OBJECTIVE:To provide reference for the evolvement of the current drug marketing license system in China. METHODS: The registry classification, the drug registration procedure and the combination of marketing license and production license both at home and abroad were analyzed comparatively. In addition, the necessity and feasibility of the drug marketing license system in China were analyzed. RESULTS & CONCLUSIONS: The current drug marketing license system that centered on license No. goes against the investigation of legal ability, drug administration mode, medicine technology transfer system and original equipment manufacture thus remains be reformed. There is still difficulty for the enforcement of drug marketing licensor system, yet it should be carried out step by step until its establishment.

Rheumatoid arthritis ( RA) was a common autoimmune disease characterized with main clinical manifestations of chronic inflammation in joints.Anti-carbamylated proteins(anti-CarP)antibody, as a promising bio-marker, played a crucial role in the diagnosis of RA patients and the screening of pre-RA patients .In this article , recent progress on the study of anti-CarP antibody and RA related diseases has been reviewed , which was expected to provide new references for the diagnosis and treatment of related clinical diseases .

The high-intensity interval training often consisted of groups of interval high-intensity exercise of exhaustion or above 90%maximal oxygen consumption, which can be applied to improve aerobic capacity. Adaption of skeletal muscles might be resulted from high-intensity interval training, including recruitment, glucose metabolism and activity of mitochondrial oxidase, etc., and improve maxi-mum oxygen uptake. It has been used for physical fitness, such as weight reduction, rehabilitation of heart diseases and diabetes mellitus, etc.

Objective To investigate the clinical efficacy of mind- and qi-regulating acupuncture in treating postoperative gastroplegia.Methods Thirty-six patients with postoperative gastroplegia were randomly allocated to treatment and control groups, 18 cases each. The control group received conventional acupuncture and the treatment group, acupuncture at points for regulating mind and qi (Baihui, Sishencong and Yindang) in addition. Pre- and post-treatment Hamilton Depression Rating Scale (HAMD) scores and gastric drainage volumes, and the number of treatments and the recovery rate were recorded in the patients. Comparisons of them were made between the two groups.Results There was a statistically significant pre-/post-treatment difference in the HAMD score in the two groups (P<0.01). There was a statistically significant post-treatment difference in the HAMD score between the treatment and control groups (P<0.05). There was a statistically significant pre-/post-treatment difference in gastric drainage volume in the two groups (P<0.01). There were statistically significant differences in the number of treatments and the recovery and marked efficacy rate between the treatment and control groups (P<0.05).Conclusions Mind- and qi-regulating acupuncture helps recovery and can also reduce the number of treatments in gastroplegia patients.

OBJECTIVE To investigate the clinical characteristics and the present condition of infective diabetic foot. METHODS A total of 100 cases with infective diabetic foot were classified into the wet gangrene and composite gangrene, and treated by the systemic and local modality in combination. RESULTS The cure rate of the wet gangrene and composite gangrene was 29.5% and 18.2%, respectively and the effective rate was 83.3% and 68.2%, respectively. CONCLUSIONS The combined therapy is effective for the treatment of diabetic foot.

Objective To investigate the clinical characteristics and treatment of senile patients with lumbar disc protrusion . Methods The clinical data of 56 cases of senile lumbar disc protrusion from 1990 to 2000 were reviewed retrospectively. Results 48 patients had been followed for 6 months to 7.5 years with average 4 years. Excellent and good results were 41 cases (85.4%).Conclusions There was atypical symptom in the senile patients with lumbar disc protrusion. CT was an important methods for diagnosis. The after concomitant diseases were controlled, resecting half vertebral lamina and enlarging fenestration were performed. The therapeutic results were satisfactory.

Drugs, Chinese Herbal ; therapeutic use ; Histiocytic Necrotizing Lymphadenitis ; drug therapy ; Humans

