1.Treatment of post-menarche idiopathic central precocious puberty in girls with combined gonadotropin-releasing hormone analog and growth hormone.
Yun LI ; Li LIANG ; Li-ying SUN ; Guan-ping DONG
Chinese Journal of Pediatrics 2005;43(8):627-628
Age Determination by Skeleton
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Body Height
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drug effects
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Child
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Child Development
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drug effects
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Drug Therapy, Combination
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methods
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Female
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Gonadotropin-Releasing Hormone
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administration & dosage
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analogs & derivatives
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therapeutic use
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Growth Hormone
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administration & dosage
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therapeutic use
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Humans
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Menarche
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Puberty, Precocious
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diagnosis
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drug therapy
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physiopathology
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Treatment Outcome
2.A case of beta-ketothiolase deficiency.
Jian-ying ZHAN ; Li LIANG ; Guan-ping DONG
Chinese Journal of Pediatrics 2006;44(9):703-704
5.A case report of Naja atra bitten poisoning in northern China.
Ping HAN ; Si-zhuo PANG ; Xiang-dong GUAN ; Jie-ru WANG
Chinese Journal of Industrial Hygiene and Occupational Diseases 2013;31(9):706-706
Animals
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China
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Elapidae
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Humans
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Male
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Snake Bites
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Snake Venoms
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poisoning
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Young Adult
6.Experimental Colitis Model Induced by DSS and TNBS in Rats:A Comparative Study
Ping ZHAO ; Lei DONG ; Jinyan LUO ; Haitao GUAN ; Yahua SONG ; Jun GONG
Chinese Journal of Gastroenterology 2015;(11):667-671
Background:As the empirical studies on human body are restricted extremely,the establishment and selection of suitable animal models are important for researches on ulcerative colitis( UC ). Aims:To compare the symptoms and colonic pathology of rat models with experimental colitis induced by dextran sulfate sodium( DSS ) and trinitrobenzene sulfonic acid( TNBS),so as to provide a reference for selecting animal models in UC-related studies. Methods:Drinking 4% DSS freely for 7 days or intrarectal administration of single dose 100 mg/kg TNBS-50% ethanol were used to establish experimental colitis model in Sprague-Dawley rats. The disease activity index( DAI)was assessed dynamically during the course of experiment. The whole colon was removed in batches for measurements of colonic damage score and activity of myeloperoxidase(MPO)at different time points. Results:The DAI score reached the peak at the 7th day and the 2nd day in DSS group and TNBS group,respectively,and decreased gradually afterwards. Six and one deaths occurred during the experimental course in DSS and TNBS groups,respectively. In DSS group,the duration of inflammation was short,the colonic injury was moderate and recovered after drug withdrawal. At the 18th day,the colonic damage score and MPO activity was 0. 25 ± 0. 50 and(0. 80 ± 0. 33)U/g,respectively,and no significant differences were seen between DSS group and normal control group. In TNBS group,the duration of inflammation was longer and the colonic injury was more severe. At the 21st day,the colonic damage score and MPO activity was 3. 60 ± 0. 55 and( 1. 60 ± 0. 39 ) U/g, respectively,and chronic inflammation was observed histologically. Conclusions:Both DSS and TNBS can induce experimental colitis model in rats. The course of TNBS-induced colitis model presents a transformation of acute to chronic inflammation,and may be more suitable for treatment-related studies of UC.
