1.Lacrimal passage probing combining injection of TobraDex eye ointment for treating chronic dacryocystitis
Xiu-Zhang, YANG ; Jun-Hong, FANG ; Guang-Mian, TAN ; Qi-Ling, PENG ; Li, JI
International Eye Science 2006;6(3):550-551
AIM: To evaluate the effect of probing of lacrimal passage combining injecting of US-produced TobraDex eye ointment for the treatment of chronic dacryocystitis.METHODS: For 127 cases (129 eyes) of chronic dacryocystitis, first the pyoid secretion gathered in the lacrimal sac was dashed out, and some TobraDex was injected in the middle of the lacrimal passage once per day, repeated for several times. The lacrimal passage was probed when the secretion of lacrimal sac disappeared essentially. The lacrimal passage and immit TobraDex eye ointment was used once every two days, repeated for 3-4 times.RESULTS: In 126 cases (128 eyes) the lacrimal passage was dredged. Only one case (1 eye) the youthful patient did not recover for the opening ectopia of lacrimonasal duct.During the 3mo-1a random visit the chronic dacryocystitis did not recrudesce in the cases of lacrimal passage dredging.CONCLUSION: It is simple and safe to use the probing of lacrimal passage combining injecting of US-produced TobraDex eye ointment to treat the chronic dacryocystitis. This method has good curative effects and can keep the normal physiological structure of lacrimal passage. It does not need any expensive medical equipment and cost less, therefore is advisable for patients.
2.Testosterone's effect on tPA and PAI-1 mRNA levels of HUVEC.
Hong JIN ; Yu-Guang LI ; Dong-Ming WANG ; Xue-Rui TAN
National Journal of Andrology 2005;11(8):584-586
OBJECTIVETo investigate the effect of testosterone with varied concentrations on the tPA and PAI-1 mRNA levels of Human umbilical vein endothelial cells (HUVEC).
METHODSHUVEC within 2 - 3 passages were cultured with testosterone (3, 30, 3 x 10(3), 3 x 10(4) nmol/L) , and the control confluent cells were cultured in the same medium without steroid for 48 hours. RT-PCR was carried out to detect tPA and PAI-1 mRNA levels.
RESULTStPA mRNA level increased, while PAI-1 mRNA levels decreased significantly, at the testosterone concentrations ranging from 3 to 3 x 10(3) nmol/L (P < 0.05). Both tPA and PAI-1 mRNA level decreased obviously of 3 x 10(4) nmol/L group.
CONCLUSIONThe results indicated that testosterone could stimulate tPA gene expression, while decreased PAI-1 mRNA level of HUVEC, which suggested that testosterone might have beneficial effects on preventing male's thrombotic diseases.
Cells, Cultured ; Dose-Response Relationship, Drug ; Endothelial Cells ; drug effects ; metabolism ; Endothelium, Vascular ; cytology ; metabolism ; Humans ; Male ; Plasminogen Activator Inhibitor 1 ; biosynthesis ; genetics ; RNA, Messenger ; genetics ; Reverse Transcriptase Polymerase Chain Reaction ; Testosterone ; pharmacology ; Tissue Plasminogen Activator ; biosynthesis ; genetics ; Umbilical Veins ; cytology
3.Preliminary study on hepatotoxicity induced by dioscin and its possible mechanism.
Ya-xin ZHANG ; Yu-guang WANG ; Zeng-chun MA ; Xiang-lin TANG ; Qian-de LIANG ; Hong-ling TAN ; Cheng-rong XIAO ; Yong-hong ZHAO ; Yue GAO
China Journal of Chinese Materia Medica 2015;40(14):2748-2752
Dioscin has a wide range of biological effects and broad application prospects. However the studies concerning the toxicology and mechanism of dioscin is small. This article is to study the hepatotoxicity of dioscin and the effect of dioscin treatment on expression of aryl hydrocarbon receptor (AhR) mRNA and CYP1A mRNA and protein in HepG2 cells in vitro. Dioscin 0.5-32 µmol · L(-1) exposed to HepG2 cells for 12 h, cell viability was examined by CCK-8 assay and the release rate of lactate dehydrogenase (LDH) was to evaluate cell membrane damage. HepG2 cells morphologic changes were quantified by inverted Microscope, and the effect on production of reactive oxygen species (ROS) was detected by flow cytometry. The mRNA expression of CYP1A and AhR was evaluated by RT-RCR. The protein expression of CYP1A1 was detected by western blot. The cell viability was significantly inhibited after HepG2 cells were exposed to dioscin 0.5-32 µmol · L(-1). Compared with the control, the LDH release rate and ROS were significantly increased. The expression of CYPlA and AhR mRNA was increased. The expression of CYP1Al protein was increased after dioscin treatment, and resveratrol, an AhR antagonist, could downregulate the expression of CYP1A1. It follows that large doses dioscin has potential hepatotoxicity. The possible mechanism may be dioscin can active aryl hydrocarbon receptor (AhR) and induce the expression of CYP1A.
