Objective To evaluate the application of the spleen-preservation technique in splenic injury.Methods The clinical data of 208 cases with splenic trauma were analysed retrospectively.There were,20 cases treated with non-surgical therapy;20 cases treated with simple splenorrhaphy,48 cases with ligation of splenic artery,splenorrhaphy and/or partial splenectomy,20 treated with splenectomy and autologous spleen transplantation,and 100 cases with simple splenectomy.Results In 108 splenic preservation cases,who were operated or non-operated(SP group),the value of lgM and CD3 was significantly higher than that in the splenectomy group(P

Objective:To design automatic reverse osmosis water system with new design idea of reverse osmosis water system, to make sure of the procedure. Methods:During the design, using microcomputer technology to control water preparation and disinfection. Results:Using MCU to control preparation of water and disinfection, in order to monitor water quality. If water quality exceeds the standard, the system will alarm and generate records. Conclusion: Using microcomputer technology to design Reverse osmosis water system, not only reducing artificial, but also to improve the safety and controllability, even the quality control of reverse osmosis water system.

This paper is to prepare curcumin (Cur) loaded mesoporous silica nanoparticle (Cur-MSN), evaluate its release behavior and anti-cancer activity in vitro. Mesoporous silica nanoparticle (MSN) was prepared by polymerization method and Cur-MSN was obtained using solvent evaporation method and impregnation centrifugation method. The preparation method was optimized using entrapment efficiency (EE) and loading efficiency (LE) as indexes. Cur-MSN was characterized with scanning electron microscope and its particle size and zeta potential were determined. Finally, in vitro release behavior in 0.2% SDS solution and its cell-killing effect on HeLa cells were also evaluated. The Cur-MSN prepared with process optimization method was round and uniform and exhibited typical mesoporous characterization. The mean particle size and Zeta potential of Cur-MSN were 75.8 nm and -30.1 mV, respectively. EE and LE of three batches of Cur-MSN were (72.55 ± 2.01)% and (16.21 ± 1.12)%, respectively. In vitro release behavior of Cur-MSN showed a sustained release profile with 83.5% cumulative release within 96 h. The killing effect of Cur-MSN on HeLa cells was dose-dependent with IC50 of 19.40 mg x L(-1), which was similar to that of Cur.
Antineoplastic Agents, Phytogenic ; chemistry ; pharmacology ; Curcumin ; chemistry ; pharmacology ; Drug Carriers ; chemistry ; Drugs, Chinese Herbal ; chemistry ; pharmacology ; Humans ; Nanoparticles ; chemistry ; Neoplasms ; drug therapy ; Particle Size ; Porosity ; Silicon Dioxide ; chemistry

The clinical application of GlucoWatch used in 32 Chinese diabetic patients was evaluated to determine its value in following blood glucose trends and track patterns and to know the compliance of patient. The results confirmed the accuracy of GlucoWatch and showed that it could follow blood glucose trends and track patterns. The skin irritation was mild and most subjects thought that blood glucose monitoring by GlucoWatch could reduce the pain caused by traditional methods.

Objective To discuss the features of MRI and molybdenum target X-ray in mammary primary non-Hodgkin's lymphoma.Methods 3 cases of mammary primary non-Hodgkin's lymphoma were studied.The features of MRI and molybdenum target X-ray werecollected and analyzed(all 3 cases had molybdenum target X-ray in MLO site and CC site,one had MRI image).Results All 3 caseslesions were in single side,one case had two lesions in one side.The diameter of all the lesions were more than 3cm.The lesion presented as iso-or slight hyperdensity shadow,and related to breast tissue and density was hemogeneous.The tumor was irregular but no bristle andcalcification.There was no skin and nipple involved in superficial of mammary gland.In T_1WI showed even low signal.In T_2WI showedeven little high or equal signal.Intensity was hemogeneous and obvious.The curve of time-signal intensity was platform pattern.Conclusion The features of MRI and molybdenum target X-ray in mammary primary non-Hodgkin's lymphoma are characteristic and helpful for deciding correct therapy plan.

