Objective:To measure the serum levels of 25-hydroxyvitamin D in children with asthma, and to investigate the relationship between the serum levels of 25-hydroxyvitamin D and total immunoglobulin E (TIgE).Methods:From October 2013 to September 2014, 48 children with bronchial asthma were selected as asthma group, and 35 healthy children were selected as control group.Double-antibody radioimmunoassay(RIA) was used to detect the levels of serum 25-hydroxyvitamin D and TIgE.Results:The serum level of 25-hydroxyvitamin D in the asthma group was (35.86±14.31)nmol/L, which was significantly lower than that in the control group[(53.91±22.71)nmol/L], and IgE level in the asthma group was (331.66±223.67)IU/mL, which was significantly higher than that in the control group [(99.33±86.50)IU/mL], the differences were statistically significant( t=4.32, 2.36, all P<0.05). There was a negative correlation between serum 25-hydroxyvitamin D and IgE in the asthma group( r=-0.400, P<0.05). Conclusion:The serum levels of 25-hydroxyvitamin D may be associated with childhood asthma.The significant negative correlation between the levels of 25-hydroxyvitamin D and TIgE in children who had bronchial asthma indicates that asthma has a close relationship with allergy.Through increasing the concentration of 25-hydroxyvitamin D may prevent or treat child-hood asthma.