Objective To compare the probability of anastomotic leak in the Chinese patients with carcinoma of esophagus using anastomat anastomose and manual anastomose in order to provide some refer -ence for patients with carcinoma of esophagus .Methods By meta-analysis, we made a comprehensive a-nalysis of 12 literatures published in China on anastomat anastomose and manual anastomose in preventing anastomotic leak in patients with carcinoma of esophagus .Results The probability of anastomotic leak in patients with carcinoma of esophagus was obvious heighten in using anastomat anastomose compared to that in using manual anastomose,with the combined odds ratio(OR)value being 0.53(95%CI:0.40-0.71) >0,( P <0.01 ).Conclusion To preventing anastomotic leak in patients with carcinoma of esophagus , anastomat anastomose is more predominant than manual anastomose .

Because of the lack of professional knowledge guidance,in addition to the blind fear and eating disorders,leading to the incidence of hypertension in rural community of China was Showing an up-ward trend year by year.Hypertension was a chronic disease,was the potential risk factor for cardiovascular and cerebrovascular disease,severe heart,the function of brain,kidney and other important organ were fail-ure,to made people's life health and quality of life received threats.With the development of economy and society and the acceleration of the process of population aging,hypertension in the rural elderly population had became a common disease.,as a representative of non communicable chronic diseases,hypertension has seriously affected the normal development.Specification and strengthening the management of hyperten-sion in rural areas has become a hot topic,At present,mainly to the community as the main front for the prevention and treatment of hypertension.The traditional model of community hypertension management has gradually been eliminated.In recent years,China has carried out various aspects of community hypertension management model,In this study,we analyzed the community management of hypertension in rural areas in recent years,and made the following survey.

Various properties of the raw drug Horsc Bozoar were studied by microscopic observation, X-ray phase analysis, thermal analysis, elementary analysis and solubility test .Resul.ts showed that Horse Bezoar mainly contains magnesium ammonium phosphate as well assome magnesium -phosphorus stones.

Objective: To establish an experimental unilateral internal carotid artery stenosis model in New Zealand albino rabbits that can be detected in vivo. Methods: A 2 F Foley's tube was inserted into unilateral carot id artery for performing intimal injury in 5 rabbits, and causing intimal hyperplasia and angiostenosis. The hemodynamic changes of unilateral carotid artery were detected by transcranial Doppler (TCD); the reduction of cerebral blood flow (CBF) after stenosis was calculated by mathematical formulas. The degree of stenosis was confirmed by digital subtraction angiography (DSA), and the arterial intimal hyperplasia and stenosis were confirmed by pathological examination. Results: Two rabbits survived after the procedure. TCD, DSA and pathological examination showed that the model of experimental unilateral internal carotid artery stenosis in rabbits was established successfully. The reduction of CBF after unilateral internal carotid artery stenosis was calculated in the 2 rabbits, they were 17% and 45%, respectively. Conclusion: The rabbit model of unilateral internal carot id artery stenosis has been established successfully, and the severity of stenosis can be detected and evaluated in vivo.

Acute pancreatitis (AP) is activated trypsin-induced pancreatic and its peripheral tissue inflammation caused by their own digestion.The activation in advance of trypsinogen and the inflammation cascade in pancreatic acinar cells are thought to be a key mechanism of the onset and development of AP.Autophagy pathway acting as Ⅱ type of programmed cell death occurs in the early pathological course of AP,blockade of which contributes to aggravating necrosis of acinar cells in AP.This article mainly discussed the recent advances in the understanding of autophagy researches and its function in the mechanism of AP.

Objective To discuss the value of enlargement of geniculate fossa of facial nerve canal in the diagnosis of facial nerve canal fracture.Methods Thirty patients with facial nerve canal fracture underwent axial and coronal CT scan.The correlation between the fracture and the enlargement of geniculate fossa of facial nerve canal was analyzed.The ability of showing the fracture and enlargement of geniculate fossa of facial nerve canal in axial and coronal imaging were compared.Results Fracture of geniculate fossa of facial nerve canal was found in the operation in 30 patients,while the fracture was detected in CT in 18 patients.Enlargement of geniculate ganglion of facial nerve was detected in 30 patients in the operation,while the enlargement of fossa was found in CT in 28 cases.Enlargement and fracture of geniculate fossa of facial nerve canal were both detected in CT images in 18 patients.Only the enlargement of geniculate fossa of facial nerve canal was shown in 12 patients in CT.Conclusion Enlargement of geniculate fossa of facial nerve canal was a useful finding in the diagnosis of fracture of geniculate fossa in patients with facial paralysis,even no fracture line was shown on CT images.

