Objective To investigate the minimally invasive treatment of complex renal calculi nursing.Methods Retrospective analysis,from February 2009 to January 2011,50 regular channels percutaneous nephrolithotomy nephrostomy lithotomy patients(observation group)and from January 2007 to January 2009,the traditional opening of the regular 50 surgical treatment of complex renal calculi patients(control group)were selected,and length of stay,hospital costs,complications such as postoperative infection and other indicators were observed between the two groups.Results Successful rate of percutaneous nephrostomy large channel lithotomy in the observation group were 100％.Days in hospital(t =2.95,P ＜0.05),costs(t =11.68,P ＜0.05)and complication rate after operation(x2 =6.25,P ＜ 0.05)were significantly different between the two groups.Conclusions Comprehensive patient evaluation and preoperative psychological communication,close observation and meticulous condition postoperative care,help to promote patient recovery and improve the quality of care.

Objective To explore the nursing care for complications of children with complex renal calculus who receive minimally invnsive percutaneous nephrolithotomy lithotripsy treatment.Methods Children who received routine minimally invasive percutaneous nephrolithotomy hthotripsy treatment for complex renal calculus from February 2008 to April 29,2011 and 30 cases who received conventional open surgery from February 2005 to 2008 were divided into the observation group and the control group,respectively.Postoperative hospital stay,hospital costs and the incidence of postoperative complications were compared between the two groups.Results Hospital stay in observation group was(12.80 ± 2.60)d,and(18.25 ± 3.34)d in control group; there was statistically significant difference between the two groups(t =10.32,P ＜ 0.05).Hospital costs in observation group was(13 924.9 ± 340.3)yuan,and(15 510.4 ± 509.3)yuan in control group,the difference was obvious(t =13.20,P ＜ 0.05)between the two groups.the incidence of postoperative complications was 10.3％ and 40.0％ in observation group and in control group,respectively,there was significant difference(x2 =6.60,P ＜ 0.05).Conclusions Minimally invasive percutaneous nephrolithotomy was better than traditional open surgery in terms of dealing with children with complex stone stones,close observation,careful and meticulous care and more records,more communication can help reduce the number of hospital stay,hospital costs and the incidence of postoperative infectious complications,etc.It is good for the rehabilitation of patients and improvement of the quality of care.

Objective To observe the effect and the mechanisms of ferulic acid on radiationinduced damage of mice peripheral blood and bone marrow hematopoietic function.Methods Ninety-six mice were randomly divided into sham irradiation group,irradiation group,positive drug group and 10,30,90 mg·kg-1 ·d-1 ferulic acid group,16 mice per group.Mice were exposed to 3.5 Gy γ-rays 24 h after first drug taken.Then,mice were given drugs for 7 d after irradiation.White blood cells in peripheral blood of 10 mice per group were counted 2 d before irradiation and 3,7,10,15 and 22 days after irradiation.The bone narrow of the other six mice was taken to detect the micronuclei frequency of polychromatic erythrocyte,the hematopoietic progenitor cell colony formation capacity,Thbd and HMGB1 protein expressions in mice bone marrow on the seventh day after irradiation.Results Compared with the irradiation alone group,the treatment of mice with ferulic acid 90 mg· kg-1 · d-1 increased the number of white blood cells in peripheral blood at 3,10,15 and 22 d after irradiation (t =2.267,2.399,1.945,2.828,P ＜ 0.05).Treatment with mice with ferulic acid 90 mg· kg-1 · d 1 decreased the micronuclei rate of erythrocytes in irradiated bone marrow (t =4.013,P ＜ 0.05),increased the clone numbers of CFU-E,BFU-E and CFU-GM of hematopoietic progenitor cells (t =2.366,2.953,3.115,P ＜0.05),improved the relative expression of the Thbd protein in bone marrow and the HMGB1 protein in nuclear (t =17.75,23.39,P ＜ 0.01).Conclusions Ferulilc acid could protect the bone marrow hematopoietic of mice exposed to irradiation by regulating the expressions of Thbd and HMGB1 protein,and then accelerate the peripheral cells recovery.

Objective To study the effect of pre-hospital and in-hospital integrated rescuing pathway on group injury treatment in high-altitude areas.Methods According to pre-hospital and in-hospital integrated rescuing pathway,24 cases of group wounded were treated with field evaluation,triage category,emergency care measures,specialist nursing,monitoring during transfer,in-hospital first aid,processing during and after operation,and effect of clinical treatment.Results There were 7 cases of combined injury,12 cases of debridement surgery and 5 cases of damage control surgery in this group,with the successful rate of 100％.Conclusions rofessional pre-hospital and in-hospital integrated rescuing pathway in high-altitude areas can provide ordered rescuing for group wounded and shorten the time without treatment,which is an important measure to ensure fast and efficient treatment and to improve the quality of nursing.

