Japanese encephalitis virus (JEV) causes a mosquitoborne viral zoonosis that is becoming increasingly important to public health in east and south Asia. Although JEV is primarily associated with reproductive failure in swine, JEV infection can cause fever and headache in humans and is associated with aseptic meningitis and encephalitis. The exact mode of transmission, including host range and possible source of viral amplification within livestock, is still not completely clear. This study consisted of a serological survey of JEV infection in goats. A total of 804 goat serum samples were collected from 144 farms in Korea between May 2005 and May 2006. The incidence of positive cases was 12.1% (97 out of 804 goats). The seroprevalence of JEV infection in the 144 farms screened was 31.3% (45/144), indicating that JEV infection is frequent in goat farms in Korea. In addition, three districts of Korea (mainly in the southern region) had a higher seroprevalence of JEV compared to other areas. The results suggest that goats could be monitored epidemiologically as a sentinel animal for JEV transmission in Korea.
Age Factors ; Animals ; Antibodies, Viral/blood ; Encephalitis Virus, Japanese/*isolation & purification ; Encephalitis, Japanese/epidemiology/*veterinary/virology ; Goat Diseases/*epidemiology/*virology ; Goats ; Hemagglutination Inhibition Tests/veterinary ; Korea/epidemiology ; Seroepidemiologic Studies

Five cases of orf virus infection in Korean black goats were diagnosed in our laboratory between 2010 and 2011. One orf virus (ORF/2011) was isolated from an ovine testis cell line (OA3.Ts) for use as a vaccine candidate. Sequences of the major envelope protein and orf virus interferon resistance genes were determined and compared with published reference sequences. Phylogenetic analyses revealed that orf viruses from Korean black goats were most closely related to an isolate (ORF/09/Korea) from dairy goats in Korea. This result indicates that the orf viruses might have been introduced from dairy goats into the Korean black goat population.
Animals ; Ecthyma, Contagious/epidemiology/*virology ; Goat Diseases/*epidemiology/virology ; Goats ; Molecular Sequence Data ; Orf virus/*genetics/isolation & purification/metabolism ; Phylogeny ; Polymerase Chain Reaction/veterinary ; Republic of Korea/epidemiology ; Sequence Analysis, DNA/veterinary ; Sequence Homology

Peste des petits ruminants (PPR) diagnosis from suspected samples from sheep and goats was carried out. Buffy coat, tissues, and oculo-nasal swabs were analyzed using nucleoprotein (NP3/NP4) and fusion protein (F1/F2) gene primers, respectively. Analysis of the sample types and primer set revealed that buffy coat are the best type of samples for PPR diagnosis and the use of two set of primers will increase the number of positives.
Animals ; DNA Primers/analysis ; Eye/virology ; Goat Diseases/blood/*diagnosis/epidemiology/virology ; Goats ; Hair/virology ; Nose/virology ; Nucleoproteins/analysis ; Peste-des-Petits-Ruminants/blood/*diagnosis/epidemiology/virology ; Peste-des-petits-ruminants virus/genetics/*isolation & purification ; Pigmentation ; RNA, Viral/genetics/*isolation & purification ; Reverse Transcriptase Polymerase Chain Reaction/*methods/standards/veterinary ; Sheep ; Sheep Diseases/blood/*diagnosis/epidemiology/virology ; Uganda/epidemiology