1.Methanolic extract of Momordica cymbalaria enhances glucose uptake in L6 myotubes in vitro by up-regulating PPAR-γ and GLUT-4.
Puttanarasaiah Mahesh KUMAR ; Marikunte V VENKATARANGANNA ; Kirangadur MANJUNATH ; Gollapalle L VISWANATHA ; Godavarthi ASHOK
Chinese Journal of Natural Medicines (English Ed.) 2014;12(12):895-900
The present study was undertaken to evaluate the influence of the methanolic fruit extract of Momordica cymbalaria (MFMC) on PPARγ (Peroxisome Proliferator Activated Receptor gamma) and GLUT-4 (Glucose transporter-4) with respect to glucose transport. Various concentrations of MFMC ranging from 62.5 to 500 μg·mL(-1) were evaluated for glucose uptake activity in vitro using L6 myotubes, rosiglitazone was used as a reference standard. The MFMC showed significant and dose-dependent increase in glucose uptake at the tested concentrations, further, the glucose uptake activity of MFMC (500 μg·mL(-1)) was comparable with rosigilitazone. Furthermore, MFMC has shown up-regulation of GLUT-4 and PPARγ gene expressions in L6 myotubes. In addition, the MFMC when incubated along with cycloheximide (CHX), which is a protein synthesis inhibitor, has shown complete blockade of glucose uptake. This indicates that new protein synthesis is required for increased GLUT-4 translocation. In conclusion, these findings suggest that MFMC is enhancing the glucose uptake significantly and dose dependently through the enhanced expression of PPARγ and GLUT-4 in vitro.
Biological Transport
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Dose-Response Relationship, Drug
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Fruit
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Gene Expression
;
drug effects
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Glucose
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metabolism
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Glucose Transporter Type 4
;
metabolism
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Hypoglycemic Agents
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pharmacology
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In Vitro Techniques
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Insulin
;
metabolism
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Momordica
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Muscle Fibers, Skeletal
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drug effects
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PPAR gamma
;
metabolism
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Plant Extracts
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pharmacology
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Protein Biosynthesis
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Protein Synthesis Inhibitors
;
pharmacology
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Rosiglitazone
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Thiazolidinediones
;
pharmacology
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Up-Regulation