Objective Congenital dislocation of the patella is rare and produces varying degrees of knee disability with the growth of the child. It should be managed as soon as the diagnosis is made. The purpose of this paper is to describe the procedure and to assess the results of congenital dislocation of patella treated with medial transfer of patellar tendon and lateral transfer of medial fascio muscular flap. Methods Five children with congenital dislocation of patella were treated with medial transfer of patellar tendon and lateral transfer of medial fascio muscular flap between June 1994 and June 1998. There were two boys and three girls. The age of the patients at the surgery varied from 4 to 10 years. Four of them were unilateral and one was bilateral without any other deformities. An S-shaped incision is made to expose the distended medial fascio muscular flap, dislocated patella and well developed vastus medialis. The patellar tendon is detached from its insertion and released from the surrounding retinacula. A hole corresponding to the width of the patella is made in the membrane and another one is made through the synovial membrane and the fibrous capsule. The patellar tendon was sutured into the hole in the tibial metaphysis through the two membrane holes. The iliotibial band was lengthened and the biceps tendon was turned over the abundant medial retinaculum with a part of the vastus medialis to the lateral side of the synovial membrane. The patient was kept in a long leg cast and immobilized for 6 weeks. Results The average period of follow up was three years(range, one year and six months to five years). Four cases were able to extend the knee completely and one case had minus ten degrees in extention. Knee valgus in two patients disappeared after operation and one had a lower patella, but the function of knee was normal without any symptoms and signs. There were no complications in this series. Conclusion The patients with congenital dislocation of patella which is permanent and irreducible must be interfered with operation. Five patients were successfully treated with medial transfer of patellar tendon and lateral transfer of medial fascio muscular flap with less invasive and no complications, therefor it is worthwhile to be recommended for the treatment of congenital dislocation of patella.

Objective To assess the value of echocardiography in guiding surgical operation approach of ventricular septal defect (VSD). Methods A total of 200 patients with VSD underwent surgical repair. The type of VSD was determined with echocardiography before operation and the results were analyzed in comparison with operation approach. Results There was significant accordance in the type of VSD detected with echocardiography and the surgical findings, and the coincidence rate of 80.00%. The transatrial approach was chosen in patients with perimembranous VSD, subseptal cusp VSD and simple membranous;the anspulmonary approach was chosen in subarterial VSD, while transventricular approach was chosen in muscular VSD or VSD with large size. Combined approach was suitable in VSD complicated with intracardiac malformations. Conclusion The type of VSD determined with echocardiography plays a key role in the selection of operation approach, and is benefit to reducing complications.

Objective To know the advantages and disadvantages of problem-based learning from students'angle. Methods Interviewed 15 students by PBL scale and face to face interview,to know their ideas about problem-based learning,and then analyzed the results. Results PBL contributed to developing problem-solving skills,self-directed learning,team collaboration and extending professional knowl-edge. But it failed to show positive effect on students'learning motivation. Conclusions PBL has two sides on the outcomes of learning. Implementing PBL appropriately could gain the optimal effects.

Objective To explore a new way to improve the repairing effect of tendon allograft by pretreatment with ethyldimethylaminopropyl carbodiimide (EDC) cross-linking. Methods Rabbits' tendon allografts were obtained by lyopyilization.The allografts were then cross-linked and heparinized by EDC and N-hydroxy-succinimide (NHS) respectively.The degradation rate,cytotoxicity and histocompatibility of the cross-linked allografts were detected and compared with those of untreated at different time points.The injured anterior cruciate ligaments (ACL) were repaired by tendon allografts with and without cross-linking pretreatment in rabbits.The tendon-bone healing was observed and compared by light microscopy and scanning electron microscopy (SEM) at 1,3,6 months. Results The degradation rate of the pretreated tendon allografts [(6.26 ± 3.16)％] was significantly lower than that of the unpretreated [(30.70 ± 10.24)％]( t = 14.200,P = 0.025 ).The pretreated tendon allografts were atoxigenic and produced significantly lower inflammatory reaction ( P ＜ 0.05 ).The pretreated tendon allografts also showed more powerful capability of repairing ACL injury and shorter time of tendon-bone healing. Conclusion Tendon allografts cross-linked and heparinized by EDC/NHS may promote tendon-bone healing with improved stability,degradation and biocompatibility.

Objective　To explore the relationship between te lomerase activity and bile duct carcinoma and its significance in clinical diag nosis. Methods　Telomerase activities were examined in 23 cases of bile duct carcinoma, 5 cases of carcinoma adjacent tissues and 5 cases of nor mal bile duct tissues respectively with telomerase PCR-ELISA method. Results　Telomerase activity was detected in 18 of 23 cases (78.3%) of b ile duct carcinoma, and was not found in 5 cases of carcinoma adjacent tissues a nd 5 cases of normal bile duct tissues. The detection rate of telomerase activit y had no correlation with patients' age, sex, tumor site and size but significan tly correlated to metastasis of tumor (P<0.05). Conclusion　The level of telomerase activity was significantly higher in bile duct carcino ma and may be served as one of the clinical marker for malignant neoplasm becaus e of its spsecificity.