1.Toxicokinetics of emodin-8-O-β-D-glucoside in rats in vivo.
Qi WANG ; Jian-Bo YANG ; Ying WANG ; Yan-Yi LI ; Hai-Ruo WEN ; Yu-Jie ZHANG ; Shuang-Cheng MA
China Journal of Chinese Materia Medica 2022;47(15):4214-4220
This study aims to establish an ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS) method for the determination of emodin-8-O-β-D-glucoside(EG) and its metabolites in plasma, and to investigate the toxicokinetics(TK) behavior of them in rats. To be specific, the TK of EG and its metabolites from the first to the last administration in the repeated dose toxicity study was determined, and the kinetic parameters were calculated. The exposure of EG prototype and metabolites in rat plasma after oral administration of different doses of EG was evaluated. The result showed that the prototype of EG and its metabolites aloe-emodin-8-O-β-D-glucoside, emodin, aloe-emodin, and hydroxyemodin could be detected in rats after oral administration of high-, medium-, and low-dose EG. The area under the curve(AUC) of the prototype and metabolites after the first and last administration was in positive correlation with the dose. The time to the maximum concentration(T_(max)) of EG and metabolites in the three administration groups was <6 h, and the longest in vivo residence time was 12 h. The T_(max) and in vivo residence time of EG were prolonged with the increase in the dose. The metabolites emodin, aloe-emodin, and hydroxyemodin all had two peaks. Both hydroxyemodin and aloe-emodin exhibited increased plasma exposure, slow metabolism, and accumulation in vivo. In addition, aloe-emodin-8-O-β-D-glucoside and emodin disappeared with the increase in dose, suggesting the change of the metabolic pathway of EG in vivo in the case of high-dose administration. The mechanism of high-dose EG in vivo needs to be further explored. This study preliminarily elucidates the TK behavior of EG in rats, which is expected to support clinical drug use.
Animals
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Anthraquinones
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Chromatography, High Pressure Liquid/methods*
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Emodin/toxicity*
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Glucosides/toxicity*
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Mass Spectrometry
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Rats
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Toxicokinetics
2.Arctiin antagonizes triptolide-induced renal toxicity in rats via anti-inflammatory pathway.
Yuyan ZHOU ; Xiaoya LU ; Li XIA ; Weiqiang YAO ; Guozheng QIN ; Guodong WANG
Journal of Southern Medical University 2020;40(10):1399-1405
OBJECTIVE:
To investigate the protective effect of arctiin with anti-inflammatory bioactivity against triptolide-induced nephrotoxicity in rats and explore the underlying mechanism.
METHODS:
Forty SD rats were divided into 4 groups for gastric lavage of normal saline, arctiin (500 mg/kg), triptolide (500 μg/kg), or both arctiin (500 mg/kg) and triptolide (500 μg/kg). Blood samples were collected for analysis of biochemical renal parameters, and the renal tissues were harvested for determining the kidney index and for pathological evaluation with HE staining. In the
RESULTS:
In SD rats, arctiin significantly antagonized triptolide-induced elevation of BUN, Scr and kidney index (
CONCLUSIONS
Arctiin can protect the kidney from triptolide-induced damages in rats possibly through the anti-inflammatory pathway.
Animals
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Anti-Inflammatory Agents
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Diterpenes/toxicity*
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Epoxy Compounds/toxicity*
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Furans
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Glucosides
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Kidney/drug effects*
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Phenanthrenes/toxicity*
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Rats
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Rats, Sprague-Dawley
3.Literature research of the hepatotoxicity of glucoside tripterygium total and its synergism and toxicity reducing effects.
Hong-Gang LI ; Wei JI ; Jian-Ming SU
Chinese Journal of Integrated Traditional and Western Medicine 2012;32(3):415-418
Correlated literatures of the liver injury induced by glucoside tripterygium total (GTT) and of its synergism and toxicity reducing effects were retrieved to study the correlation between the dosage, the time point of GTT and the liver injury. The monomer or active ingredients of animals and plants with the hepatoprotective effect were included. The mechanisms of action between GTT and these drugs were analyzed. The mechanism for GTT induced liver injury and its synergism and toxicity reducing mechanisms, as well as the preventive measures were discussed, thus providing evidence-based basis for safe clinical application of GTT.
