0.05),but age,Singh's index and Garden's reduction index had significant relation to failure of DHS(P

Microfluidic chip exhibit a great promising development in clinical diagnosis and disease screening due to their advantages of precise controlling of fluid flow,requirement of mini amount sample,rapid reaction speed and convenient integration.A lot of demonstrations on the diagnostic applications related to genes,proteins,and cells have been reported because of their advantages associated with miniaturization and automation.Here,the applications and developments of on-chip nucleic acid amplification and analysis,protein analysis and detection,cell selection and cell drug screening were discussed.Microfluidic chip can provide an easy integration platform for biomarkers in a high throughput and accurate detection.

GDF-15,a distant member of the TGF-superfamily,is identified as an apoptotic accelerat- ing,anti-tumorigenesis and nerve2nutritional factor in varied injures and tumors and has cardioprotective ac- tivity.The characteristics and roles of GDF-15 gene/protein and antibodies are expounded besides the rela- tionship between GDF-15 serum level/genetypes and CRC.It is also discussed here that some antitumorigenic substances inducing GDF-15 in CRC tissues and CRC cells.

Aim To investigate change in Cl- channels of hypertensive rats aortic smooth muscle cells. Methods 2 kidney-2 clip renovascular hypertensive rats(RVHR) model was established. In thoracic aorta smooth muscles from the hypertensive rats 1～12 weeks after operation,the changes of tension of aortic rings were recorded in vitro. Effects of Cl- channel blookers,DIDS(4,4-diisothiocyanato-stilbene-2,2'disulphonate)and NPPB[5-nitro-2-(3-phenylpropy-lamino) benzonic acid], in different concentration on contractile response of hypertensive rats aorta smooth muscle induced by 10 ?mol?L -1 phenylephrine were observed.Results The difference of inhibitory effects of 300 ?mol?L -1 DIDS and 100 ?mol?L -1 NPPB on Phe-induced contractile response between RVHR and sham-operated rats was not evident.Inhibitory effects of 300 ?mol?L -1 DIDS and 100 ?mol?L -1 NPPB on contractile response of hypertensive rats aorta smooth muscle induced by 10 ?mol?L -1 phenylephrine were lower than those of sham-operated rats in 8 weeks and 12 weeks after operation. With extension of time after operation and gradual increase of blood pressure in RVHR,inhibitory effects of DIDS and NPPB on Phe-induced contractile response gradually reduced.Conclusion Action of DIDS-sensitive and NPPB-sensitive Cl- channels in hypertensive rats aortic vascular smooth muscle cells changes. DIDS-sensitive and NPPB-sensitive Cl- channels play an important role in development and maintenance of hypertension.

To investigate constituent ratio and drug resistant status of pathogenic bacterium in SCI patients with urinary infection.The germ cultures of midstream urine samples and identifications to 456 stains pathogenic bacterium were conducted,susceptibility to antibiotics were tested.The results showed that G- baccili occupied absolute predominance among pathogenic bacterium.It is very important to select antibiotics rational for urinary infections of SCI patients according to the results of susceptibility tests.

ObjectiveTo establish a method to induce heterotopic ossification (HO) after spinal cord injury. Methods10 New Zealand White rabbits underwent a complete transection of the thoracic (T10) spinal cord. Just after transection, the right hind limb of every rabbit was immobilized with a plaster support to keep the knee in extension and the hip unrestricted. The plaster was temporarily removed once a day (six times per week) to allow the knees passively mobilized at the maximum range for 5 min. Local swelling, local skin temperature and grade of ossification of the paraplegic limbs were observed. After 5 weeks, the rabbits were sacrificed, and the quadriceps muscles of the right hind limbs were removed for the histological examination. ResultsAfter 5-week immobilization and temporary passive mobilization, HO was successfully induced in all 10 rabbits. There was local skin temperature increase and local swelling in the right hind limbs. The histological changes were similar to those observed in clinical HO. Conclusion5-week immobilization and temporary passive mobilization approach may successfully induce HO, and it may be used to study the pathogenesis of HO after spinal cord injury.

