Objective To discuss the effect of early combined therapy of sertraline and psychotherapy on the rehabilitation of the patients with post-stroke depression (PSD)and observe its effect on plasma and platelet serotonin(5-HT). Methods All of 130 patients with PSD were divided into sertraline treatment group (65 cases) and routine treatment group (65 eases). Every patient was given routine treatment according to treatment guideline of cerebrovascular disease after admission. The sertraline treatment group received twelve-week sertraline treatment and psychotherapy, while the routine treatment group only received psychotherapy. Patients were evaluated by HAmilton rating scale for depression (HRSD) and serf-rating depression scale (SDS) before treatment and at the ends of the 4th and 8th week treatment, respectively. Meanwhile, the levels of plasma and playlet 5-HT of every patient were measured by the enzyme immunoassay methods. Results The scores of HRSD and SDS were lower in sertraline treatment group than those in routine tretment group at the ends of the 4th and 8th week. The rate of decrease of HRSD and SDS in sertraline treatment group was higher than that in routine treatment group at the end of the 8th week (Pall<0.01).The levels of platelet 5-HT in sertraline treatment group was lower at the ends of the 4th and 8th week than those before, treatment (P< 0.01).No significant change in plasma 5- HT level could be observed before and after treatment in two groups(P> 0.05). Conclusions Early treatment by sertraline can release the symptom of PSD and promote rehabilitation of PSD patients. The sertraline treatment can reduce the level of platelet 5- HT in PSD patients. However, the effect of plasma 5-HT value is not clear.

Objective To summarize the clinical features of angina pectoris after thoracotomy. Methods Eight males with a mean age of 60? 5. 4 years were reported and analyzed, of which four patients underwent esphagectomy, three patients esophagogastrectomy and 1 patient pneumonectomy. Results Three cases were attacked angina pectoris after surgery 2~4 h, three cases in 5~12 h, one case in 36 h, and one case in 72h. Acute mocardial infarction of different degrees happened in five cases, and one case died. Conclusion Angina pectorls after thoracotomy is very dangerous to the patlents and not easily distinguished from chest pain after thoracotomy. It's important for the surgeons to analysis comprensively , diagnose as early as posslble and treat immediately.

Objective To study the effects of Toll-like receptor 4(TLR4) on oxidized low density lipoprotein ( ox-LDL) induced macrophage apoptosis and its possible mechanism .Methods THP-1 derived macrophages were divided into four groups including untreated control group , ox-LDL treated group , ox-LDL+LPS treated group and tunicamycin treated group .MTT assay and flow cytometry analysis were performed to measure cell vitality and cell apoptosis , respectively .Oil red O staining was used to observe the phagocytosis of lipids by macrophages .The persistent and intense endoplasmic reticulum ( ER) stress markers were de-tected by analyzing the expression of glucose-regulated protein 78 ( GRP78 ) and CCAAT/enhancer-binding protein homologous protein ( CHOP) at mRNA and protein levels by q-RT-PCR and Western blot .Small in-terfering RNA ( siRNA) was used to silence the expression of TLR 4 to further elucidate its possible mecha-nism.Results Flow cytomotry and MTT assay showed that the number of apoptotic cells in ox-LDL+LPS treated group were increased more significantly than that in ox-LDL treated group (P<0.01), and cell apop-tosis in both two groups were greater than that in control group (P<0.01).Compared with control group, the expression of GRP78 and CHOP at mRNA and protein levels were up-regulated in ox-LDL+LPS treated group and ox-LDL treated group (P<0.01), and the expression of GRP78 and CHOP in ox-LDL+LPS treated group was significantly higher than that in ox-LDL treated group (P<0.01).Silenced expression of TLR4 al-leviated the endoplasmic reticulum stress (P<0.05).Conclusion Increased expression of CHOP contribu-ted to cell apoptosis .TLR4 might promote ox-LDL induced macrophage apoptosis through accelerating endo-plasmic reticulum stress .

