2.Effect of Xinnaojia coumfound on learning and memory and expression of NR2B in the hippocampus of rats with chronic alcoholism.
Li SHUANG ; Jia WAN ; Wen-Jie CHEN ; Guang-Rui WAN
Chinese Journal of Applied Physiology 2011;27(1):5-80
Alcoholism
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drug therapy
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metabolism
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physiopathology
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Animals
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Drugs, Chinese Herbal
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pharmacology
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therapeutic use
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Hippocampus
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metabolism
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Learning
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drug effects
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physiology
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Male
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Memory
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drug effects
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physiology
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Phytotherapy
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RNA, Messenger
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genetics
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metabolism
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Rats
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Rats, Sprague-Dawley
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Receptors, N-Methyl-D-Aspartate
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genetics
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metabolism
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Superoxide Dismutase
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metabolism
3.Correlation between immune response to hepatitis B vaccine and HLA DRB1*07 DRB1*04 DRB1*1001.DQB1*0401 genes in Han population in Ningbo
Jin-Feng ZHAO ; Hai-Tang CHEN ; Guang-Wen CAO ;
Academic Journal of Second Military Medical University 2000;0(08):-
Objective:To study the correlation between responses to Hepatitis B and HLA DRB1*07 DRB1*04 DRB1*1001. DQB1*0401 genes in Han population in Ningbo.Methods:A total of 240 Han people living in Ningbo received the routine vaccination of recombinant hepatitis B vaccine.The serum levels of anti-HBs antibody were examined and the subjects were divided into 2 groups according to tile results:negative responses group(n=120.anti-HBs D
4.Microsatellite instability and loss of heterozygosity on chromosome 3p,9p and 14q in renal cell carcinoma
Hai-Tang CHEN ; Wen-Jun CHANG ; Hong-Yu YU ; Jin-Feng ZHAO ; Guang-Wen CAO ;
Academic Journal of Second Military Medical University 1985;0(06):-
Objective:To investigate frequencies of microsatellite instability(MSI)and loss of heterozygosity(LOH)in renal ceil carcinoma(RCC),and to discuss the relationship of clinicopathological characteristics of RCC with MSI and LOH. Methods:Twelve microsatellite markers located at chromosomes 3p,9p and 14q were selected to investigate microsatellite alterations(MSI and LOH)in 31 RCC specimens and their paired metastasis specimens by polymerase chain reaction- polyacrylamide gel elect rophoresis-ethylene dibromide(PCR-PAGE-EB)staining and sequencing.Results:The frequency of MSI could reached 61.3% and that of LOH could reach 54.8%.The highest frequency of MSI was at locus of D9S168(32.3%);the highest frequency of LOH was at locus of D3S1289(21.4%).No correlation was found between MSI or LOH and the patients' age,sex,pathology type and metastastis,except that MSI was correlated with TNM stage of RCC(P
5.Application of "disharmony of stomach leads to insomnia" theory in acupuncture treatment for insomnia.
Wen-Li WANG ; Jian-Wu LIU ; Li-Guo CHEN ; Chen-Guang WANG ; Yan-Hong ZHANG
Chinese Acupuncture & Moxibustion 2014;34(12):1228-1230
When looking back to the literature regarding insomnia, it is believed that "disharmony of stomach leads to insomnia" is an important mechanism for the attack of insomnia. This theory is widely accepted in clinical practices by ancient and modern acupuncturists, thus the method treating insomnia from the stomach is developed. But deeper research on its mechanism is still lacking. Through correlation between stomach meridian and heart meridian, spleen and stomach being the pivot of rise and decline of qi movement and yin-yang and being the pivot of five zang-viscera housing spirit, modern abdomen-brain theory and experimental research, the mechanism of treating insomnia from the stomach is discussed in this paper, so as to be better to guide clinical acupoint-selection and treatment.
