Study on blood samples of 29 patients with hemophilia A and 25 normal people to determine the mutation of intron 18 and 19 of factor VIII by PCR-RFLP using Vent DNA polymerase assay. PCR and RFLP methods were used. Results: 16/29 (55%) of haemophilia A patients has the mutation in intron 18 of factor VIII gene and none of them has the mutation in intron 19. This PCR-RFLP assay can be applied for detection of mutations and these methods can be used for carrier analysis and prenatal diagnosis of hemophilia A.
Hemophilia A ; Polymerase Chain Reaction ; DNA

Beta-Thalassemia is a blood disease with beta-globin deficiency caused by a directly down-regulation in the synthesis of structurally normal beta chains. Vent DNA polymerase is a high-fidelity thermophilic DNA polymerase, and the fidelity 5 - 15 times higher than Taq DNA polymerase (1/31,000 vs. 1/290 - 1/2,400). Using ARMS PCR (Amplification Refractor Mutation System-PCR) with Vent DNA polymerase, we found that 9/28 (32%) of the tested beta-Thalassemia patients had the mutation at codon 17 and 4/28 (14%) at codon 41/42. This method can be applied for a rapid prenatal diagnosis of beta-Thalassemia disease and has public health significance.
beta-Thalassemia ; DNA ; Polymerase Chain Reaction ; Diagnosis

On experimental rats, the effect of pain relieve, of neuroleptic and acute toxicity of Formula D1 was studies. The formula composes of 9 componants (Bach truat, Hoang ky, Chi thuc, Moc huong, Nghe, Cam thao, Lac tien, Bach thuoc, Huong phu). It manifests an effects of analgesic on white mouse, experimented with the method of heat plate and Koster. The formula inhibits Central nervous system, it has not acute toxicity when administered orally in dose of 73 folds of therapeutic dose
Chemistry, Pharmaceutical ; Medicine, Traditional ; Therapeutics ; drugs

Cot khi cu was studied on experiment animals. Pain relief effect was evaluated with 1% extract, sedative and hypnotic effect was compared with hexobarbital and acute toxicity was assessed by Lichfield Wilcozon Method. Results showed that raw and processed Cot khi cu did not cause acute toxicity, with a dose of 200 folds of clinical dose. In raw and processed Cot khi cu, there is no defference in pain relief effect. Roast Cot khi cu gave a better sedative effect than the raw Cot khi cu
Acute Toxicity Tests ; Animals ; Biochemistry ; drugs

The anti-flammatory effect of Drynaria fortunei was studied on rat’s hind paw edema induced by carrageenin. Results showed that its aqueous extract in the dose of 4g/kg body weight exerts anti-inflammatory effect. Acute effect of Drynaria fortunei is exhibited through the decreased quantity of exudation and the number of leucocyte in the exudation.
Anti-Inflammatory Agents ; Animal Experimentation ; Medicine, Traditional

The effect of analgetic and anti inflammatory of Tinospora Sinensis Men were studies in the form liquid glue on experimental mice. These mice were divided into 4 groups: group 1 takes NaCl 0,9%; group 2 takes Aspegic; group 3 takes liquid glue with dose 4g/kg; group 4 takes liquid glue with dose 8g/kg. The results were: after 5 min causing pain, aspegin and Tinospora Sinensis Men relieves pain clearly. After 15 min, these two drugs also has effect vigorous (58.88 – 88.5%) compared to control group with P<0.001. Dose 4g/kg of Tinospora Sinensis Men had not effected yet acute anti inflammatory clearly. Dose higher 8g/kg can inhibit inflammatory reaction on leg of mice with 10,16% (P<0.005), less than aspegic 0.05g/kg. The conclusion: Tinospora Sinensis Men has effect relieving pain by peripheral machanism, anti inflammatory acute and relievring oedematose reaction local with dose 8g/kg
drugs ; Medicine, Traditional ; Pain ; Anti-Inflammatory Agents

Study on acute and subchronic toxicity of CP preparation (extracted from Cu Binh Voi) finding using CP preparation with 1000mg/kg mouse of dose, follow in 72 hours, not any mice died. With 30mg/kg mouse uninterrupted take in 30 days, CP preparation didn’t effect to making blood function (but reduced number of leukocyte). It exchanged effect of eliminate urea thourgh renal. It not exchanged tranxaminaza level in serum but slightly effect to sterilize function of rabit
Medicine, Traditional ; Biochemistry ; Plant Extracts

Study on ‘Cot toai bo’ (Drynaria bonii Rhizome) product was processed from fresh stem and root of Drynaria bonii Christ., collected at Lang Son from July 2003 to September 2003. Results: chemical analysis showed that Cot toai bo contains flavonoids, steroid saponins, sterols, tannins, deoxidized sugar. Total flavonoid in stem and root accounted for 0.60±0.03% and total saponin was 3.71±0.06%. After processing by traditional method, total flavonoids and saponins in processed materials reduced in comparison with raw material. Compare 2 processing methods: in rice alcohol processed material, whose chemical constituents were reduced very little but in fried material, whose chemical constituents were reduced more than raw material. Rice alcohol processed Cot toai bo had analgesic and anti-inflammatory effects better than raw material and this processing was a more appropriate method than fried method
Polypodiaceae ; Rhizome ; Chemistry

A 51 year old female with left breast carcinoma underwnet mastectomy, radiotherapy and has had chemotherapy for two years. Chest X-ray (CXR) film showed the linear pattern in the peripheri of both lungs. High resolution computed tomography (HRCT) images defined reticulo-nodullar opacities in lower zone of the lung bilaterally consistant with pulmonary lymphangitic carcinomatosis. In summary, HRCT of the lung is a modality can define and characterize difuse lung lesions, in particularly, pulmonary lymphangitic carcinomatosis.
Lung Neoplasms ; Tomography, X-Ray Computed

The study was carried out with healthy white mice of the strain of Swiss, weighed 202 g, fed properly in good supply of water and food. Results a pain relief and narcotic effects of the material. An intensive dose 200 times higher than usual clinical dose did not manifest toxicity in the animal
Medicine, Traditional ; Chemistry, Pharmaceutical ; Acute Toxicity Tests