1.The clinical study of orthotopic liver transplantation patients with severe sepsis
Shunwei HUANG ; Xiangdong GUAN
Journal of Chinese Physician 2009;11(9):1194-1196
Objective To improve the treat effect of the orthotopic liver transplantation patients with severe sepsis. Methods Fif-ty-six post-surgery patients were enrolled in this study. Patients were divided into two groups. One was non-OLT group (A group) and the other was OLT group (B group). Besides general data, the surveillance of blood lactate, the number of failure organs, APACHE Ⅱand MODS were recorded. 28-days survival rate and follow-up were checked. Results The mortality of hospitalization in non-OLT group was 30% and 57.6% in OLT group. The level of blood lactate in OLT group at the 1 st day increased significantly, which was statistically differ-ent with that in non-OLT group (P <0.01). It decreased but kept higher than that in non-group in following seven days. The numbers of failure organs in OLT group were more than in non-OLT group (P <0.01). The continuous APACHE Ⅱ score had no significant difference between two groups. But the continuous MODS score in OLT group was higher than in non-OLT group (P <0.01). Conclusions The 28-days mortality of OLT with severe sepsis is almost two times as much as that of non-OLT. It should cause more attention. The OLT with se-vere sepsis is more likely suffered from failure organs and difficult to recovery. To assess the condition of failure organs in OLT patients with severe sepsis, MODS score is better than APACHE Ⅱ score in this study. It is suggested that the standard of score system could be improved or come up with new score for organ transplantation. It will be better if blood lactate score is included.
2.Roles of MTH1 gene in the occurrence and treatment of cancers
Journal of International Oncology 2016;43(1):26-28
MTH1 protein can mitigate DNA damage which caused by reactive oxygen species through hydrolyzing oxidized deoxy-ribonucleoside triphosphate.MTH1 is highly expressed in various cancers, so as to maintain the viability of cancer cells.MTH1 is closely related to tumor associated genes such as microRNA,RAS gene and p53 gene.Moreover, MTH1 inhibitors can selectively suppress the proliferation of cancers,which is a potential new target in tumor therapy.
3.Reoperation of postoperative recurrent hepatocellular carcinomia
Chinese Journal of General Surgery 1993;0(02):-
Objective To evaluate the curative effect of reoperation on postoperative recurrent hepatocellular carcinoma (HCC). Method From Jan.1995 to Dec. 1998, 18 recurrent HCC patients underwent 27 sessions of reoperation in our hospital. Result The mean interval between first and second operation among 18 patients was 39?14 months, and for the 9 cases, in which cancer recurred after second surgery, the mean interval between the second and the third operations was 21?4.5 months (P
4.Significance and importance of using micronucleus frequencies in peripheral blood lymphocytes as a biomarker for assessing early health of workers exposed to polycyclic aromatic hydrocarbon.
Xiao ZHANG ; Lei GUAN ; Kun HUANG
Chinese Journal of Industrial Hygiene and Occupational Diseases 2012;30(12):968-971
5.Relativity between Deviation of Nasal Septum and Acute Sinusitis
Xiangyu LOU ; Ming GUAN ; Shuixian HUANG
Journal of Zhejiang Chinese Medical University 2013;(10):1211-1212
[Objective] To discuss the relativity between the deviation of nasal spetum and acute sinusitis ’offering relative theoretic base for clinical perven-tion, causa morbi, diagnosis and treatment.[Method] Select 1 case of deviation of nasal spetum from Hangzhou City First People Hospital, make pair com-parison 2 sides of the sinus, record the attack of 2 flanks of sinus with deviation of septum(frontal sinus, maxil ary sinus, frontal and back ethmoidal cellules, sphenoid sinus) for the sinusitis, and make quantitative analysis to the severity, evalulate susceptivity and severity. [Result] There ’s no difference of statistical meaning on the occurrence rate and sinusitis scores for the 2 flanks of the patient.[Conclusion]For such patient of acute sinusitis, the susceptivity and severi-ty are the same for 2 flanks of nasal cavity; the pure correction of the deviation can ’t effectively prevent or treat acute sinusitis; for such patient, we shal correspondingly evaluate and treat the compensatory change caused by deviated spetum.
6.Case-based study (CBS) in combination with reference-induced self education (RISE) in clini-cal teaching of digestive endoscope diagnostics
Weili HUANG ; Xiaohui GUAN ; Baorong CHI
Chinese Journal of Medical Education Research 2015;14(10):1044-1048
Objective To explore the effect of applying Case-based study (CBS) in combina-tion with Reference-induced self education (RISE) in clinical teaching of Digestive endoscope diag-nostics. Method One hundred and twenty undergraduates in Grade 2012 of clinical imaging speciality of Beihua University were selected as the objects of study and classified at random into experiment group (60 students) and control group (60 students), In the course of teaching, the traditional LBL study and CBS-RISE study were separately employed, and examined through theoretical assessment in combination with case analysis, and the teaching effect in experiment group students was also evaluated through examination paper. SPSS 16.0 statistic soft ware was employed for data treatment and analysis, t test was used for quantity data and expressed as x±s, and x2 test for number counting data, and P<0.05 as the statistical significant difference. Results In experiment group, the total result, and the results of theoretical examination and case analysis were all superior to the control group, and the dif-ference between two groups exhibited statistical significance (P values respectively 0.008, 0.017 and 0.021). The excellent and good rate of the experimental group's theory examination score was 70%, which was higher than that of the control group (48.3%). The excellent and good rate of the experimen-tal group in the case analysis of examination results was 63.3%, higher than that of the control group (43.3%), and the difference was statistically significant (P values respectively 0.016 and 0.028). The students in experimental group showed better degree of satisfaction to the teaching model of CBS-RISE. In general, they considered that the CBS-RISE model could stimulate the learning interest of students, enhance the ability of analyzing and solving problems, deepen the understanding of knowl-edge, train a better clinical thinking model, and also develop a cooperative group idea. Conclusions CBS-RISE teaching model is feasible in the application of teaching in digestive endoscope diagnostics, and beneficial to the training of self learning ability and clinical consideration, deserving populariza-tion.