7.Recent advances in drug screening methods of SARS-CoV-2 spike protein
Li-de HU ; Chuan-feng LIU ; Ping LI ; Guan-yu DONG ; Xin-yong LIU ; Peng ZHAN
Acta Pharmaceutica Sinica 2024;59(2):298-312
The pandemic of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a serious impact on global public health and the economy. SARS-CoV-2 infiltrates host cells
8.Antiviral Effect of Interferon-Induced Guanylate Binding Protein-1 against Coxsackie Virus and Hepatitis B Virus B3 in Vitro
Yin-ping, LU ; Bao-ju, WANG ; Ji-hua, DONG ; Zhao, LIU ; Shi-he, GUAN ; Meng-ji, LU ; Dong-liang, YANG
Virologica Sinica 2007;22(3):193-198
Guanylate binding protein-1(GBP-1) is an interferon-induced protein. To observe its antiviral effect against Hepatitis B virus (HBV) and Coxsackie virus B3 (CVB3), we constructed an eukaryotic expression vector of human GBP-1(hGBP-1). Full-length encoding sequence of hGBP-1 was amplified by long chain RT-PCR and inserted into a pCR2.1 vector, then subcloned into a pCDNA3.1(-) vector. Recombinant hGBP-1 plasmids and pHBV1.3 carrying 1.3-fold genome of HBV were contransfected into HepG2 cells, and inhibition effect of hGBP-1 against HBV replication was observed. Hela cells transfected with recombinant hGBP-1 plasmids were challenged with CVB3, and viral yield in cultures were detected. The results indicated that recombinant eukaryotic expression plasmid of hGBP-1 was constructed successfully and the hGBP-1 gene carried in this plasmid could be efficiently expressed in HepG2 cells and Hela cells. hGBP-1 inhibit CVB3 but not HBV replication in vitro. These results demonstrate that hGBP-1 mediates an antiviral effect against CVB3 but not HBV and perhaps plays an important role in the interferon-mediated antiviral response against CVB3.
9.Construction and Characterization of a Hepatitis B Virus Replicon
Yin-ping, LU ; Bao-ju, WANG ; Ji-hua, DONG ; Zhao, LIU ; Shi-he, GUAN ; Meng-ji, LU ; Dong-liang, YANG
Virologica Sinica 2007;22(1):8-13
To establish a replication cellular model of hepatitis B virus (HBV) and determine its application in antiviral drug evaluation,we constructed an expression plasmid which contained 1.3 copies of the HBV genome,and measured the level of viral replication after transient transfection in Huh7 cells.We then observed the effect of antiviral drug administration.1.3 fold of the HBV(ayw) gene fragment was cloned into pCR2.1 by PCR and restriction endonuclease digestion.The recombinant plasmid was trans ient transfected into Huh7 cells,HBsAg,HBeAg and HBV DNA in supernatant of Huh7 cells were measured by ELISA and real-time PCR respectively; intracellular HBV replicative intermediates and intracellular HBV transcripts were detected by Southern blot and Northern blot respectively.The antiviral effect of adefovir,a novel anti-HBV nucleotide analogue,was evaluated in this cellular model system.The results indicated that a recombinant plasmid of HBV replicon was constructed successfully; the HBV genome carried in plasmid pHBV1.3 could efficiently replicate and be expressed in Huh 7 cells,adefovir could inhibit HBV replication in this cellular model,and the inhibition was dosage-dependent.The conclusion is HBV replicon,which can initiate viral replication efficiently in hepatoma cells,may be a useful tool in the study of HBV replication and antiviral drug.
10.Distribution of rag genotypes of Porphyromonas gingivalis in patients with chronic periodontitis.
Guan-hua WANG ; Dong-mei ZHANG ; Ya-ping PAN
West China Journal of Stomatology 2009;27(2):168-171
OBJECTIVETo investigate the distribution of rag genotypes of Porphyromonas gingivalis (P. gingivalis) in chronic periodontitis patients.
METHODSSubgingival plaque samples were collected from 50 chronic periodontitis patients. The occurrence of P. gingivalis was determined by polymerase chain reaction (PCR) using 16S rDNA-specific primers. Distribution of rag genotypes was assessed in P. gingivalis positive samples by PCR.
RESULTSThe occurrence of P. gingivalis was 70.7%, and the distribution of four rag genotypes among P. gingivalis positive samples was as follows: rag-1 60.4%; rag-2 23.6%; rag-3 44.3%; rag-4 15.1%, respectively.
CONCLUSIONP.gingivalis with various rag genotypes was present in subgingival plaque samples from chronic periodontitis patients, and P. gingivalis with rag-1 and rag-3 were more predominant in chronic periodontitis patients, which may be associated with the development of periodontitis.
Adult ; Chronic Periodontitis ; DNA-Binding Proteins ; Dental Plaque ; Female ; Genotype ; Humans ; Male ; Periodontitis ; Polymerase Chain Reaction ; Porphyromonas gingivalis