1.Protective effect of combined NGF and ganglioside GM_1 on hypoxic-ischemic brain injury of newborn rats
Wei HU ; Congmin ZHAO ; Shoupin GONG
Journal of Third Military Medical University 2003;0(24):-
Objective To study the therapeutic effect of combined administration of nerve growth factor(NGF)and ganglioside GM_ 1 on the hypoxic-ischemic brain injury(HIBI)rat model and the relevant involving mechanisms.Methods A total of 120 7-day old rats were randomly divided into 5 groups:sham-operation group,HIBI group,NGF group,GM_ 1 group and NGF+GM_ 1 group.HE staining,immunohistochemical staining and TUNEL staining were employed respectively to study the morphological changes,bcl-2 and Bax expressions and apoptosis in the hippocampal neuron after treatment.Results Pathological changes were observed in the hippocampal neuron after HIBI.In NGF+GM_ 1 group,the level of Bcl-2 expression was increased significantly(P
2.Investigation of hsa-miR95-5p,hsa-miR-548am-5p and hsa-miR-1246 in pathogenesis of gestational diabetes mellitus
Wei LI ; Baochun HU ; Zhao GONG
The Journal of Practical Medicine 2016;32(4):566-568,569
Objective To investigate the expression of microRNA (miRNA) in placenta tissue, explore the function of miRNA in pathogenesis of gestational diabetes mellitus (GDM). Methods Placenta tissue from pregnant women with GDM and normal controls were selected from January 2013 to January 2014. Expression of miRNAs were detected by sequencing technique and quantity real time PCR(qRT-PCR). Target genes of miRNA were analyzed by KEGG. Results 52 insulin signaling pathway related miRNAs including 47 up-regulated and 5 down-regulated were differentially expressed in GDM compared with normal control. Expression of placental hsa-miR-548am-5p and hsa-miR-95-5p were significantly increased , but hsa-miR-1246 was significantly lower (P <0.05) in GDM group than normal control; all the results were consistent with sequencing results. Key points of insulin signaling pathway could regulate expression of targets such as PI3K. Conclusion miRNA expression in GDM placenta was abnormal.A variety of exp ressions of miRNAs were involved in the pathogenesis of GDM by regulating insulin signaling pathway.
3.Effects of 3-AB on PARP expression of Hela cells and apoptosis and cell cycle progression of Hela cells after X-rays irradiation
Xiang DU ; Hongguang ZHAO ; Wen WANG ; Wei GUO ; Shouliang GONG
Journal of Jilin University(Medicine Edition) 2006;0(03):-
Objective To study the changes of apoptosis and cell cycle progression of Hela cells after the poly(ADP-ribose) polymerase(PARP) was inhibited by its inhibitor 3-aminobenzamid(3-AB) and the mechanisms of PARP interaction with Hela cells damaged by irradiation.Methods Hela cell line was used.Flow cytometry(FCM) was used to examine the PARP expression of control and 3-AB groups at 0,2,4,8,12 h after administration with 5 mmol?L-1 3-AB.The percentage of apoptotic cells and cell cycle progression of control,irradiation,3-AB plus irradiation groups were measured with FCM at 2,8,12,24 h after exposure to 2 Gy irradiation following administration with 5 mmol?L-1 3-AB.Results The percentage of Hela cells with positive expression of PARP protein decreased after administration with 3-AB and there was significant difference between 3-AB plus irradiation group and control group(P
4.Nosocomial Infection in ICU
Liqun ZHAO ; Junwen YI ; Wei GONG ; Kan XU ; Kanglong YU
Chinese Journal of Nosocomiology 2009;0(24):-
OBJECTIVE To investigate the site of nosocomial infection in ICU,distribution and resistance of bacteria in order to make the intervention strategy.METHODS Antimicrobial susceptibility of pathogenic bacteria isolated from nosocomial infection patients in ICU from Jul 2006 to Jul 2008 was performed by Kirby-Bauer method.RESULTS The main pathogens of nosocomial infection in ICU were Gram-negative organisms(48.09%),Gram-positives(38.93%),and fungi(12.98%).The most common pathogens were Staphylococcus aureus,Acinetobacter baumannii,Pseudomonas aeruginosa,fungi,and S.haemolyticus.All strains of S.aureus and S.haemolyticas were antimicrobial sensitive to vancomycin,teicoplanin and linezolid.All A.baumannii strains were antimicrobial sensitive to cefoperazone/sulbactam.They were resistant to other antimicrobial agents.CONCLUSIONS Gram-negative organisms are the main pathogenic bacteria of nosocomial infection in ICU,but the percentage of Gram-positives and fungi is increasing,S.aureus is the most main pathogenic bacterium of nosocomial infection in ICU.S.haemolyticus is also a main pathogenic bacterium.The pathogenic bacteria of nosocomial infection in ICU are highly resistant to the most antimicrobial agents.
