulin (FINS) and the expression of GLUT4 protein in skeletal muscle and adipose tissues before and after insulin treatment were routinely determined. RESULTS: Compared with the untreated group, the result of OGTT of HLJDD-treated group was improved. The levels of the body weight and FBG were decreased, while the GLUT4 protein expression and translocation were elevated obviously. CONCLUSION: It is suggested by the present results that the therapeutic effects of HLJDD on T2DM might be related to its ability of increasing GLUT4 protein expression and translocation in adipose and skeletal muscle tissues of T2DM rats.

Cell cycle-related kinase (CCRK) as a novel CDK-activating kinase plays a significant role in cell cycle regulation.Related studies confirm that CCRK is overexpressed in various tumors,and is associated with poor prognosis of the patients,which indicates that CCRK takes part in the generation and development of the tumor.