Animals
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Chemical and Drug Induced Liver Injury
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Drug Interactions
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Glucosides
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pharmacology
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toxicity
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Liver
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drug effects
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Tripterygium
4.Enhanced absorption and inhibited metabolism of emodin by 2, 3, 5, 4'-tetrahydroxystilbene-2-O-β-D-glucopyranoside: Possible mechanisms for Polygoni Multiflori Radix-induced liver injury.
Qiong YU ; Li-Long JIANG ; Na LUO ; Ya-Xi FAN ; Jiang MA ; Ping LI ; Hui-Jun LI
Chinese Journal of Natural Medicines (English Ed.) 2017;15(6):451-457
Polygoni Multiflori Radix (PMR) has been commonly used as a tonic in China for centuries. However, PMR-associated hepatotoxicity is becoming a safety issue. In our previous in vivo study, an interaction between stilbenes and anthraquinones has been discovered and a hypothesis is proposed that the interaction between stilbene glucoside-enriching fraction and emodin may contribute to the side effects of PMR. To further support our previous in vivo results in rats, the present in vitro study was designed to evaluate the effects of 2, 3, 5, 4'-tetrahydroxystilbene-2-O-β-D-glucopyranoside (TSG) on the cellular absorption and human liver microsome metabolism of emodin. The obtained results indicated that the absorption of emodin in Caco-2 cells was enhanced and the metabolism of emodin in human liver microsomes was inhibited after TSG treatment. The effects of the transport inhibitors on the cellular emodin accumulation were also examined. Western blot assay suggested that the depressed metabolism of emodin could be attributed to the down-regulation of UDP-glucuronosyltransferases (UGTs) 1A8, 1A10, and 2B7. These findings definitively demonstrated the existence of interaction between TSG and emodin, which provide a basis for a better understanding of the underlying mechanism for PMR-induced liver injury.
Caco-2 Cells
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Chemical and Drug Induced Liver Injury
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etiology
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Emodin
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analysis
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metabolism
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Fallopia multiflora
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adverse effects
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Glucosides
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toxicity
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Glucuronosyltransferase
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antagonists & inhibitors
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Humans
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Plant Roots
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Stilbenes
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toxicity
5.Experimental studies on toxic effects of Tiangou Jiangya capsule.
Ying CHEN ; Yujie LI ; Qing YANG ; Xiaoyang WENG ; Lijuan ZOU ; Xiaoxin ZHU
China Journal of Chinese Materia Medica 2011;36(23):3358-3363
OBJECTIVETo study the acute and chronic toxicity of Tiangou Jiangya capsule.
METHODTiangou Jiangya capsule was intragastrically administered to mice twice a day. The appearance, behavior, mental status, respiratory changes of mice and the number of poisoned and dead mice in each group were noted daily for 14 consecutive days. The amount of weight and feed of survived mice were recorded every day. The mice were divided into four groups: the treatment groups (minimum, middle, maximum dose of Tiangou Jiangya capsule) and the control group. After continuously orally administrated for 6 months, the rats' behavior, weight gain, food consumption, indications for hematology, blood biochemistry, urine analysis, electrocardiogram, systematic autopsy and histopathology were observed. The above physiological indexes were inspected again 1 month after cease of administration.
RESULTThe oral acute toxicity study of Tiangou Jiangya capsule in mice revealed that the maximum dose is 534.86 g x kg(-1), which was 534.86 times the recommended human maximum dose in clinical practice. Compared with normal control group, no significant differences were observed in rats' behaviors, food-intake, electrocardiogram and relative examination indexes among the treatment groups. There was no difference of hematology, biochemistry test, urine and histopathology.
CONCLUSIONThe minimum dosage of Tiangou Jiangya capsule is relatively safe. It caused weight loss by administrated with the middle and maximum dose for 6 months, which should be paid attention in clinical studies.
Animals ; Antihypertensive Agents ; administration & dosage ; toxicity ; Behavior, Animal ; drug effects ; Benzyl Alcohols ; administration & dosage ; toxicity ; Body Weight ; drug effects ; Drugs, Chinese Herbal ; administration & dosage ; toxicity ; Eating ; drug effects ; Electrocardiography ; drug effects ; Female ; Flavonoids ; administration & dosage ; toxicity ; Furans ; administration & dosage ; toxicity ; Glucosides ; administration & dosage ; toxicity ; Lignans ; administration & dosage ; toxicity ; Male ; Mice ; Mice, Inbred ICR ; Rats ; Rats, Wistar
6.Antioxidant mechanism of gastrodin combined with isorhynchophylline in inhibiting MPP~+-induced apoptosis of PC12 cells.
