No abstract available.
Osteoblasts*

No abstract available.
Teratoma*

No abstract available.
Complement System Proteins* ; Otitis Media with Effusion* ; Otitis Media* ; Otitis*

No abstract available.
Gynecomastia* ; Male

The morphology of the lingual papillae in a female Bengal tiger (Panthera tigris tigris) was examined by scanning electron microscopy (SEM). The tongue was 22.3 cm in length and 7.1 cm in width. Numerous filiform papillae were distributed over the entire dorsal surface of the tongue. SEM examination of the tongue revealed two types of mechanical papillae, i.e. filiform and conical papilla, and two types of gustatory papillae, i.e. fungiform and vallate papilla, on the dorsal surface of the tongue. Each filiform papilla consisted of one primary papilla and several secondary papillae. The filiform papillae on the anterior part of the tongue were divided into one primary and 6~14 secondary papillae. Unlike other mammalians, however, secondary papillae in the mid-part of the tongue showed pineal-like papillae. In the posterior part of the tongue, secondary papillae were rare or absent. Fungiform papillae were surrounded by filiform papillae and densely distributed on the lingual surface. There were two vallate papillae on the borderline between the lingual body and root of the tongue. A vallate papilla contained two secondary papillae inside the grooves. Conical papillae were located in the area of the vallate papillae and covered the posterior part of the tongue root. No foliate papillae were seen on both margins of the posterior part of the tongue. Our results indicate that the structure on the lingual papillae of the Bengal tiger is somewhat different from that of other mammals.
Female ; Humans ; Mammals ; Microscopy, Electron, Scanning ; Tigers* ; Tongue

PURPOSE: To evaluate the results and complications of the retrograde intramedullary nailing for the treatment of distal femur fracture. MATERIALS AND METHODS: Thirty three patients who received retrograde IM nailing for fractures of the distal femur between October 1998 to December 2003. Average age was 53.8+/-17 (17~86) years. The average follow up period was 19.4 (12~36) months. Clinical information included age, sex distribution, associated fracture and fracture was classified by AO classification. Functional result was evaluated by Schatzker's criteria. RESULTS: The most common cause of injury was traffic accident (60%). The type of fracture were 6 A1 cases, 5 A2 cases, 11 A3 cases, 5 C2 cases, 6 C3 cases by AO classification. Among the 33 cases, 15 cases were excellent, 9 good, 6 fair and 1 failure according to Schatzker's criteria. Average union time was 9.7+/-3.5 months. CONCLUSION: Treatment of distal femur fracture with retrograde intramedullary nailing was useful due to its minimal invasiveness and early range of motion, more rigid fixation.
Accidents, Traffic ; Classification ; Femur* ; Follow-Up Studies ; Fracture Fixation, Intramedullary* ; Humans ; Range of Motion, Articular ; Sex Distribution

Numerous psychotropic and addictive substances possess structural features similar to those of β-phenethylamine (β-PEA). In this study, we selected 29 β-PEA derivatives and determined their structure–activity relationship (SAR) to their ability to inhibit dopamine (DA) reuptake; conducted docking simulation for two selected compounds; and identified their potential functionals. The compounds were subdivided into arylethylamines, 2-(alkyl amino)-1-arylalkan-1-one derivatives and alkyl 2-phenyl-2-(piperidin-2-yl)acetate derivatives. An aromatic group, alkyl group, and alkylamine derivative were attached to the arylethylamine and 2-(alkyl amino)-1-arylalkan-1-one derivatives. The inhibitory effect of the compounds on dopamine reuptake increased in the order of the compounds substituted with phenyl, thiophenyl, and substituted phenyl groups in the aromatic position; compounds with longer alkyl groups and smaller ring-sized compounds at the alkylamine position showed stronger inhibitory activities. Docking simulation conducted for two compounds, 9 and 28, showed that the (S)-form of compound 9 was more stable than the (R)-form, with a good fit into the binding site covered by helices 1, 3, and 6 of human dopamine transporter (hDAT). In contrast, the (R, S)-configuration of compound 28 was more stable than that of other isomers and was firmly placed in the binding pocket of DAT bound to DA. DAinduced endocytosis of dopamine D2 receptors was inhibited when they were co-expressed with DAT, which lowered extracellular DA levels, and uninhibited when they were pretreated with compound 9 or 28. In summary, this study revealed critical structural features responsible for the inhibition of DA reuptake and the functional role of DA reuptake inhibitors in regulating D2 receptor function.

