Eighty patients with acute exacerbation of chronic obstructive pulmonary disease(COPD)complicated by pulmonary artery hypertention(PAH)were recruited in the study. All patients took conventional western medicine, among them 40 also received injection of Chinese herb medicine Shenxiong(study group)for 14 days. Mean pulmonary artery pressure(mPAP), plasma platelet alphagranule membrane protein(GMP-140), thromboxane B2(TXB2)and 6-keto-prostaglandin F1α(6-K-PGF1α)were measured before and after treatment in both groups. Compared with pretreatment levels and levels in control group mPAP, GMP-140 and TXB2 levels in study group were significantly decreased, while the levels of 6-K-PGF1α were significantly increased. The results indicate that Shenxiong injection may inhibit platelet activation and reduce mPAP.

Serial cryostat sections of the celiac ganglion were prepared from 30 guinea pigs.The process of De Da Torre's glyoxylic acid method and different histochmical rea-ctions were performed consecutively on the same section.Under the conditions employ-ed in this experiment,it was shown that the SIF cells,in contrast to the principalneurons,exhibited a more intense reation of adenosine triphosphatase,and a lessintense reaction of monoamine oxidase,lactic dehydrogenase and nonspecific esterase,and a negative reaction of alkaline phosphatase and acetylcholinesterase.The acidphosphatase reaction of most of SIF cells was weak as demonstrated with both theGomori's lead method and the simultaneously coupling method.The SIF cells showedless lipids,nucleic acids and about the same content in tyrosin as compared with theprincipal neurons.These histochemical properties of SIF cells,as well as their morphological charac-teristics of grouping in clusters and surrounding blood vessels,implicated that theyfunction in a different way from that of principal neurons,and probably as endocrineand/or paracrine cells in modulating nervous transmission in the celiac ganglion.

Stereological methods were applied in the qantitative ultrastructural analysis of the chromaffin cells in adrenal medulla of normal adult guinea pigs.The main result includes:(1)The average volume(V)of each cell(714?m~3)and its nucleus (167?m~3);(2)volume density(Vv)of mitochondria(0.082?m~3/?m~3),lysosomes (0.0045?m~3/?m~3),rough-endoplasmic reticulium(0.013?m~3/?m~3),smooth-endopas- mic reticulium including Golgi apparatus(0.024?m~3/?m~3)and granule vesicles (0.23?m~3/?m~3);(3)surface density(Sv)of cell membrane(0.87/?m~2/?m~3)and mitochondrial outer membrane(0.90?m~2/?m~3);(4)numerical density(Nv)of mitochondria(0.89/?m~3),lysosomes(0.11/?m~3)and granule vesicles(59.98/?m~3); (5)the mean diameter of granule vesicles(144 nm),In addition,several small- granule chromaffin cells were quantified separately from the general chromaffin cells.They contain granule vesicles with an average diameter of 97 nm and show a significant difference in surface density of cell membrane(1.54?m~2/?m~3)from that relevant value of general chromaffin cells(P

Plasma levels of homocysteine (Hcy),folic acid (FA) and Vitamin B12 (VB12) were measured in 120 patients of colon adenomatous polyps and 40 healthy subjects.Plasma Hcy levels in colon adenomatous polyps patients were significantly higher than those in controls[(14.24 ± 0.52) μmol/L vs.(10.93 ± 0.81) μmol/L,P =0.0014)],while plasma FA levels in polyps patients were lower than those in controls [(6.26 ± 0.27) μg/L vs.(10.74 ± 0.85) μg/L,P ＜ 0.0001].There was no difference in plasma VB12 levels between two groups(P =0.7100).The plasma Hcy levels in patients with multiple colon polyps were higher than those with single colon polyps (P =0.0092),while the plasma FA levels demonstrated contrary results (P =0.0047).With the pathological type progression,the plasma Hcy levels were gradually raised,and FA levels gradually decreased.Plasma levels of FA,Hcy and VB12 were not correlated with the location and distribution of polyps.These results suggest that plasma FA and Hcy levels may be related to disease progress in patients with colon adenomatous polyps.

Objective To determine the levels of radiation-induced expressions of HIF-1α and Survivin in three human lymphoma cell lines with or without HIF-1a specific antagonist or HIF-1a siRNA transfection and to explore the possible mechanism of cell radioresistance.Methods Namalwa,Ramos,Raji cells were irradiated with X-rays with or without the combination of echinomycin (EC),a HIF-1α specific inhibitor,or transfection of HIF-1α siRNA.Then the expression levels of HIF-1α and Survivin proteins and mRNAs were determined by Western blot and real-time PCR,respectively.Results After irradiation,the expression level of HIF-1α protein was up-regulated at 10-20 h followed by up-regulation of survivin protein at 24 h (t =7.53-31.31,P ＜ 0.01 ) in three lymphoma cell lines.Radiation-induced up-expression of survivin protein was significantly reduced by either HIF-1α inhibition or EC treatment (t =7.21-32.81,P ＜0.01 ).However,radiation failed to induce up-erpression of survivin at both mRNA and protein levels after transfection with HIF-1α siRNA.Conclusion HIF-1α-Survivin pathway is activated in human lymphoma cells after radiation,which might be involved in the cell radioresistance.

