1.Investigation on the antibiotic resistance and risky factors of nosocomial infections caused by Stenotrophomonas maltophilia.
Chao ZHUO ; Yuan-shu QIAN ; Guang-xia XIAO
Chinese Journal of Burns 2004;20(1):10-13
OBJECTIVETo investigate the antibiotic resistance and risky factors of nosocomial infections caused by Stenotrophomonas maltophilia, so as to help elucidate the difference of drug resistance between metallic beta-lactamase (MBL) producing and non-MBL producing strains.
METHODSStandard agar dilution method of NCCLS was employed in the isolation of 36 strains of Stenotrophomonas maltophilia from patients with nosocomial infection with respect to their in vitro antibiotic resistance to 18 kinds of antibiotics. MBL strains were identified by MBL-E test method.
RESULTSStenotrophomonas maltophilia in our hospital was mainly identified in the lower respiratory tract (88.9%), in which 88.2% (30/34) of the patients had serious original diseases, 50% of whom had received Imipenem/cilastatin sodium treatment. Thirty-six strains of Stenotrophomonas maltophilia were susceptible to new types of fluoquinolone antibiotics, i.e. Sparfloxacin, levofloxacin, gatifloxacin and doxycycline, with inhibitory rate ranging 97.2%, 94.4%, 91.7% to 83.3%, respectively. They could also be inhibited by SMZ/TMP and Ticarcillin/Lavulanic acid with inhibitory rate of 63.9% and 58.3%, respectively. There were 16 strains out of 36 of MBL bacteria with complete resistance to Imipenem/cilastatin sodium, but with higher susceptibility to aztreonam than those non-MBL producing strains.
CONCLUSIONThe nosocomial infection in our hospital caused by Stenotrophomonas maltophilia seemed to be related with severe primary disease and the use of Imipenem/cilastatin sodium. The newly developed fluoroquinolones possessed powerful antibacterial potency on Stenotrophomonas maltophilia found in nosocomial infection.
Antibiosis ; drug effects ; Cilastatin ; therapeutic use ; Cross Infection ; drug therapy ; microbiology ; Drug Resistance, Bacterial ; drug effects ; Fluoroquinolones ; therapeutic use ; Humans ; Imipenem ; therapeutic use ; Microbial Sensitivity Tests ; Risk Factors ; Stenotrophomonas maltophilia ; drug effects
2.A preliminary study of beam weight optimization of intensity-modulated radiation therapy with genetic algorithm.
Mu-tao TANG ; Chao-min CHEN ; Ling-hong ZHOU ; Qing-wen LÜ ; Zhuo-yu WANG ; Guang-jie CHEN
Journal of Southern Medical University 2006;26(4):456-458
UNLABELLEDTo study the method for dose calculation and beam weight optimization of intensity-modulated radiation therapy (IMRT).
METHODSThe IMRT dose calculation model based on two-dimensional convolution was constructed, the program of dose calculation and beam weight optimization with genetic algorithm was written with Visual c#.Net, and the optimization results were analyzed.
RESULTSGenetic algorithm optimization of beam weights can produce highly conformal dose distributions within a clinically acceptable computation time.
CONCLUSIONGenetic algorithm is valid and efficient in IMRT beam weight optimization, which may facilitate IMRT treatment planning.
Algorithms ; Humans ; Models, Statistical ; Models, Theoretical ; Radiotherapy Dosage ; Radiotherapy Planning, Computer-Assisted ; methods ; Radiotherapy, Conformal ; methods ; standards
3.Serum IL-18 levels in mice with collagen-induced arthritis treated by recombinant adenovirus containing mIL-18BP and mIL-4 fusion gene.
Jian-Hang LENG ; Hang-Ping YAO ; Jun-Ya SHEN ; Ke-Yi WANG ; Zi-Wei WANG ; Guang-Chao ZHUO
Journal of Zhejiang University. Medical sciences 2011;40(2):195-199
OBJECTIVETo investigate serum IL-18 levels in mice with collagen-induced arthritis treated by recombinant adenoviral vector containing mIL-18BP and mIL-4 fusion gene (AdmIL-18BP/mIL-4).