Cell Survival
;
drug effects
;
Chemical and Drug Induced Liver Injury
;
etiology
;
Cytochrome P-450 CYP1A1
;
genetics
;
Diosgenin
;
analogs & derivatives
;
toxicity
;
Hep G2 Cells
;
Humans
;
L-Lactate Dehydrogenase
;
secretion
;
RNA, Messenger
;
analysis
;
Reactive Oxygen Species
;
metabolism
;
Receptors, Aryl Hydrocarbon
;
genetics
4.Effects of Siwu decoction on bone marrow protein expression of blood deficiency mice induced by cyclophosphamide.
Li-Li LIU ; Zeng-Chun MA ; Yu-Guang WANG ; Hong-Lin QIN ; Hong-Ling TAN ; Cheng-Rong XIAO ; Yue GAO
China Journal of Chinese Materia Medica 2006;31(14):1172-1175
OBJECTIVETo study the effects of Siwu decoction on protein expression of blood deficiency mice induced by cyclophosphamide (CIX) and discuss the possible molecular mechanism on blood enriching function of Siwu decoction.
METHODBlood deficiency mice were established by injecting ip with 250 mg x kg(-1) CTX. Proteomic technologies were applied to identify the different protein.
RESULTSiwu decoction could restore the changes of 12 up-regulated and 3 down-regulated proteins in bone marrow of blood deficiency mice induced by cyclosphosphamide.
CONCLUSIONSiwu decoction could effect expression of proteins which functions including apoptosis, proliferation and differentiation of the haematopoietic stem/progenitor cell. The regulation in the molecular level might be the mechanism of stimulating hematopoiesis in bone marrow fo siwu decocetion.
Actin Depolymerizing Factors ; metabolism ; Anemia ; chemically induced ; metabolism ; pathology ; Animals ; Annexin A1 ; metabolism ; Apoptosis ; drug effects ; Carbonic Anhydrases ; metabolism ; Cell Differentiation ; drug effects ; Cell Proliferation ; drug effects ; Cyclophosphamide ; Drug Combinations ; Drugs, Chinese Herbal ; isolation & purification ; pharmacology ; Fatigue ; chemically induced ; metabolism ; pathology ; Female ; Hematopoietic Stem Cells ; metabolism ; pathology ; Medicine, Chinese Traditional ; Mice ; Mice, Inbred C57BL ; Peroxidases ; metabolism ; Peroxiredoxins ; Plants, Medicinal ; chemistry ; Proteome ; metabolism ; Proteomics ; methods
5.A study on the screening of high-risk population of schistosomiasis using a quick inquiry discriminant method during the years of disasters.
Mei-xia YANG ; Hong-zhuan TAN ; Yi-biao ZHOU ; Guang-ming TANG ; Cong-ya YUN
Chinese Journal of Epidemiology 2003;24(3):199-201
OBJECTIVETo seek a rapid, simple, but effective inquiry method for screening high risk population challenged by schistosomiasis.
METHODSTwo embankment collapsed villages were selected in schistosomiasis epidemic area in Dongting Lake. Information on water exposure was collected through a retrospective study. Data was analyzed by stepwise discriminant analysis.
RESULTSA Fisher's function was established by stepwise discriminant analysis which including 5 variables out of 18. Two hundred and forty-six individuals were discriminated by the function with accuracy, sensitivity and specificity of predicting their current infection status with the results of 87.4%, 84.1% and 89.0% respectively.
CONCLUSIONThe inquiry method might serve as simple, rapid, economic and effective tool for diagnosis in screening high risk population challenged by schistosomiasis in lake communities.
Adult ; China ; epidemiology ; Disasters ; Discriminant Analysis ; Female ; Humans ; Male ; Mass Screening ; methods ; Multivariate Analysis ; Retrospective Studies ; Risk Assessment ; Rural Health ; Sampling Studies ; Schistosomiasis japonica ; epidemiology ; prevention & control ; Sensitivity and Specificity ; Surveys and Questionnaires
6.Role of folate receptor 1 in paclitaxel-resistance of nasopharyngeal carcinoma cells.
