The measures taken by Library of Southern Medical University to improve the use of its electronic re-sources were described according to the major problems in use of electronic resources in domestic college and uni-versity libraries from resources assessment, resources integration and revealing, resources popularization and librarians support, with certain rational suggestions proposed for increasing the utilization ratio of electronic resources, inclu-ding popularization of resources, training of user information literacy, and improvement of users ability.

Application of TMS technology in newborn screening has resulted in major expansion of disorder panel for metabolic diseases in recent years. This automated, multiplex testing methodology detects multiple analytes from single analysis of one blood spot, which leads to detection of 30-35 disorders of amino acids, organic acids, and fatty acids metabolism. The early identification of persons affected with inborn errors of metabolism has led to unexpected discoveries related to the natural history of the disorder or options for therapy. This article summarized (1) the basic principles of this technology and methodology. (2) Current status of application of this methodology in the United States, European countries and in China. (3) The positive impacts on the public health and advances in medical genetics. Finally (4) Challenges, issues and possible solutions. The purpose of this article aimed at introducing new technology and exploring the possibilities of implementing into developing countries where medical genetics is not developed and foreseeing the possible problems and obstacles.

T-cell non-Hodgkin lymphoma is a group disease with unique clinical and pathological features. It is more aggressive and has lower chemotherapy sensitivity than B-cell lymphoma. In recent years, to search for effective treatment, novel agents such as gemcitabine, targeted therapy combined with chemotherapy and high-dose chemotherapy combined with hematopoietic stem cell transplantation are applied in the treatmentof T-cell non-Hodgkin lymphoma. And some progresses are achieved.

Objective　To define the rational extent of radiation portal in adult patients with early-stage Hodgkin's disease (HD).Methods　From Jan 1984 to Dec 1997,206 adult(≥15 year-old) HD patients with stage Ⅰ～Ⅱ lesions were admitted into the cancer hospital of Chimese Academy of Medical Sciences. 130 cases received radiotherapy alone (RT group). 76 cases radiotherapy combined with chemotherapy (R+C group). Involved-field (IF) was used in 7 patients (3.4%),mantle-field(MF) in 34 (16.5%) patients,subtotal nodal irradiation (STNI) in 140 (68.0%) and total nodal irradiation (TNI) in 25 (12.1%) patients. Kaplan-Meier method was used in survival analysis and log rank test for comparson.Results　①The overall 5-,10-year accumulated survival rates (ASR) were 85.1% and 73.2%. The 5-,10-year disease free survival rates (DFSR) were 68.0% and 63.6%, respectively.② The 5-year ASRs in the MF,STNI and TNI groups were 69.2%,93.3% and 94.4%,respectively (P<0.05)；the 5-year DFSRs in these respective groups were 54.2%,79.2% and 79.9% (P<0.05).③ In R+C patients ,the 5-ASRs in the IF/MF and STNI/TNI groups were 75.7% and 90.6%(P<0.05); 5-year DFSRs were 43.1% and 73.3%(P<0.05).Conclusions　The stage ⅠA patients with favouable prognostic factors can be cured by using mentle-field only,but patients in the other early-stages should be given subtotal nodal irradiatiion when radiotherapy is given alone. It is suggested that patients with unfavorable prognostic factors should receive radiotherapy combined with chemotherapy.

Objective To establish a method for simultaneous determination of 6 kinds of organophosphorus pesticides in water.Methods The liquid-liquid extraction,gas chromatography with RTX-50 capillary column and flame photometric detector were used to determine 6 kinds of organophosphorus pesticides(DDVP,dimethoate,malathion,methyl parathion,parathion,chlorpyrifos)in water.Results Chromatographic resolutions for the above 6 kinds of organophosphorus pesticides were both over 1.5.The linear range was 2.0-16.0 ?g/ml(r=0.999 2～0.999 8),the detection limits were 0.009 2～0.076 ng,RSDs were 1.5%-7.1%,the average recovery rates were 93.0%-98.7%.6 kinds of organophosphorus pesticides could be absolutely separated on RTX-50 capillary column.Conclusion This method presents an advantage in detection limit,precision and accuracy compared with that in GB13192-1991,it will meet the requirement of Sanitary Standard for Drinking Water and routine water quality analysis.

Objective: To observe the role of nutritional support in treatment of chylous leakage after thyroid carcinoma operation.Methods: The parenteral nutrition combined with somatostatin and re-operation was used in treatment of 2 cases of chylous leakage after thyroid carcinoma operation.Fluid volume from neck drainage catheter and plasma albumin concentration were determined in 2 cases.Results: Fluid volume from neck drainage catheter rapidly reduced in 2 cases after using somatostatin and parenteral nutrition.1case was cured after conservative treatment.1 case was cured after re-operation combined with nutrition support.Conclusion: By using nutrition support,fluid volume from neck drainage catheter can be obviously reduced and serum albumin or nutritional state improved in chylous leakage patients after thyroid carcinoma operation.

Objective To establish a HPLC method to detect the propofol concentration of plasma in children undergoing cardiopulmonary bypass. Methods RP-HPLC with fluorescence detector was used in the study. Plasma samples were precipitated by the thymol-methanol solution, and centrifugated. 20?l of supernatant fluid was injected for analysis and separated by Dikma Diamonsil C18 column. The mobile phase was composed of acetonitril:water =56:44(V/V), flo rate was 1.0ml/min. Fluorescence detective wave lengths were ?ex= 276nm, ?em=312nm. HPLC machine was Agilent1100 series. Main column was Diamonsil C18(150?4.6nm,5um),guard column was Phenomenex Security(4mm?3mm). Results There was a good linear relationship within the range of 0.02～8.0mg/L(r=0.9993, n =9),the limit of detection was 5?g/L,average recovery was 96.99%,intra RSD(relative standard deviation )was between0.810% and 5.73%,inter RSD was between 0.932% and 7.337%. Conclusions The method is sensitive, simple, rapid, good linear relationship and precise. The experiment can be used for determination of propofol concentration in child undergoing cardiopulmonary bypass.

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Objective:To explore the clinical diagnosis,therapy and pathologic characteristics of primary bilateral breast cancer in order to improve the survival of breast cancer patients.Methods:Eleven cases of primary bilateral breast cancer patients were retrospective analysised.Results:Among the 11 cases of primary bilateral breast cancer patients,2 cases were dead for multi organ metastasis;9 cases survived.Among which one has been alive for 6 years after operation.Conclusion:The early diagnosis and treatment of the second primary cancer remain the key factor to improve the outcome of the bilateral breast cancer patients.

Objective To find antigens related with hepatocellular carcinoma(HCC).Methods Human Zinc Finger Protein 216(ZNF216) was identified by SEREX(serological analysis of recombinant cDNA expression libraries,SEREX).The 6His fusion protein of ZNF216 was expressed by bacteria and purified with a nickel affinity chromatography column.The level of anti-ZNF216 antibody in the sera was detected by ELISA,andZNF216 mRNA level in HCC tissue and its peripheral normal tissue was detected by RT-PCR.Results The level of anti-ZNF216 antibody in the sera of HCC patients was higher than that of healthy individuals.ZNF216 mRNA level in HCC tissue is evidently higher than peripheral normal tissue.Conclusion The over-expression of ZNF216 in the HCC sera and tissue indicates that ZNF216 perhaps plays a role in HCC.