1.HIV - 1 p24 Expression in BCG and the Immunogenicity of Recombinant BCG in Experimental Animals.
Sang Hyun CHO ; Han Jeong MYOUNG ; Hye Rhan JEONG ; Gil Han BAI ; Sang Jae KIM ; Yong Soo BAE
Korean Journal of Immunology 1997;19(2):289-300
HIV-1 p24 was cloned into multiple cloning site of pMV261, extrachromosomal expression vectors carrying BCG replication origin and BCG-specific heat-shock promoter, and then introduced into BCG and E. coli. Western blot experiments showed that the p24 efficiently expressed in recombinant BCG (rBCG), but not in E. coli. Recombinant p24 expression induced by a single heat-shock of rBCG was maintained longer than 3 weeks. Immunoblot experiments with intact rBCG did not show any distinctive positive signal, suggesting that the recombinant protein was not secreted or exposed at the surface of BCG. The guinea pigs immunized with live rBCG showed delayed type hypersensitivity (DTH) by the systemic area as well as an effective humoral immunity, suggesting that tbis rBCG is believed to elicit eKcient immune responses against p24, even though the expression is restricted only in the cytoplasm as reported previously with other antigen. These results demonstrate that BCG can be developed as a live recombinant vaccine vector against a broad spectrum of infectious disease.
Animals*
;
Blotting, Western
;
Clone Cells
;
Cloning, Organism
;
Communicable Diseases
;
Cytoplasm
;
Guinea Pigs
;
HIV*
;
HIV-1
;
Hypersensitivity
;
Immunity, Humoral
;
Mycobacterium bovis*
;
Replication Origin
2.Characteristics of Dizziness in Supratentorial Infarctions.
Yeong Bae SEO ; Jung Hwan YUN ; Dong Jin SHIN ; Yeong Bae LEE ; Kyu Cheol HAN
Journal of the Korean Neurological Association 2009;27(1):7-12
BACKGROUND: Dizziness due to brain lesions manifests mainly in infratentorial lesions, with few cases related to supratentorial lesions having been reported. This study aimed to elucidate the clinical characteristics and demographic factors of patients with dizziness caused by cerebral infarction and to determine the site of the brain where supratentorial lesions are most prevalent. METHODS: Patients with prominent dizziness who visited the emergency room of Gachon University Gil Hospital between July 2006 and July 2007 were included. Among them, 101 patients with acute cerebral infarction were categorized into supratentorial (n=51) and infratentorial (n=50) groups based on brain MRI. Demographics and clinical characteristics of dizziness in each group were compared, and common brain sites of the supratentorial group were assessed. RESULTS: The nature of the dizziness differed between the supratentorial group (vertigo, 27.4%; presyncope, 5.9%; disequilibrium, 29.4%; ocular, 11.8%; and nonspecific, 25.5%) and the infratentorial group (vertigo, 50.0%; presyncope, 6.0%; disequilibrium, 32.0%; ocular, 0%; and nonspecific, 12.0%; p=0.02). The duration of dizziness was shorter in the supratentorial than the infratentorial group (p<0.01). In the supratentorial group, common sites of the lesion were the thalamus (19.6%) and frontoparietal lobe (15.7%). The lesion usually appeared in the left hemisphere (60.8%). CONCLUSIONS: Dizziness from supratentorial lesions manifests in different ways, and its duration is shorter than that from infratentorial lesions. The central vestibular pathway may be located in the thalamus and frontoparietal lobe.
Brain
;
Cerebral Infarction
;
Demography
;
Dizziness
;
Emergencies
;
Humans
;
Infarction
;
Syncope
;
Thalamus
3.Minimally invasive facial rejuvenation combining thread lifting with liposuction: A clinical comparison with thread lifting alone
Kwang Il BAE ; Dong Gil HAN ; Sung Eun KIM ; Young Bae LEE
Archives of Aesthetic Plastic Surgery 2019;25(2):52-58
BACKGROUND: Barbed thread lifting is known to be a minimally invasive procedure compared with standard incisional surgery for facial rejuvenation. However, some studies have pointed out that the effects of thread lifting do not last long and disappear after several months. Therefore, the aim of this study was to evaluate the efficiency and duration of anchoring-type absorbable thread lifting combined with liposuction for facial rejuvenation. METHODS: A retrospective review of 27 cosmetic patients who underwent absorbable barbed thread lifting with liposuction between July 2013 and December 2015 was undertaken. The efficiency of facial rejuvenation was evaluated through a patient satisfaction score and the global aesthetic improvement scale (GAIS). RESULTS: Subjects were divided into two groups: thread lifting only and thread lifting combined with liposuction. The mean follow-up period of each group was 8.5 and 9.3 months, respectively. In the thread lifting-only group, the average patient satisfaction score was 2.36 (1=unsatisfied to 4=very satisfied), and the average GAIS score was 2.54 (1=very much improved to 5=worsen). In the thread lifting with liposuction group, the average patient satisfaction score was 3.06, and the average GAIS score was 1.85. CONCLUSIONS: Thread lifting combined with liposuction showed better patient satisfaction and GAIS results than thread lifting alone, even over a long follow-up period.