Xu LI ; Tian-Jiao XU ; Li-Kun LIU ; Miao-Xian DONG
China Journal of Chinese Materia Medica 2021;46(2):420-425
Gastrodiae Rhizoma-Uncariae Ramulus cum Uncis is the most frequently used herbal pair in the treatment of Parkinson's disease(PD). Gastrodin and isorhynchophylline are important components of Gastrodiae Rhizoma-Uncariae Ramulus cum Uncis herb pair with anti-Parkinson mechanism. This study aimed to investigate the effect of gastrodin combined with isorhynchophylline on 1-methyl-4-phenylpyridinium(MPP~+)-induced apoptosis of PC12 cells and their antioxidant mechanism. The leakage of lactate dehydrogenase(LDH) from cells to media was analyzed by spectrophotometry. Apoptotic cells were labeled with Annexin V-fluorescein isothiocyanate(FITC) and propidium iodide(PI) and analyzed by flow cytometry. The cell cycle was analyzed using propidium iodide(PI) staining. Lipid peroxidation(LPO) level was analyzed by spectrophotometry. The mRNA expression of caspase-3 was examined by Real-time RT-PCR. The protein expressions of heme oxygenase 1(HO-1) and NADPH: quinoneoxidore-ductase 1(NQO-1) were determined by Western blot. Gastrodin combined with isorhynchophylline reduced the percentage of Annexin V-positive cells and cell cycle arrest in MPP~+-induced PC12 cells. Gastrodin combined with isorhynchophylline down-regulated the mRNA expression of caspase-3, up-regulated the protein expressions of HO-1 and NQO-1, and reduced LPO content in MPP~+-induced PC12 cells. PD98059, LY294002 or LiCl could partially reverse these changes pretreated with gastrodin combined with isorhynchophylline, suggesting that gastrodin combined with isorhynchophylline inhibited MPP~+-induced apoptosis of PC12 cells and oxidative stress through ERK1/2 and PI3 K/GSK-3β signal pathways. Our experiments showed that gastrodin combined with isorhynchophylline could down-re-gulate the mRNA expression of caspase-3 and up-regulate the protein expressions of HO-1 and NQO-1, so as to reduce oxidative stress and inhibit apoptosis.
1-Methyl-4-phenylpyridinium/toxicity*
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Animals
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Antioxidants
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Apoptosis
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Benzyl Alcohols
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Cell Survival
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Glucosides
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Glycogen Synthase Kinase 3 beta
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Oxindoles
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PC12 Cells
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Rats
7.Primary safety evaluation of sulfated paeoniae radix alba.
Shan-Jun HUANG ; Rui WANG ; Yan-Hong SHI ; Li YANG ; Zai-Yong WANG ; Zheng-Tao WANG
Acta Pharmaceutica Sinica 2012;47(4):486-491
The paper is to report the development of a method of quantitative analysis of multi-components by high performance liquid chromatography (HPLC) for simultaneously determining paeoniflorin sulfonate (PS), paeoniflorin (PF) and albiflorin (AF) in sulfated Paeoniae Radix Alba. Moreover, the cytotoxicity of paeoniflorin sulfonate by MTT-assay and the acute toxicity of mice by administration of paeoniflorin sulfonate were evaluated. Chromatographic separation of paeoniflorin sulfonate, PF and AF were performed on a SHISEIDO CAPCELL PAK C18 column (250 mm x 4.6 mm, 5 microm) for HPLC and a mixture of acetonitrile and 0.02% phosphoric acid solution (15 : 85) as the mobile phase. As detector a spectrophotometer set at 230 nm; column temperature 30 degrees C; flow rate 1.0 mL x min(-1). The toxicity of paeoniflorin sulfonate was evaluated by in vitro cytotoxicity carried out on mouse and human primary hepatocytes, and by acute oral toxicity test carried out on mice. The calibration curve of paeoniflorin sulfonate, PF and AF revealed linearity in the range of 0.041 8 - 1.045 0, 0.023 5 - 0.587 5, and 0.039 8 - 0.995 0 mg x mL(-1), respectively (r > 0.999 8). The average recovery was ranged from 99.11% to 101.71%, RSD < 2%. Paeoniflorin sulfonate does not have any cytotoxicity to cells at all the tested concentrations (< or = 300 micromol x L(-1)) in the in vitro cytotoxicity assay. The maximum tolerance dose of paeoniflorin sulfonate solution and extraction of Paeoniae Radix Alba to mouse is 5 g x kg(-1) and 80 g x kg(-1) respectively. The contents of these three components in the samples were determined with the developed method. It is a rapid, convenient and accurate method to determine multi-components. The content of PF in sulfated Paeoniae Radix Alba is significantly lower, and there is negative correlationship between the content of paeoniflorin sulfonate and PF. The in vitro cytotoxicity assay and in vivo mouse acute toxicity test showed that there is no obvious toxicity of paeoniflorin sulfonate and water-soluble extract of sulfated Paeoniae Radix Alba.
