1.Pathological ultrastructure changes of lung tissue in diabetic rat and the preventive effect of rosiglitazone intervention
Weixin MU ; Yaxin SHEN ; Hong ZHOU ; Jing HUANG ; Mian WANG ; Guang LIU
Clinical Medicine of China 2011;27(7):700-702
Objective To observe the pathological infrastructure changes of lung tissue in diabetic rat with pulmonary fibrosis and the preventive effect of rosiglitazone intervention. Methods The experimental type 2 diabetic rats were yielded by high-sugar, high fat diet plus intraperitoneal injecting streptozotocin (STZ). The pathological infrastructure changes of lung tissue were observed by transmission electron microscopy in the control, diabetes mellitus group and rosiglitazone treatment group respectively, and the hydroxyproline contentswere determined simultaneously. Results We observed accumulation of collagen fibers and extracellular matrix in the lung tissue of simple diabetic rat, which indicated pulmonary fibrosis. The hydroxyproline contents were significantly higher in diatetes mellitus group when compared to the control at 12 weeks[ 0. 77 ± 0. 03μg/(mg·prot) vs.0.63 ±0.03 μg/(mg·prot)] and 20 weeks [0.93 ±0. 10 μg/(mg·prot) vs.0.87 ±0.10μg/ (mg·prot) ]. (Ps < 0.05) , but the rosiglitazone intervention significantly reduced the hydroxyproline 3contents in diabetes mellitus rats [0.85 ±0.08 μg/(mg·prot) vs.0.87 ±0. 10 μg/(mg·prot) ,P<0.05].Conclusion Pulmonary fibrosis occurred in diabetic rats lung tissue,while rosiglitazone can alleviate it.
2.Study on Rehabilitating Effect of Acupuncture plus Kinetotherapy for Early Hemiplegia
Huamei ZHANG ; Chunhuan WU ; Zhenbang SONG ; Guang ZHAO ; Xuehua SHEN ; Guoqi HUANG
Journal of Acupuncture and Tuina Science 2006;4(6):342-346
To investigate the rehabilitating effect of acupuncture plus kinetotherapy for early hemiplegia due to cerebral apoplexy. Methods:Fifty cases of apoplectic hemiplegia were treated by acupuncture plus kinetotherapy and were compared with another fifty cases treated by single medications,in the therapeutic effect and for follow-up observation. Results:After the treatment for one month on average,the remarkable effective rate was 82% in the rehabilitation group and 54% in the control group,in a significant difference (P<0.05). After the treatment,the scores in the motor function of the limbs and daily life ability were obviously increased in the rehabilitation group,and there was a significant difference in comparison with the control group (P<0.05). Moreover,there was a good regulatory effect on blood rheology and blood lipid in the rehabilitation group. Conclusion:A combination of acupuncture and kinetotherapy has a better effect on apoplectic hemiplegia than single medications.
4.Research wilt disease of Salvia miltiorrhiza and its pathogen.
Li YANG ; Zuo-Qing MIAO ; Guang YANG ; Ai-Juan SHAO ; Lu-Qi HUANG ; Ye SHEN ; Xue WANG ; Mei-Lan CHEN
China Journal of Chinese Materia Medica 2013;38(23):4040-4043
Salvia miltiorrhiza is a highly valued traditional chinese medicine for the treatment of atherosclerosis-related disorders in china, such as cardiovascular and cerebrovascular diseases in China. The wilt disease is serious in the culture of S. miltiorrhiza. Wilt disease cause biomass of plant shoots and roots is lessened, active components are decreased. To solve these problems, we research the pathogen causing wilt disease of S. miltiorrhiza. The suspected pathogen is identified by morphology and etiological test. The identification was further confirmed by alignment the sequences of internal transcribed spacer (ITS) amplified by PCR. Our result show the wilt disease of S. miltiorrhiza mostly occurred in July and August, which is hot and wetter. The wilt disease rate of S. miltiorrhiza continuous cropping for one year in S. miltiorrhiz stubble is 10%, but the wilt disease rate of S. miltiorrhiza continuous cropping for three years in S. miltiorrhiz stubble is 60%-70%. The root rot of S. miltiorrhiz caused by the wilt disease, so the wilt disease was mistaken for the rot root in production. Morphological characteristics show the pathogen is Fusarium oxysporum. The sequence of ITS wes determined and found by BLAST shared 99% identity to that of F. oxysporum f. sp. cucumerinum. So it comes to the conclusion that the causing agent of wilt disease on S. miltiorrhiza belongs to F. oxysporum.
