1.The change of glomerular pathology in children with steroid resistant nephropathy.
Guang-hua ZHU ; Yun-jiu LUO ; Wei-xun HE ; Ming-jun FANG
Chinese Journal of Pediatrics 2004;42(3):218-219
Antibodies
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analysis
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Biopsy
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Child
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Child, Preschool
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Disease Progression
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Drug Resistance
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Female
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Fluorescent Antibody Technique
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Humans
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Immunoglobulin A
;
analysis
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Kidney Glomerulus
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immunology
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pathology
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Male
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Nephrosis
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drug therapy
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pathology
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Prognosis
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Steroids
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pharmacology
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therapeutic use
2.Relationship between Spondyloppiphyseal Dysplasia Tarda Gene Escaping X Chromosome Inactivation and Spondyloppiphyseal Dysplasia Tarda Phenotype
chao, GAO ; huai-li, WANG ; qiang, LUO ; guang-yao, SHENG ; jian-hua, ZHOU ; tie-zheng, GAO
Journal of Applied Clinical Pediatrics 2003;0(10):-
Objective To explore the relationship between X - linked spondyloepiphyseal dysplasia tarda (SEDL) gene escaping X chromosome inactivation( XCI) and SEDL phenotype. Methods RT - PCR was performed on total RNA which was isolated from blood samples of patients, female carriers and controls. Patients and female carriers were selected from the pedigree with SEDL caused by the mutation (IVS2 - 2A→C) of the gene. cDNA was analyzed by polyacrylamide gelelectrophoresis(PAGE). Results PAGE data indicateed that female carriers expressed both normal and mutant SEDL mRNA,meaning the SEDL gene escaping XCI. Family investigation showed carrier females in the SEDL pedigree presented no symptoms. Conclusions The SEDL gene escaping X chromosome in-activation is firstly identified from human body. This may explain that carrier females present no symptoms.
3.Effect of Melatonin on CD4~+CD_(25)~+ Regulatory T Cell and Airway Inflammation in Asthmatic Rat
min, WANG ; jun-lan, YAO ; an-xiu, XIONG ; guang-huan, ZHANG ; qun-xing, WANG ; chun-hua, LUO
Journal of Applied Clinical Pediatrics 1994;0(04):-
Objective To explore the effect of Melatonin(MT) on CD4+CD25+ regulatory T cell (CD4+CD25+Tr)and airway inflammation in asthmatic rat.Methods Thirty-two SD rats were randomly divided into 4 groups,8 rats in each group.Asthmatic group:rats were immunized on day 1 and 7 by intraperitoneal inject of mixture of ovalbumin(OVA) and aluminumhydroxide.From day 14,the animals were allenged with aerosolized OVA for 20 min per day for 7 consecutive days.MT group:OVA-sensitized rats were injected intraperitoneally with 0.1 mg/kg MT 30 min before each OVA challenge.Dexamethasone group:OVA-sensitized rats were injected intraperitoneally with 0.5 mg/kg Dexamethasone 30 min before each OVA challenge.Control group:OVA for inhalation and MT for intraperitoneal injection was replaced with saline.After the last challenge,peripheral blood was stained to count the percentage of eosinophil(EOS).Then the rats were lavaged and total leukocytes counts in bronchoalveolar lavage fluid(BALF) were performed after staining with Wright-Giemsa staining.The EOS counts around the airway was counted after the histological section of lung staining with hematoxylin and eosin staining.The serum level of immunoglobulin E(IgE) was detected by immunoenhancement.The change of CD4+CD25+Tr was assessed with flow cytometry.SPSS 10.0 software was applied to analyze data. Results In asthmatic rats,the CD4+CD25+ Tr/ CD4+T cells ratio had significant negative relationship with the EOS counts around the airway and the total leukocytes counts in BALF (r=-0.73 P0.05).There was a significant decrease in the percentage of the eosinophils in peripheral blood,the eosinophil counts around the airway,the total leukocytes counts in BALF and the serum level of IgE in MT group compared with asthmatic group (Pa