Acupuncture Therapy
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history
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China
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History, Ancient
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Humans
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Medicine in Literature
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Sleep Initiation and Maintenance Disorders
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physiopathology
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therapy
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Stomach
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physiopathology
6.Inhibitory effect of LZJ541, a novel small molecule inhibitor of STAT3, on the proliferation of hepatocellular carcinoma cells
Yi-chen LIU ; Ming JI ; Ting-ting DU ; Wen-qiang LIU ; Li LI ; Xiao-guang CHEN
Acta Pharmaceutica Sinica 2022;57(5):1396-1401
Signal transducer and activator of transcription 3 (STAT3) is an important regulatory factor of cell proliferation and metastasis, involved in the occurrence and development of a variety of malignant tumors, and it is one of the hot spots in the research of targeted anti-tumor drugs. Our group screened a novel benzobis (imidazole) structure small molecule compound LZJ541 through the screening model of Janus kinase (JAK)/STAT3 pathway inhibitors, which has definite STAT3 inhibitory activity. We examined the effect of LZJ541 on the proliferation of HepG2 and PC-3 cells by MTT assay
7.SSR information in Erigeron breviscapus transcriptome and polymorphism analysis.
Yin CHEN ; Cui-Ting LI ; Ni-Hao JIANG ; Sheng-Chao YANG ; Jun-Wen CHEN ; Jian-Wen YANG ; Guang-Hui ZHANG
China Journal of Chinese Materia Medica 2014;39(7):1220-1224
OBJECTIVEThe SSR information in the transcriptome of Erigeron breviscapus was analyzed in this study, in order to further develop new functional genes SSR markers laid a solid foundation.
METHODSSR loci were searched in all of 52,060 unigenes by using est_timmer. Perl program and SSR primers were designed by Primer3. Furthermore, 36 pairs of primers were randomly selected for the polymorphism analysis on 13 Erigeron breviscapus plants collected from different places.
RESULTA total of 3639 SSRs were found in the transcriptome of Erigeron breviscapus, distributed in 3260 unigenes with the distribution frequency of 6.99%. Di-nucleotide repeat was the main type, account for as much as 34.41% of all SSRs, followed by mono-nucleotide (31.41%) and tri-nucleotide repeat motif (28.08%). The di-nucleotide repeat motifs of AT/AT and AC/GT were the predominant repeat types (28.71%). The tri-nucleotide repeat motifs of AAT/AT was the predominant repeat types (7.94%). For validation the availability of those SSR primers, we randomly selected 36 pairs of primers for PCR amplification. Among them, 34 pair primers (94.44%) produced clear and reproductive bands, 19 pair primers showed polymorphism (52.78%), and 13 Erigeron breviscapus plants were divided into 2 groups.
CONCLUSIONThere are numerous SSRs in Erigeron breviscapus transcriptome with high frequency and various types, this will provide abundant candidate molecular markers for genetic diversity study and genetic map in this plant.
China ; DNA Primers ; genetics ; Erigeron ; classification ; genetics ; Genetic Variation ; Microsatellite Repeats ; Phylogeny ; Polymorphism, Genetic ; Transcriptome
8.Study on compatibility of Salviae Miltiorrhizae Radix et Rhizoma and Chuanxiong Rhizoma based on pharmacokinetics of effective components salvianolic acid B and ferulic acid in rat plasma.