Wei LI ; Guo-lin TAN ; Yan-hong MA ; Xiao-wei PENG ; Guang-xiang HE
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2010;45(12):1035-1040
OBJECTIVETo provide experimental evidence for the folate receptor 1 (FOLR1) mediated targeted cancer therapy and resistance reversal, the FOLR1 expression differences in nasopharyngeal carcinoma cells (CNE-1) and immortalized normal nasopharyngeal cells (NP69) and the correlation between FOLR1 expression and paclitaxel resistance index in nasopharyngeal carcinoma were investigated.
METHODSThe expressions of FOLR1 in CNE-1, CNE-1/Taxol (paclitaxel-resistance cells) and NP69 was detected by cDNA microarray, reverse transcriptase-polymerase chain reaction (RT-PCR), Western blot and immunocytochemistry. Proliferation inhibition rates of CNE-1 and CNE-1/Taxol cells were measured by colony formation assay after treated by short interfering RNA (siRNA) of FOLR1.
RESULTSThe expressions of FOLR1 gene in CNE-1/Taxol cells and CNE-1 cells were 2636.0 and 176.0, respectively. The expression of FOLR1 was not detected in the NP69 by semi-quantative RT-PCR and Western blot. The high expression of FOLR1 in CNE-1/Taxol was verified by semi-quantative RT-PCR, and its expression level was positively correlated to the degree of drug-resistance (r(2) = 0.8719). The results were also validated by Western blot and immunocytochemistry. The sensitivity of CNE-1/Taxol to paclitaxel significantly increased after inhibition of FOLR1 gene expression by siRNA, and its IC(50) value was decreased by 59.6% (t = 6.92, P < 0.01).
CONCLUSIONSThe expression of FOLR1 is closely related to the occurrence of NPC and Taxol resistance. FOLR1 gene may be one of the important target molecules in NPC treatment and reversion of the paclitaxel-resistance in NPC.
Cell Line, Tumor ; Drug Resistance, Neoplasm ; Folate Receptor 1 ; genetics ; metabolism ; Humans ; Nasopharyngeal Neoplasms ; drug therapy ; metabolism ; Paclitaxel ; pharmacology
7.Cardiotoxicity study of Shenfu compatibility in rats based on metabonomics.
Jia-le HE ; Jia-wei ZHAO ; Zeng-chun MA ; Qian-de LIANG ; Yu-guang WANG ; Hong-ling TAN ; Cheng-rong XIAO ; Tang XIANG-LIN ; Yue GAO
China Journal of Chinese Materia Medica 2015;40(14):2743-2747
To research the effect of Ginseng Radix et Rhizoma and Aconiti Lateralis Radix Praeparata compatibility on cardiac toxicity in rats by UPLC-Q-TOF/MS, and explore the endogenous markers and molecule mechanism. Different compatibility of Shenfu decoction were given to male Wistar rats at dosage of 20 g · kg(-1) for 7 days, collected the serum, and analyze the endogenous metabolites effected by Shenfu formulation by principal component analysis and partial least-squares analysis. Results showed that content of glutathione, phosphatidylcholine and citric acid decreased in mixed-decoction group, while ascorbic acid, uric acid, D-galactose, tryptophan, L-phenylalanine increased. The results showed cardiac toxicity of Aconiti Lateralis Radix Praeparata in Shenfu mixed-decoction. Shenfu co-decoction group showed a similar or weaker trend compared with control group, but most of them do not have a statistically significant. The results indicated the scientific basis of Shenfu compatibility by comparison of co-decoction group with mixed-decoction group. Shenfu compatibility can reduce cardiac toxicity induced by Aconiti Lateralis Radix Praeparata, and citric acid, glutathione, phosphatidyl choline, uric acid might be regarded as potential markers of cardiotoxicity.
Animals
;
Biomarkers
;
Cardiotoxicity
;
Drugs, Chinese Herbal
;
toxicity
;
Glutathione
;
blood
;
Least-Squares Analysis
;
Male
;
Metabolomics
;
methods
;
Principal Component Analysis
;
Rats
;
Rats, Wistar
8.Effect of Siwu decoction on function and expression of P-glycoprotein in Caco-2 cells.