Follow-Up Studies
;
Humans
;
Lifting
;
Lipectomy
;
Patient Satisfaction
;
Rejuvenation
;
Retrospective Studies
;
Suture Anchors
4.Early Statins after Intravenous or Endovascular Recanalization Is Beneficial Regardless of Timing, Intensity, and Stroke Mechanism.
Han Gil JEONG ; Beom Joon KIM ; Mi Hwa YANG ; Moon Ku HAN ; Hee Joon BAE
Journal of Stroke 2017;19(3):370-372
No abstract available.
Hydroxymethylglutaryl-CoA Reductase Inhibitors*
;
Stroke*
5.Factors Associated with Disclosure of Sexual Abuse of Children and Adolescent Victims.
Jung Soo HAN ; Seong Jin CHO ; Seung Min BAE
Journal of the Korean Academy of Child and Adolescent Psychiatry 2015;26(3):176-182
OBJECTIVES: The purpose of this study was to analyze the factors related to the disclosure patterns of sexual abuse in children and adolescent victims. METHODS: A sample of 153 children and adolescents who visited the Sunflower children's Center (Incheon) between January 1, 2011 and December 31, 2012 were analyzed. The medical records of the victims were reviewed retrospectively. RESULTS: Ninety (58.8%) subjects made a disclosure of sexual abuse voluntarily. The revelation was most commonly made to a parent. The severity and duration of sexual abuse were related to the disclosure patterns. In addition, the group that displayed below average intelligence, particularly subjects in the group with borderline intellectual functioning, tended to disclose their history of abuse less voluntarily than the subjects with normal intelligence. CONCLUSION: The current study provides insight into the factors that affect disclosure patterns in children and adolescent sexual abuse victims. To prevent sexual abuse, we suggest that special legal assistance and social concern be required for children and adolescents with borderline intellectual functioning.
Adolescent*
;
Child Abuse, Sexual
;
Child*
;
Disclosure*
;
Helianthus
;
Humans
;
Intelligence
;
Medical Records
;
Parents
;
Retrospective Studies
;
Sex Offenses*
6.A Case of Myasthenia Gravis Associated with Recurrent Transverse Myelitis.
Dong Chul HAN ; Dong Jin SHIN ; Tae Yong KIM ; Yeong Bae LEE
Journal of the Korean Neurological Association 2007;25(2):244-246
Myasthenia gravis is an acquired autoimmune disorder characterized clinically by the weakness of skeletal muscles and fatigability on exertion. Autoimmune diseases such as systemic lupus erythematosus, autoimmune thyroiditis, rheumatoid arthritis, Sj?gren syndrome and polymyositis may be associated with myasthenia gravis. However, there are only a few reports of transverse myelitis with myasthenia gravis. We report a case of myasthenia gravis with recurrent transverse myelitis.
Arthritis, Rheumatoid
;
Autoimmune Diseases
;
Lupus Erythematosus, Systemic
;
Muscle, Skeletal
;
Myasthenia Gravis*
;
Myelitis, Transverse*
;
Polymyositis
;
Thyroiditis, Autoimmune
7.Early Complications from Absorbable Anchoring Suture Following Thread-Lift for Facial Rejuvenation.
Seung Hun YEO ; Young Bae LEE ; Dong Gil HAN
Archives of Aesthetic Plastic Surgery 2017;23(1):11-16
BACKGROUND: Thread lifting has been known as a minimally invasive procedure compared with the standard incisional surgery for facial rejuvenation. Although there have been several reports on acute or delayed complications after using nonabsorbable thread type, there have only been a few studies on such complications after using absorbable thread type. Hence, the aim of this study was to introduce various complications of thread lifting using the absorbable anchoring type for facial rejuvenation and to discuss about how to resolve it. METHODS: A retrospective review of 144 cosmetic patients, who underwent absorbable barbed thread lifting between July 2013 and December 2015, was performed. The procedure was done using the temporal anchoring technique. The thread used in this study are polydioxanone molding cog (PMC) and polydioxanone cutting cog (PCC), which are both the absorbable type. RESULTS: The results are as follows: Dimple was developed in 3 cases (2.1%); thread exposure was developed in 5 cases (3.5%); alopecia was developed in 3 cases (2.1%); undercorrection was developed in 3 cases (2.1%); asymmetry was developed in 1 case (0.7%); and parotid gland injury was developed in 1 case (0.7%). Out of the total 144 patients, sixteen (11.1%) patients developed at least 1 complication. There was no detectable inflammation or consistent skin excavation. CONCLUSIONS: Most complications of absorbable thread lifting are minor ones, and such complications resolve by retouching or widening the anchoring gap, but it is important to keep in mind the possibility of parotid gland injury.
