1.One case of correlation between the human herpesvirus 7 DNA load and the hemo hagocytic syndrome
Peng CAI ; Chun-Rong TONG ; Jun-Fang YANG ; Ying LIU ; Guo-Lin XU ; Gian-Feng ZHANG ; Cheng-Liang ZHEN ; Gun-Fang LI ; Zhi-Ping TENG
Chinese Journal of Experimental and Clinical Virology 2011;25(2):123-125
Objective To research the relationship between human herpesvirus 7 (HHV-7) viral Load and the etiopathogenisis of hemophagocytic syndrome, in order to provide evidence for the clinical diagnosis of hemophagocytic syndrome and anti-virus therapy. Methods Peripheral blood of patient with hemophagocytic syndrome during different treatment periods, extracted DNA, Syntheticed the primers of HHV-7,gene sequence of PCR amplified fragments detected, determined HHV-7 viral Load by Real - time fluorescent quantitative PCR and the ferritin concentration in peripheral blood detected by chemiluminescence. Result The sequence result indicated that PCR amplified fragment was a part of HHV-7 gene, the ferritin concentration viried with the load of HHV-7. Conclusion The occurrence of hemophagocytic syndrome is connetted with the load of HHV-7.
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