METHODSArthritis was induced by injection of collagen in male DBA-1/BOM mice. Mice with collagen-induced arthritis (CIA) were intra-articularly injected with 10(7)pfu/6μL of AdmIL-18BP/mIL-4; and in mice of control groups AdLacZ or PBS were used. The animals were sacrificed at week 1, 2 and 4 after treatment. Serum IL-18 levels were determined by ELISA at the different time points.
RESULTThe mean serum levels of IL-18 at weeks 1, 2, and 4 after injection of AdmIL-18BP/mIL-4 were (36.5±5.4)ng/L, (32.5 ± 3.2) ng/L and (28.7 ±2.9)ng/L, respectively, which were significantly lower than those at the same time point of AdLacZ group [(66.2 ±5.1)ng/L, (69.2 ±4.2)ng/L and (77.7 ±3.9)ng/L] and PBS group [(67.3 ±7.1)ng/L, (71.9 ±1.8)ng/L and (78.7±4.1)ng/L] (P<0.01 at all time points). In the therapy group, there were no significant differences in the mean serum concentrations of IL-18 at all time points.
CONCLUSIONThe serum IL-18 levels in CIA mice are down-regulated by treatment of recombinant adenovirus containing mIL-18BP and mIL-4 fuse gene, which might be a promising therapeutic strategy for rheumatoid arthritis.
Adenoviridae ; genetics ; Animals ; Arthritis, Experimental ; blood ; therapy ; Gene Fusion ; Genetic Therapy ; Genetic Vectors ; Interleukin-18 ; blood ; genetics ; Interleukin-4 ; genetics ; Male ; Mice ; Mice, Inbred DBA
4.Relationship Between the Changes of Left Atrium, Pulmonary Vein Ostia and AF Recurrence After Radiofrequency Catheter Ablation by Enhanced Cardiac MRI Evaluation
Xue-Lian LI ; Wen-Su CHEN ; Cheng-Zong LI ; Fei LI ; Chao-Qun ZHANG ; Shu-Guang HAN ; Jia-Li WANG ; Zhi-Rong WANG ; Zhuo-Qi ZHANG
Chinese Circulation Journal 2018;33(4):390-394
Objectives: To evaluate the changes of left atrial volume (LAV) and the maximum ostial cross-sectional area (CAS) of pulmonary vein (PV) in atrial fibrillation (AF) patients after circumferential pulmonary vein isolation radiofrequency catheter ablation (CPVA-RFCA) and to explore their relationship to AF recurrence by enhanced cardiac MRI evaluation. Methods: Our research included in 2 groups: Control group, n=20 healthy subjects and AF group, n=78 patients whom were classified into 2 subgroups as Paroxysmal AF subgroup, n=46 and Persistent AF subgroup, n=32; 66 patients received CPVA-RFCA and based on 6 months post-operative recurrence, they were divided into another set of 2 groups: AF recurrent subgroup, n=17 and Non-AF recurrent subgroup, n=49. Pre- and 6 months post-operative maximum ostial CSA of PV were measured by enhanced cardiac MRI, LAV were obtained by 3D reconstruction and the differences were compared between AF group and Control group, Paroxysmal AF subgroup and Persistent AF subgroup, AF recurrent subgroup and Non-AF recurrent subgroup; their relationships to AF recurrence were studied.Results: Compared with Control group, AF group had increased LAV and elevated ostial CSA of superior PV (SPV), both P<0.05. Compared with Paroxysmal AF subgroup, Persistent AF subgroup had increased LAV and elevated ostial CSA of SPV, both P<0.05. Compared with pre-operative condition, at 6 months after the operation, Non-AF recurrent subgroup showed reduced ostial CSAs in left SPV (LSPV), right SPV (RSPV), right inferior PV (RIPV) and decreased LAV, all P<0.05;while AF recurrent subgroup showed expanded RSPV and increased LAV,allP<0.05.Post-operative reductions of LAV and ostial CSA of SPV had close correlation; multivariate Logistic regression analysis indicated that LAV (HR=1.05, P<0.01)and ostial CSA of RSPV(HR=1.09,P=0.05)were related to AF recurrence after RFCA. Conclusions: CAPV-RFCA could reverse left atrial and PV remodeling in AF patients, LAV and ostial CSA of RSPV were related to post-operative AF recurrence.