Acoustic Stimulation ; Animals ; Auditory Perception ; physiology ; Behavior, Animal ; physiology ; Noise ; adverse effects ; Rabbits ; Sound ; Swine

Objective　To construct a recombinant plasmid pcDNA3-sHLA-G1 expressing soluble HLA-G1. Methods　 Total cell RNA was extracted from the cell line Jeg-3 and the cDNA was amplified by RT-PCR； The cDNA fragment was inserted into the eukaryotic expressing vector pcDNA3 and the recombinant plasmid was identified by restriction endonucleases digestion and sequencing. Results　 After restriction endonucleases treatment and sequencing, it was confirmed that the pcDNA3-sHLA-G1 had been constructed successfully. Conclusion　 In this study, the recombinant plasmid pcDNA3-sHLA-G1 had been constructed successfully.

ObjectiveTo investigate the effects of different ways of fluid resuscitation on uncontrolled traumatic hemorrhagic shock. MethodsThe retrospective analysis of data from 220 casualties with uncontrolled hemorrhagic shock admitted from September 2006 to October 2010 was carried out.There were two ways of fluid resuscitation used to treat casualties with hemorrhagic shock,conventional fluid resuscitation (group A) and limited fluid resuscitation (group B).The systolic pressure was maintained at 90 ～ 135 mm Hg in the group A and at 70 ～ 90 mm Hg in the group B.The data of body temperature,central venous pressure,quantity of blood transfused,urine output,renal function,hemoglobin,blood osmotic pressure (Osm) ,MODS and DIC rates,treatment costs and mortality of two groups were statistically analyzed and compared. Results After analyses of above mentioned variables except renal function and comparison made between two groups,the method of limited fluid resuscitation was much superior over conventional fluid resuscitation (P ＜ 0.05 ).There was no noticeable difference in rate of compromised renal function between two groups ( P ＞ 0.05 ). Conclusions In the case of uncontrolled exsanguination,the method of limited fluid resuscitation can maintain blood perfusion of vital organs,reduce the blood loss and decrease the incidence of MODS and mortality.

BACKGROUND: Stem cells, cells with special function in animals and humans, exist in various tissues. Most of stem cells differentiate into special tissue organs and some of them remain in the status of stem cells for tissue repair. Mesenchymal stem cells were transplanted to burn wounds in some researches for inducing the proliferation and activation of skin stem cells so as to cure burn.OBJECTIVE: To observe the effect of stem cell culture medium cultured in vitro instilled locally into the severely traumatic skin in guinea pigs on healing time and healing degree of the wound.DESIGN: Random grouping and blank control trial.SETTING: Department of Toxicology, School of Public Health, Jilin University.MATERIALS: Totally 14 adult healthy guinea pigs of either gender weighing 300 to 350 g were recruited.METHODS: The experiment was conducted in the Laboratory of Toxicology Department, School of Public Health, Jilin University, from March to September 2003. Ten guinea pigs were put to death by bloodletting on the neck. The bone marrow was extracted and cultured in unicellular supematant fluid for use. The 14 guinea pigs were made into models of bilateral severe skin trauma.Ten of the guinea pigs were chosen randomly, stem cell culture was instilled into one side of the animals (stem cell group), while the culture medium was instilled into the other side of the animals (culture medium group). The remaining 4 guinea pigs that received no treatment were blank control group.Three days later, transparent lucite was put on the wound every other day for drawing the shape and observing the wound. After the shape was copied onto the transparent lucite, the wound area was worked out on the rectangular coordinate paper and the speed of wound healing was calculated.MAIN OUTCOME MEASURES: Gross observation was performed on the healing status of the wound and average healing time and speed of the guinea pigs in each group.wound healing status of the guinea pigs in each group: At day 3, the wound in stem cell group was dry without obvious exudation. There was a layer of membrane at the bottom of the wound and it stuck to the pledget closely so that the dressing could not easily be removed. The wound status in the culture medium was practically the same as that of the stem cells, but no membrane-ike material was formed and the dressing was easily removed. Inflammation appeared obviously in blank control group.nificantly less in stem cell group than in culture medium group and blank control group [(12.45±2.18) days vs (26.29±1.38) days and ＞ 30It was obviously faster in stem cell group than in culture medium group and blank control group [(40.42±2.14) mm2 per day vs (15.53±5.22) mm2 per day and(10.27±4.57) mm2 per day,P ＜ 0.05].CONCLUSION: The stem cells of homologous animals were instilled on the skin surface to repair injury wound. Stem cells can grow on the wound and transform into skin cells, which promotes the recovery of wounded skin. Therefore, it is feasible to treat severe skin defect with stem cells.