Objective To observe the change of comprehensive agitating coefficient in the intracerebral hem -orrhage( ICH) patients monitoring by non-invasive cerebral edema and dynamical method ,and to find out dynamical change of cerebral edema in patients with ICH ,and to evaluate the specificity and sensitivity of non-invasive dynami-cal brain edema monitor in ICH treatment .Methods The change of comprehensive agitating coefficient in 130 patients was detected by non-invasive dynamical brain edema monitor at day 1,3,5 and 7 from ICH onset.Results The comprehensive agitating coefficient in focus side was lower than the unaffected side in both groups at the first day .The coefficient in the focus side was higher than the unaffected side at the third day ,and then became approximately equal at day 5 and basically normal at day 7.The comprehensive agitating coefficients in focus side were significantly differ-ent to unaffected side at day 3,5(P<0.01),and quite different at day 1,7(P<0.05).Conclusion The compre-hensive disturbance coefficient takes on dynamical changes in the ICH and reflects the course of edema formation . Cerebral edema monitoring by non-invasive and dynamical method can evaluate the course of cerebral edema ,and has a guiding role to the therapy of the ICH .

Objective To observe the hypoglycemic mechanism of total saponins of Momordica charantia (MC) in type 2 diabetes mellitus (DM) rats. Methods Among the selected 60 male Specific Pathogen Free (SPF)rats, 8 were random- ly chosen as control group, while others were fed with high fat and high glucose diet following streptozotocin injection from caudal vein 8 weeks after to construct type 2 DM models. After the DM models were successfully built, rats were then ran- domized into five groups: DM control group (n=8), the metformin group (n=8) and three groups of total saponins of MC with different dosage (n=8 in each group). The total saponins of MC groups include DM rats administrated with total saponins of MC 100, 200, 400 mg/(kg·d) for 8 weeks, the metformin group include DM rats administrated with metformin 50 mg/(kg·d) for 8 weeks. At the end of the experiment, the fasting blood glucose and insulin were examed. At the same time, a part of pan- creas islet, liver and skeletal muscle were preserved. The pancreas islet structure, the hepatic glycogen and Glucose trans- porter 4 (GLUT4) expression were observed by electron microscope, glycogen dyeing and immunoblot respectively. Results After 8 weeks treatment, compared to type 2 DM control group, fasting blood glucose and insulin values in MC groups were reduced more obviously. However, skeletal muscle GLUT4 expression level, insulin granules and hepatic glycogen increased obviously in MC groups. Conclusion Total saponins of MC has hypoglycemic effect. It’s mechanisms maybe include pro- moting the hepatic glycogen synthesis, inhibiting the hepatic glycogen decomposition and promoting insulin sensitivity by in- creasing peripheral tissue’s GLUT4 expression.

Aim To investigate the changes of content of L-carnitine (LC ), acetyl-L-carnitine (ALC ) and propionyl-L-carnitine (PLC )in patients with diabetes and its complications.Methods By replicating meth-od of pre-column derivatization HPLC,the contents of plasma LC,ALC,PLC were detercted in normal sub-jects and in patients of diabetes mellitus,diabetic reti-nopathy, diabetic nephropathy, diabetic peripheral neuropathy,diabetic hypertension,diabetic coronary heart disease.Results The concentration of LC in normal subjects and in patients of diabetes mellitus, diabetic retinopathy, diabetic nephropathy, diabetic peripheral neuropathy,diabetic hypertension,diabetic coronary heart disease were (41.01 ±8.05 )μmol · L-1 ,(36.72 ±8.23 )μmol · L-1 ,(33.3 1 ±6.26 )μmol·L-1 ,(33.81 ±5.61 )μmol·L-1 ,(33.57 ± 6.67 )μmol · L-1 , (33.67 ±5.36 )μmol · L-1 , (32.87 ±6.05 )μmol · L-1 respectively.The plasma LC concentration in diabetic group was significantly lower than that of normal control group (P<0.05 ). Moreover,the LC concentration in other groups of dia-betic complications was lower than that in diabetic group, and LC concentration showed no significant difference in various groups of diabetic complications. There was no significant difference in the plasma con-centrations of ALC and PLC of diabetes group and the diabetic complication groups too.Conclusion In Chi-nese normal subjects,patients with diabetes and dia-betic complications,the plasma concentrations of LC in diabetic patients are lower than normal,and plasma concentration of LC in diabetic complications are lower than that in patients with diabetes mellitus.

