1.Diagnostic value of nerve conduction function and F wave in children with lower limbs paralysis.
Li WANG ; Qin ZHANG ; Cheng-gong FENG
Chinese Journal of Pediatrics 2008;46(2):146-148
Adolescent
;
Child
;
Child, Preschool
;
Female
;
Humans
;
Infant
;
Infant, Newborn
;
Male
;
Neural Conduction
;
physiology
;
Paraplegia
;
diagnosis
;
physiopathology
;
Sural Nerve
;
physiopathology
2.Clinical analysis of chemotherapy plus radiotherapy in locally advanced nasopharyngeal carcinoma
Tao WANG ; Gong CHENG ; Yunfeng ZHOU ;
China Oncology 2001;0(03):-
Purpose:To study the influence of chemotherapy on radiotherapy in locally advanced nasopharyngeal carcinoma. Methods:96 patients with histologically proven nasopharyngeal carcinoma in stages Ⅲ,Ⅳ were divided randomly into two groups. Radiotherapy group (48 cases) only received radiotherapy with DT66—76Gy in nasopharynx and DT50—70Gy in the neck. Chemoradiotherapy group (48 cases) received chemotherapy with cisplatin and 5 fluorouracil before the same radiotherapy as the radiotherapy only group. Results:The 1,3,5 year survival rates were 94.2%,72.9%,65.7% in chemoradiotherapy group and 89.1%,56.8 %,46.8% in radiotherapy group respectively, with significant difference between the two groups ( P
3.Roles of AMP-activated protein kinase in cerebral ischemia
Zhenqing GONG ; Yongjiu WANG ; Yan CHENG
International Journal of Cerebrovascular Diseases 2013;(3):216-221
Adenosine monophosphate-activated protein kinase (AMPK) is a serine/threonine protein kinase,which is a energy regulation switch in the cells of the body.In the case of nutritional deficiencies and ischemia,AMPK system is activated as the metabolism and stress signal transduction component to regulate the expression of downstream target proteins.After acute ischemic stroke,AMPK is activated and aggravates neuronal apoptosis,and giving AMPK inhibitor may reduce cerebral ischemic injury.The activation of AMPK after stroke may result in the upregulation of brain-derived neurotrophic factor expression and the activation of endothelial nitric oxide synthase,and they play the protective roles for neuronal regeneration and repair.This article reviews the advances in research on the roles of AMPK in experimental cerebral ischemia.
4.Resveratrol neuroprotection in cerebral ischemia
Yongjiu WANG ; Zhenqing GONG ; Yan CHENG
International Journal of Cerebrovascular Diseases 2012;20(7):542-546
Resveratrol is a polyphenolic compounds in many plants.It has many effects inchuding antioxidation,anti-inflammation,inhibiting apoptosis,and scavenging free radicals.Resveratrol preconditioning has neuroprotective effect in ischemia-reperfusion in rats by activating silent information regulator 2 homolog 1.This effect is similar to brain ischemic preconditioning.In addition,resveratrol can also attenuate the brain infraction volume of cerebral ischemia/reperfusion in mice and improve neurological function.This article reviews the neuroprotective effect of resveratrol and its mechanisms in cerebral ischemia.
5.Resveratrol protects oxygen-glucose deprivation rat cortical neurons by regulating SIRT1/AMPK signaling pathways
Yongjiu WANG ; Zhenqing GONG ; Zhihui WANG ; Yan CHENG
International Journal of Cerebrovascular Diseases 2015;(3):193-197
Objective To investigate the effects of resveratrol on the first and double oxygen-glucose deprivation (OGD) primary cortical neuron silent information regulator 1 (SIRT1), AMP-activated protein kinase (AMPK) activity and ATP content, and its possible neuroprotective mechanism. Methods Cortical neurons were taken from the embryos of 18-day Wistar rats. An in vitro repeated ischemia model was induced by the double OGD after the success of primary culture. Trypan blue stalning was used to detect the cel survival rate. Western blot was used to detect the SIRT1 and phospho-AMPK expression. Deacetylase fluorescence assay was used to detect the SIRT1 activity. Bioluminescence assay was used to detect the ATP content. Results Compared with the control group, resveratrol (0. 5 μmol/L) preconditioning significantly increased the survival rates after the single and double OGD (al P < 0. 001), ATP content (al P = 0. 004), SIRT1 activity (single: P = 0. 001; double: P = 0. 002), and the expression levels of SIRT1 (single: P = 0. 029; double: P = 0. 023) and phospho-AMPK (al P = 0. 001). Conclusions Resveratrol has the neuroprotective effect for the first and double OGD cortical neurons. Its mechanism may be associated with upregulating the SIRT1/AMPK signaling pathways and decreasing the energy requirements.
