Objective To label rat bone marrow mesenchymal stem cells with superparamagnetic iron oxide (SPIO) and to explore the tropism of BMSCs for hepatocellular carcinoma cells after transplantation in vivo. Methods BMSCs from bone marrow of Sprague-Dawly (SD) rats were cultured isolated and purified, Labeled BMSCs was achieved using Feridex. Twenty-four hepatocellular carcinoma models of SD rats were induced two weeks before tmnsplantation. The models were divided into three groups in random: the labeled BMSCs and unlabeled BMSCs were transplanted respectively into the rat's livers of experimental group (n = 12) and control group A(n =6) via spleens, and no transplant was done for control group B (n =6). MR imaging was performed to monitor the transplanted cells after 1,3,7,14 d using 1.5 T MR system. Signal intensity ratio (SI/SI*) between tumor and hepatic tissue on T_2 * WI were measured and compared by one-factor analysis of variance. After MR imaging, Prussian blue staining was performed. MR imaging findings were compared with histological sections. Results Prussian blue staining confirmed the labeling efficiency of BMSCs was above 90%. SI/SI* of experimental group before and 1, 3, 7, 14 d after transplantation were 3.18±0.21,1.98±0.20,2.38±0.28,2.70±0.25 and 3.16±0.24 respectively. Following transplantation of BMSCs, signal intensity decrease was found in hepatocellular carcinoma of experimental group(F =56.65,P <0.05) and low signal change decreased gradually and disappeared at two weeks after transplantation, while no remarkable low signal change was seen in the control group by T_2 * WI (P > 0.05). A large number of Prussian blue staining positive cells were found in hepatocellular carcinoma in experimental group. Histological section with Prussian blue staining had a good correlation with the signal intensity changes on MR images at different time. Conclusion BMSCs display significant tropism to hepatocellular carcinoma and may be an ideal gene therapy vehicle against hepatocellular carcinoma.

ObjectiveTo investigate the spiral CT and ultrasound findings of hepatic adenoma and the value of spiral CT and ultrasound in the diagnosis of hepatic adenoma. MethodsA retrospective analysis was performed for the spiral CT and ultrasound findings of 15 patients with pathologically confirmed hepatic adenoma in Langfang Hospital of Traditional Chinese Medicine from July 2009 to December 2015. ResultsAll the 15 patients showed hypoechoic, isoechoic, or hyperechoic ultrasound findings. Seven of these patients had single hepatic adenoma. In the 7 cases, 4 had low-density lesions, 1 had a slightly low-density lesion, and 2 had equal-density lesions; as for the arterial phase, 5 showed obvious enhancement, 1 showed moderate enhancement, and 1 showed mild enhancement; as for the portal venous phase and delayed phase, 3 showed reduced enhancement, 2 had equal-density lesions, and 2 showed gradual enhancement. Eight patients experienced multiple hepatic adenoma with 80 lesions in total. In the 80 lesions, 4 lesions (3 patients) showed a mixed density, 40 lesions (4 patients) showed a low density, 36 lesions (4 patients) showed a slightly low density; 8 lesions (4 patients) showed obvious enhancement in the arterial phase and reduced enhancement in the portal venous phase and delayed phase; 3 lesions (1 patient) showed obvious enhancement in the arterial phase and an equal density in the portal venous phase and delayed phase; 18 lesions (2 patients) showed obvious enhancement in the arterial phase and portal venous phase and reduced enhancement in the delayed phase; 49 lesions (5 patients) showed moderate enhancement in the arterial phase and reduced enhancement in the portal venous phase and delayed phase; 2 lesions (1 patient) showed no enhancement in any phase. The correct rates of spiral CT in the diagnosis of single hepatic adenoma and multiple hepatic adenomas were 143%(1/7) and 87.5%(7/8), respectively. ConclusionUltrasound can only suggest space-occupying lesions, but cannot analyze the lesions qualitatively; spiral CT with three-phase enhancement scanning has a great value in the diagnosis of hepatic adenoma, especially multiple hepatic adenoma.

Objective : To study the effect of hydroquinone on autophagy of human L-02 hepatocytes. Methods : L-02 cells were treated with different concentrations of hydroquinone(0,10,20,40 and 80 μmol/L) for 24 hours, the formation of autophagosomes was observed by transmission electron microscope;autophagosome formation was traced by mCheery-GFP-LC3B fusion protein. Western blot experiment was used to detect the expression of autophagy marker proteins LC3,Beclin-1,P62;immunofluorescence experiment was used to detect the subcellular localization and expression of autophagy proteins LC3,Beclin-1,P62. Results : Under the transmission electron microscope,it was observed that the autophagosomes and lysosomes of L-02 cells increased after the action of hydroquinone. Under the fluorescence microscope,the number of GFP-LC3B punctate yellow spots increased with the increase of hydroquinone dose,while the red flake spots decreased. Western blot results showed that hydroquinone increased the ratio of LC3Ⅱ/LC3Ⅰ protein in L-02 cells,and the difference between treatment groups and the control group was statistically significant(P<0. 05). Hydroquinone had no significant effect on Beclin-1 and P62 protein expression in L-02 cells(P>0. 05). The results of immunofluorescence experiments showed that LC3 and Beclin-1 were both expressed in the nucleus and cytoplasm of L-02 cells,hydroquinone had no effect on the subcellular localization of LC3 and Beclin-1 in L-02 cells,while 40 and 80 μmol/L hydroquinone could induce the translocation of P62 in L-02 cells from the cytoplasm to the nucleus. Conclusion Hydroquinone induces the increase of autophagosomes in L-02 cells,which may be related to the obstacles to autophagosome clearance.

Objective To establish bovine corneal opacity and permeability （BCOP） test， and determine its predictive ability for the eye irritation evaluation of cosmetics. Methods A total of ten reference chemicals were selected to establish the BCOP test. Then eye irritation of 16 routinely collected cosmetics in our laboratory was predicted. In vitro scores were calculated by the change in the opacity and sodium fluorescein permeability after exposure to the testing cosmetics， and subsequently compared with the historical data by Draize test. Results Reference chemicals with known irritation classification were correctly classified by the BCOP test， which was consistent with the classification of UN globally harmonized system of classification and labeling of chemicals. Moreover， the specificity of the BCOP test for the classification of non-irritating cosmetics samples was 80.0% （8/10）， and the sensitivity for weak to mild irritating cosmetics samples was 83.3% （5/6）. The BCOP test demonstrated an overall classification consistency of 81.3% （13/16） with in vivo test. Conclusion BCOP test may be independently used to identify chemicals with potential eye irritation and serious eye damage， suggesting it is significant for in vitro integrated test strategy for predicting eye irritation due to cosmetics.