In order to explore the application of the teaching mode based on WeChat official platform in the teaching of experimental pathology for international students, the students are required to browse the teaching objectives and the general pictures and microscopic pictures of experimental specimens on the public platform before the experimental class, and get in touch with the preliminary diagnosis of pathology at an early stage, contact with the preliminary diagnosis of pathology early, and consolidate and expand their knowledge after class by using the platform. The research shows that the mixed teaching mode based on the official platform can improve the diagnostic thinking of foreign students in pathology, deepen their understanding of pathology, increase the interaction between teachers and students and the supervision of teachers on students, and enhance their interest in learning.

Objective:To prepare encephalomyocarditis virus (EMCV) 3C protease antibody and detect response sites of these antibody.Methods:Prokaryotic expression vector pGEX-2T-3C was constructed and expressed. Then BALB/c mice was immunized with 3C protein and antibodies were obtained. The specificity of the antibody binding to 3C protein was detected by immunofluorescence and Western blotting. Three different fragments of EMCV 3C were expressed and detected with monoclonal antibodies through Western blotting.Results:The antibody reacted specifically with 3C proteins of prokaryotic expression, and bound well with 3C protein of BHK cells infected with EMCV virus through Western blotting and immunofluorescence. The reaction between fragments of 3C protein and the antibody revealed that the antibody could react with the C-terminal of 3C protein.Conclusions:The prepared monoclonal antibody recognized C terminal of EMCV 3C protein.