DNA, Intergenic
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genetics
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Fusarium
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genetics
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isolation & purification
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physiology
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Plant Diseases
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microbiology
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Polymerase Chain Reaction
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Salvia miltiorrhiza
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microbiology
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Seasons
5.Role of Postoperative Multimodal Analgesia in Abdominal and Pelvic Enhanced Recovery after Surgery.
Acta Academiae Medicinae Sinicae 2016;38(4):458-463
Enhanced recovery after surgery (ERAS) is to achieve early recovery for patients undergoing major surgery through multimodal perioperative care pathways. Treatment of postoperative pain is of great importance for ERAS. From 2012 to now,the ERAS Society has published seven international guidelines for the abdominal or pelvic surgeries. Each of these guidelines recommended a standardized postoperative multimodal analgesia protocol to improve pain relief and postoperative recovery. Upon these guidelines,thoracic epidural analgesia should be the primary choice for postoperative analgesia of either abdominal and pelvic surgeries. Meanwhile,postoperative multimodal analgesia should contain multiple methods and drugs. In this review,we ellucidate the recommendations on postoperative multimodal analgesia from these seven ERAS guidelines,so as to facilitate the implementation of standardized postoperative multimodal analgesia.
Abdomen
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surgery
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Analgesia
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methods
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Humans
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Pain, Postoperative
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therapy
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Pelvis
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surgery
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Postoperative Period
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Practice Guidelines as Topic
6.Penile replantation: report of two cases and review of the literature.
Gui-zhong LI ; Feng HE ; Guang-ling HUANG ; Li-bo MAN ; Kun LIU ; Yu-ming SHEN
Chinese Journal of Traumatology 2013;16(1):54-57
Penile amputation and successful replantation is very uncommon, and there is no routine standardized procedures for dealing with this medical condition. Here we report two cases of penile amputation and replantation involving different degrees of vascular insult leading to different pathogenesis, clinical presentation, surgical approach and prognosis. This report described the microsurgical procedure and postoperative care using bipedicled scrotal flap to achieve successful engraftment and function. A review of the published data and future methods to increase success of such surgical procedures is provided.
Adult
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Amputation, Traumatic
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surgery
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Humans
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Male
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Microsurgery
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Penis
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blood supply
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injuries
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surgery
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Replantation
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methods
7.Surgical treatment for patients with primary hyperparathyroidism.
Shen-ming WANG ; Xiao-xi LI ; Guang-qi CHANG ; Jin-song WANG ; Xue-ling HUANG
Chinese Journal of Surgery 2004;42(9):532-535
OBJECTIVETo evaluate the feasibility and efficacy of surgical treatment for patients with primary hyperparathyroidism (pHPT).
METHODSThe studies were analyzed for 55 patients with pHPT who underwent parathyroidectomy in our hospital from 1985 to 2002.
RESULTSEight patients were asymptomatic. The skeletal manifestations were found in 24 cases, urinary stones in 10 cases, and both skeletal manifestations and urinary stones in 13 cases. Pathological bone fractures occurred in 16 cases. Hypercalcemia was discovered in all patients with the average value of (3.1 +/- 0.4) mmol/L, ranging from 2.7 to 3.9 mmol/L and. Fifty patients showed elevated parathyroid hormone (PTH) with the average value of (489.2 +/- 69.2) pg/ml, ranging from 102 to 2,000 pg/ml. Preoperatively all patients underwent sonography, CT and/or scintigraphy. The overall preoperative image-directed localization rate was 90.9%. Follow-up was done from 6 months to 2 years after surgery. The symptoms and signs of all patients relieved postoperatively with the improving of osteoporosis and healing of bone fracture. Of all cases, 39 presented with temporary hypocalcemia, 37 showed circumoral paresthesia in whom 10 showed tetany, 15 showed eucalcemia and one had mild hypercalcemia after operation. The serum calcium was normal in all cases with hypocalcemia by Rocaltrol and calcium supplementation for 1 - 3 weeks. PTH level decreased to normal fro 2 weeks to 2 months in 47 cases and was still mildly higher than normal in 3 cases.
CONCLUSIONSParathyroidectomy is an effective approach to patients with pHPT. With preoperative image-directed localization techniques, minimally invasive parathyroidectomy is a valid surgical strategy.