Cui-ying ZHANG ; Hong ZHANG ; Yu DONG ; Wei-guang REN ; Heng-wen CHEN
China Journal of Chinese Materia Medica 2015;40(8):1589-1593
A study was made on the pharmacokinetic regularity of effective components salvianolic acid B and ferulic acid in Salviae Miltiorrhizae Radix et Rhizoma (SMRR) and Chuanxiong Rhizoma(CR) in rats, so as to discuss the compatibility mechanism of Salviae Miltiorrhizae Radix et Rhizoma and Chuanxiong Rhizoma. Rats were randomly divided into three groups and intravenously injected with 50 mg x kg(-1) salvianolic acid B for the single SMRR extracts group, 0.5 mg x kg(-1) ferulic acid for the single CR extracts group and 50 mg x kg(-1) salvianolic acid B + 0.5 mg x kg(-1) ferulic acid for the SMRR and CR combination group. The blood samples were collected at different time points and purified by liquid-liquid extraction with ethyl acetate. With chloramphenicol as internal standard (IS), UPLC was adopted to determine concentrations of salvianolic acid B and ferulic acid. The pharmacokinetic parameters of salvianolic acid B and ferulic acid were calculated with WinNonlin 6.2 software and analyzed by SPSS 19.0 statistical software. The UPLC analysis method was adopted to determine salvianolic acid B and ferulic acid in rat plasma, including linear equation, stability, repeatability, precision and recovery. The established sample processing and analysis methods were stable and reliable, with significant differences in major pharmacokinetic parameters, e.g., area under the curve (AUC), mean residence time (MRT) and terminal half-life (t(1/2)). According to the experimental results, the combined application of SMRR and CR can significantly impact the pharmacokinetic process of their effective components in rats and promote the wide distribution, shorten the action time and prolong the in vivo action time of salvianolic acid B and increase the blood drug concentration and accelerate the clearance of ferulic acid in vivo.
Animals
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Apiaceae
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chemistry
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Benzofurans
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blood
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pharmacokinetics
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Coumaric Acids
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blood
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pharmacology
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Drug Interactions
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Drugs, Chinese Herbal
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analysis
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pharmacokinetics
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Male
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Rats
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Rats, Sprague-Dawley
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Rhizome
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chemistry
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Salvia miltiorrhiza
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chemistry
9.Sequential transcatheter arterial chemoembolization-portal vein embolization in hepatectomy for liver carcinoma
Hao WANG ; Guang CHEN ; Haijun GAO ; Zhengjia YI ; Lianfang WEN ; Penghui WANG ; Yixin YANG ; Li ZHANG
Chinese Journal of Hepatobiliary Surgery 2017;23(6):412-413
This article presented our experience on transcatheter arterial chemoembolization (TACE) and portal vein embolization (PVE) before hepatic resection for huge hepatocellular carcinoma with cirrhosis.The preoperative future liver remnant/total estimated liver Volume (FLR/TELV) ratios of 5 patients were less than 40%,and preoperative TACE was implemented 3 weeks after PVE.In all these patients,right hepatectomy was successfully implemented.Preoperative TACE and PVE expanded the indication of hepatectomy,increased the safety of surgery and improved the curative rate.
10.Percutaneous intravascular stent treatment of hepatic venous outflow obstruction after pediatric liver transplantation
Haijun GAO ; Guang CHEN ; Hao WANG ; Penghui WANG ; Lianfang WEN ; Yixin YANG
Chinese Journal of Radiology 2014;48(10):853-857
Objective To evaluate the therapeutic effectiveness of percutaneous endovascular treatment of hepatic venous outflow obstruction (HVOO)after pediatric liver transplantation(LT).Methods From January 2008 to January 2013,10 children with obstruction of hepatic vein (HV) or inferior vena cava (IVC) anastomosis underwent percutaneous transluminal angioplasty (PTA) with balloon dilation or stent placement.The hepatic venous outflow obstruction occurred 10-455 days (median,125 days) after pediatric liver transplantation.According to the time of obstruction,the obstruction was divide into early onset (<1 month) and late onset(>1 month).The effectiveness of PTA was analyzed.Results Twenty-one procedures were performed.One treatment was ineffective,and technical and initial clinical success ratio was 95.2% (20/21) and 70.0% (7/10),respectively.In 3 cases with early onset after LT,operation was performed after unsuccessful PTA in 1 case.One patient who developed recurrent stenosis was treated with PTAS.The other patient died of acute rejection.Late onset after LT was found in 7 cases,who were treated with PTA or stent successfully.Conclusions In cases of venous outflow obstruction resulting from HV and/or IVC lesions after pediatric liver transplantation,percutaneous endovascular treatment with balloon dilation or stent placement is a safe and effective alternative treatment that results in midterm and long-term patency.Early-onset or hepatic veins combined with superior vena cava obstruction should be implanted with stents as early as possible.Late-onset or hepatic veins obstruction alone can be get better results with Balloon Dilatation.