5.Umbilical cord ligation in the fetocide of complicated monochorionic multiple gestations
Yuan WEI ; Lijun GONG ; Guangwu XIONG ; Pengbo YUAN ; Yangyu ZHAO
Chinese Journal of Obstetrics and Gynecology 2013;48(10):750-754
Objective To evaluate the clinical effect and safety of umbilical cord ligation in the fetocide of complicated monochorionic multiple gestations.Methods From January 2009 to December 2012,clinical data of 18 women with complicated monochorionic multiple gestations who experienced intrauterine percutaneous umbilical cord ligation in Peking University Third Hospital were collected.Among the patients,6 were selective intrauterine growth restriction (1 with type Ⅰ,4 with type Ⅱ,1 with type Ⅲ) ; 4 were acrania or hydropic twins; 4 were aeardiac twins,2 were complicated triplet gestation; 1 was twin-twin transfusion syndrome with right ventricular dysplasia and 1 was monochorionic diamniotic (MCDA) with caesarean section history.The procedure was performed under both endoscopic and sonographic guidance.The gestational age at the time of the procedure were 17-27 +6 weeks.The procedure and perinatal outcome were analyzed.Results (1) The procedure was performed successfully in all the 18 cases.The average duration of the procedure was 63 min (24-156 min).The blood loss was 7.6 ml (5-20 ml).The mean gestational age at the time of the procedure was 20 weeks (17-27 +6 weeks).The average birth weight of the neonates was 2441 g(1000-3400 g).(2) There were 20 fetuses survived.Two fetuses had cardiac anomalies and were terminated in the following 2-3 weeks.Intrauterus fetal demise occured in 3 twin reverse arterial perfusion syndrome (TRAP) cases 3-14 weeks after the procedure.1 case delivered as early preterm birth at 28 weeks and the neonate died of respiratory distress syndrome (RDS) and hypoxie-ischemicencephalopathy (HIE).Fourteen neonates were in healthy and normal development by 3-51 months' followup.(3) Fourteen cases delivered at more than 28 weeks (28-38 weeks,averagely 33 + 1 weeks).The gestational weeks were prolonged by 5-21 weeks (averagely 13 +4 weeks).Among them,3 case were early preterm birth (28-33 weeks) and 3 were late preterm birth (34-36 weeks).Conclusion Percutaneous umbilical cord ligation is a reliable technique for the fetocide of complicated monochrionic mutilple gestations,especially for monochronic monoamniotie pregnancies.
6.Determination of the contents of eugenol in Dijin spray by GC-MS
Aiwu WANG ; Qinghua ZHAO ; Wei GONG ; Jie MIAO
International Journal of Traditional Chinese Medicine 2014;36(3):237-239
Objective To determine the eugenol in Dijin spray by GC-MS.Methods The chromato-graphic column was DB-5 fused silica capillary column.Eugenol was determined by program temperature risen method:the initial temperature was 60 ℃ for 1 min,then raised to 200 ℃ at the rate of 10 ℃/min and kept for 5 min,and at last raised to 250 ℃ at 15 ℃/min and kept for 5 min.Electron energy was 70ev.The ions of 164,149,and 77 were selected for eugenol detection.Results The calibration curve of eugenol was linear in the range of0.116~1.16 mg/ml (r=0.999 4) with the average recovery of 97.84% (RSD=0.47%).Conclusion The method was quick,accurate and repeatable.It was suitable for the quality control of Dijin spray.