6.Value of Oblique Axial MPR Imaging of MSCT in Diagnosing the Fracture of Geniculate Fossa of Facial Nerve Canal and Its Nearby
Yuhua LI ; Ruozhen GONG ; Tao WANG ; Cheng LIU ; Dailun HOU
Journal of Practical Radiology 1996;0(04):-
Objective To evaluate the diagnostic value of oblique axial multiplanar reformation(MPR)imaging in detecting the fracture of geniculate fossa of facial nerve canal and its nearby.Methods 33 cases of facial nerve canal or nearby fracture confirmed by surgery were collected.CT findings(including conventional axial,coronal and oblique axial MPR images)were retrospectively analysed in comparison with that of operation.The diagnostic corresponding rate of two diagnostic modes(the combination of routine axial and coronal imagings,the combination of routine axial,coronal and oblique axial imagings)were compared.Results The fractures of geniculate fossa or the proximate of tympanic segment were seen in 22 cases on pre-operation CT images,including 8 cases on routine axial images,6 cases on routine coronal images and 20 cases on oblique axial MPR images.Local enlargement of geniculate fossa or the proximate of tympanic segment were seen in 26 cases on oblique axial MPR images.There were significant differences between these two diagnostic modes.Conclusion Oblique axial MPR images in combined with routine axial and coronal images can improve the diagnostic corresponding rate of the fracture of geniculate fossa and its nearby.
7.Clinical analysis of 52 patients with ischemic colitis
Xianghu ZHU ; Feng WANG ; Xuchen GONG ; Min CHENG
International Journal of Surgery 2011;38(5):312-314
Objective To investigate clinical and endoscopic features of isehemic colitis(IC)to further enhance the awareness of the disease.Methods A retrospective analysis from June 1999 to August 2009,52of IC patients with clinical presentation and endoscopic findings were recorded and restrospectively analysed.Results Thirty-eight patients(73.08%)had vascular disease,with clinical manifestations of acute abdominal pain,diarrhea,blood in the stool and fever.Sigmoid colon lesions occurred in 35 cases(67.31%),lesions 4-30 cm.Condusions Abdominal pain is a clinical feature of acute ischemic colonic disease,endescoPy is an effective way to diagnose this disease,raise awareness of the disease,especially for those complicated with underlying diseases,and early colonoscopy are important for a definite diagnosis of IC.
9.Study on informal communication in research management under community of practice mode
Xueyan GONG ; Fan YANG ; Cheng WANG ; Xing PI
Chinese Journal of Medical Science Research Management 2017;30(1):6-8
Research team shows virtual and distributed characteristics in the big science era.It is common and frequent that researcher often communicates with each other informally for knowledge sharing regularly or non-regularly.Thus raised the attention paid by research administrators to such informal communication in the research team.From the aspect of community practice,we tried to propose helpful suggestions and considerations to improve the effectiveness of informal communication from following four perspectives:focusing on the informal social network in research team,create conditions for informal communication,train communication coordinators and measure the values of informal communication scientifically.
10.Cholesteryl ester transfer protein level in male patients with different degrees of coronary artery lesion and its clinical significance
Jinghua CHENG ; Jianbin GONG ; Chunni ZHANG ; Junjun WANG ; Shisen JIANG
Chinese Journal of Clinical Laboratory Science 2006;0(01):-
Objectives To explore cholesteryl ester transfer protein(CETP)level in male patients with different degrees of coronary artery lesion and its clinical significance.Methods ELISA was used to measure CETP of 42 male patients with coronary heart disease(CHD)and 49 healthy male controls.The patients of CHD groups were subdivided into mono-vessel,ambi-vessel,multi-vessel lesion groups;localized,diffuse lesion groups;and mild,severe stenosis groups according to coronary angiography.Results The CETP level of patients with CHD(1.37?1.07 mg/L)was significantly higher than that of healthy control(0.99?0.53 mg/L)(P