Animals
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Benzoates
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analysis
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isolation & purification
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toxicity
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Bridged-Ring Compounds
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analysis
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isolation & purification
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toxicity
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Chromatography, High Pressure Liquid
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Glucosides
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analysis
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isolation & purification
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toxicity
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Hepatocytes
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drug effects
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Humans
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Mice
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Mice, Inbred ICR
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Monoterpenes
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Paeonia
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chemistry
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drug effects
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Plant Roots
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chemistry
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drug effects
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Plants, Medicinal
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chemistry
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drug effects
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Sulfur
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pharmacology
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Volatilization
8.Cycloartane triterpenoid and its glucoside isolated from Cassia occidentalis.
Chinese Journal of Natural Medicines (English Ed.) 2017;15(12):950-954
In the present study, one new cycloartane triterpenoid, named cycloccidentalic acid C (1) and its glucoside, cycloccidentaliside VI (2) were isolated from the whole plant of Cassia occidentalis. Their structures were elucidated by a combinational analyses of 1D and 2D NMR data and HRMS. Compound 2 showed modest anti-HIV-1 activity with EC value of 1.44 μmol·L and TI (Therapeutic Index) value of 15.59.
Anti-HIV Agents
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isolation & purification
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pharmacology
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Cell Line, Tumor
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Cell Survival
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drug effects
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Glucosides
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chemistry
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pharmacology
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toxicity
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Humans
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Inhibitory Concentration 50
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Magnetic Resonance Spectroscopy
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Molecular Structure
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Plant Extracts
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chemistry
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isolation & purification
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pharmacology
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toxicity
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Senna Plant
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chemistry
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Triterpenes
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chemistry
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isolation & purification
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pharmacology
;
toxicity
9.Effects of polydatin on bleomycin-induced pulmonary fibrosis in rats.
Yan ZHANG ; Liyan CHEN ; Biao LIANG
China Journal of Chinese Materia Medica 2011;36(24):3528-3534
OBJECTIVETo observe the effects of three different doses of polydatin (PD) on pulmonary interstitial fibrosis in rats induced by bleomycin.
METHODOne hundred and twenty-nine healthy Sprague-Dawley rats three months old, were randomly divided into six groups. Group A: normal control group; group B: model group treated with bleomycin (pretreatment with saline 1 mL x kg(-1) intraperitoneally before bleomycin); group C: PD 10 mg x kg(-1) (pretreatment with PD 10 mg x kg(-1) intraperitoneally before bleomycin); group D: PD 20 mg x kg(-1) (pretreatment with PD 20 mg x kg(-1) intraperitoneally before bleomycin); group E: PD 40 mg x kg(-1) (pretreatment with PD 40 mg x kg(-1) intraperitoneally before bleomycin), group F: dexamethason (DXM) treated group (pretreatment with saline 1 mL x kg(-1) intraperitoneally before bleomycin and then with DXM 1 mg x kg(-1) x d(-1)). At day 3, 7, 14, 28 after injection of bleomycin, eight rats in each group were randomly chosen to be killed. The right lungs of dead rats were removed and appropriately processed for hematoxylin and eosin (H&E) stain, histologically observed under light microscope. The hydroxyproline content and the PLA2 activity in pulmonary homogenate were measured with alkaline hydrolysis assay and acid modified microtitrimetic method. The levels of leukotriene C4 (LTC4), prostaglandin E2 (PGE2), transforming growth factor-beta1 (TGF-beta1) in bronchoalveolar lavage fluid (BALF) were measured with enzyme-linked immunosorbent assay (ELISA).
