1.Development of cellular-I portable field anesthesia machine.
Jianhong DOU ; Gonghua ZHOU ; Chong SHI ; Yanwu ZHANG ; Weifeng TU
Chinese Journal of Medical Instrumentation 2010;34(5):347-349
OBJECTIVETo develop a portable field anesthesia machine system suitable for the medical first-aid on the spot.
METHODSThe three-dimensional structure of PFAM was designed with modeling software of Pro/E and manufactured according to the GB9706.29 and other national standards.
RESULTDue to its small footprint and very light weight, PFAM is completely portable and convenient on different occasions within or outside a hospital environment. It can support breathing of patients and delivery anesthetic gas, fitted for both adult and children patients. All of the safety alarm systems required are employed on board.
CONCLUSIONPFAM may play an important role in the first-aid in the field or outside the hospital.
Anesthesiology ; instrumentation ; Equipment Design ; Military Medicine ; instrumentation ; Monitoring, Ambulatory ; instrumentation ; Software
2.Effect of silicon dioxide nanoparticles on expression and DNA methylation of PARP-1 gene in HaCaT cells.
Chunmei GONG ; Linqing YANG ; Jichang ZHOU ; Gonghua TAO ; Xiaoli LIU ; Zhixiong ZHUANG
Chinese Journal of Industrial Hygiene and Occupational Diseases 2015;33(7):496-500
OBJECTIVETo study the effect of silicon dioxide nanoparticles on the expression and promoter region CpG islands methylation of (Poly [ADP-ribose] polymerase 1, PARP-1) gene in human HaCaT Cell.
METHODSHaCaT Cells were treated with nm-SiO₂at 0, 2.5, 5 and 10 µg/mL and micro-SiO₂at 10 µg/ml for 24 h and DAC treatment was given at 10 µg/ml group for 48 h. Real-time PCR and western blot assay was used to detect the expression of PARP-1 mRNA and protein. BSP (Bisulfite Pyrosequence, BSP) assay was used to detect the promoter region CpG islands methylation status of PARP-1 gene.
RESULTSAfter exposure to nano-SiO₂particles, compared to CTRL group, the mRNA and protein expression of PARP-1 in micro-SiO₂and 2.5 µg/ml group unchanged, but he mRNA and protein expression of PARP-1 in 5, 10 µg/ml as well as DAC group was down-regulated and there are statistical significance between CTRL group and 5, 10 µg/ml as well as DAC group and the PARP-1 promoter region CpG islands showed methylation.
CONCLUSIONnano-SiO₂can down-regulate PARP-1 expression in HaCaT Cell and this is associated with the change in the methylation of PARP-1 gene promoter region CpG islands induced by nano-SiO₂particles.
Cell Line, Tumor ; CpG Islands ; DNA Methylation ; Humans ; Nanoparticles ; adverse effects ; Poly (ADP-Ribose) Polymerase-1 ; Poly(ADP-ribose) Polymerases ; metabolism ; Promoter Regions, Genetic ; RNA, Messenger ; metabolism ; Silicon Dioxide ; adverse effects
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