4.Randomized Clinical Trial of Chemotherapy Combined with Radiotherapy for Advanced Nasopharyngeal Carcinoma
Mei-Hua LUO ; Qiang QIN ; E-Luo WEN ; Shi-Rong GAO ; Luo PAN ; Guang-Wei YANG ; Jie CHEN
Chinese Journal of Cancer 2001;20(4):409-411
Objective: The aim of this study was to evaluate the effect of chemotherapy combined with radiotherapy for the treatment of the patients with advanced nasopharyngeal carcinoma. Methods: Between June,1992 and January,1995, 110 patients with Stage Ⅲ , Ⅳ a nasopharyngeal carcinoma ( 92 stage system) at random received one course of chemotherapy [cisplatin 20 mg· (m2· d)-1 iv on days 1-5 and 5-fluorouracil 500 mg· (m2· d)-1 iv on days 1-5.] before radiotherapy (which started 3 days after the first cycle of chemotherapy ) and three to five cycles of postradiotherapy chemotherapy (chemotherapy/radiotherapy: including 55 patients) or radiotherapy alone (radiotherapy group:including 55 patients). The methods and time/dose schedule of radiotherapy were similar in the two groups. Results: The 5-year survival rate was 65.5% in the chemotherapy/radiotherapy as compared with 34.5% in the radiotherapy group (P=0.0012). The primary lesion control rates were 69.1% (chemotherapy/radiotherapy) and 43.6% (radiotherapy group), respectively(P=0.0071). The cervical lymph node control rates were 67.3% (chemotherapy/radiotherapy) and 45.4% (radiotherapy group), respectively (P=0.021). The distant metastatic rate was 21.8% (chemotherapy/radiotherapy) versus 41.8% (radiotherapy group), respectively (P=0.0243). There was no significant difference in the incidence and severity of acute mucositis between the two groups during radiotherapy. Conclusion:This prospective study has demonstrated that alternating cisplatin/5-fluorouracil chemotherapy and early administration of locoregional radiotherapy can improve the survival and local disease control , and delay the distant metastasis in the patients with advanced nasopharyngeal carcinoma.
6.A case report of infantile myofibromatosis of left mandibular angle.
Hua-hua SHUI ; Shang-zheng LIANG ; Ling LUO ; Wei ZHAO ; Guang-xin FU ; Jia HU ; Tao JIANG
West China Journal of Stomatology 2008;26(3):340-341
The clinical data of one case of infantile myofibromatosis of left mandibular angle were analyzed, and the clinicopathological characteristics, imaging diagnosis, treatment and prognosis of infantile myofibromatosis were discussed.
Humans
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Myofibromatosis
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congenital
7.Role of phospholipase C-gamma1 signaling pathway in H(2)O(2)-induced apoptosis of PC12 cells.
Wen-Li YUAN ; Di LU ; Jun SUN ; Guang-Xue CHEN ; Hui CHEN ; Ting-Hua WANG ; Shen-Qiu LUO
Journal of Southern Medical University 2008;28(11):1939-1941
OBJECTIVETo explore the role of phospholipase C-gamma1 (PLC-gamma1) signaling pathway in H(2)O(2)-induced apoptosis of PC12 cells.
METHODSPC12 cells were exposed to 50 micromol/L H(2)O(2) after pretreatment with 10 micromol/L U73122, a specific PLC-gamma1 inhibitor. Hoechst/PI double staining was performed to observe the morphological changes of the cells under light microscope. MTT assay was used to evaluate the cell viability, and the percentage of apoptotic cells was analyzed by flow cytometry. DNA fragmentation assay was carried out to characterize the cell apoptosis.
RESULTSAfter inhibition of the PLC-gamma1 signaling pathway with 10 micromol/L U73122, PC12 cells showed obvious apoptotic morphology, the viable cells decreased significantly, and the percentage of apoptotic cells rose to 35.7%. PC12 cells treated with U73122 presented with a distinct DNA ladder on electrophoresis resulting from DNA cleavage in the apoptotic cells.
CONCLUSIONPLC-gamma1 signaling pathway plays an important protective role in H(2)O(2)-induced PC12 cell apoptosis.
Animals ; Apoptosis ; drug effects ; Estrenes ; pharmacology ; Hydrogen Peroxide ; pharmacology ; PC12 Cells ; Phospholipase C gamma ; antagonists & inhibitors ; metabolism ; Pyrrolidinones ; pharmacology ; Rats ; Signal Transduction
8.Prospective study of early application of immune-enhanced enteral nutrition and recombined human growth hormone on patients with gastric neoplasms after total gastrectomy.
Bei LU ; Yang CAI ; Guang-Hua FENG ; Zhong-Yao LUO ; Wei ZHU ; Jie NI ; Xi-Ping ZHANG
Chinese Journal of Gastrointestinal Surgery 2007;10(6):550-554
OBJECTIVETo explore the effect of immune-enhanced enteral nutrition (IEN) together with recombined human growth hormone (rhGH) on patients after total gastrectomy.
METHODSForty-eight patients after total gastrectomy were randomly divided into EN group (n=16), IEN group (n=16) and IEN+ rhGH(n=16) group. Nitrogen balance, nutritional status, immune function and lassitude degree were compared among 3 groups.
