D1S80 locus is highly polymorphic and has been used worldwide as an important marker for forensic, medical analysis and paternity tests. Vent DNA Polymerase is a high-fidelity thermophilic DNA polymerase. The fidelity of Vent DNA polymerase is 5 to 15 folds higher than that observed for Tag DNA polymerase. This high fidelity derives in part from an integral 3’-5’ proofreading exonuclease activity in Vent DNA polymerase. Greater than 90% of the polymerase activity remains after one hour incubation at 95oC. Because of these advantages, we want to apply Vent DNA polymerase for the amplification of D1S80 alleles by PCR. Our study showed that PCR condition for Vent DNA polymerase are rather different than for Tag DNA polymerase. The amplification of D1S80 alleles with Vent DNA polymerase is optimal with the following parameters: 1. A final concentration of MgSO4 in PCR mixture of 4 mM. 2. An annealing temperature for the specific D1S80 primers 68oC. 3. A final concentration of dNTPs of 200mcM.
DNA polymerase ; Alleles

Deep vent DNA polymerase is purified from a strain of E. Coli that carries the Deep Vent DNA polymerase gene from pyrococcus species GB-D. The native organism was isolated from a submarine thermal vent at 2010 meters and is able to grow at temperature as high as 104oC. Deep Vent DNA polymerase is a high-fidelity thermophilic DNA polymerase. The Deep Vent DNA polymerase is derived from an integral 3'-5' proofreading exonuclease activity. Deep Vent DNA polymerase is ven more stable than Vent DNA polymerase at temperature of 95 to 100oC. Because of these advantages, we want to apply Deep Vent DNA polymerase for amplification of NS4 gene - hepatitis C virus by PCR. Our study showed that PCR conditions for Deep Vent DNA polymerase are rather different than for Taq DNA polymerase. The amplification of NS4 gene - hepatitis C virus with Deep Vent DNA polymerase is optimal with the following parameters: (1) A final concentration of MgSO4 in PCR mixture of 4 mM. (2) An annealing temperature of 57oC. (3) A final concentration of dNTPs of 200 mM.
Hepatitis C ; Deep Vent DNA polymerase

Authors implemented the study to find characteristics of Doppler ultrasonic images in patients with HCC. This is a prospective study and carried out in B¹ch Mai Hospital. In the study on 15 patients, who had positive diagnosis of HCC, were selected. All of them underwent Doppler ultrasound and hepatic arteriography. Intra-tumoral and peri-tumoral flow is found with Doppler U.S 86.6% of tumors compared to the results of hepatic arteriography. The average peak velocity of hepatic artery and intratumoral artery is 83.3 and 57.2 cm/s respectively. The resistive Index is from 0.4 to 0.88 in intratumoural vessels.
Carcinoma, Hepatocellular ; Angiography ; Ultrasonics

Subjects: liquid extraction (1/1) of acaranthus; frog; dog; rabbit; mice. Method: effects of extraction on blood pressure of dog (effects on adrenaline and nicotine), on isolated heart of frog, on the narcotic effect of hexobarbital in mice. Results: Liquid extraction has synergic effect with hexobarbital, rapid and long hypotension because of adrenaline blocking (a partly block of gland of autonomic nervous system) and vasodilator
Hypertension ; Antihypertensive Agents

Healthy rats with weight of 120-180 g were randomly divided into 4 groups. They tested the anti-acute inflamatory activity of T1 (3 -methoxy 4 hydroxy-5 bromobenzylidien para bromo anilin) and T2 (3-methoxy 4 hydroxy-5 nitrobenzylidien p-nitro anilin). The results have shown that T1 had a significant antiinflamatory activity after 2 h and 4 h of the test but had no antiflamatory activity after 6 h of the test comparing with the control. While the anti-inflamatory activity of T2 was not different from this in the control. This study concluded that the anti-inflamatory activity of derivatives of 5 bromo vanilin was better than this of derivatives of 5 -nitroanilin.
Anti-Inflammatory Agents ; analogs & derivatives

Study on blood samples of 29 patients with hemophilia A and 25 normal people to determine the mutation of intron 18 and 19 of factor VIII by PCR-RFLP using Vent DNA polymerase assay. PCR and RFLP methods were used. Results: 16/29 (55%) of haemophilia A patients has the mutation in intron 18 of factor VIII gene and none of them has the mutation in intron 19. This PCR-RFLP assay can be applied for detection of mutations and these methods can be used for carrier analysis and prenatal diagnosis of hemophilia A.
Hemophilia A ; Polymerase Chain Reaction ; DNA

Beta-Thalassemia is a blood disease with beta-globin deficiency caused by a directly down-regulation in the synthesis of structurally normal beta chains. Vent DNA polymerase is a high-fidelity thermophilic DNA polymerase, and the fidelity 5 - 15 times higher than Taq DNA polymerase (1/31,000 vs. 1/290 - 1/2,400). Using ARMS PCR (Amplification Refractor Mutation System-PCR) with Vent DNA polymerase, we found that 9/28 (32%) of the tested beta-Thalassemia patients had the mutation at codon 17 and 4/28 (14%) at codon 41/42. This method can be applied for a rapid prenatal diagnosis of beta-Thalassemia disease and has public health significance.
beta-Thalassemia ; DNA ; Polymerase Chain Reaction ; Diagnosis

On experimental rats, the effect of pain relieve, of neuroleptic and acute toxicity of Formula D1 was studies. The formula composes of 9 componants (Bach truat, Hoang ky, Chi thuc, Moc huong, Nghe, Cam thao, Lac tien, Bach thuoc, Huong phu). It manifests an effects of analgesic on white mouse, experimented with the method of heat plate and Koster. The formula inhibits Central nervous system, it has not acute toxicity when administered orally in dose of 73 folds of therapeutic dose
Chemistry, Pharmaceutical ; Medicine, Traditional ; Therapeutics ; drugs

Cot khi cu was studied on experiment animals. Pain relief effect was evaluated with 1% extract, sedative and hypnotic effect was compared with hexobarbital and acute toxicity was assessed by Lichfield Wilcozon Method. Results showed that raw and processed Cot khi cu did not cause acute toxicity, with a dose of 200 folds of clinical dose. In raw and processed Cot khi cu, there is no defference in pain relief effect. Roast Cot khi cu gave a better sedative effect than the raw Cot khi cu
Acute Toxicity Tests ; Animals ; Biochemistry ; drugs

The anti-flammatory effect of Drynaria fortunei was studied on rat’s hind paw edema induced by carrageenin. Results showed that its aqueous extract in the dose of 4g/kg body weight exerts anti-inflammatory effect. Acute effect of Drynaria fortunei is exhibited through the decreased quantity of exudation and the number of leucocyte in the exudation.
Anti-Inflammatory Agents ; Animal Experimentation ; Medicine, Traditional