7.Development of the Structures and Characteristics of Microcapsules
Wei-Yan HAN ; You-Xi ZHAO ; Ping GONG ;
China Biotechnology 2006;0(01):-
Microcapsule technology recently has been applicated and developed in pharmaceutical,chemical,food and so on.The achievments have been obtained on the novel technics of microcapsulation,analytical methods of microcapsule characteristics,and methods of characterization for the appearance and porous structures of microcapsules.The novel developments on the structure and property of microcapsules were reviewed.
8.Quality standard for Gubiling Capsules
Zhu JIN ; Hong WEI ; Bingjun LI ; Quancheng ZHAO ; Xuguo GONG ;
Chinese Traditional Patent Medicine 1992;0(05):-
AIM: To establish the quality standard for Gubiling Capsules (Penis Et Testis Canis, Radix Noto ginseng, Radix Achyranthis Bidentatae, Herba Epimedii, Radix Aucklandiae, etc.). METHODS: Radix Achyranthis Bidentatae, Herba Epimedii, Radix Aucklandiae were identified by TLC, and the ginsenoside Rg 1 content was determined by HPLC. RESULTS: Ginsenoside Rg 1 showed a good linear relationship in the range of 1.2-6.0 ?g ( r = 1.00 0 ) and average recovery was 96.9%. RSD was 0.4%. CONCLUSION: These methods are simple, accurate and specific and can be used for the quality control for Gubiling Capsules.
9.Study on the quality standard of common selfheal fruit-spike granules
Wei GONG ; Xu SUN ; Kun ZHANG ; Zhongliang LIU ; Qinghua ZHAO ; Xingdong LI ; Mei ZHAO ; Xinxin ZHANG
International Journal of Traditional Chinese Medicine 2015;(5):435-438
Objective To establish the quality standard of common selfheal fruit-spike granules. Mehtods Rosmarinicacid in the granules were identified by TLC. The extractives were tested by the method for determination of water-soluble extractives. The contents of rosmarinicacid were determined by HPLC. Results TLC had obvious characteristics with the spots clear and well-separated. The extraction limit was no less than 10.0%. Rosmarinicacid showed a good linear relationship at 0.062 5~2.008 0 mg/ml (r=0.999 8,n=6) with average recovery of 99.50% (RSD=1.21%,n=9). The content limitation of rosmarinicacid was discussed as 10.0 mg per pack according to the experiment data.Conclusion The method is easy-operated and accurate which has a good specificity for the quality control of common selfheal fruit-spike granules.
10.Determination of (R,S)- Epigoitrin in Banlangen Granules by HPLC
Wei GONG ; Zhongliang LIU ; Qinghua ZHAO ; Fenge WEN ; Peng LI ; Kun ZHANG ; Mei ZHAO
Herald of Medicine 2015;(6):791-795
Objective To establish HPLC method for determination of (R,S)-epigoitrin in Banlangen granules and discuss the content limitation. Methods The samples were separated on SHIMADZU VP-ODS (150 mm×4. 6 mm, 5 μm) and the mobile phase consisted of methol-0. 02% phosphoric acid solution (7:93) at a flow rate of 1. 0 mL. min-1 . The detection wavelength was set at 245 nm and the injection volume (in automatic sampler) was 10 μL. The content limitation was assessed according to the transfer rate and statistical data of the results. Results (R,S)-epigoitrin showed a good linear relationship at concentration of 0. 058 7 – 150. 349 5 μg·mL-1(r = 0. 999 7, n = 7). The average recovery rate was 98. 72% , 98. 40% and 98. 60% , respectively; RSD was 1. 84% , 0. 50% and 1. 82% , respectively. The content limitation of (R,S)-epigoitrin was unreasonable according to the transfer rate and the statistical data of the results. Conclusion The method is easy and simple to operate, accurate and stable in results, and highly specific, thus it is applicable for the quality control of Banlangen granules. The content limitation should be determined on the basis of pharmacokinetic and pharmacodynamic data.