Objective To assess the safety and efficacy of the transohturator tape (TOT) for surgical treatment of female stress urinary incontinence (SUI). Methods Clinical data of 206 TOT procedures for female urinary incontinence from Jan 2007 to Dec 2009 was retrospectively analyzed.Thirty-one women were identified as having mixed stress urinary incontinence.The mean age was 54.29 years(range,32-81 years),themean disease coursewas 10.31 years (range,1-40 years),and the mean follow-up time was 17.3 months (range,3-39 months).Therapeutic effects were assessed by Grouts-Blaivas outcome score.The results of the operation,complications and changes of urodynarnics were observed. Results The TOT operations were conducted under local anesthesia.The mean operative time was 23.9 min (range,16-35 min),Mean blood loss was 17.8 ml(range,15-30 ml).Short-term complications were as follows:thigh pain 14.08%,dysuria 5.83%,urinary posture change 0.97% and urinary retention 0.49%.The long-term complication rate was 0.97%,including vaginal wall defect and tape exposure in one case:the burr of tape exposure and sex life influence in one case.Long-term symptoms disappeared completely in 202 cases.The cure rate was 98.06%,and the rate of satisfaction was 100%. Conclusions Our results demonstrate that TOT procedure is a minimally invasive,safe and effective surgical treatment for stress urinary incontinence.

Adult ; Aged ; Aged, 80 and over ; Female ; Helicobacter Infections ; metabolism ; Helicobacter pylori ; classification ; pathogenicity ; Humans ; Lymphatic Metastasis ; Male ; Microvessels ; pathology ; Middle Aged ; Neoplasm Invasiveness ; Neovascularization, Pathologic ; microbiology ; pathology ; Stomach Neoplasms ; blood supply ; metabolism ; microbiology ; Vascular Endothelial Growth Factors ; metabolism

Articulation Disorders ; surgery

Objective To analyze the efficacy and safety of suprapubic arc sling (SPARC) and transobturater vaginal tape (TOT) for female stress urinary incontinence (SUI). Methods A retrospective study was conducted on 275 women ( 139 in TOT group and 136 in SPARC group) in our institute from September 2008 to January 2011.We had compared the complications of the two groups.All the patient's ICI-Q-SF scores and PISQ-12 scores were collected.We compared the pre- and post-operative Qmax and PVR to find out if SPARC or TOT tap cause bladder outlet obstruction (BOO). Results The operative time was 25.1 ± 3.3 min for TOT and 34.8 ± 5.6 min for SPARC.There was statistical difference between the two groups in the aspects of operative time (P =0.00).The improvements of average symptom and QOL scores in patients undergoing TOT and SPARC were statistically significant (P =0.00).The improvements of the PISQ-12 scores in the two groups were statistically significant (P ＜0.05 ) as well.There was no significant difference (P ＞ 0.05 ) in the total cure rate of TOT and SPARC.The complications post SPARC and TOT were low.In the TOT group,the Qmax changed from 30.2 ± 8.7 ml/s per-operatively to 24.9 ± 8.6 ml/s post-operatively in a week.PVR changed from 1.6 ± 4.2 ml pre-operatively to 3.2 ± 6.5 ml post-operatively in a week.In the SPARC group,the Qmax changed from 31.7 ±9.4 ml/s pre-operatively to 26.9 ± 8.9 ml/s post-operatively in a week.PVR changed from 1.4 ± 3.7 ml pre-operatively to 3.5 ± 6.3 ml postoperatively in a week.All the changes were significant ( P ＜ 0.05 ).And in the SPARC group,the Qmax changed from 31.2 ± 10.0 ml/s pre-operatively to 26.2 ± 9.1 ml/s post-operatively; the PVR changed from 1.8 ± 3.2 ml pre-operatively to 4.7 ± 8.8 ml post-operatively (P ＜ 0.05). Conclusions Both the TOT sling and the SPARC sling have high cure rates for SUI.But the retropubic sling may lead to BOO and other complications,so the transobturate sling is a better treatment option for SUI.

A novel nucleic acid amplification method,termed loop-mediated isothermal amplification(LAMP),which amplifies DNA with high specificity,efficiency,and rapidity under isothermal conditions,may be a valuable tool for the rapid detection of infectious diseases.This method employs a DNA polymerase that have activity of strand displacement DNA synthesis and a set of four specially designed primers that recognize a total of six distinct sequences on the target DNA.LAMP can amplify a few copies of DNA to 109 in less than an hour.The final products are stem-loop DNA with several inverted repeats of the target and cauliflower-like structures with multiple loops.A positive reaction would be shown as a ladder-like pattern in a gel electrophoresis analysis.Because of the advantage,the LAMP method will be widely applied to research of nucleic acid,clinical diagnosis of infectious diseases and detection of genetically modified organisms etc.

Standards of commodity classes of Chinese materia medica is an important way to solve the "Lemons Problem" of traditional Chinese medicine market. Standards of commodity classes are also helpful to rebuild market mechanisms for "high price for good quality". The previous edition of commodity classes standards of Chinese materia medica was made 30 years ago. It is no longer adapted to the market demand. This article researched progress on standards of commodity classes of Chinese materia medica. It considered that biological activity is a better choice than chemical constituents for standards of commodity classes of Chinese materia medica. It is also considered that the key point to set standards of commodity classes is finding the influencing factors between "good quality" and "bad quality". The article also discussed the range of commodity classes of Chinese materia medica, and how to coordinate standards of pharmacopoeia and commodity classes. According to different demands, diversiform standards can be used in commodity classes of Chinese materia medica, but efficacy is considered the most important index of commodity standard. Decoction pieces can be included in standards of commodity classes of Chinese materia medica. The authors also formulated the standards of commodity classes of Notoginseng Radix as an example, and hope this study can make a positive and promotion effect on traditional Chinese medicine market related research.
China ; Drug Industry ; economics ; standards ; Drugs, Chinese Herbal ; economics ; standards ; therapeutic use ; Humans ; Medicine, Chinese Traditional ; economics ; methods ; standards ; Panax notoginseng ; anatomy & histology ; chemistry ; Phytotherapy ; economics ; methods ; standards ; Plant Roots ; anatomy & histology ; chemistry ; Quality Control ; Reference Standards ; Research ; trends