7.Expression of Bcl-2, integrin ?3 protein in resected non-small cell lung cancer and its clinical relevance
Wei WANG ; Huiqiang HUANG ; Zhongzhe GUAN
China Oncology 2001;0(03):-
Purpose:To study the expressions and its clinical significances of bcl-2 and integrin?3 in human Non-small cell lung cancer(NSCLC)after radical surgical resection. Methods:Immunohistochemical (IHC) staining of bcl-2 and integrin?3 was performed on paraffin-embedded sections from 269 NSCLC patients who underwent surgery and were followed up for 1.1 to 122.2(median, 48.4) months postoperatively. Differences in survival rates and clinical characteristics were evaluated by SPSS10.0 statistical software packet. Results:The prevalence of bcl-2, integrin?3 and both these proteins expression in NSCLCs was 56.9%, 47.2% and 34.9%, respectively. Bcl-2 expression was seen more frequently in female NSCLCs and smaller tumors. Integrin?3 was seen more frequently in squamous carcinomas. Cox multivariate analysis showed that bcl-2 expression in stage Ⅱ NSCLC was an independent risk factor (5 years survival rate: 39.3% vs 52.1% P=0.04), and both bcl-2 and integrin ?3 positive in stage Ⅲa NSCLC are independent good factors(5 years survival rate: 34.5% vs 23.8% P=0.04). Conclusions:Bcl-2 and integrin ?3 are indicators of NSCLC biological behavior. Combined analysis of expression of these proteins in tumors may indicate the prognosis of NSCLC.
8.Comparison of electroretinograms between preterm and full-term infants
Tianqin GUAN ; Yanjuan SHENG ; Shizhou HUANG
Chinese Journal of Ocular Fundus Diseases 2003;0(05):-
Objective To investigate the degree of retinal development in preterm infants. Methods Flash electroretinography (ERG) was performed on 25 healthy preterm infants and 25 full-term ones, and the response of rod cells and cone cells and maximal mixed responses were recorded. The delitescence and amplitudes of a- and b-waves and the ratio of amplitudes of b-/a-wave of maximal responses were analyzed. Results Compared with the full-term infants, The delitescence of responses of rod cells in preterm infants was statistically longer (.t=11.007,P.=0.000) but without any significant changes of amplitudes (.t=1.836,P.=0.069); statistically longer delitescence (.t=2.44, P=0.010; (t=10.800,)P.=0.000) and lower amplitude (.t=5.804,P=0.000; t=5.809,P.=0.000) of a- and b-wave of maximal response were found in preterm infants group. In the response of cone cells, there were significant differences of the delitescence (.t=4.444,P.=0.000) and amplitude (.t=3.819,P.=0.000)of a-wave and delitescence of b-wave(.t=2.850,P.=0.005) between the two groups, and no statistical difference of amplitude of b-wave (.t=0.486,P.=0.628) between the two groups. The ratio of amplitudes of b-/a-wave of the maximal mixed response was not significantly different between the two groups ((.t=1.142,)P.=0.256). Conclusions The development of retinal function is slower in preterm infants than that in full-term ones.
9.Effects of Environmental Tobacco Smoke on Serum Lipid and the Lipases of Serum and Liver and Lung in Rats
Ming ZENG ; Ting HUANG ; Lan GUAN
Journal of Environment and Health 1992;0(04):-
0.05).The smoking group had a higher TG level and a lower HDL-C level than the control one with statistical significance(P0.05).The HL activities in serum,lung and liver of smoking group were lower than those of control group(P
10.PSD_(93) knockout decreased neurotoxity induced by pletelet activitaing factor
Yun XU ; Rong HUANG ; Dening GUAN
Journal of Clinical Neurology 1997;0(06):-
Objective To investigate whether PSD 93 reduce neurotoxity induced by platelet activating factor (PAF) and study on the mechanism of cell molecularbiology.Methods PAF treated the cultural cortex neurons in wild type and PSD 93 knockout mice and the cells were stained with PI/Calcein AM for apoptosis; neuronal viability was measured by MTT. The expression of PSD93 and PSD95 protein in neurons was tested by Western Blot.Results PSD 93 and PSD 95 expressed in wild type neurons and only PSD 95 expressed in PSD 93 knockout. Cortex neuronal apoptosis in PSD 93 knockout mice was lower then that in wild type ( P