RESULTAt day 3, 7, 14, 28 after intratracheal instillation of bleomycin in rats of group B, the PLA2 activity in lung homogenate and the levels of its metabolic products PGE2, LTC4 as well as TGF-beta1 in BALF increased significantly compared with those in group A (P < 0.01). And lung hydroxyproline concentration began to grow up markedly at day 7 compared with those in group A (P < 0.05), reaching its maximum at day 28. Compared with group B, three different doses of PD and DXM significantly reduced the activity of the PLA2 and hydroxyproline concentration in lung homogenate as well as the levels of PGE2, LTC4, TGF-beta1 in BALF at various periods (P < 0.05). There was statistically significant difference between three different doses of PD groups (P < 0.05). And the group E (PD 40 mg x kg(-1)) was lower than group D (PD 20 mg x kg(-1)), group D was lower than group C (PD 10 mg x kg(-1)) (respectively, P < 0.01). Group E and DXM group were no significant difference. However, all these observation parameters were higher than the normal level (compared with group A, P < 0.01). Histological studies revealed that it was showed less inflammation and a lower degree of fibrosis in the lungs treated with PD than bleomycin model group.
CONCLUSIONPD has the protective effect on pulmonary interstitial fibrosis. However, it can't completely block the process of pulmonary fibrosis.
Animals ; Bleomycin ; toxicity ; Dinoprostone ; analysis ; Drugs, Chinese Herbal ; therapeutic use ; Female ; Glucosides ; therapeutic use ; Leukotriene C4 ; analysis ; Male ; Phospholipases A2 ; analysis ; Pulmonary Fibrosis ; chemically induced ; drug therapy ; metabolism ; Rats ; Rats, Sprague-Dawley ; Stilbenes ; therapeutic use
10.Comparative study on effects of blood enriching on mouse model of blood deficiency syndrome induced by cyclophosphamide of albiflorin, paeoniflorin on levels of GM-CSF, IL-3 and TNF-α.
Ying-li ZHU ; Jian-jun ZHANG ; Jing-xia WANG ; Zhi-hui YANG ; Yin-feng HUANG ; Sheng-sheng QU ; Tian-tian ZHOU
China Journal of Chinese Materia Medica 2015;40(2):330-333
OBJECTIVETo compare the effects and mechanism of blood enriching on mouse model of blood deficiency syndrome induced by cyclophosphamide of albiflorin and paeoniflorin.
METHODAlbiflorin and paeoniflorin were determined by using animal models of blood deficiency syndrome induced by cyclophosphamide. The amount of WBC, RBC, HGB, index of thymus gland and spleen, and the changes of GM-CSF, IL-3 and TNF-α in serum were detected after the treatment.
RESULTCompared with the model group, the amount of WBC in the group of 30 mg x kg(-1) albiflorin and 30 mg x kg(-1) paeoniflorin were increased obviously (P < 0.01). The amount of RBC in the group of 30 mg x kg(-1) albiflorin and 30 mg x kg(-1) paeoniflorin were increased obviously (P < 0.01, P < 0.001), which did not had a significant difference compared with the same dose. The index of thymus gland in the group of 30 mg x kg(-1) albiflorin was superior to the model group (P < 0.01), the difference was significant compared with the same dose of paeoniflorin (P < 0.05). The GM-CSF in serum in all groups of 30 mg x kg(-1) albiflorin, 15 mg x kg(-1) albiflorin, 30 mg x kg(-1) paeoniflorin and 15 mg x kg(-1) paeoniflorin increased obviously (P < 0.01, P < 0.05, P < 0.01, P < 0.05); The IL-3 in serum in both group of 30 mg x kg(-1) albiflorin and 30 mg x kg(-1) paeoniflorin also increased (P < 0.001). The content of TNF-α in group of 30 mg x kg(-1) albiflorin and 30 mg x kg(-1) paeoniflorin were reduced (P < 0.01), which showed the obvious difference compared with the same dose group (P < 0.01).
CONCLUSIONAlbiflorin had the effect of blood enriching by regulating the immune function, same with the paeoniflorin. The probable mechanism of nourishing blood and liver of Paeoniae Radix Alba was not only the better effect of adjusting the content of TNF-α, but also might act synergistically with paeoniflorin.
Animals ; Blood Cells ; drug effects ; Bridged-Ring Compounds ; pharmacology ; Cyclophosphamide ; toxicity ; Glucosides ; pharmacology ; Granulocyte-Macrophage Colony-Stimulating Factor ; blood ; Hematopoiesis ; drug effects ; Interleukin-3 ; blood ; Male ; Mice ; Monoterpenes ; pharmacology ; Tumor Necrosis Factor-alpha ; blood