The purpose of this study was to identify the composition and organization of lingual tissues underlying the histo-structural change of developing tongue in Korean native goats by light microscopy (LM). Tongues of the fetuses on days 60, 90, 120 and neonate were examined for the morphological development. In the 60-day-old fetuses, the tongue tissues were differentiated into epithelium, lamina propria and muscle layer. Primordia of filiform, conical, lentiform, fungiform and vallate papillae appeared and rudiments of taste bud were observed in the epithelia of the primordia of the gustatory papillae. The dorsal surface of the lingual epithelia showed a weak PAS positive reaction. Collagenous fibers and small blood vessels were shown in the connective tissues. In the 90-day-old fetuses, Von Ebner's glands were moderately PAS positive while the muscle fibers and connective tissue were strongly positive for PAS. The collagenous fibers increased and came to have a more complex arrangement in the tongue. The muscle fibers were spread out at various directions and developed in striated muscle bundles. In the 120-day-old fetuses, taste buds were observed in the epithelia of the gustatory papillae, and several well-developed tissues visible such as blood vessels, collagenous fibers, muscle fiber bundles and Von Ebner's glands. In the neonates, many taste buds were found in a transverse section of the vallate papilla. The muscle layers, Von Ebner's glands, collagenous fibers and blood vessels were more developed than those of the 120-day old fetuses. These findings indicate that goat tongues have a variety of different shapes during prenatal development.
Blood Vessels ; Collagen ; Connective Tissue ; Epithelium ; Fetus ; Goats* ; Humans ; Infant, Newborn ; Microscopy ; Morphogenesis ; Mucous Membrane ; Muscle, Striated ; Muscles ; Taste Buds ; Tongue* ; von Ebner Glands

Cytokeratin (CK) comprises the intermediate filament cytoskeleton of epithelial cells. Patterns of CK expression can be regarded as a specific marker for epithelial differentiation status. The aim of this study was to identify CK expression on tongues of Korean native goats ranging from 60-day-old fetuses to newborns during prenatal development using immunohistochemistry. The tongues of fetuses were removed from 2- to 4-year-old female Korean native goats by caesarean section performed under general anesthesia. Immunohistochemistry was performed to assess CK expression patterns on developing goat tongues using serial paraffin-embedded sections. Light zones signifying CK immunoreactivity in dorsal lingual epithelia were weakly positive in 60-day-old fetuses. In 90-day-old fetuses, deep areas in dorsal lingual epithelia were strongly positive for CK expression and superficial areas were moderately positive. In 120-day-old fetuses, light zones of lingual epithelia in the vallate papilla were strongly positive for CK expression, whereas ducts of von Ebner's glands were moderately positive. In neonates, taste buds were positive for CK expression, whereas non-taste epithelial cells and von Ebner's glands were negative. These findings indicate that goat tongues have different patterns of CK expression during development and provide a morphological basis for studies on the biological mechanism of epithelial differentiation.
Anesthesia, General ; Cesarean Section ; Child, Preschool ; Cytoskeleton ; Epithelial Cells ; Epithelium ; Female ; Fetus ; Goats* ; Humans ; Immunohistochemistry ; Infant, Newborn ; Intermediate Filaments ; Keratins* ; Pregnancy ; Taste Buds ; Tongue* ; von Ebner Glands

OBJECTIVE: To investigate the effects of leptin on in vitro development of mouse embryos. METHODS: Female C57BL mice, aged 8 to 15 weeks, were superovulated with IP injection of 5 IU of PMSG followed by 5 IU hCG at 48 hours later. And then the mice were mated with male mice. The next morning, one-cell embryos were collected and cultured in media added with various concentrations (0, 5, 50, 500 ng/mL) of leptin for 4 days. In addition, to determine whether the sensitivity to leptin varied at different stages of development, embryos at 2- or 4-cell stage were treated with the same concentrations of leptin and cultured up to blastocyst stage. The total cell number of blastocyst was assessed and the expression of leptin receptor was examined in all stages of development by immnuofluorescence. RESULTS: The rate of blastocyst formation from one-cell embryos significantly increased at culture media that leptin was added at 50 ng/mL concentration, whereas decreased at 500 ng/mL concentration compared to the control (P<0.05). The development rate of embryos, from 2-cell stage, was similar to the rate from 1-cell stage. However, the addition of leptin to culture media in 4-cell embryos had no significant effects on embryo development compared to the control. In addition, the dose-dependent stimulatory or inhibitory effect of leptin on embryo development was weakened at 2-cell and 4-cell embryo stages compared one-cell embryo stage. The total cell number of blastocyst also significantly increased at 50 ng/mL of leptin, but decreased at 500 ng/mL. Leptin receptor was expressed in all stages from one-cell embryos to blastocyst. The intensity of Ob-Rb immnuostaining was mainly stronger in one- or two-cell embryos, decreased with advancing development stages, and increased again in blastocyst. CONCLUSION: This study shows that addition of leptin to embryo culture media affects embryo development in a dose-dependent and developmental stage-dependent manner. The effects of leptin seems to be associated with the expression pattern of leptin receptor at different stages of development.
Aged ; Animals ; Blastocyst ; Cell Count ; Culture Media ; Embryonic Development ; Embryonic Structures ; Female ; Fluorescent Antibody Technique ; Humans ; Leptin ; Male ; Mice ; Mice, Inbred C57BL ; Pregnancy ; Receptors, Leptin