Objective:To analyze the viral genome characteristics of the first human influenza A(H7N9) infection in Wuhu city in 2014.Methods:Samples from a patient diagnosed as positive for the influenza virus A(H7N9) nucleic acid (detected by real-time RT-PCR) were sent to Chinese National Influenza Center for the isolation of strains and genome-wide sequencing. The sequences were submitted to GenBank and Blast was run. Analysis of the homology, key amino acid site mutations and phylogenetic tree of the viral genome were performed with the use of bioinformatics applications, DNAStar Lasergene 7.1 and MEGA7.0.Results:The strain was named A/Wuhu/1/2014(H7N9). Phylogenetic tree analysis of hemagglutinin(HA) showed that the strain belonged to clade W2-4. The cleavage site of HA was PEIPKGR↓G. Compared with the highly pathogenic vaccine strain A/Guangdong/17SF003/2016(H7N9), it lacks 4 amino acid KRTA insertion sequences. T160A, G186V and Q226L mutations occurred in the HA receptor binding site. Five QISNT amino acids (position 69-73) were deleted in the neuraminidase(NA) stalk region. L89V, M535L and E627K mutations were found in PB2. Site mutations occurred in PB1 I368V, PA V100A, K356R and S409N, NS1 P42S and N205S, NS2 T48A, M1 N30D and T215A. The E119V, R152K, H274Y, and R292K mutations were not found in NA, while the S31N mutation appeared in M2.Conclusions:The A/Wuhu/1/2014(H7N9) strain is derived from poultry and originated in the Yangtze River Delta. The virus has an affinity to human receptors and belongs to the low pathogenic avian influenza A (H7N9) viruses (LPAI H7N9). It is sensitive to NA inhibitors and resistant to M2 ion channel inhibitors. The strain has increased virulence in mammals and enhanced adaptability for humans.

Objective To determine the genotype of dengue virus (DENV) and its molecular characteristics of E gene from 1 case imported from Bangladesh to Wuhu city.Methods The viral RNA was extracted from patient serum sample,the serotype of DENV was determined by Real-time RT-PCR through screening the universal and serotypes 1/2/3/4 of DENV.The completed sequence of DENV E gene was amplified,the phylogenetic tree of E gene was constructed by MEGA 7.0 software,and the nucleotide and amino acid sequences alignments of E gene were analyzed.Results Patient serum was positive for DENV type 2 nucleic acid.The DENV type 2 belonged to the Cosmopolitan genotype by phylogenetic analysis of the E gene,and it had the highest homology with the isolate D2/Malaysia/1408aTw which was imported from Malaysia to Taiwan in 2014,the homologies of nucleotide and amino acid reached 99.60％ and 99.80％,respectively.Totally 6 nucleotides (42:G→A;141:G→A;490:G→A;516:A→G;954:A→G;1344:C→ T) and 1 amino acid (164:V→I) had changed in E gene.E protein possessed attenuated strain characteristic site E126-E and virulent strain characteristic site E383-385-E-P-G.Conclusions The imported patient is infected with DENV type 2 and the virus is originated from Malaysia.