Yi JIANG ; Zeng-chun MA ; Xian-ju HUANG ; Qing YOU ; Hong-ling TAN ; Yu-guang WANG ; Qian-de LIANG ; Xiang-lin TANG ; Cheng-rong XIAO ; Yue GAO
China Journal of Chinese Materia Medica 2015;40(5):933-937
To study the effect of Siwu decoction on the function and expression of P-glycoprotein (P-gp) in Caco-2 cells. The Real-time quantitative poly-merase chain reaction (Q-PCR) was used to analyze the mRNA expression of MDR1 gene in Caco-2 cells. Flow cytometer was used to study the effect of Siwu decoction on the uptake of Rhodamine 123 in Caco-2 cells, in order to evaluate the efflux function of P-gp. Western blotting method was used to detect the effect of Siwu decoction on the P-gp protein expression of Caco-2 cells. Compared with the blank control group, after Caco-2 incubation with Siwu decoction at concentrations of 3.3, 5.0, 10.0 g x L(-1) for 24, 48, 72 h, the mRNA expression of MDR1 was up-regulated, suggesting the effect of Siwu decoction in inducing the expression of MDR1. After the administration with Siwu decoction in Caco-2 cells for 48 h, the uptake of Rhodamine 123 in Caco-2 cells decreased by respectively 16.6%, 22.1% (P < 0.05) and 45.4% (P < 0.01), indicating that the long-term administration of Siwu decoction can enhance the P-gp efflux function of Caco-2 cells. After the incubation of Caco-2 cells with Siwu decoction for 48 h, the P-gp protein expression on Caco-2 cell emebranes, demonstrating the effect of Siwu decoction in inducing the protein expression of P-gp.
ATP Binding Cassette Transporter, Sub-Family B
;
genetics
;
metabolism
;
ATP-Binding Cassette, Sub-Family B, Member 1
;
genetics
;
metabolism
;
Caco-2 Cells
;
Drugs, Chinese Herbal
;
pharmacology
;
Humans
;
Up-Regulation
;
drug effects
9.Development of sensitive-micro RF coils in desktop MRI systems.
Hong-Zhi WANG ; Zhi-Guang TAN ; Chao-Hui REN ; Cong LIU ; Xue-Long ZHANG
Chinese Journal of Medical Instrumentation 2008;32(1):35-39
A RF coil (or probe) is one of the key components in NMR spectrometers and MRI systems, and designing a quality RF coil is a cheaper way to improves the signal-to-noise ratio (SNR) of systems and image quality. According to different applications there are a variety of RF coils. This article describes high-sensitive micro RF coils used in NMR spectrometers specially for test-tube samples. Their performance has been verified on the desktop MRI systems.
Equipment Design
;
Image Enhancement
;
instrumentation
;
Magnetic Resonance Imaging
;
instrumentation
;
Radio Waves
10.Genetic association of apoE and apoCI gene polymorphisms with coronary heart disease.
Chun-Hong WANG ; Xin ZHOU ; Guang-di ZHOU ; Xiao-dong TAN ; Ding-fen HAN ; Fang ZHENG ; Fang LIU
Chinese Journal of Epidemiology 2004;25(11):982-985
OBJECTIVETo study the genetic association of apolipoprotein (apo) E and apoCI gene polymorphisms with coronary heart disease (CHD) in China.
METHODSapoE genotypes were identified by multiplex amplification refractory mutation system (multi-ARMS) and the apoCI promoter polymorphisms were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in 186 cases with CHD (age: 65.0 +/- 10.5 years) and 350 controls (age: 63.6 +/- 8.3 years). The haplotype frequencies were estimated.
RESULTSThe frequencies of apoE E4/3 genotype (26.9%) and epsilon4 (14.5%) in CHD group were significantly higher than that in the control group (12.6%, 7.0%), P <0.05. The significant difference was also found for the apoCI locus and the CHD group showed higher rate of both for the H2 allele and genotypes, carrying this allele. Estimation of the haplotype frequencies indicated that the association between the apoE-CI haplotype and CHD was significantly strong. The apoE-epsilon4/apoCI-H2 was estimated to be responsible for 9.86% of CHD.
CONCLUSIONWhen the subjects carrying both epsilon4 and H2 alleles, they would have higher risk of suffering from CHD than controls.
Adult ; Aged ; Aged, 80 and over ; Alleles ; Apolipoproteins C ; genetics ; Apolipoproteins E ; genetics ; China ; epidemiology ; Coronary Disease ; blood ; epidemiology ; genetics ; Female ; Genotype ; Haplotypes ; Humans ; Male ; Middle Aged ; Polymerase Chain Reaction ; Polymorphism, Restriction Fragment Length ; Risk Factors