OBJECTIVETo investigate the therapeutic effect of pentosan polysulfide sodium (PPS) on chronic non-bacterial prostatitis (CNP) in rats.

METHODSBased on Robinette's method, we established a CNP model in 80 male SD rats, aged 6 months and weighing 315 - 450 g, by castration followed by subcutaneous injection of estradiol at 0.25 mg / (kg x d) for 30 consecutive days. Then we randomly allocated the model rats into a placebo group (n = 40) and a PPS group (n = 40) to receive intragastric administration of normal saline and PPS, respectively. After 8 weeks of treatment, the pathological changes in the rat prostatic tissue were observed by HE staining.

RESULTSVaried degrees of chronic inflammation and inflammatory cell infiltration were seen in the prostatic tissues of both groups of rats before the treatment. The inflammation was significantly improved after the treatment in the PPS group but not in the placebo group.

CONCLUSIONPPS has some therapeutic effect on CNP in the rat, and its mechanism may be associated with the abilities of PPS to repair the damaged glycosaminoglycan layer and inhibit inflammation in the prostate.

Animals ; Chronic Disease ; Cystitis, Interstitial ; drug therapy ; Disease Models, Animal ; Male ; Pentosan Sulfuric Polyester ; therapeutic use ; Prostate ; pathology ; Prostatitis ; drug therapy ; pathology ; Rats ; Rats, Sprague-Dawley

Objective To formulate the evaluation criteria of percutaneous renal puncture patients ' health education,and explore the application effect on the perioperative care.Methods Used the Delphi method,through the three times of questionnaires consulting for 17 experts,the evaluation criteria of health education for percutaneous nephrolithotomy surgery patients was developed.Observation group (n =40) received the health education accordance to the evaluation criteria,while control group (n =40) received the conventional health education.Then,the knowledge awareness of the disease,satisfaction of patients and their families were observed.Results The evaluation criteria of health education for percutaneous nephrolithotomy surgical patients included three first-level indicators,and 26 second-level indicators.In the observation group,health knowledge score was (28.21 ±2.25) and the awareness rate was 85.0％,and that in the control group respectively was (23.04 ± 4.12) and 65.0％,and the difference between the two groups was statistically significant (t =2.84,x2 =4.27,P ＜0.05).and patients and their families ' satisfaction of the observation group was significantly higher than that of control group (95.0％ vs 80.0％,97.5％ vs 85.0％,x2 =4.67,4.35,respectively; P ＜ 0.05).Conclusions The evaluation criteria of health education for percutaneous nephrolithotomy surgical patients can provide evidence for the health education quality control,which is important for c-linical guidance.

Objective To construct a scientific,reasonable,effective index system for performance appraisal in the whole army urology care demonstration base.Methods The weight,reliability,construct validity and discriminant validity of each index were quantitatively analyzed and tested by literature data,theoretical analysis,a qualitative research method and Delphi (Delphi) expert consultation method,and the index system for performance appraisal was established in the whole army urology care demonstration base.Results The index system for performance appraisal was identified including level Ⅰ indicators 4 and level Ⅱ indicators 20 item pool in the whole army urology care demonstration base,Lanzhou General Hospital of Lanzhou Military Region.The quality of care,the operational capacity of nurses,nurses job satisfaction and patient satisfaction after the implementation of the performance appraisal were significantly higher than those before the implementation (P ＜ 0.05).Conclusions The index system for performance appraisal has good construct validity,internal continuity measurement reliability and discriminant validity.The research results is high reliability,and provides a basis for carrying out the effective performance management and controlling quality of care in the whole army urology care demonstration base.

Objective To study the role of Cx43 in X-ray induced apoptosis of HUVEC cells and its mechanism.Methods Flow cytometry was used to detect the apoptosis of HUVEC cells at 48-96 h after 10 Gy X-ray irradiation and at 72 h after irradiation of different doses.Western blot was used to detect the protein expressions of Cx43 and cleaved caspase-3 in HUVEC cells at 72 h after 0,5,10 and 20 Gy irradiation.Small interfering RNA was transfected into HUVEC cells to silence Cx43 expression,the Cx43 bearing plasmid was transfected into cells to overexpress Cx43.The effect of Cx43 knockdown or overexpression on apoptosis induction and cleaved caspase-3 protein expression were detected by flow cytometry and Western blot,respectively.Results The apoptosis of HUVEC increased significantly from 48 h to 96 h after X-ray irradiation and in a dose-dependent manner at 72 h after irradiation.The expression of Cx43 protein was negatively correlated with the dose but the expression of cleaved caspase-3 was positively correlated with the dose in the range of 0-20 Gy.After Cx43 silencing,the proportion of early apoptosis and apoptosis combined with dead cells were significantly higher than that of the siRNA control group(t =3.674,6.375,P ＜ 0.05).After Cx43 overexpression,the proportion of early apoptosis and apoptosis combined with dead cells were significantly lower than that of vector control group(t =9.399,11.190,P ＜ 0.05).The expression of cleaved caspase-3 in the Cx43 silencing group was higher than that in the siRNA control group,but this protein in the Cx43 overexpressed group was lower than that in the vector control group.Conclusions Cx43 may protect X-ray irradiated HUVEC cells from apoptosis by down-regulating the activation of caspase-3.