Adolescent ; Adult ; Aged ; Female ; Humans ; Hyperparathyroidism ; diagnosis ; surgery ; Male ; Middle Aged ; Parathyroidectomy ; methods ; Retrospective Studies ; Treatment Outcome
8.Positional cloning of a novel allele of zebrafish cloche mutant.
Ning MA ; Zhong-jun HUO ; Guang YAN ; Hong-hui HUANG ; Shen-qiu LUO ; Wen-qing ZHANG
Journal of Southern Medical University 2010;30(3):458-462
OBJECTIVETo perform the genetic identification of cloche(172) mutant zebrafish.
METHODSThe chemical mutagen N-ethyl-N-nitrosourea (ENU) was used to treat the AB stain male fish. Large-scale forward genetic screening was carried out to search for lyC-deficient zebrafish mutant by WISH. The morphology changes of the embryos at 3 days postfertilization (3dpf) stage were observed and the cloche(172) gene was identified by mapping and complementation test.
RESULTSWe selected 4 lyC-deficient zebrafish by WISH. cloche(172) mutant showed morphological changes similar to cloche mutant in 3dpf stage. One fourth of the embryos showed cloche phenotype as found in complementation test, and the cloche(172) gene was mapped on the telomere of zebrafish 13 chromosome where cloche gene was located. Numerous red blood cells were observed in the cloche(172) mutant, while only a few cells were found in the cloche mutant in the tail region by o-dianisdine staining.
CONCLUSIONcloche(172) gene which is responsible for the phenotype of cloche mutant may be a novel point mutation allele of the cloche mutant.
Alleles ; Animals ; Chromosome Mapping ; Cloning, Molecular ; Embryo, Nonmammalian ; embryology ; metabolism ; Ethylnitrosourea ; toxicity ; Genetic Complementation Test ; Male ; Muramidase ; genetics ; Mutation ; Zebrafish ; embryology ; genetics ; Zebrafish Proteins ; genetics
9.Cardiovascular Disease Risk Factors in Adolescents with Family History of Hypertension
Bo-Wei LIU ; Fu-Zai YIN ; Chun-Ming MA ; Qiang LU ; Dong-Hui LOU ; Rui WANG ; Gai-Ling HUANG ; Guang-Fei WU ; Yi SHEN ; Chunmei QIN ; Bo LIU ;
Chinese Journal of Hypertension 2007;0(05):-
Objective To explore the risk factors of cardiovascular disease in adolescents with a family history of hypertension.Methods A cross-sectional study was conducted in 3874 adolescents ages 13-18 years,with normal BP in 3724 people.Based on family history of hypertension (FH),the cohort of adolescents were dichoto- mized as postive family history (FH~+,n=1145) and negative (FH~-,n=2579).Height,weight,waist circum- ference,hip circumference,blood pressure and fasting plasma glucose(FPG),total cholesterol(TC),triglyceride (TG) and high-density lipoprotein cholesterol(HDL-C) were determined.Results FH~+ adolescents had signifi- cantly higher levels of body mass index(BMI),waist circumference,WHR,FPG,TC and LDL-C(P
10.A Rapid and Simple Method for Cloning and Identification of cDNAs Related with IL-6 Effect
Yao-Jiang HUANG ; Yan LI ; Yue LI ; Guang-Tao SHEN ; Lin-Xia ZHANG ; Jin-Chao FENG
Progress in Modern Biomedicine 2007;7(4):529-532
Cloning of differentially expressed genes is one of the hottest topics in biology.It is very important in cloning genes correlated with phenotype and diseases at molecular level.Here we utilized a simple and rapid PCR-based protocol to detect and isolate cDNA fragments from differentially expressed genes of IL-6-induccd and IL-6-uninduced U937 cells in tWO easy steps.To generate cDNAs from most mRNAs,the first step was reverse transcription using three fully degenerated 6-mer oligonucleotides as primers.The second step was PGR amplification of internal regions of the cDNAs with two or three longer primers with arbitrary but defined sequences.The PCR amplification was repeated on the same cDNA templates(first step) with different sets of primers.DNA fragments were easily displayed by 2% agarose gel electrophoresis and then the differential recovered fragments were used directly in cloning,sequencing,and RNA reverse Northern blot analysis.In this study,seven differential ESTs are obtained;two Sequences not found in GenBank,are novel ESTs.They were proved to be differentially expressed genes related with IL-6 effect by reverse Northern hybridization.