1.Effect of angiotensin II on follicular atresia in mouse.
Yong CHENG ; Li-Hong JIAO ; Rui-Hua LIU ; Qing-Bin WANG ; Hong WANG ; Guo-Liang XIA
Acta Physiologica Sinica 2002;54(1):75-78
The effect of angiotensin II (Ang II) on the follicular development was studied by using an animal model of follicular atresia induced by pregnant mare s serum gonadotropin (PMSG). The results showed that: (1) a large number of atretic follicles were found in the ovary of 24-day-old mouse after 6-day treatment of PMSG. Deoxyribonucleic acid (DNA) extracted from granulosa cells clearly showed a ladder band under agarose gel electrophoresis analysis. (2) the contents of Ang II in the ovary extremely increased with the development of follicular atresia. (3) Ang II significantly antagonized the stimulating effect of the follicle-stimulating hormone (FSH) on estradiol (E(2)) generation of granulosa cells. It is suggested that Ang II may be involved in the regulation of follicular atresia in mouse.
Angiotensin II
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pharmacology
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physiology
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Animals
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Cells, Cultured
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Estradiol
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biosynthesis
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Female
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Follicle Stimulating Hormone
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pharmacology
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Follicular Atresia
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physiology
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Gonadotropins, Equine
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pharmacology
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Granulosa Cells
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drug effects
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metabolism
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Mice
2.Effects of echinomycin on endothelin-2 expression and ovulation in immature rats primed with gonadotropins.
Zhengchao WANG ; Zhenghong ZHANG ; Yanqing WU ; Liyun CHEN ; Qianping LUO ; Jisen ZHANG ; Jiajie CHEN ; Zimiao LUO ; Xiaohong HUANG ; Yong CHENG
Experimental & Molecular Medicine 2012;44(10):615-621
Echinomycin is a small-molecule inhibitor of hypoxia-inducible factor-1 DNA-binding activity, which plays a crucial role in ovarian ovulation in mammalians. The present study was designed to test the hypothesis that hypoxia-inducible factor (HIF)-1alpha-mediated endothelin (ET)-2 expressions contributed to ovarian ovulation in response to human chorionic gonadotropin (hCG) during gonadotropin-induced superuvulation. By real-time RT-PCR analysis, ET-2 mRNA level was found to significantly decrease in the ovaries after echinomycin treatment, while HIF-1alpha mRNA and protein expression was not obviously changed. Further analysis also showed that these changes of ET-2 mRNA were consistent with HIF-1 activity in the ovaires, which is similar with HIF-1alpha and ET-2 expression in the granulosa cells with gonadotropin and echinomycin treatments. The results of HIF-1alpha and ET-2 expression in the granulosa cells transfected with cis-element oligodeoxynucleotide (dsODN) under gonadotropin treatment further indicated HIF-1alpha directly mediated the transcriptional activation of ET-2 during gonadotropin-induced superuvulation. Taken together, these results demonstrated that HIF-1alpha-mediated ET-2 transcriptional activation is one of the important mechanisms regulating gonadotropin-induced mammalian ovulatory precess in vivo.
Animals
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Cells, Cultured
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Chorionic Gonadotropin/*pharmacology
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Echinomycin/*pharmacology
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Endothelin-2/genetics/*metabolism
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Female
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Gonadotropins, Equine/*pharmacology
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Granulosa Cells/drug effects/metabolism
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Humans
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Hypoxia-Inducible Factor 1, alpha Subunit/*antagonists & inhibitors/genetics/metabolism/physiology
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Oligonucleotides/genetics
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Ovary/cytology/drug effects/physiology
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Rats
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Rats, Sprague-Dawley
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Superovulation/*drug effects
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Transcriptional Activation