Objective:To investigate the changes of connexin 43 (Cx43) in human umbilical vein endothelial cells (HUVEC) after X-ray irradiation and its influence on the stiffness of irradiated cells.Methods:Western blot was used to detect the expression of Cx43 in HUVEC cells at different time points (0, 6, 12, 24 and 48 h) after different doses of X-ray irradiation (0, 2.5, 5, 10 and 20 Gy), and the phosphorylation levels of three phosphorylation sites (Ser279/282, Ser368 and Tyr265) of Cx43 at different time points (3, 6, 24 and 48 h) after 0, 5 and 10 Gy irradiation. The distribution of Cx43 protein in the irradiated HUVEC cells was detected by immunofluorescence. The stiffness changes of cells were detected by atomic force microscopy (AFM) at the depths of 50, 100 and 200 nm.Results:The expression of Cx43 in HUVEC cells was reduced at 6, 12, 24 and 48 h after 10 Gy X-ray irradiation( t=3.262, 3.708, 3.686, 6.825, P<0.05)and this decrease had a dose dependent manner at 24 h after 2.5, 5, 10 and 20 Gy irradiation ( t=3.034, 10.720, 13.130, 13.650, P<0.05). At 24 h after 5, 10 and 20 Gy X-ray irradiation, the distribution of Cx43 in HUVEC cells was transported from intercellular gap junctions to nucleus and perinuclear region. At 24-48 h after irradiation, the phosphorylation level of Ser368 at Cx43 increased and in a dose dependent manner. At 24 h after irradiation, the stiffness of the irradiated cells decreased significantly under the conditions of 100 and 200 nm ( t=3.362, 5.122, P<0.05), and recovered with overexpression of Cx43 ( t=2.674, 4.398, P<0.05). Conclusions:X-ray irradiation leads to the phosphorylation of Ser368 at Cx43, which promotes the degradation and nucleus/perinuclear translocation of Cx43 and reduces the stiffness of HUVEC. Increasing the expression level of Cx43 is helpful to the stiffness recovery of irradiated vascular endothelial cells, suggesting that Cx43 may be a potential target for regulating radiation injury of vascular endothelial cells.

OBJECTIVE: Pyroptosis is an inflammatory form of programmed cell death. This phenomenon has been recently reported to play an important role in radiation-induced normal tissue injury. Connexin43 (Cx43) is a gap junction protein that regulates cell growth and apoptosis. In this study, we investigated the effect of Cx43 on X-ray-induced pyroptosis in the human umbilical vein endothelial cells (HUVECs). METHODS: HUVECs, Cx43 overexpression, and Cx43 knockdown strains were irradiated with 10 Gy. Proteins were detected using western blot analysis. Cell pyroptosis was evaluated using the fluorescence-labeled inhibitor of caspase assay (FLICA) and propidium iodide staining through flow cytometry and confocal microscopy. Cell morphology and cytotoxicity were detected by scanning electron microscopy and lactate dehydrogenase release assay, respectively. RESULTS: Irradiation with 10 Gy X-ray induced pyroptosis in the HUVECs and reduced Cx43 expression. The pyroptosis in the HUVECs was significantly attenuated by overexpression of Cx43 as it decreased the level of active caspase-1. However, interference of Cx43 expression with siRNA significantly promoted pyroptosis by increasing the active caspase-1 level. Pannexin1 (Panx1), a gap junction protein regulates pyroptosis, and its cleaved form is used to evaluate channel opening and active state. The level of cleaved Panx1 in the HUVECs and Cx43 knockdown strains increased in the presence of X-ray, but decreased in the Cx43 overexpression strains. Furthermore, interference of Panx1 with siRNA alleviated the upregulation of pyroptosis caused by Cx43 knockdown. CONCLUSION Results suggest that single high-dose X-ray irradiation induces pyroptosis in the HUVECs. In addition, Cx43 regulates pyroptosis directly by activating caspase-1 or indirectly by cleaving Panx1.
Caspase 1 ; genetics ; metabolism ; Connexin 43 ; genetics ; metabolism ; Connexins ; genetics ; metabolism ; Gene Expression Regulation ; radiation effects ; Human Umbilical Vein Endothelial Cells ; physiology ; radiation effects ; Humans ; Nerve Tissue Proteins ; genetics ; metabolism ; Pyroptosis ; X-Rays ; adverse effects