5.Clinical Significance of the Application of Thrombelastogram in Perioperative Detection of Esophageal Cancer Patients
Jing-zhuo PANG ; Guo-yong WU ; Min YE ; Wen-guang PANG ; Shi-chao ZHANG ; Cheng-jie CAI
Journal of Sun Yat-sen University(Medical Sciences) 2020;41(6):975-980
【Objective】 Thrombelastogram(TEG) was used to detect the blood clotting function of patients with esophageal cancer in each stage of perioperative period, so as to understand the change pattern of blood clotting function in patients with esophageal cancer during perioperative period, and to guide the postoperative anticoagulation or hemostasis treatment. 【Methods】 Sixty patients diagnosed with esophageal cancer who underwent surgical treatment between May 2017 and May 2019 in Jiangmen Central Hospital were selected as the experimental group, and 60 cases of healthy physical examination were selected as the normal control group. By contrasting with the normal control group, results of blood sample testing for the experimental group 1 day before, 1 day after operation, and the 7th day postoperative thrombosis elastic figure were used to analyze the change of the esophageal perioperative blood coagulation function. 【Results】 Compared with the control group before surgery, the TEG parameters R, K and LY30 decreased, and Angle, MA and CI increased, with statistically significant differences(P < 0.05). Compared with the normal control group after surgery, the R and K values of TEG parameters in the experimental group decreased significantly, while Angle, MA and CI values increased significantly, with statistically significant differences(P < 0.01), and LY30 values decreased, with statistically significant differences(P < 0.05). Compared with the experimental group before and after surgery, the TEG parameters R and K decreased, and the values of LY30, Angle, MA and CI increased, with no statistically significant difference(P > 0.05). Seven days after the operation and 1 day after the operation, the values of R and K of TEG decreased, and the values of MA and CI increased, with statistically significant differences(P < 0.05) . 【Conclusion】 All patients with esophageal cancer presented hypercoagulability before and after operation.Surgery and anesthesia did not significantly change the coagulation status of esophageal cancer. In the postoperative recovery process, the hypercoagulable state will be more obvious.
6.Difference in inclusion behavior of β-cyclodextrin and hydroxypropyl-β-cyclodextrin with menthol.
Guang-Wen WAN ; Hui XIE ; Chun-Qin MAO ; Run-Qu TANG ; Chao YAO ; Zhuo WANG
China Journal of Chinese Materia Medica 2016;41(18):3336-3341
Volatile oils are important active components in traditional Chinese medicine, but their components are complicated and unstable. It is common to use cyclodextrin inclusion technique to improve the stability of volatile oils and make them easier to be prepared. At present, β-cyclodextrin (β-CD) is the most common inclusion material. The evaluation indicators for inclusion technique usually contain the inclusion rate and the oil content in the inclusion compound. However, the articles about the study on selecting inclusion materials for volatile oils were few. In this paper, menthol, the main active ingredient of mint volatile oil, was used as model drug, while β-CD and hydroxypropyl-β-cyclodextrin (HP-β-CD) were used as the inclusion materials. Inclusion equilibrium constant (K), solubilization ratio were investigated, and the results were combined with IR, DSC and TG to verify the formation of inclusion complexes. It turned out that in the range of 0-15 mmol•L⁻¹, the solubility of menthol was increased linearly with the increase of HP-β-CD concentration, with AL-type phase solubility diagram, K=3 188.62 L•mol⁻¹; in the range of 0-12.5 mmol•L⁻¹, the solubility of menthol was increased linearly with the increase of β-CD concentration, K=818.73 L•mol⁻¹. When the concentration was over 12.5 mmol•L⁻¹, the solubility of menthol appeared to be a negative deviation with the increase of β-CD concentration, with AN-type solubility diagram. The above results showed that the inclusion behavior was different between β-CD and HP-β-CD, laying a foundation for further study on inclusion complexes of volatile oil.