Objective To study the expression of CD163 in macrophages and sCD163 level in the serum of neonatal rat model of Escherichia coli (E. coli) sepsis. Methods A total of 72 specific-pathogen-free (SPF) neonatal rats (P7) were randomly and equally assigned into experiment group and control group. E. coli was injected peritoneally and the sepsis model was established in the experiential group while normal saline (NS) was injected in the control group. Samples were collected at 2, 4, 6, 12, 24 h and 48 h after the treatment. CD163 expression in macrophages of lung and liver tissues were tested using immunohistochemical(IHC) method, and the dynamic changes of sCD163 concentration in the serum were monitored usingenzyme-linked immunosorbent assay (ELISA) method. Results In the experiment group, CD163 expression in macrophages of lung and liver were gradually decreased at eachtime point (P <0. 001). At 2 h, CD163 expression in macrophages showed no significant differences between the two groups (P >0. 05). At 4 h and later timepoints, the differences were statistically significant (P <0. 001) . Meanwhile, sCD163 in the serum increased gradually (P <0. 01). At 2 h, sCD163 in the serum showed no significant differences between the two groups (P >0. 05). At 4 h and later timepoints, the differences were statistically significant (P <0. 001). Conclusions CD163 plays an important role in sepsis.

Objective To determine the Golgi dispersal in radiation damaged cells and the protective effect of vanillin derivatives.Methods Immunofluorescence, cell cycle analysis of flow-cytometry,Western blot,and clone formation were used.Results Immunofluorescence observation showed that the Golgi dispersal caused by 2 Gy 60 Coγ-ray was significantly increased in a dose-dependent manner in the range of 4-10 Gy as was demonstrated by the fact that the Golgi area was significantly increased. When the irradiated cells were treated with the radioprotective agent VND3207, a vanillin derivative,the Golgi dispersal induced by radiation was significantly reduced.The radiation-induced Golgi dispersal was also displayed in a pattern of time-course after irradiation in the HeLa cells, and persisted at least to 36 h post-irradiation. Cell cycle test results indicated that the Golgi dispersal was not associated with the G2/M arrest triggered by radiation-induced DNA damage response.VND3207 could promote cell survival by plate colony formation assay.Conclusion The Golgi dispersal can be caused byγ-ray irradiation in a dose-and time-dependent manner, and VND3207 can provide a good protection against radiation injury associated with inhibited Golgi dispersal.

Objective To evaluate the occurrence and prevention of pinch-off syndrome in post-operative complications of totally implantable central venous port.Methods From October 2003 to September 2016,628 cases underwent implantable central venous port via the subclavian vein using the landmark venipuncture technique.From February 2014 to September 2016,106 cases underwent implantable central venous port gaining subclavian venous access.Retrospective analysis of two groups of pinch-off syndrome after totally implantable central venous port.Results pinch-off syndromne were not found in the uhrasound-guided group,pinch-off syndrome occurred in 9 patients of 628 cases underwent implantable central venous port via the subclavian vein using the landmark venipuncture technique during follow-up period.Conclusions Subclavian venous access cannulation on the lateral side of mid-clavicular line by ultrasound guidance was found to be associated with absence of pinch-off syndrome.This method appears to reduce or prevent pinch-off syndrome occurrence after implantable central venous port,therefore improving the safety of the long-term management of implantable central venous port.

The present article deals with the result of early repair of 105 cases of cleft palate within the first two years of life.The result is excellent without fistulae,ruptures or deaths.The early repair of the cleft palate has the advantages of small blood loss during operation,little postoperative reaction and better healing.The key points of surgical procedure and postoperative management are introduced in details.The safety of the early operation and its influence on the development of maxillofacial growth are also discussed.The deficiency of maxillofacial growth is related to surgical procedures of cleft lip and palate besides the congenital factors.Early repair of cleft palate enables the child to develop normal speech and is advantageous to psychological development of the child.