Objective To evaluate the expression of immunohistochemistry markers p53, p16 and Ki-67 in penile cancer and penile intraepithelial neoplasia (PIN). Methods 33 patients who suffered penile cancer and received organ-sparing surgery from Jan 2004 to Dec 2013 were retrospectively analysed. The expression of p53, p16 and Ki-67 were detected by EnVision/HRP two steps immunohistochemistry. Results The positive expression rates of p53 in normal penile epithelium, low-grate penile intraepithelial neoplasia, high-grate penile intraepithelial neoplasia, well-differentiated squamous cell carcinoma and poor-differentiated squamous cell carcinoma were 0, 16.7 %, 38.5 %, 30.0 % and 66.7 % respectively. The expression rates of p16 were 20.0 %, 50.0 %, 53.8 %, 70.0 % and 33.3 % respectively. The expression rates of Ki-67 were 0, 16.7 %, 61.5 %, 65.0 % and 75.0 % respectively. Conclusions The combined detection of p16 and Ki-67 can contribute to discover PIN and penile carcinoma much more earlier. p53 may be related to the occurrence and development of low differentiated squamous cell carcinoma, and p16 may be related to the occurrence and development of well-differentiated squamous cell carcinoma.

That provincial hospital hosting the local hospital is an important measure to deepening the reform of medical and health system.Advanced hospital management mechanism and excellent technical experts were gradually introduced into the local hospital to establish South Hubei healthcare alliance,and form province-city-county healthcare alliance.In this way,high quality management,manpower and technology resource sink,overall medical technology level achieves good breakthrough.This promotes the rapid development of healthcare alliance member hospitals in the region.

Objective To investigate the distribution of authors,funding,institutions and regions of Chinese Journal of Orthopaedics between 2010 and 2015,understand the types and characteristics of authors＇ cooperation,identify the core author,core institutions and core regions,as well as evaluate academic level and journal quality from the view of literature citation.Methods According to Chinese Science Citation Database (referred CSCD) and bibliometric methods,we made statistical analysis,including the number of papers,cooperation,core authors,regional distribution of authors,systematic distribution of authors,productive institutions,funding sources and distribution.On the basis of Chinese science and technology journal citation reports (Compendex) from 2013 to 2015,the main indicators and coupling relationship of bone surgery journals were evaluated.Results From 2010 to 2015,Chinese Journal of Orthopaedics published 1 342 papers.There were 1 009 first authors,among which 802 authors (79.48％,802/1 009) published only one paper; 1 293 co-author papers contributed to 96.35％ cooperation rate and 5.69 degree of cooperation.Forty-nine papers (3.65％,49/1 342) had only one author,and 799 papers (59.54％,799/1 342) were published by affiliated Hospital,321 papers (23.92％,321/1 342) by provincial and municipal hospitals,138 papers (10.28％,138/1 342) by military hospital.There were 63 (6.24％,63/1 009) core authors publishing 252 papers (18.78％,252/1 342),and 20 productive institution publishing 630 papers (46.94％,630/1 342) in which Beijing was ranked at No.1 with 238 papers (17.73％,238/1 342).From 2010 to 2015,Chinese Journal of Orthopaedics publishing papers got 652 funding.From 2012 to 2014,its core total citations were 10 153,ranked No.1 in bone surgery journals in 2012 and ranked No.2 in 2013-2014; impact factors rose from ranked No.4 in 2012 to ranked No.2 in 2014,and the comprehensive evaluation of total score ranked No.1 in 2012-2014.Conclusion Chinese Journal of Orthopaedics has a strong team with a large number of authors,extensive source of institutions,a wide regional distribution,high cooperation rate,and strong ability to attract assistance funded paper.Affiliated Hospital and provincial and municipal hospitals are the main source of paper in the journal.Its core total citations,impact factor,subject diffusion index,academic impact indicators,comprehensive evaluation score were located in front rank of the same field of journals.

Bone Diseases ; chemically induced ; epidemiology ; China ; epidemiology ; Chronic Disease ; Fluoride Poisoning ; epidemiology ; etiology ; genetics ; Fluorosis, Dental ; epidemiology ; etiology ; Humans ; Prevalence

The celiac ganglia from 9 mature guinea pigs of both sexes were fixed in a mixture of 2％ glutaraldehyde and 2.5％ depolymerized paraformaldehyde. The SIF cells in cryostat sections were discerned out through their eatecholamine fluorescence induced by the fixative. These cells were processed through routine procedures for electron microscopy after or without incubation for acid phosphatase (AcP). The ultrastructure and AcP activity of these cells were observed under an H-600 electron microscope.The SIF cells were found to be present in clusters adjecent to fenestrated capillaries. The exocytosis of vesicular granules of SIF cells were seen on the surface of their cell membrane facing the capillaries. In addition, some dark SIF cells which were more osmiophilic and rich in mitochondria were discovered in this ganglion. These ultrastructural features of the SIF cells indicate that they may perform functions of an endocrine and/or paracrine nature.After one. hour incubation in a Gomori-typed medium at 37℃, some granules. besides the typical lysosomes display the AcP activity. Although the nature of the AcP-positive granules hasn't been elucidated a speculation is laid on that the presence of the enzyme on these granules may play a role of regulation in replacing the intragranular contents.