No abstract available.
Dermatitis* ; Granulocytes*

No abstract available.
Agranulocytosis* ; Granulocytes* ; Humans

No abstract available.
Drug Therapy* ; Granulocytes* ; Humans*

83 burn patients with the syndrome of bacterial infection and intoxication associated with leucopenia were divided in to 2 groups, the one of 32 patients without transfusion of white blood granulocyte, the other 51 patients with a transfusion rich with neutrophile leucocyte. Results: In the group of the syndrome of severe bacterial infection and intoxication associated with leucopenia, 39.2% have had complication, less than no transfusion group (68.7%). Mortality of the transfusion group: 82.4%, is higher than no transfusion group: 40.6%
Burns ; Bacterial Infections ; Granulocytes

No abstract available.
Drug Therapy* ; Granulocytes* ; Humans* ; Lung* ; Neutropenia*

We found an unusual morphology of eosinophil nucleus having longer chromatin filament in addition to a single narrow chromatin bridge. The nucleus having two chromatin filament bridge looked like two legged eosinophil, instead of usual glasses shape. As the physiologic function of the nucleus of granulocyte segmentation and the mechanism by which the lobes are formed during differention is still unknown, we could not know the definite nature and significance of these double chromatin filament. However we could suggest that they may be a reactive change of eosinophilia. This not uncommon morphology has not been described as yet. Here we report three cases of unusual morphology of eosinophil nucleus presenting double chromatin filament bridge, one case with a band form nucleus looked like ring shape, with brief review of literatures.
Chromatin* ; Eosinophilia ; Eosinophils* ; Eyeglasses ; Glass ; Granulocytes ; Leg

No abstract available.
Agranulocytosis* ; Granulocyte Colony-Stimulating Factor* ; Granulocytes* ; Humans*

INTRODUCTION: The ABX Pentra DX 120 (Pentra DX 120, ABX Diagnostics, Montpellier, France) adopted new technologies to perform differential leukocyte and erythroblast counts. The double matrix can discriminate Large Immature Cell (LIC), Immature Granulocyte (IMG), Immature Monocyte (IMM), Immature Lymphocyte (IML), and Atypical lymphocyte (ALY) in addition to a routine 5-differential count. For erythroblast (ERB), a fluorescence method is employed. In this study, we evaluated the performance of the Pentra DX 120 in the performance of differential leukocyte and erythroblast counts. METHODS: Precision was evaluated using 3-level control materials. Comparison analysis was performed on 200 samples: 100 normal and 100 abnormal samples. We evaluated the 5 part differential count, LIC, IMG, IMM, IML, ALY, and ERB. These parameters were analyzed in comparison with the results from the reference method, manual differential count. RESULTS: The coefficients of variation (CVs) of precision were <5% for neutrophils and lymphocytes, <15% for eosinophils and basophils and <7% for erythroblasts. The correlation coefficients (r) were >0.9 except monocytes and basophils. IMG, ALY and erythroblasts were also well correlated with manual count (r=0.8315, 0.5602, 0.8144, respectively). The efficiency of flagging system was 84% for LIC, 80% for ALY, and 78.0% for increased ERB (>2/100WBCs). CONCLUSIONS: The Pentra DX 120 performed reliable differential leukocyte and IMG, ALY, and ERB results demonstrated comparable performance to manual count. And, the flagging system was efficient for detecting each abnormal cell population. We expect the Pentra DX 120 double matrix and erythroblast count can reduce microscopic review rate in routine laboratory and promote laboratory efficiency.
Basophils ; Eosinophils ; Erythroblasts ; Fluorescence ; Granulocytes ; Leukocytes ; Lymphocytes ; Monocytes ; Neutrophils

BACKGROUND: We evaluated the performance of leukocyte differential counting and clinical usefulness of the morphologic flags of the SE-000, and set optimal criteria for selecting and reviewing the specimens with increased abnormal cells. METHODS: From the results of SE-000 and manual leukocyte differential counting in 100 healthy control and 520 patient specimens we evaluated the correlations on the leukocyte fractions as well as the frequency, sensitivity and false positivity of the flags. After determination of the review criteria we calculated total review rate from the 3,403 consecutive CBC specimens. RESULTS: In both control and patient groups the correlation between two methods was high with the exception of monocytes and basophils. Regarding the morphologic flags, Blast was sensitive (86.9%) however could not detect mature looking lymphoblasts. Immature granulocyte showed high sensitivity (93.7%). Left shift showed the highest frequency (34.6%) and false positive rate (82.8%). Atypical lymphocytes and NRBC showed relatively low sensitivity (63.6%, and 50.5%, respectively). We determined to review the slide when 1) All morphologic flags except Left shift are marked, 2) WBC <3,000/microliter or >20,000/microliter, Hb <8.0 g/dL or 18.0 g/dL, Platelet <100,000/microliter or >600,000/microliter, 3) Severe deviation of leukocyte fractions or 4) Specially requested by physician. As a result, total review rate was 25.0% while 14 abnormal cases with no flags could be additionally detected. CONCLUSIONS: A new review criteria determined from the results of CBC and leukocyte differential together with morphologic flags could reduce the review rate without skipping the abnormal cases.
Basophils ; Blood Platelets ; Granulocytes ; Humans ; Leukocytes ; Lymphocytes ; Monocytes

BACKGROUND: We evaluated the performance of leukocyte differential counting and clinical usefulness of the morphologic flags of the SE-000, and set optimal criteria for selecting and reviewing the specimens with increased abnormal cells. METHODS: From the results of SE-000 and manual leukocyte differential counting in 100 healthy control and 520 patient specimens we evaluated the correlations on the leukocyte fractions as well as the frequency, sensitivity and false positivity of the flags. After determination of the review criteria we calculated total review rate from the 3,403 consecutive CBC specimens. RESULTS: In both control and patient groups the correlation between two methods was high with the exception of monocytes and basophils. Regarding the morphologic flags, Blast was sensitive (86.9%) however could not detect mature looking lymphoblasts. Immature granulocyte showed high sensitivity (93.7%). Left shift showed the highest frequency (34.6%) and false positive rate (82.8%). Atypical lymphocytes and NRBC showed relatively low sensitivity (63.6%, and 50.5%, respectively). We determined to review the slide when 1) All morphologic flags except Left shift are marked, 2) WBC <3,000/microliter or >20,000/microliter, Hb <8.0 g/dL or 18.0 g/dL, Platelet <100,000/microliter or >600,000/microliter, 3) Severe deviation of leukocyte fractions or 4) Specially requested by physician. As a result, total review rate was 25.0% while 14 abnormal cases with no flags could be additionally detected. CONCLUSIONS: A new review criteria determined from the results of CBC and leukocyte differential together with morphologic flags could reduce the review rate without skipping the abnormal cases.
Basophils ; Blood Platelets ; Granulocytes ; Humans ; Leukocytes ; Lymphocytes ; Monocytes