Alopecia
;
Fungi
;
Humans
;
Inflammation
;
Lifting
;
Parotid Gland
;
Polydioxanone
;
Rejuvenation*
;
Retrospective Studies
;
Rhytidoplasty
;
Skin
;
Suture Anchors
;
Sutures*
8.Prevention of Virus - induced Diabetes by Single Immunization with Recombinant BCG in SJL/J Male Mice.
Sang Hyun CHO ; Yong Kyung CHOE ; Gil Han BAI ; Sang Jae KIM ; Yong Soo BAE ; Beom Kyu CHOI ; Byung Hwa HYUN ; Hyung Hoan LEE
Korean Journal of Immunology 1999;21(3):259-269
D variant of encephalomyocarditis (EMC-D) virus causes diabetes in susceptible mice by direct infection and cytolysis of pancreatic beta cells. cDNA covering the major outer capsid protein (VP1) of EMC-D virus was cloned into Mycobacterium bovis bacillus Calmette-Guerin (BCG). None of the SJL/J male mice, immunized with live recombinant BCG-VP1, became diabetic when challenged with highly diabetogenic EMC-D virus. But the control mice inoculated with normal BCG or rBCG transformed with vector alone developed diabetes in the same challenge. VP1-specific antibodies including neutralizing antibodies were markedly increased as time went on and reached to the maximum titer at week 10 after a single immunization. The plateau of the titer lasted longer than following 4 weeks. Guinea pigs immunized with the live rBCG-VP1 showed strong delayed type hypersensitivity (DTH) to the VP1of EMC-D virus. It means that the live rBCG-VP1 elicit efficient humoral and cell-mediated imrnune responses against EMC-D virus, resulting in prevention of virus-induced diabetes in susceptible mice.
Animals
;
Antibodies
;
Antibodies, Neutralizing
;
Bacillus
;
Capsid Proteins
;
Clone Cells
;
DNA, Complementary
;
Guinea Pigs
;
Humans
;
Hypersensitivity
;
Immunization*
;
Insulin-Secreting Cells
;
Male*
;
Mice*
;
Mycobacterium bovis*
9.Facial Nerve Paralysis after General Anesthesia: A case report .
Young Jung YOON ; Hun Gil YIM ; Han Suck KIM ; Hong Youl KIM ; In Bae LEE
Korean Journal of Anesthesiology 1998;34(5):1055-1059
A 42-year-old male was scheduled for left fronto-temporo-parietal craniotomy and subdural hematoma removal under general anesthesia. The past history was diabetes mellitus, hypertension, and chronic alcoholism. He was moderately obese, short neck, and deep drowsy. After rapid sequence induction with fentanyl, thiopental sodium and succinylcholine, anesthesia was maintained with nitrous oxide-oxygen-isoflurane. His head was fixed on horseshoe head rest with right side tilted. Throughout the 4 hours of anesthesia, cardiovascular and respiratory variables remained within normal limit. After extubation, upper respiratory obstruction developed, presumably as the result of the tongue's falling back against the posterior pharyngeal wall. This was only partly corrected by insertion of a rubber oral airway, but it was fully relieved when the lower jaw was lifted forward by bilateral digital pressure applied behind the angles of the mandible about ten minutes. At the intensive care unit, left partial facial nerve paresis developed with mild weakness of the right corner of the mouth, and striking right parotid swelling and tenderness. The parotid swelling receded over 2 weeks, and the paresis resolved over 3 weeks to complete recovery.
Adult
;
Alcoholism
;
Anesthesia
;
Anesthesia, General*
;
Craniotomy
;
Diabetes Mellitus
;
Facial Nerve*
;
Fentanyl
;
Head
;
Hematoma, Subdural
;
Humans
;
Hypertension
;
Intensive Care Units
;
Jaw
;
Male
;
Mandible
;
Mouth
;
Neck
;
Paralysis*
;
Paresis
;
Rubber
;
Strikes, Employee
;
Succinylcholine
;
Thiopental
10.Two Cases of Cutaneous Infections due to Mycobacterium marinum.
Mo Kyu SUH ; Jang Seok BANG ; Yeol Oh SUNG ; Ki Seong YOON ; Gyoung Yim HA ; Jung Ran KIM ; Gil Han BAE
Korean Journal of Dermatology 1998;36(5):932-937
We report two cases of cutaneous Mycobacterium(M.) marinum infections in a 43-year-old man and a 31-year-old woman who had multiple subcutaneous nodules on the left upper extremity and an erythematous ulcer on the right elbow, respectively. Skin biopsy specimens revealed chronic granulamatous inflammation and AFB positive bacilli in the dermis. The culture from tissue specimens on Lowenstein-Jensen media at 30'C for 10 days, yielded cream-colored colonies, which became yellow when exposed to light. The organism was identified as M. marinum by selected biochemical reactions. The patients were treated with surgical excision followed by administration of minocycline and administration of minocycline, respectively.
Adult
;
Biopsy
;
Dermis
;
Elbow
;
Female
;
Humans
;
Inflammation
;
Minocycline
;
Mycobacterium marinum*
;
Mycobacterium*
;
Skin
;
Ulcer
;
Upper Extremity