Objective:To investigate the effect of miRNA-1-3p (miR-1-3p) on expression of myocyte enhancer factor 2A (MEF2A) and the biological function of osteosarcoma cells.Methods:The tumor tissues and adjacent normal tissues of 20 patients with osteosarcoma who were clinically diagnosed in Shanxi Provincial Cancer Hospital from January 2019 to January 2020 were collected, and the expression of miR-1-3p in the samples was detected by real-time fluorescent quantitative polymerase chain reaction (qRT-PCR). The expression of miR-1-3p in osteosarcoma cell lines U2-OS, SAOS-2, MG63, SW1353 and human normal osteoblast cell line hFOB1.19 was detected by qRT-PCR, then the cell line with the lowest expression of miR-1-3p was selected for follow-up experiments. An overexpression miR-1-3p vector was constructed (miR-1-3p mimcs). The miR-1-3p overexpression group was transfected with miR-1-3p mimcs, and the control group was transfected with empty vector (miR-1-3p nc). CCK-8 method was used to detect the proliferation activity of cells; flow cytometry was used to detect the changes of cell apoptosis and cell cycle. miRwalk database was used to predict the miR-1-3p target gene, and the target gene was verified by dual-luciferase reporter gene assay; Western blot was used to detect the expression of MEF2A protein in cells of each group.Results:Compared with adjacent tissues, the expression of miR-1-3p in osteosarcoma tissues was down-regulated (0.31±0.14 vs. 0.62±0.21), and the difference was statistically significant ( t = 5.31, P<0.01). The expression of miR-1-3p in U2-OS cells was the lowest; compared with the control group, the proliferation activity of U2-OS cells was inhibited in miR-1-3p overexpression group (48 h absorbance value 0.56±0.01 vs. 0.77±0.03, t = 2.77, P<0.01; 72 h absorbance value 0.87±0.02 vs. 1.40±0.03, t = 2.93, P<0.01); G 1/S cell cycle arrest increased [G 1 phase (38.24±0.55)% vs. (32.11±0.80)%, t = 9.27, P = 0.01; S phase (61.24±0.90)% vs. (67.78±0.83)%, t = 7.52, P = 0.02]; early apoptotic rate increased [(11.20±0.12)% vs. (1.50±0.12)%, t = 2.91, P<0.05], miRwalk database predicted that the miR-1-3p target gene was MEF2A. The result of dual-luciferase reporter gene assay showed that miR-1-3p bound to MEF2A 3'UTR, and the luciferase activity of U2-OS cells in miR-1-3p overexpression group was lower than that in the control group (renilla luciferase/firefly luciferase activity ratio 0.53±0.06 vs. 1.00±0.04, t = 4.04, P < 0.05). Western blot showed that the expression of MEF2A protein in U2-OS cells of miR-1-3p overexpression group was lower than that of the control group (protein relative expression 0.41±0.14 vs. 0.77±0.12, t = 3.93, P < 0.05). Conclusions:The low expression of miR-1-3p may be associated with the proliferation, apoptosis and cycle changes of osteosarcoma cells. miR-1-3p can negatively regulate the expression of MEF2A protein and regulate the occurrence and development of osteosarcoma.

Objective To investigate the effect of life style combined with dietary intervention on non-alcoholic fatty liver disease(NAFLD).Method One hundred and twenty five subjects of NAFLD with overweight and obesity,aged 30-60 years,were enrolled in the study from the group for physical examination in Health Administration Center of our hospital during 2006-2007.They were diagnosed to be 48 mild,70 moderate and 7 severe cases by ultrasonic tomography.A regime of dietary intervention combined with exercise was planned according to 3 d dietary survey before intervention.They were asked to follow the therapeutic menus and exercise instruction during 12 mon of the study.We followed up the subjects and gave them instructions regularly in the whole period of study.Results After 12 mon interven-tion,the body weight(BW),body mass index(BMI),waist-hip ratio(WHR),serum total cholesterol(TC),triglycerides(TG),low-density lipoprotein cholesterol(LDL-C) and alanine glutamate aminotransferase(ALT) of the subjects were decreased significantly.High density lipoprotein cholesterol(HDL-C) was not changed.By ultrasonic tomography,16% of the subjects recovered to normal,and 77 moderate to severe cases deceased to 31,with reduction rate 59.7%.Conclusion Good therapeutic effects were observed after 12 mon of dietary intervention combined with rational exercise.

Sarcopenia is a series of syndromes featured by the steady and involuntary loss of skeletal muscle mass and function with aging.It can lower the quality of life and increase the risk of disability and mortality.Deficiency in some nutrients such as amino acids and proteins and accordingly the decreased in the synthesis of muscle protein and changes in muscle tissues may contribute to the development of sarcopenia.Therefore,nutritional interventions may play a role in the prevention and treatment of sarcopenia.