RESULTSIEN+rhGH group had better efficacy as compared to EN and IEN group in improving postoperative nutritional status, immune function, nitrogen balance and lassitude degree, and recovered to normal level after 7 days. All the indexes of IEN+rhGH group except CD8 were improved significantly on the 10th day after operation as compared to those of EN group[total protein(66.8 +/- 2.0)g/L vs (65.8 +/- 0.9)g/L, CD3(66.1 +/- 6.3)% vs (60.5 +/- 5.6)%, Christensen score (4.6 +/- 0.9) vs (6.3 +/- 0.9), all P<0.05].
CONCLUSIONEarly application of IEN combined with rhGH plays an effective role in improving protein metabolism and immune function for patients after total gastrectomy in short period.
Aged ; Enteral Nutrition ; methods ; Fat Emulsions, Intravenous ; Female ; Gastrectomy ; Human Growth Hormone ; immunology ; therapeutic use ; Humans ; Male ; Middle Aged ; Postoperative Period ; Prospective Studies ; Recombinant Proteins ; immunology ; therapeutic use ; Stomach Neoplasms ; therapy
9.Biological significance of relationship between nuclear localization of Rac1 and progression of gastric carcinoma.
Long SU ; Wei XU ; Guang-zhi YAN ; Wei LUO ; Ying-li WANG ; Shuang CHEN ; Yang ZHANG ; Li-hua LIU
Chinese Journal of Oncology 2011;33(9):676-680
OBJECTIVETo observe the subcellular localization of Rac1 and the expression of Tiam1 and Rac1 in gastric carcinoma, in order to reveal the relationship between the distribution of Rac1 and progression of gastric carcinoma.
METHODSBoth carcinoma and adjacent normal tissue of 48 patients with gastric carcinoma were studied in this study. Tissue distribution and expression of Rac1 and Tiam1 were analyzed by immunohistochemistry and real-time polymerase chain reaction (PCR).
RESULTSCompared with that of adjacent non-cancerous gastric mucosa, the expression of Rac1 in cancer tissues was significantly increased. The positive rate of Rac1 expression was 18.8% (9/48 cases) in adjacent non-neoplastic gastric and 79.2% (38/48 cases) in cancer tissues. The positive staining was mainly located in the cell nuclei (31 samples). The real-time PCR results demonstrated that the expression levels of Tiam1 and Rac1 mRNA in cancerous tissues with nuclear localization of Rac1 were evidently increased. Furthermore, nuclear localization of Rac1 was associated with tumor stage and metastasis.
CONCLUSIONSThe majority of gastric cancer tissues show nuclear dislocalization of Rac1 expression, which may be a sign of abnormal activation of Tiam1-Rac1 pathway. It may suggest enhanced invasion ability of the gastric carcinoma.
Adult ; Aged ; Aged, 80 and over ; Cell Nucleus ; metabolism ; Disease Progression ; Female ; Guanine Nucleotide Exchange Factors ; genetics ; metabolism ; Humans ; Immunohistochemistry ; Lymphatic Metastasis ; Male ; Middle Aged ; Neoplasm Invasiveness ; Neoplasm Metastasis ; Neoplasm Staging ; Polymerase Chain Reaction ; RNA, Messenger ; metabolism ; Stomach Neoplasms ; metabolism ; pathology ; T-Lymphoma Invasion and Metastasis-inducing Protein 1 ; rac1 GTP-Binding Protein ; genetics ; metabolism
10.Aberrance analysis of mitochondrial DNA in a family with hereditary ataxia in Guangxi province.
Jing WANG ; Hui-hua LIU ; Shu-guang LUO
Chinese Journal of Medical Genetics 2006;23(3):323-325
OBJECTIVETo investigate the point mutations of mitochondrial DNA in the families with hereditary ataxia.
METHODSPolymerase chain reaction and single strand conformation polymorphism (SSCP) were used to analyze the mitochondrial DNA extracted from human peripheral white blood cells from the families with HA and 35 normal controls. Sequencing was performed to search the point mutations in mitochondrial DNA of those subjects whose results of SSCP were abnormal.
RESULTSA mitochondrial DNA point mutation 11893(A>G) was identified in 2 patients and 1 family member without symptoms.
CONCLUSIONA new point mutation 11893(A>G) of detected mitochondrial DNA may be relative to hereditary ataxia.
Aged ; Base Sequence ; China ; DNA Mutational Analysis ; DNA, Mitochondrial ; chemistry ; genetics ; Family Health ; Female ; Humans ; Male ; Middle Aged ; Pedigree ; Point Mutation ; Polymerase Chain Reaction ; Polymorphism, Single-Stranded Conformational ; Spinocerebellar Degenerations ; genetics