BACKGROUND:Raloxifene is the third generation of selective estrogen receptor modulators, which can decrease bone loss, increase bone mineral content, and reduce fracture risk. OBJECTIVE: To study the effects of raloxifene combined with self-setting calcium phosphate cement on the repair of rabbit mandibular defects. METHODS:Totaly 36 New Zealand white rabbits were selected to prepare 8 mm×4 mm×3 mm mandibular defect models, and then randomized equaly into experimental group (raloxifene, 7.5 mg/kg per day, combined with self-setting calcium phosphate cement), drug group (raloxifene, 7.5 mg/kg per day), artificial bone group (self-setting calcium phosphate cement). Rabbits were sacrificed 4, 8 and 12 weeks later, respectively, for measurement of bone morphogenetic protein 2 using immunohistochemistry method and transforming growth factor β using a laser scanning confocal microscope. RESULTS AND CONCLUSION:After 4 and 8 weeks, the expression of bone morphogenetic protein 2 was obviously higher in the experimental group than the drug and artificial bone groups; after 12 weeks, bone remodeling was basicaly complete in the experimental group, and the expression of bone morphogenetic protein 2 became lower than that in the other two groups. The expression of transforming growth factor β in the experimental group was gradualy increased and reached the peak at 8 weeks, while in the drug and artificial bone groups, the expression of transforming growth factor β exhibited an increasing trend within 4-12 weeks, which was close to the peak. These findings suggest that raloxifene can promote early expression of bone morphogenetic proteins and early calus formation as wel as accelerate the repair of bone defects with calcium phosphate cement.

Objective To investigate analgesic effects of flurbiprofen in lower extremity liposuction for patients with primary lymphedema. Methods A total of 60 patients receiving lower extremity liposuction under general anesthesia were allocated to 3 groups:the control group (group A) received no analgesic drug 10-20 min before the end of operation, the parecoxib group (group B) received intravenous parecoxib 40 mg, and the flurbiprofen group (group C) received intravenous flurbiprofen 100 mg.The VAS was recorded at 1, 2, 6, 12, and 24 h after operation.Adverse reactions were also recorded . Results The VAS of rest pain and motion pain at 1, 2, 6, and 12 h were significantly lower in the group B than those in the group A (P<0.05);the VAS of rest pain and motion pain at 1, 2, and 12 h were significantly lower in the group C than those in the group A (P<0.05).The VAS at 1 and 2 h did not differ between the group B and C (P>0.05), but had significant difference at 6 and 12 h (P<0.05).No significant differences in the VAS at 24 h were observed among the three groups (P>0.05).Adverse reactions were not different among the three groups (P>0.05). Conclusion Both flurbiprofen and parecoxib sodium can achieve good postoperative analgesic effects in patients with lymphedema receiving lower extremity liposuction .

0.05).Conclusion The propofol TCI system can be safely used in surgical operation for patients with lymphedema.

Child ; Congresses as Topic ; Humans ; Nephrology ; Pediatrics

International Cooperation ; Nephrology ; trends ; Pediatrics ; trends

[ ABSTRACT] AIM:To study the effects of nuclear factor kappa B ( NF-κB) on human hepcidin expression in fer-ric ammonium citrate ( FAC)-induced HH4 hepatocytes.METHODS:Non-transformed HH4 cells were exposed to FAC at concentrations of 0.1, 1, 5 and 10 mmol/L for 48 h.The expression of iron regulatory gene hepcidin was determined by semi-quantitative RT-PCR.The effects of NF-κB on hepcidin transcriptional activity were detected using chromatin immuno-precipitation (ChIP), electrophoretic mobility shift assay (EMSA) and dual-luciferase reporter assay system, combined with the inhibition experiments of intracellular NF-κB activity.RESULTS: FAC at concentrations of 5 mmol/L and 10 mmol/L significantly enhanced the expression of hepcidin.The results of ChIP and EMSA showed the binding of NF-κB to the upstream of hepcidin promoter.Treatment with NF-κB inhibitor BAY 11-7082 attenuated hepcidin expression.The lucif-erase activity in the cells transfected with recombinant luciferase reporter plasmid was obviously higher than that in control group.CONCLUSION:NF-κB is the transcription factor that contributes to hepcidin expression in iron overload-induced HH4 cells.

Objective To present the management of increasing the closure stress of urethra for treatment of stress urinary incontinence in women. Methods In 12 patients with stress incontinence the posterior urethra and the anterior wall of the bladder was incised.Then the wall of posterior urethra,bladder neck and bladder trigone were trimmed and sutured to form a tube whereby to elongate and tighten the posterior urethra referring to Campbell-Young's way and according to Laplace's law. Results The mean period of post-operative follow-up was 8.8 years.Eleven patients could completely control their urinating without residual urine after the operation.The short-term and long-term outcomes were the same in these 11 patients.For the remaining one patient little urine was spilt when the abdominal pressure was increased with exertion. Conclusions Elongating and tightening the posterior urethra is simple,effective and safe for treatment of stress urinary incontinence in women.

Alzheimer’ s disease ( AD) is one of the most common dementia of neurodegenerative disorders, which results from the deposition of amyloid-beta ( Aβ) and there are no curative treatments for this disease at present.It had been proved that prion protein is the receptor for Aβand it plays a key role in the progress of AD with dual-side effects. Prion protein can not only transfer neurotoxicity to neurons but also protect them from neurotoxicity of Aβ.The polymor-phisms of prion protein encoding gene ( PRNP) affect the AD incubation period and clinical symptoms in humans and other animals.The discovery of PRNP mutational mouse fills the gaps of existing AD mouse models in this research area, which is potential for the studies of pathogenesis, new drugs design and testing aspects.The role and effects of prion protein in AD pathogenesis were summarized in this paper, furthermore, the discovery and utility of PRNP gene mutational mouse in research on AD and/or amyloid diseases were reviewed, and in order to provide some guidance for AD animal model study.

The proteomics definition, investigation method and its a pplication in cancer research were simply introduced. Proteomic research is to r eveal the function of genes from an integrated, kinetic and quantitative view at the global protein level, which is an important component of post-genome proje ct. Cancer is a kind of complex disease involved by multi-genes. Proteomic rese arch will be helpful to discover the mechanism of cancer development, to find sp ecial malignant tumor markers and targets of drug treatment.

Aim To study the inhibition of genistein on proliferation and transcription of c-fos mRNA in human umbilical vascular smooth muscle cells(hUVSMC) induced by monocyte chemotactic protein-1(MCP-1). Methods Growth-arrested hUVSMC were stimulated with MCP-1(10 ?g?L-1) prior to co-treatment with different concentrations of genistein (10,30,90 ?mol?L-1). The response of hUVMSC to these treatments was observed in comparison with that of control group. The proliferation of hUVMSC was evaluated by cell counting. The expression of c-fos mRNA was detected by RT-PCR. Results Low concentration of genistein(10 ?mol?L-1) inhibited the proliferation of hUVSMC and high concentration of genistein(30,90 ?mol?L-1) inhibited the expression of c-fos in hUVSMC induced by MCP-1. Conclusions Genistein could suppress the proliferation of hUVSMC induced by of MCP-1. Its mechanisms may involve the down-regulation of c-fos mRNA expression.