Pharmaceutical market is a typical market with information asymmetry, and which can lead to "lemons" problem. In all developed countries, firms must receive regulatory approval to market a pharmaceutical product. Such administrative department including SFDA, EMA, FDA and so on. Chinese materia medica is a special part of pharmaceutical market in China. The management of Chinese materia medica is a special challenge in China.
Accreditation ; Materia Medica ; economics ; standards ; Medicine, Chinese Traditional ; economics ; standards ; Reference Standards ; Social Control, Formal

OBJECTIVETo observe the relation between Pi deficiency syndrome (PDS) and the configuration and functions of extensor digitorum longus (EDL)and soleus (SOL).

METHODSTotally 36 ICR mice were randomly divided into 3 groups according to weight matching principle, the control group, the exhausted group, and the rhubarb group, 12 in each group. Two PDS models were established by either purgation with rhubarb diarrhea (as Group A) or exhausted swimming plus sleep deprivation (as Group B).The cross sectional area (CSA) of type I and II fibers of extensor digitorum longus (EDL) and soleus (SOL), relative proportions of type I and II fibers were measured by m-ATPase histochemical method. The isotonic contraction and the maximum tetanus contraction of EDL and SOL were detected by PowerLab system.

RESULTSCompared with the control group, the body weight, body temperature, and the general health condition of PDS model rats obviously decreased; the spleen index and the thymus index were also lower; the maximal isotonic contraction and the maximum tetanus contraction obviously decreased; the cross section areas of EDL and SOL were reduced with loosely arranged cells. In EDL, the proportion of type I fibers was added and the proportion of type II fibers was lowered. In SOL, there was no change in the proportion of type I and type II fibers.

CONCLUSIONSEDL and SOL were obviously atrophied in the two PDS model mice. The type I fibers of SOL was more significantly atrophied in Group B.

Animals ; Disease Models, Animal ; Medicine, Chinese Traditional ; Mice ; Mice, Inbred ICR ; Muscle, Skeletal ; physiopathology ; Rats

AIM: To study the causes of pyogenic granuloma after hydroxyapatite(HA) orbital implants.METHODS: HA orbital implants (250 cases) in our hospital (68 pegged implants) were reviewed.All patients were followed up from 18 months to 10 years. Implants were removed after medical therapy which was proved to be ineffective.RESULTS: Ten of 250 cases of HA orbital implants developed pyogenic granuloma. Pyogenic granuloma occurred in 1 unpegged implants patient and 9 patients after pegging and drilling of HA implantation over 4～7 years. The pyogenic granulomas were not controlled by medical therapy effectively. Implants were removed in 9 cases except 1 case denied removing and continued medical therapy.CONCLUSION: Pyogenic granuloma was serious complication that occurred after HA orbital implants. Partial vascularization, implant exposure, xenogenic sclera implant, pegging and drilling of HA implantation are risk factors that affect the development of pyogenic granuloma.Pyogenic granuloma hasn't relation with implanted peg material. Pyogenic granuloma denotes the potential implant infection, and all implants should be removed finally.

OBJECTIVETo observe the effects of acupuncture at "Tianshu" (ST 25) on electro-activity and mechanical motility at different phases of migrating motor complex (MMC) during jejunal digestion period in rats with detached jejunum, so as to explore the effect and mechanism of acupuncture on regulating intestinal movement.

METHODSSixteen adult SD rats were selected. Electrodes were implanted in the serous membrane of intestinal smooth muscl.e and high-sensitivity sensors of strain gauge were sutured on serosal surface, and then the rat was anesthetized and its jejunum was detached. Electro-acriviry and mechanical motility of jejunal smooth muscle were recorded simultaneously. Acupuncture was applied at "Tianshu" (ST 25) at MMC I , MMCII and MMC III, respectively, to observe its influence on electro-activity and mechanical motility.

RESULTSAt phase of MMC I, there was no obvious change of the fast wave before and after the acupuncture, while the frequency and amplitude of slow wave and mechanical motility were both significantly decreased compared with baseline (P < 0.01). At MMCII-Ill, the frequency and amplitude of fast wave, slow wave and motility were all significantly decreased compared with baseline (P < 0.01). Acupuncture at "Tianshu" (ST 25) had prohibited effects on electro-activity and mechanical motility of jejunal smooth muscle in rats with detached jejunum.

CONCLUSIONAcupuncture at "Tianshu" (ST 25) has obvious prohibited effects on electro-acrivity and mechanical motility at MMC I , MMC II and MMC III time phases in rats with detached jejunum. The possible mechanism is that acupuncture at "Tianshu" (ST 25) could prohibit jejunum movement through reflex path of skin-sympathetic.

Acupuncture Points ; Acupuncture Therapy ; Animals ; Digestion ; Electrophysiological Phenomena ; Gastrointestinal Diseases ; physiopathology ; therapy ; Humans ; Jejunum ; chemistry ; physiology ; Male ; Myoelectric Complex, Migrating ; Rats ; Rats, Sprague-Dawley