7.Application of Keyhole Microneurosurgery in China.
Li-Gang CHEN ; Shu-Da CHEN ; Guang-Fu HUANG ; Ying HUANG ; De-Zhi KANG ; Qing LAN ; Gang LI ; Xin-Gang LI ; Zhi-Xiong LIU ; Song-Tao QI ; Xin-Hua TIAN ; Guo-Liang WANG ; Shuo WANG ; Xiang-Yu WANG ; Yong-Fei WANG ; Yun-Jie WANG ; Chao YOU ; Yan-Bing YU ; Shu-Yuan YUE ; Dong ZHANG ; Jian-Min ZHANG ; Jian-Ning ZHANG ; Jun-Ting ZHANG ; Shi-Zhong ZHANG ; Xian ZHANG ; Ya-Zhuo ZHANG ; Ji-Zong ZHAO ; Wei-Guo ZHAO ; Yuan-Li ZHAO ; Ding-Biao ZHOU ; Liang-Fu ZHOU ; null
Chinese Medical Journal 2017;130(16):1987-1994
8.Expert consensus on the strategy and measures to interrupt the transmission of schistosomiasis in China
Shan LÜ ; Chao LÜ ; Yin-Long LI ; Jing XU ; Qing-Biao HONG ; Jie ZHOU ; Jian-Feng ZHANG ; Li-Yong WEN ; Jian-Feng ZHANG ; Shing-Qing ZHANG ; Dan-Dan LIN ; Jian-Bing LIU ; Guang-Hui REN ; Yi DONG ; Yang LIU ; Kun YANG ; Zhi-Hua JIANG ; Zhuo-Hui DENG ; Yan-Jun JIN ; Han-Guo XIE ; Yi-Biao ZHOU ; Tian-Ping WANG ; Yi-Wen LIU ; Hong-Qing ZHU ; Chun-Li CAO ; Shi-Zhu LI ; Xiao-Nong ZHOU
Chinese Journal of Schistosomiasis Control 2021;33(1):10-14
Since 2015 when the transmission of schistosomiasis was controlled in China, the country has been moving towards elimination of schistosomiasis, with the surveillance-response as the main interventions for schistosomiasis control. During the period of the 13th Five-Year Plan, the transmission of schistosomiasis had been interrupted in four provinces of Sichuan, Jiangsu, Yunnan and Hubei and the prevalence of schistosomiasis has been at the historically lowest level in China. As a consequence, the goal set in The 13th Five-Year National Schistosomiasis Control Program in China is almost achieved. However, there are multiple challenges during the stage moving towards elimination of schistosomiasis in China, including the widespread distribution of intermediate host snails and complicated snail habitats, many types of sources of Schistosoma japonicum infections and difficulty in management of bovines and sheep, unmet requirements for the current schistosomiasis control program with the currently available tools, and vulnerable control achievements. During the 14th Five-Year period, it is crucial to consolidate the schistosomiasis control achievements and gradually solve the above difficulties, and critical to provide the basis for achieving the ultimate goal of elimination of schistosomiasis in China. Based on the past experiences from the national schistosomiasis control program and the challenges for schistosomiasis elimination in China, an expert consensus has been reached pertaining to the objectives, control strategy and measures for The 14th Five-Year National Schistosomiasis Control Program in China, so as to provide insights in to the development of The 14th Five-Year National Schistosomiasis Control Program in China.