Objective To evaluate the parametric features of image textures on 18F-FDG PET/CT for the differentiation between malignant and benign pulmonary nodules and compare the diagnostic performance of these parameters with SUVmax.Methods 18F-FDG PET/CT images of 170 patients (102 males,68 females,age range:29-81 (mean 59)years) with pulmonary nodules were retrospectively evaluated.Eightynine pulmonary nodules (230 slices) were malignant and 81 (193 slices) were benign.The pulmonary nodules were contoured on CT images and mapped to the co-registered PET images.Thirteen parameters of textural features were extracted and SUVmax was measured.Logistic regression analysis was used to identify the significant texture parameters and create a regression model.The efficacy of the textural features and SUVmax to distinguish between malignant and benign pulmonary nodules was evaluated by ROC curve analysis.The textural features of squamous cell carcinoma and adenocarcinoma were compared via the Mann-Whitney u test.The sensitivity and specificity of the textural features and SUVmax for the differential diagnosis were compared with x2 test.Results Logistic regression model identified 4 textural features (skewness (β =1.7058),kurtosis (β =-1.0989),angular second moment (ASM,3 =-4.4140) and strength (β =0.5626) ; all P ＜ 0.05) to have significant correlation with the malignancy of lung nodules.The AUC of ROC curve was 0.775 (95％ CI0.732-0.819; P＜0.001) with the sensitivity of 89.6％ (206/230) and specificity of 50.8％ (98/193).ASM and strength had statistically significant differences between squamous cell carcinoma and adenocarcinoma [ASM:0.0303 (95％ CI 0.0392-0.0724) vs 0.0594 (95％ CI 0.0721-0.0947) ; strength:2.4714 (95％ CI 2.4632-4.1050) vs 1.5945 (95％ CI 1.9003-2.4652) ; u =3082.0 and 3115.0,both P＜0.01].The AUC of SUVmax-based diagnosis was 0.757 (95％ CI 0.711-0.802 ; P ＜ 0.001) with the sensitivity of 80.9 ％ (186/230) and specificity of 50.3 ％ (97/193) at a cut-off value of 2.5.The sensitivity of the textural features was superior to SUVmax in differentiating malignant from benign pulmonary nodules (x2 =6.903,P ＜ 0.01).Conclusions Image textural parametric features extracted from 18 F-FDG PET/CT are more sensitive to differentiate between malignant and benign pulmonary nodules compared to SUVmax.They might also be useful to discriminate between different pathological types of lung cancers.

Objective To investigate the therapeutic effect of nasal endoscope surgery on chronic sinusitis and nasal polyps in geriatric patients.Methods A total of 132 geriatric patients with chronic sinusitis and nasalpolyps underwent nasal endoscope surgery in our hospital from Jun.2010 to Jun.2014,and the therapeutic effect was investigated.Results In the 132 cases,92 cases were cured (69.7％).Therapeutic effects were excellent in 33 cases (25.0％),and invalid in 7 cases (5.3％).The total effective rate was 94.7％.The total effective rate was 100.0％ in type Ⅰ,97.4％ in type Ⅱ and 84.8％ in type Ⅲ.The total effective rate was higher in type Ⅰ and Ⅱ than in type Ⅲ (x2=3.506 and 6.218,P=0.035 and 0.012).Local complications occurred in 10 cases (7.6％),including 1 case with nasolacrimal duct injury,2 cases with orbital hematoma,5 cases with nasal adhesion,1 case with surgical cavity hemorrhage and 1 case with sinus atresia.Conclusions The therapeutic effects of nasal endoscopesurgery on chronic sinusitis and nasalpolyps are good in geriatric patients,especially in treating chronic sinusitis and nasalpolyps of type Ⅰ and Ⅱ,and the postoperative complications are less,which is worth clinical application.

Wound healing is a complex and highly regulated process to maintaining the skin barrier function. Wounds of diabetic patients are hard or even not healing. Non-healing diabetic foot ulcers can lead to lower-extremity amputations. Diabetic wound healing problem is the main complication that leads to high disability rate of diabetes and can threaten the lives in severe cases. The healing of skin wounds requires the synergy of multiple factors to restore the injured skin to its barrier function. The mechanisms that cause it difficult to heal diabetic wounds are complex, including oxidative stress, chronic inflammation, decreased neovascularization, peripheral neuropathy, and imbalance of extracellular matrix accumulation and remodeling. This review classifies mechanisms of diabetic wound healing and provides a reference for its further research.

Mitochondria play a key role in cell metabolism. In addition to synthesizing ATP, they also participate in many physiological and pathological processes, including apoptosis, inflammation, oxidative stress, neuronal disease, tumor development, and aging. Most gene transcription of mitochondrial proteins occurs in the nucleus, so the biogenesis of mitochondria and the maintenance of mitochondrial homeostasis mainly depend on the expression of nuclear genes (nDNA) and mitochondria-nucleus interactions. Conversely, mitochondria can affect the expression of nuclear genes through nuclear transcription factors, a process called mitochondrial retrograde signaling. This review summarizes the research progress on mitochondria-nucleus retrograde signaling and its regulation, including the ways by which mitochondria regulate nuclear genes and affect biological processes, and discusses new strategies for the treatment of diseases that involve mitochondrial retrograde signaling in disease pathology.